LOCUS       40924_46218        6454 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pWB106, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Bourn WR, Shephard E, Powels R, Stutz H, Douglas N, Jaffray A, 
            Williamson A-L.
  TITLE     The use of guided evolution to create a stable, immunogenic HIV-1 
            antigen expressing recombinant BCG
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Shephard E, Bourn WR, Stutz H, Johnston N, van Harmelen JH, 
            Williamson C, Douglass N, Bowers D, Galant S, Isaacs Z, Binder A, 
            Williamson A-L.
  TITLE     Priming with a rBCG or DNA vaccine expressing HIV-I subtype C Gag 
            elicits comparable CD8+ T cell responses when boosted by a rMVA 
            expressing GagC
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6454)
  AUTHORS   Bourn WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2007) Medical Virology and Institute of Infectious
            Disease and Molecular Medicine, University of Cape Town, Anzio Road 
            Observatory, Cape Town 7925, South Africa
REFERENCE   4  (bases 1 to 6454)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6454)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (10-JAN-2007) Medical Virology and Institute of Infectious Disease 
            and Molecular Medicine, University of Cape Town, Anzio Road 
            Observatory, Cape Town 7925, South Africa"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2014..3909
                     /note="mycobacterial origin; OriM"
     regulatory      3936..4022
                     /label=BCG hsp60
                     /note="BCG hsp60"
                     /regulatory_class="promoter"
     regulatory      4046..4052
                     /note="strong synthetic Shine Delgarno sequence; RBSA"
                     /regulatory_class="ribosome_binding_site"
     gene            4089..4451
                     /gene="tat"
                     /label=tat
                     /note="shuffled BCG codon optimized tat gene from HIV-1
                     subtype C isolate"
     gene            4461..4883
                     /gene="gag"
                     /label=gag
                     /note="truncated fragment of BCG codon optimized gag gene
                     from HIV-1 subtype C isolate"
     regulatory      4768..4774
                     /gene="gag"
                     /label=fortuitous RBSD
                     /note="fortuitous RBSD"
                     /regulatory_class="ribosome_binding_site"
     gene            4886..5539
                     /gene="gag"
                     /label=gag
                     /note="truncated fragment of BCG codon optimized gag gene
                     from HIV-1 subtype C isolate"
     misc_feature    5597..5626
                     /label=Gag-GFP linker sequence
                     /note="Gag-GFP linker sequence"
     CDS             5624..6337
                     /label=Cycle 3 GFP
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
     terminator      6372..6418
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagcaaca aagcgacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
       61 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      121 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      181 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      241 atcgcttgta tgggaagccc catgcgccag agttgtttct gaaacatggc aaaggtagcg
      301 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      361 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      421 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      481 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      541 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      601 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      661 aaatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
      721 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
      781 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
      841 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
      901 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
      961 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     1021 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1081 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1141 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1201 ttcacgaggc agacctcact agttccactg agcgtcagac cccgtagaaa agatcaaagg
     1261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1381 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1621 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     1681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1981 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgagcc caccagctcc gtaagttcgg
     2041 gtgctgtgtg gctcgtaccc gcgcattcag gcggcagggg gtctaacggg tctaaggcgg
     2101 cgtgtacggc cgccacagcg gctcttagcg gcccggaaac gtcctcgaaa cgacgcatgt
     2161 gttcctcctg gttggtacag gtggttgggg gtgctcggct gtcgctggtg tttcatcatc
     2221 agggctcgac gggagagcgg gggagtgtgc agttgtgggg tggcccctca gcgaaatatc
     2281 tgacttggag ctcgtgtcgg accatacacc ggtgattaat cgtggtttat tatcaagcgt
     2341 gagccacgtc gccgacgaat ttgagcagct ctggctgccg tactggtccc tggcaagcga
     2401 cgatctgctc gaggggatct accgccaaag ccgcgcgtcg gccctaggcc gccggtacat
     2461 cgaggcgaac ccaacagcgc tggcaaacct gctggtcgtg gacgtagacc atccagacgc
     2521 agcgctccga gcgctcagcg cccgggggtc ccatccgctg cccaacgcga tcgtgggcaa
     2581 tcgcgccaac ggccacgcac acgcagtgtg ggcactcaac gcccctgttc cacgcaccga
     2641 atacgcgcgg cgtaagccgc tcgcatacat ggcggcgtgc gccgaaggcc ttcggcgcgc
     2701 cgtcgatggc gaccgcagtt actcaggcct catgaccaaa aaccccggcc acatcgcctg
     2761 ggaaacggaa tggctccact cagatctcta cacactcagc cacatcgagg ccgagctcgg
     2821 cgcgaacatg ccaccgccgc gctggcgtca gcagaccacg tacaaagcgg ctccgacgcc
     2881 gctagggcgg aattgcgcac tgttcgattc cgtcaggttg tgggcctatc ttcccgccct
     2941 catgcggatc tacctgccga cccggaacgt ggacggactc ggccgcgcga tctatgccga
     3001 gtgccacgcg cgaaacgccg aatttccgtg caacgacgtg tgtcccggac cgctaccgga
     3061 cagcgaggtc cgcgccatcg ccaacagcat ttggcgttgg atcacaacca agtcgcgcat
     3121 ttgggcggac gggatcgtgg tctacgaggc cacactcagt gcgcgccatg cggccatctc
     3181 gcggaagggc gcagcagcgc gcacggcggc gagcacagtt gcgcggcgcg caaagtccgc
     3241 gtcagccatg gaggcattgc tatgagcgac ggctacagcg acggctacag cgacggctac
     3301 aactggcagc cgactgtccg caaaaagcgg cgcgtgaccg ccgccgaagg cgctcgaatc
     3361 accggactat ccgaacgcca cgtcgtccgg ctcgtggcgc aggaacgcag cgagtggttc
     3421 gccgagcagg ctgcacgccg cgaacgcatc cgcgcctatc acgacgacga gggccactct
     3481 tggccgcaaa cggccaaaca tttcgggctg catctggaca ccgttaagcg actcggctat
     3541 cgggcgagga aagagcgtgc ggcagaacag gaagcggctc aaaaggccca caacgaagcc
     3601 gacaatccac cgctgttcta acgcaattgg ggagcgggtg tcgcgggggt tccgtggggg
     3661 gttccgttgc aacgggtcgg acaggtaaaa gtcctggtag acgctagttt tctggtttgg
     3721 gccatgcctg tctcgttgcg tgtttcgttg cgtccgtttt gaataccagc cagacgagac
     3781 ggggttctac gaatcttggt cgataccaag ccatttccgc tgaatatcgt ggagctcacc
     3841 gccagaatcg gtggttgtgg tgatgtacgt ggcgaactcc gttgtagtgc ttgtggtggc
     3901 atccgtggcg cggccgcggt accagatctt taaatctaga caaggtcgaa cgaggggcat
     3961 gacccggtgc ggggcttctt gcactcggca taggcgagtg ctaagaataa cgttggcact
     4021 cgaattcgag ttaggtacgt gacttaagga ggcaaaattc catgggttac gtatcgtcga
     4081 cccaagctct gggcatctcg tacggccgca agaagcgccg ccagcgccgc tcgaccccgc
     4141 cgtcgtcgga ggaccaccag aacccgatct cgaagcagcc gctgccgcag acccgcggcg
     4201 acccgaccgg ctcggaggag tcgaagaaga aggtcgagag caagaccaag accgacccgt
     4261 tcgactgcaa gtactgctcg taccactgcc tggtgtgctt ccagaccaag ggcctcggta
     4321 tctcctacgg gcggaagaag cggatggagc ccatcgaccc gaacctggag ccctggaacc
     4381 acccgggctc gcagccgaac accccgtgca acaagtgcta ttgcaaatac tgctcctatc
     4441 actgcctcgt cgtcgaagct gcccgcgcct cgatcctgcg cggcgagaag ctggacaagt
     4501 gggagaagat ccgcctgcgc ccgggcggca agaagcacta catgctgaag cacatcgtgt
     4561 gggcctcgcg cgagctggag cgcttcgccc tgaacccgga cctgctggag acctcggagg
     4621 gctgcaagca gatcatgaag cagctccagc cggccctcca gaccggcacc gagaagctca
     4681 agtcgctgta caacaccgtg gccaccctgt actgcgtgca cgagaagatc gaggtgcgcg
     4741 acaccaagga ggccctggac aagatcgagg aggagcagaa caagtgccag caaaagaccc
     4801 agcaggccaa ggccgccgac ggcaaggtgt cgcagaacta cccgattatg cagaacctcc
     4861 agggccagat ggtgcaccag gcctctcgcc gcgcaccctg aacgcctggg tgaaggtgat
     4921 cgaggagaag gcgttctcgc cggaggtgat cccgatgttc accgccctgt cggagggtgc
     4981 caccccgcag gacctgaaca ccatgctgaa caccgtgggc ggccaccagg ccgccatgca
     5041 gatgctgaag gacaccatca acgaggaggc cgccgagtgg gaccgcctgc acccggtgca
     5101 cgccggcccg attgccccgg gccagatgcg cgagccgcgc ggctcggaca tcgccggcac
     5161 cacctcgacc ctccaggagc agatcgcctg gatgacctcg aacccgccga tcccggtggg
     5221 cgacatctac aagcgctgga tcatcctggg cctgaacaag atcgtgcgca tgtactcgcc
     5281 ggtgtcgatc ctggacatcc gccagggtcc gaaggagccg ttccgcgact acgtggaccg
     5341 cttcttcaag accctgcgcg ccgagcaggc cacccaggag gtgaagaact ggatgaccga
     5401 caccctgctg gtgcagaacg ccaacccgga ctgcaagacc atcctgcgcg ccctgggtcc
     5461 gggtgccacc ctggaggaga tgatgaccgc ctgccagggc gtgggcggcc cgggccacaa
     5521 ggcccgcgtg ctggccgaag gccatgtcgc agaccaactc gggcaacatc atgatgcagc
     5581 gctcgaactt caagggtccg gctccgccgg ttccgccggc tcagcttcga aaggagaaga
     5641 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     5701 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
     5761 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttctctta
     5821 tggtgttcaa tgcttttcaa gatacccaga tcatatgaag cggcacgact tcttcaagag
     5881 cgccatgcct gagggatacg tgcaggagag gaccatctct ttcaaggacg acgggaacta
     5941 caagacacgt gctgaagtca agtttgaggg agacaccctc gtcaacagga tcgagcttaa
     6001 gggaatcgat ttcaaggagg acggaaacat cctcggccac aagttggaat acaactacaa
     6061 ctcccacaac gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa
     6121 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     6181 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     6241 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     6301 tgctgggatt acacatggca tggatgaact atacaaatga gttaactagc gtacgatcga
     6361 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     6421 gttgtttgtc cggccatcat ggccgcggtg atca
//
