LOCUS       40924_46183        5193 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pWAS-anti-yfiD-anti-purB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced 
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
  PUBMED    30019750
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291, 
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 5193)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5193)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea 
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      292..880
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      1123..1150
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1191..1234
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     ncRNA           1252..1330
                     /product="micC"
                     /label=antisense_RNA
                     /ncRNA_class="antisense_RNA"
     misc_binding    complement(1331..1354)
                     /label=anti-yfiD binding site
                     /bound_moiety="anti-yfiD"
     regulatory      complement(1355..1403)
                     /label=lambda PR promoter
                     /note="lambda PR promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(1423..1439)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(1446..1473)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(1489..1560)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1578..2288)
                     /label=lambda repressor
                     /note="phage lambda repressor"
     promoter        complement(2331..2615)
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             2642..3517
                     /label=araC
                     /note="L-arabinose regulatory protein"
     regulatory      3712..3760
                     /label=lambda PR promoter
                     /note="lambda PR promoter"
                     /regulatory_class="promoter"
     misc_binding    3761..3784
                     /label=anti-purB binding site
                     /bound_moiety="anti-purB"
     ncRNA           3785..3863
                     /product="micC"
                     /label=antisense_RNA
                     /ncRNA_class="antisense_RNA"
     terminator      3878..3949
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3965..3992
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        4050..4154
                     /label=AmpR promoter
     CDS             4155..5012
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 catatgagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
       61 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
      121 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
      181 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
      241 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      301 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      361 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      421 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
      481 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      541 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      601 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      661 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      721 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      781 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      841 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
      901 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      961 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1021 aacgaccgag cgcagcgagt cagtgagcga ggaacatatg tgacagctta tcgcatgcct
     1081 aggtaaaccc aggaggtata gggcgaattg ggtacctata aacgcagaaa ggcccacccg
     1141 aaggtgagcc agtgtgactc tagtagagag cgttcaccga caaacaacag ataaaacgaa
     1201 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggctcga gaaaaaaagc
     1261 ccggacgact gttcgggctt gtctttttat atgttggaaa atcagtggca atgcaatggc
     1321 ccaacagaaa atgattacag gtatccagat tactgcaacc attatcaccg ccagaggtaa
     1381 aatagtcaac acgcacggtg ttagaattcc tgcagcccgg gggatccact agttctagat
     1441 ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga gagcgttcac
     1501 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
     1561 atgcctggga attcttagcc aaacgtctct tcaggccact gactagcgat aacttccccc
     1621 acaacggaac aactctcatt gcatgggatc attgggtact gtgggtttag tggttgtaaa
     1681 aacacctgac cgctatccct gatcagtttc ttgaaggtaa actcatcacc cccaagtctg
     1741 gctatgcaga aatcacctgg ctcaacagcc tgctcagggt caacgagaat taacattccg
     1801 tcaggaaagc ttggcttgga gcctgttggt gcggtcatgg aattaccttc aacctcaagc
     1861 cagaatgcag aatcactggc ttttttggtt gtgcttaccc atctctccgc atcacctttg
     1921 gtaaaggttc taagcttagg tgagaacatc cctgcctgaa catgagaaaa aacagggtac
     1981 tcatactcac ttctaagtga cggctgcata ctaaccgctt catacatctc gtagatttct
     2041 ctggcgattg aagggctaaa ttcttcaacg ctaactttga gaatttttgc aagcaatgcg
     2101 gcgttataag catttaatgc attgatgcca ttaaataaag caccaacgcc tgactgcccc
     2161 atccccatct tgtctgcgac agattcctgg gataagccaa gttcattttt ctttttttca
     2221 taaattgctt taaggcgacg tgcgtcctca agctgctctt gtgttaatgg tttctttttt
     2281 gtgctcatat cccgggtctc cttttaatta aaattcccaa aaaaacgggt atggagaaac
     2341 agtagagagt tgcgataaaa agcgtcaggt aggatccgct aatcttatgg ataaaaatgc
     2401 tatggcatag caaagtgtga cgccgtgcaa ataatcaatg tggacttttc tgccgtgatt
     2461 atagacactt ttgttacgcg tttttgtcat ggctttggtc ccgctttgtt acagaatgct
     2521 tttaataagc ggggttaccg gtttggttag cgagaagagc cagtaaaaga cgcagtgacg
     2581 gcaatgtctg atgcaatatg gacaattggt ttcttctctg aatggcggga gtatgaaaag
     2641 tatggctgaa gcgcaaaatg atcccctgct gccgggatac tcgtttaatg cccatctggt
     2701 ggcgggttta acgccgattg aggccaacgg ttatctcgat ttttttatcg accgaccgct
     2761 gggaatgaaa ggttatattc tcaatctcac cattcgcggt cagggggtgg tgaaaaatca
     2821 gggacgagaa tttgtttgcc gaccgggtga tattttgctg ttcccgccag gagagattca
     2881 tcactacggt cgtcatccgg aggctcgcga atggtatcac cagtgggttt actttcgtcc
     2941 gcgcgcctac tggcatgaat ggcttaactg gccgtcaata tttgccaata cggggttctt
     3001 tcgcccggat gaagcgcacc agccgcattt cagcgacctg tttgggcaaa tcattaacgc
     3061 cgggcaaggg gaagggcgct attcggagct gctggcgata aatctgcttg agcaattgtt
     3121 actgcggcgc atggaagcga ttaacgagtc gctccatcca ccgatggata atcgggtacg
     3181 cgaggcttgt cagtacatca gcgatcacct ggcagacagc aattttgata tcgccagcgt
     3241 cgcacagcat gtttgcttgt cgccgtcgcg tctgtcacat cttttccgcc agcagttagg
     3301 gattagcgtc ttaagctggc gcgaggacca acgtatcagc caggcgaagc tgcttttgag
     3361 caccacccgg atgcctatcg ccaccgtcgg tcgcaatgtt ggttttgacg atcaactcta
     3421 tttctcgcgg gtatttaaaa aatgcaccgg ggccagcccg agcgagttcc gtgccggttg
     3481 tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcataa taaatcgact agtattgagc
     3541 tccagctttt gttcccttta gtgagttagt gtctaggttc ccactagatc tcaaatgtgc
     3601 tgggcacatg tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3661 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgaatt ctaacaccgt
     3721 gcgtgttgac tattttacct ctggcggtga taatggttgc ggcggtcagt gaggataatt
     3781 ccattttctg ttgggccatt gcattgccac tgattttcca acatataaaa agacaagccc
     3841 gaacagtcgt ccgggctttt tttctcgagc caggcatcaa ataaaacgaa aggctcagtc
     3901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     3961 cactggctca ccttcgggtg ggcctttctg cgtttatagg tacccaattc gccgcttaca
     4021 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4081 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4141 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4201 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4261 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4321 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4381 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4441 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4501 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4561 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4621 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4681 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4741 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4801 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4861 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4921 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4981 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5041 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5101 ttgataatct caacatgttg ccaacttact gatttagtgt atgatggtgt ttttgaggtg
     5161 ctccagtggc ttctgtttct atcagctgtc cct
//
