LOCUS       62056_22660        7929 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    204..260
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     rep_origin      complement(769..1357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1531..2388)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2389..2493)
                     /label=AmpR promoter
     CDS             complement(2660..4033)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
                     LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
                     HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
                     ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
                     MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
                     GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
                     "
     CDS             complement(4236..5237)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     promoter        5662..5690
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 aatgacaaat ggtccaaact agtactaata aaattaatca ttttgaaagc gcaaacaaag
       61 ttttatacga aggtaaagat tctaaaaatc ctttagcttt taaatactat aaccctgaag
      121 aagtagtagg cggtaaaacg atgaaagatc agctgcgttt ttctgttgct tactggcacc
      181 agtttacagc agatggtacg gatcaattcg agctcggtac ccggggatcc tctagagtcg
      241 acctgcaggc atgcaagctt tcgcgagctc gagatctaga tatcgatgaa ttgatccgac
      301 gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc
      361 cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg
      421 atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg tcacggcgat
      481 ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata
      541 ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat
      601 ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc
      661 ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc
      721 atctccagca gccgcacgcg gcgcatctcg ggccgcgttg ctggcgtttt tccataggct
      781 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      961 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1201 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1261 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1321 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1381 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1441 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1501 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1561 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1621 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     1681 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1741 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1801 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
     1861 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1921 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1981 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2041 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2101 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
     2161 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2221 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2281 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2341 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2401 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2461 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     2521 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     2581 ctttcgtctt caagaattcc tgttataaaa aaaggatcaa ttttgaactc tctcccaaag
     2641 ttgatccctt aacgatttag aaatcccttt gagaatgttt atatacattc aaggtaacca
     2701 gccaactaat gacaatgatt cctgaaaaaa gtaataacaa attactatac agataagttg
     2761 actgatcaac ttccataggt aacaaccttt gatcaagtaa gggtatggat aataaaccac
     2821 ctacaattgc aatacctgtt ccctctgata aaaagctggt aaagttaagc aaactcattc
     2881 cagcaccagc ttcctgctgt ttcaagctac ttgaaacaat tgttgatata actgttttgg
     2941 tgaacgaaag cccacctaaa acaaatacga ttataattgt catgaaccat gatgttgttt
     3001 ctaaaagaaa ggaagcagtt aaaaagctaa cagaaagaaa tgtaactccg atgtttaaca
     3061 cgtataaagg acctcttcta tcaacaagta tcccaccaat gtagccgaaa ataatgacac
     3121 tcattgttcc agggaaaata attacacttc cgatttcggc agtacttagc tggtgaacat
     3181 ctttcatcat ataaggaacc atagagacaa accctgctac tgttccaaat ataattcccc
     3241 cacaaagaac tccaatcata aaaggtatat ttttccctaa tccgggatca acaaaaggat
     3301 ctgttacttt cctgatatgt tttacaaata tcaggaatga cagcacgcta acgataagaa
     3361 aagaaatgct atatgatgtt gtaaacaaca taaaaaatac aatgcctaca gacattagta
     3421 taattccttt gatatcaaaa tgacctttta tccttacttc tttctttaat aatttcataa
     3481 gaaacggaac agtgataatt gttatcatag gaatgagtag aagataggac caatgaatat
     3541 aatgggctat cattccacca atcgctggac cgactccttc tcccatggct actatcgatc
     3601 caataagacc aaatgcttta cccctatttt cctttggaat atagcgcgca actacaacca
     3661 ttacgagtgc tggaaatgca gctgcaccag ccccttgaat aaaacgagcc ataataagta
     3721 aggaaaagaa agaatggcca acaaacccaa ttaccgaccc gaaacaattt attataattc
     3781 caaataggag taaccttttg atgcctaatt gatcagatag ctttccatat acagctgttc
     3841 caatggaaaa ggttaacata aaggctgtgt tcacccagtt tgtactcgca ggtggtttat
     3901 taaaatcatt tgcaatatca ggtaatgaga cgttcaaaac catttcattt aatacgctaa
     3961 aaaaagataa aatgcaaagc caaattaaaa tttggttgtg tcgtaaattc gattgtgaat
     4021 aggatgtatt cacatttcac cctccaataa tgagggcaga cgtagtttat agggttaatg
     4081 atacgcttcc ctcttttaat tgaaccctgt tacattcatt acacttcata attaattcct
     4141 cctaaacttg attaaaacat tttaccacat ataaactaag ttttaaattc agtatttcat
     4201 cacttataca acaatatggc ccgtttgttg aactactctt taataaaata atttttccgt
     4261 tcccaattcc acattgcaat aatagaaaat ccatcttcat cggctttttc gtcatcatct
     4321 gtatgaatca aatcgccttc ttctgtgtca tcaaggttta attttttatg tatttctttt
     4381 aacaaaccac cataggagat taacctttta cggtgtaaac cttcctccaa atcagacaaa
     4441 cgtttcaaat tcttttcttc atcatcggtc ataaaatccg tatcctttac aggatatttt
     4501 gcagtttcgt caattgccga ttgtatatcc gatttatatt tatttttcgg tcgaatcatt
     4561 tgaactttta catttggatc atagtctaat ttcattgcct ttttccaaaa ttgaatccat
     4621 tgtttttgat tcacgtagtt ttctgtattc ttaaaataag ttggttccac acataccaat
     4681 acatgcatgt gctgattata agaattatct ttattattta ttgtcacttc cgttgcacgc
     4741 ataaaaccaa caagattttt attaattttt ttatattgca tcattcggcg aaatccttga
     4801 gccatatctg acaaactctt atttaattct tcgccatcat aaacattttt aactgttaat
     4861 gtgagaaaca accaacgaac tgttggcttt tgtttaataa cttcagcaac aaccttttgt
     4921 gactgaatgc catgtttcat tgctctcctc cagttgcaca ttggacaaag cctggattta
     4981 caaaaccaca ctcgatacaa ctttctttcg cctgtttcac gattttgttt atactctaat
     5041 atttcagcac aatcttttac tctttcagcc tttttaaatt caagaatatg cagaagttca
     5101 aagtaatcaa cattagcgat tttcttttct ctccatggtc tcacttttcc actttttgtc
     5161 ttgtccacta aaacccttga tttttcatct gaataaatgc tactattagg acacataata
     5221 ttaaaagaaa cccccatcta tttagttatt tgtttggtca cttataactt taacagatgg
     5281 ggtttttctg tgcaaccaat tttaagggtt ttcaatactt taaaacacat acataccaac
     5341 acttcaacgc acctttcagc aactaaaata aaaatgacgt tatttctata tgtatcaaga
     5401 taagaaagaa caagttcaaa accatcaaaa aaagacacct tttcaggtgc tttttttatt
     5461 ttataaactc attccctgat ctcgacttcg ttcttttttt acctctcggt tatgagttag
     5521 ttcaaattcg ttctttttag gttctaaatc gtgtttttct tggaattgtg ctgttttatc
     5581 ctttaccttg tctacaaacc ccttaaaaac gtttttaaag gcttttaagc cgtctgtacg
     5641 ttccttaagg aattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt
     5701 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat
     5761 cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact
     5821 gccgggcctc ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct
     5881 gctagcgcta tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga
     5941 ccgctttggc cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc
     6001 gatcatggcg accacacccg tcctgtggat ctgacgcgtg taactgcgaa ggtaaaacgg
     6061 gtgattgaaa ataaactaac aaatgaagag gaacgaaaga gaagtctaga atttgttacg
     6121 tttatatcgg atgaattact gcaaaatgat acggagcctc gcacatttat tattcagaac
     6181 tctcttttat cgctagagaa aatccatact ctcgcacaga ccaaagaggt agaggaatat
     6241 aaagaagtca taactactct gacaagacat gattcataat ttgaaaagca ggtaaactaa
     6301 cccaaaaggc aagactctcc gtagtttaga aaagagcagg ttgctaataa catataaaca
     6361 gccagttgcc gttatgatag gtgactggct gcatgggatg aaaaggtgag ggtggagaca
     6421 gacataacac tcttaataga agagggtaat tctttctctt ttatagaaaa tcaattaatt
     6481 gaaagtagct ccttcattct taagatcaac gtgatatagg tttgctaacc tttgcgttca
     6541 cttaactaac ttataggggt aacacttaaa aaagaatcaa taacgataga aaccgctcct
     6601 aaagcaggtg cattttttcc taacgaagaa ggcaatagtt cacatttatt gtctaaatga
     6661 gaatggactc tagaagaaac ttcgttttta atcgtattta aaacaatggg atgagattca
     6721 attatatgat ttctcaagat aacagcttct atatcaaatg tattaaggat attggttaat
     6781 ccaattccga tataaaagcc aaagttttga agtgcattta acatttctac atcattttta
     6841 tttgcgcgtt ccacaatctc ttttcgagaa atattctttt cttctttaga gagcgaagcc
     6901 agtaacgctt tttcagaagc atataattcc caacagcctc gatttccaca gctgcatttg
     6961 ggtccattaa aatctatcgt catatgaccc atttccccag aaaaaccctg aacaccttta
     7021 tacaattcgt tgttaataac aagtccagtt ccaattccga tattaatact gatgtaaacg
     7081 atgttttcat agttttttgt cataccaaat actttttcac cgtatgctcc tgcattagct
     7141 tcattttcaa caaaaaccgg aacattaaac tcactctcaa ttaaaaactg caaatctttg
     7201 atattccaat ttaagttagg catgaaaata atttgctgat gacgatctac aaggcctgga
     7261 acacaaattc ctattccgac tagaccataa ggggactcag gcatatgggt tacaaaacca
     7321 tgaataagtg caaataaaat ctcttttact tcactagcgg aagaactaga caagtcagaa
     7381 gtcttctcga gaataatatt tccttctaag tcggttagaa ttccgttaag atagtcgact
     7441 cctatatcaa taccaatcga gtagcctgca ttcttattaa aaacaagcat tacaggtctt
     7501 ctgccgcctc tagattgccc tgccccaatt tcaaaaataa aatctttttc aagcagtgta
     7561 tttacttgag aggagacagt agacttgttt aatcctgtaa tctcagagag agttgccctg
     7621 gagacagggg agttcttcaa aatttcatct aatattaatt tttgattcat tttttttact
     7681 aaagcttgat ctgcaatttg aataataacc actcctttgt ttatccaccg aactaagttg
     7741 gtgttttttg aagcttgaat tagatattta aaagtatcat atctaatatt ataactaaat
     7801 tttctaaaaa aaacattgaa ataaacattt attttgtata tgatgagata aagttagttt
     7861 attggataaa caaactaact caattaagat agttgatgga taaacttgtt cacttaaatc
     7921 aaaggggga
//
