LOCUS       40924_46188        4861 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWAS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Na D, Yoo SM, Chung H, Park H, Park JH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 4861)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4861)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      292..880
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      1123..1150
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1191..1234
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     ncRNA           complement(1252..1330)
                     /product="micC"
                     /label=antisense_RNA
                     /ncRNA_class="antisense_RNA"
     regulatory      complement(1331..1379)
                     /label=lambda PR promoter
                     /note="lambda PR promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(1399..1415)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(1422..1449)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(1465..1536)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1554..2264)
                     /label=lambda repressor
                     /note="phage lambda repressor"
     promoter        complement(2307..2591)
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             2618..3493
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        3718..3822
                     /label=AmpR promoter
     CDS             3823..4680
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 catatgagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
       61 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
      121 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
      181 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
      241 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      301 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      361 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      421 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
      481 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      541 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      601 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      661 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      721 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      781 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      841 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
      901 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      961 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1021 aacgaccgag cgcagcgagt cagtgagcga ggaacatatg tgacagctta tcgcatgcct
     1081 aggtaaaccc aggaggtata gggcgaattg ggtacctata aacgcagaaa ggcccacccg
     1141 aaggtgagcc agtgtgactc tagtagagag cgttcaccga caaacaacag ataaaacgaa
     1201 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggctcga gaaaaaaagc
     1261 ccggacgact gttcgggctt gtctttttat atgttggaaa atcagtggca atgcaatggc
     1321 ccaacagaaa gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag
     1381 aattcctgca gcccggggga tccactagtt ctagatataa acgcagaaag gcccacccga
     1441 aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga taaaacgaaa
     1501 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctgggaattc ttagccaaac
     1561 gtctcttcag gccactgact agcgataact tcccccacaa cggaacaact ctcattgcat
     1621 gggatcattg ggtactgtgg gtttagtggt tgtaaaaaca cctgaccgct atccctgatc
     1681 agtttcttga aggtaaactc atcaccccca agtctggcta tgcagaaatc acctggctca
     1741 acagcctgct cagggtcaac gagaattaac attccgtcag gaaagcttgg cttggagcct
     1801 gttggtgcgg tcatggaatt accttcaacc tcaagccaga atgcagaatc actggctttt
     1861 ttggttgtgc ttacccatct ctccgcatca cctttggtaa aggttctaag cttaggtgag
     1921 aacatccctg cctgaacatg agaaaaaaca gggtactcat actcacttct aagtgacggc
     1981 tgcatactaa ccgcttcata catctcgtag atttctctgg cgattgaagg gctaaattct
     2041 tcaacgctaa ctttgagaat ttttgcaagc aatgcggcgt tataagcatt taatgcattg
     2101 atgccattaa ataaagcacc aacgcctgac tgccccatcc ccatcttgtc tgcgacagat
     2161 tcctgggata agccaagttc atttttcttt ttttcataaa ttgctttaag gcgacgtgcg
     2221 tcctcaagct gctcttgtgt taatggtttc ttttttgtgc tcatatcccg ggtctccttt
     2281 taattaaaat tcccaaaaaa acgggtatgg agaaacagta gagagttgcg ataaaaagcg
     2341 tcaggtagga tccgctaatc ttatggataa aaatgctatg gcatagcaaa gtgtgacgcc
     2401 gtgcaaataa tcaatgtgga cttttctgcc gtgattatag acacttttgt tacgcgtttt
     2461 tgtcatggct ttggtcccgc tttgttacag aatgctttta ataagcgggg ttaccggttt
     2521 ggttagcgag aagagccagt aaaagacgca gtgacggcaa tgtctgatgc aatatggaca
     2581 attggtttct tctctgaatg gcgggagtat gaaaagtatg gctgaagcgc aaaatgatcc
     2641 cctgctgccg ggatactcgt ttaatgccca tctggtggcg ggtttaacgc cgattgaggc
     2701 caacggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa
     2761 tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tttgccgacc
     2821 gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc
     2881 tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct
     2941 taactggccg tcaatatttg ccaatacggg gttctttcgc ccggatgaag cgcaccagcc
     3001 gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag ggcgctattc
     3061 ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg aagcgattaa
     3121 cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga
     3181 tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcttgtcgcc
     3241 gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga
     3301 ggaccaacgt atcagccagg cgaagctgct tttgagcacc acccggatgc ctatcgccac
     3361 cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgggtat ttaaaaaatg
     3421 caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga atgatgtagc
     3481 cgtcaagttg tcataataaa tcgactagta ttgagctcca gcttttgttc cctttagtga
     3541 gttagtgtct aggttcccac tagatctcaa atgtgctggg cacatgtttg atttataagg
     3601 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     3661 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     3721 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3781 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3841 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3901 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3961 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4021 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4081 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4141 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4201 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4261 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4321 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4381 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4441 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4501 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4561 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4621 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4681 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4741 tttaaaagga tctaggtgaa gatccttttt gataatctca acatgttgcc aacttactga
     4801 tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagctgtccc
     4861 t
//
