LOCUS       40924_46513        8179 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWEB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Fiandt M, Jendrisak J, Meis R.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-1998) R
REFERENCE   2  (bases 1 to 8179)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8179)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-JUN-1998) R"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     12..35
                     /label=M13 forward primer binding site
                     /note="M13 forward primer binding site"
     promoter        complement(54..72)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        94..122
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     misc_feature    460..935
                     /label=lambda phage cos site
                     /note="lambda phage cos site"
     promoter        3287..3616
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3973..4764
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     intron          5185..5250
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5380..5400
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    5825..5959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6355..6943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7117..7974)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7975..8079)
                     /label=AmpR promoter
ORIGIN
        1 gaattcgcgg ccgccagggt tttcccagtc acgacggatc ccgggatcct ctccctatag
       61 tgagtcgtat tatgcggccg cgaattctca tgtttgaccg cttatcatcg ataagcttta
      121 atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa
      181 caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt
      241 tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca
      301 ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg
      361 agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac
      421 tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atctgcctcg ctggcctgcc
      481 gcagttcttc aacctcccgg cgcagctttt cgttctcaat ttcagcatcc ctttcggcat
      541 accattttat gacggcggca gagtcataaa gcacctcatt acccttgcca ccgcctcgca
      601 gaacgggcat tccctgttcc tgccagttct gaatggtacg gatactcgca ccgaaaatgt
      661 cagccagctg ctttttgttg acttccattg ttcattccac ggacaaaaac agagaaagga
      721 aacgacagag gccaaaaagc tcgctttcag cacctgtcgt ttcctttctt ttcagagggt
      781 attttaaata aaaacattaa gttatgacga agaagaacgg aaacgcctta aaccggaaaa
      841 ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt aacaaggcgg atcgccggaa
      901 aggacccgca aatgataata attatcaatt gcatactatc gacggcactg ctgccagata
      961 acaccaccgg ggaaacattc catcatgatg gccgtgcgga cataggaagc cagttcatcc
     1021 atcgctttct tgtctgctgc catttgcttt gtgacatcca gcgccgcaca ttcagcagcg
     1081 tttttcagcg cgttttcgat caacgtttca atgttggtat caacaccagg tttaactttg
     1141 aacttatcgg cactgacggt taccttgttc tgcgctggct catcacgctg gataccaagg
     1201 ctgatgttgt agatattggt caccggctga ggtgtttcga ttgccgctgc gtggatagca
     1261 ccatttgcga tagcggcgtc cttgatgaat gacactccat tgcgaataag ttcgaaggag
     1321 acggtgtcac gaatgcgctg gtccagctcg tcgattgcct tttgtgcagc agaggtatca
     1381 atctcaacgc caagcgtcat cgaagcgcaa tattgctgct caccaaaacg cgtattgacc
     1441 aggtgttcaa cggcaaattt ctgcccttct gatgtcagaa aggtaaagtg attttctttc
     1501 tggtattcag ttgctgtgtg tctggtttca gcaaaaccaa gctcgcgcaa ttcggctgtg
     1561 ccagatttag aaggcagatc accagacagc aacgcgccac ggaaaaacag cgcatacaga
     1621 acatccgtcg ccgcgccgga caacgtgata attttatgac ccatgattta tttcctttta
     1681 gacgtgagcc tgtcgcacag caaagccgcc gaaagttaac ggtttgccca ggctcacaac
     1741 tgaaagactt tctacggtgt gcgcgtgcga tgcgcgtaga agactgattt atcaacctgt
     1801 ctttatatca ggattcatta cctgactatt tgtgggtaaa gttcgtagtg cgctgatcgt
     1861 gcaaaatgat tttagttggg aacagttcgc aactctgtcc cataaaaatc agcatattcc
     1921 catctatccc atatccagcg cattgaccat cgggatactg aagggagatt ccatcatctc
     1981 ttagaaagat caccatctct tttgtttcaa tttgcatata gctacctgga ggatttatga
     2041 atacaaggat tttcatggac tattaccatg agattgattt tccatcttta ttcgcgagag
     2101 cagtggaaag cgatgacgat gtgggtacta cattgcgcat tcacctactt tgtgagcgca
     2161 tggtcgaagc atggatatgc gcatgctgtg actgccaaga tcctctacgc cggacgcatc
     2221 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     2281 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     2341 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     2401 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     2461 cataagggag agcgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     2521 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     2581 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     2641 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     2701 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     2761 gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc
     2821 cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg
     2881 tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc
     2941 agcctaactt cgatcattgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc
     3001 acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg
     3061 ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg
     3121 ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg
     3181 cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc
     3241 ggcgcatctc gggcagcgtt gggtcctgca gatccggctg tggaatgtgt gtcagttagg
     3301 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3361 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3421 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3481 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3541 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3601 cctaggcttt tgcaaaaagc ttcacgctgc cgcaagcact cagggcgcaa gggctgctaa
     3661 aggaagcgga acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
     3721 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc
     3781 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa
     3841 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
     3901 ttcttgccgc caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga
     3961 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4021 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4081 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4141 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4201 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4261 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4321 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4381 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4441 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4501 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4561 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     4621 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     4681 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     4741 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     4801 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     4861 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     4921 tggagttctt cgcccaccgg gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg
     4981 ctggacgacc tcgcggagtt ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc
     5041 agcggctatc cgcgcatcca tgcccccgaa ctgcaggagt ggggaggcac gatggccgct
     5101 ttggtccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
     5161 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
     5221 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
     5281 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
     5341 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
     5401 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     5461 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
     5521 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
     5581 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
     5641 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
     5701 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     5761 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     5821 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5881 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5941 atcttatcat gtctggatct gacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
     6001 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
     6061 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
     6121 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ctcttccgct tcctcgctca
     6181 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6301 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6361 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6421 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6481 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6541 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6601 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6661 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6721 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6781 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6841 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6901 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6961 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7021 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7081 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7141 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7201 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7261 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7321 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7381 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     7441 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7501 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7561 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7621 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7681 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     7741 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7801 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7861 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7921 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7981 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8041 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8101 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     8161 gaggcccttt cgtcttcaa
//
