LOCUS       40924_46213        6791 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pWB105, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Bourn WR, Shephard E, Powels R, Stutz H, Douglas N, Jaffray A, 
            Williamson A-L.
  TITLE     The use of guided evolution to create a stable, immunogenic HIV-1 
            antigen expressing recombinant BCG
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Bourn WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2007) Medical Virology and Institute of Infectious
            Disease and Molecular Medicine, University of Cape Town, Anzio Road 
            Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 6791)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JAN-2007) Medical Virology and Institute of Infectious Disease 
            and Molecular Medicine, University of Cape Town, Anzio Road 
            Observatory, Cape Town 7925, South Africa"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2014..3909
                     /note="mycobacterial origin; OriM"
     regulatory      3936..4022
                     /label=BCG hsp60
                     /note="BCG hsp60"
                     /regulatory_class="promoter"
     regulatory      4046..4052
                     /note="strong synthetic Shine Delgarno sequence; RBSA"
                     /regulatory_class="ribosome_binding_site"
     gene            4089..4451
                     /gene="tat"
                     /label=tat
                     /note="shuffled BCG codon optimized tat gene from HIV-1
                     subtype C isolate"
     gene            4461..5930
                     /gene="gag"
                     /label=gag
                     /note="BCG codon optimized gag gene from HIV-1 subtype C 
                     isolate"
     misc_feature    5931..5963
                     /label=Gag-GFP linker sequence
                     /note="Gag-GFP linker sequence"
     CDS             5961..6674
                     /label=Cycle 3 GFP
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
     terminator      6709..6755
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagcaaca aagcgacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
       61 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      121 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      181 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      241 atcgcttgta tgggaagccc catgcgccag agttgtttct gaaacatggc aaaggtagcg
      301 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      361 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      421 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      481 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      541 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      601 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      661 aaatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
      721 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
      781 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
      841 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
      901 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
      961 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     1021 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1081 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1141 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1201 ttcacgaggc agacctcact agttccactg agcgtcagac cccgtagaaa agatcaaagg
     1261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1381 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1621 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     1681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1981 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgagcc caccagctcc gtaagttcgg
     2041 gtgctgtgtg gctcgtaccc gcgcattcag gcggcagggg gtctaacggg tctaaggcgg
     2101 cgtgtacggc cgccacagcg gctcttagcg gcccggaaac gtcctcgaaa cgacgcatgt
     2161 gttcctcctg gttggtacag gtggttgggg gtgctcggct gtcgctggtg tttcatcatc
     2221 agggctcgac gggagagcgg gggagtgtgc agttgtgggg tggcccctca gcgaaatatc
     2281 tgacttggag ctcgtgtcgg accatacacc ggtgattaat cgtggtttat tatcaagcgt
     2341 gagccacgtc gccgacgaat ttgagcagct ctggctgccg tactggtccc tggcaagcga
     2401 cgatctgctc gaggggatct accgccaaag ccgcgcgtcg gccctaggcc gccggtacat
     2461 cgaggcgaac ccaacagcgc tggcaaacct gctggtcgtg gacgtagacc atccagacgc
     2521 agcgctccga gcgctcagcg cccgggggtc ccatccgctg cccaacgcga tcgtgggcaa
     2581 tcgcgccaac ggccacgcac acgcagtgtg ggcactcaac gcccctgttc cacgcaccga
     2641 atacgcgcgg cgtaagccgc tcgcatacat ggcggcgtgc gccgaaggcc ttcggcgcgc
     2701 cgtcgatggc gaccgcagtt actcaggcct catgaccaaa aaccccggcc acatcgcctg
     2761 ggaaacggaa tggctccact cagatctcta cacactcagc cacatcgagg ccgagctcgg
     2821 cgcgaacatg ccaccgccgc gctggcgtca gcagaccacg tacaaagcgg ctccgacgcc
     2881 gctagggcgg aattgcgcac tgttcgattc cgtcaggttg tgggcctatc ttcccgccct
     2941 catgcggatc tacctgccga cccggaacgt ggacggactc ggccgcgcga tctatgccga
     3001 gtgccacgcg cgaaacgccg aatttccgtg caacgacgtg tgtcccggac cgctaccgga
     3061 cagcgaggtc cgcgccatcg ccaacagcat ttggcgttgg atcacaacca agtcgcgcat
     3121 ttgggcggac gggatcgtgg tctacgaggc cacactcagt gcgcgccatg cggccatctc
     3181 gcggaagggc gcagcagcgc gcacggcggc gagcacagtt gcgcggcgcg caaagtccgc
     3241 gtcagccatg gaggcattgc tatgagcgac ggctacagcg acggctacag cgacggctac
     3301 aactggcagc cgactgtccg caaaaagcgg cgcgtgaccg ccgccgaagg cgctcgaatc
     3361 accggactat ccgaacgcca cgtcgtccgg ctcgtggcgc aggaacgcag cgagtggttc
     3421 gccgagcagg ctgcacgccg cgaacgcatc cgcgcctatc acgacgacga gggccactct
     3481 tggccgcaaa cggccaaaca tttcgggctg catctggaca ccgttaagcg actcggctat
     3541 cgggcgagga aagagcgtgc ggcagaacag gaagcggctc aaaaggccca caacgaagcc
     3601 gacaatccac cgctgttcta acgcaattgg ggagcgggtg tcgcgggggt tccgtggggg
     3661 gttccgttgc aacgggtcgg acaggtaaaa gtcctggtag acgctagttt tctggtttgg
     3721 gccatgcctg tctcgttgcg tgtttcgttg cgtccgtttt gaataccagc cagacgagac
     3781 ggggttctac gaatcttggt cgataccaag ccatttccgc tgaatatcgt ggagctcacc
     3841 gccagaatcg gtggttgtgg tgatgtacgt ggcgaactcc gttgtagtgc ttgtggtggc
     3901 atccgtggcg cggccgcggt accagatctt taaatctaga caaggtcgaa cgaggggcat
     3961 gacccggtgc ggggcttctt gcactcggca taggcgagtg ctaagaataa cgttggcact
     4021 cgaattcgag ttaggtacgt gacttaagga ggcaaaattc catgggttac gtatcgtcga
     4081 cccaagctct gggcatctcg tacggccgca agaagcgccg ccagcgccgc tcgaccccgc
     4141 cgtcgtcgga ggaccaccag aacccgatct cgaagcagcc gctgccgcag acccgcggcg
     4201 acccgaccgg ctcggaggag tcgaagaaga aggtcgagag caagaccaag accgacccgt
     4261 tcgactgcaa gtactgctcg taccactgcc tggtgtgctt ccagaccaag ggcctcggta
     4321 tctcctacgg gcggaagaag cggatggagc ccatcgaccc gaacctggag ccctggaacc
     4381 acccgggctc gcagccgaac accccgtgca acaagtgcta ttgcaaatac tgctcctatc
     4441 actgcctcgt cgtcgaagct gcccgcgcct cgatcctgcg cggcgagaag ctggacaagt
     4501 gggagaagat ccgcctgcgc ccgggcggca agaagcacta catgctgaag cacatcgtgt
     4561 gggcctcgcg cgagctggag cgcttcgccc tgaacccggg cctgctggag acctcggagg
     4621 gctgcaagca gatcatgaag cagctccagc cggccctcca gaccggcacc gaggagctca
     4681 agtcgctgta caacaccgtg gccaccctgt actgcgtgca cgagaagatc gaggtgcgcg
     4741 acaccaagga ggccctggac aagatcgagg aggagcagaa caagtgccag caaaagaccc
     4801 agcaggccaa ggccgccgac ggcaaggtgt cgcagaacta cccgattgtg cagaacctcc
     4861 agggccagat ggtgcaccag gccatctcgc cgcgcaccct gaacgcctgg gtgaaggtga
     4921 tcgaggagaa ggcgttctcg ccggaggtga tcccgatgtt caccgccctg tcggagggtg
     4981 ccaccccgca ggacctgaac accatgctga acaccgtggg cggccaccag gccgccatgc
     5041 agatgctgaa ggacaccatc aacgaggagg ccgccgagtg ggaccgcctg cacccggtgc
     5101 acgccggccc gattgccccg ggccagatgc gcgagccgcg cggctcggac atcgccggca
     5161 ccacctcgac cctccaggag cagatcgcct ggatgacctc gaacccgccg atcccggtgg
     5221 gcgacatcta caagcgctgg atcatcctgg gcctgaacaa gatcgtgcgc atgtactcgc
     5281 cggtgtcgat cctggacatc cgccagggtc cgaaggagcc gttccgcgac tacgtggacc
     5341 gcttcttcaa gaccctgcgc gccgagcagg ccacccagga ggtgaagaac tggatgaccg
     5401 acaccctgct ggtgcagaac gccaacccgg actgcaagac catcctgcgc gccctgggtc
     5461 cgggtgccac cctggaggag atgatgaccg cctgccaggg cgtgggcggc ccgggccaca
     5521 aggcccgcgt gctggccgag gccatgtcgc agaccaactc gggcaacatc atgatgcagc
     5581 gctcgaactt caagggtccg cgccgcatcg tgaagtgctt caactgcggc aaagagggcc
     5641 acatcgcccg caactgccgc gccccgcgca agaagggctg ctggaagtgc ggcaaggagg
     5701 gccaccagat gaaggactgc accgagcgcc aggccaactt cctgggcaag atctggccgt
     5761 cgcacaaggg ccgcccgggc aacttcctcc agaaccgccc ggagccgacc gccccgccgg
     5821 ccgagtcgtt ccgcttcgag gagaccaccc cggccccgaa gcaggagccg attgagcgcg
     5881 agccgctgac ctcgctgaag tcgctgttcg gctcggaccc cctgtcgcaa gcttccggct
     5941 ccgccggttc cgccggctca gcttcgaaag gagaagaact tttcactgga gttgtcccaa
     6001 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
     6061 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
     6121 ctgttccatg gccaacactt gtcactactt tctcttatgg tgttcaatgc ttttcaagat
     6181 acccagatca tatgaagcgg cacgacttct tcaagagcgc catgcctgag ggatacgtgc
     6241 aggagaggac catctctttc aaggacgacg ggaactacaa gacacgtgct gaagtcaagt
     6301 ttgagggaga caccctcgtc aacaggatcg agcttaaggg aatcgatttc aaggaggacg
     6361 gaaacatcct cggccacaag ttggaataca actacaactc ccacaacgta tacatcacgg
     6421 cagacaaaca aaagaatgga atcaaagcta acttcaaaat tagacacaac attgaagatg
     6481 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
     6541 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
     6601 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
     6661 atgaactata caaatgagtt aactagcgta cgatcgactg ccaggcatca aataaaacga
     6721 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtccgg ccatcatggc
     6781 cgcggtgatc a
//
