LOCUS       40924_46168       13426 bp DNA     circular SYN 18-DEC-2018
DEFINITION  RNAi cloning vector pWALIUM10, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13426)
  AUTHORS   Ni J-Q., Liu L-P., Perkins LA, Perrimon N.
  TITLE     Vectors for transgenic flies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13426)
  AUTHORS   Ni J-Q., Liu L-P., Perkins LA, Perrimon N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2010) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 13426)
  AUTHORS   Hu Y, Perkins LA.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2012) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   4  (bases 1 to 13426)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 13426)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-FEB-2010) Genetics, Harvard Medical School, 77 Avenue Louis 
            Pasteur, Boston, MA 02115, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (20-JUN-2012) Genetics, Harvard Medical School, 77 Avenue Louis 
            Pasteur, Boston, MA 02115, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Jun 20, 2012 this sequence version replaced GU931388.1.
FEATURES             Location/Qualifiers
     source          1..13426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    682..4808
                     /label=white
                     /note="white"
     CDS             complement(join(1444..2058,2129..2260,2481..2796,2858..3512,
                     3587..3860,4255..4326))
                     /codon_start=1
                     /product="white"
                     /label=white
                     /note="selectable marker"
                     /protein_id="ADE08350.1"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     intron          3513..3586
                     /note="white intron"
     protein_bind    4923..4992
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     protein_bind    5230..5660
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     protein_bind    5678..5711
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5724..5818
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_recomb     5834..5867
                     /label=LoxP
                     /note="LoxP"
     protein_bind    complement(5834..5867)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    5874..5983
                     /label=5XUAS
                     /note="5XUAS"
     protein_bind    5880..5974
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5998..6236
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    6281..6405
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6430..6460
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6514..7170
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             7515..7817
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(7861..7985)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          8008..8154
                     /note="ftz intron"
     protein_bind    8165..8289
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(8333..8635)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(8980..9636)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(9690..9720)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(9745..9869)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          9916..10062
                     /note="ftz intron"
     intron          10162..10227
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10357..10377
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    10649..10783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    10792..11222
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     promoter        complement(11241..11259)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11280..11296)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11304..11320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11328..11358)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11373..11394)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(11682..12270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12444..13301)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(13302..13406)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacaag
      661 cttcacgacc tgaggcgcgc ctccagtgaa atccaagcat tttctaaatt aaatgtattc
      721 ttattattat agttgttatt tttgatatat ataaacaaca ctattatgcc caccattttt
      781 ttgagatgca tctacacaag gaacaaacac tggatgtcac tttcagttca aattgtaacg
      841 ctaatcactc cgaacaggtc acaaaaaatt accttaaaaa gtcataatat taaattagaa
      901 taaatatagc tgtgagggaa atatatacaa atatattgga gcaaataaat tgtacataca
      961 aatatttatt actaatttct attgagacga aatgaaccac tcggaaccat ttgagcgaac
     1021 cgaatcgcgc ggaactaacg acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt
     1081 gcggagagcg ggtgggagac agcgaaagag caactacgaa acgtggtgtg gtggaggtga
     1141 attatgaaga gggcgcgcga tttgaaaagt atgtatataa aaaatatatc ccggtgtttt
     1201 atgtagcgat aaacgagttt ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga
     1261 agacaaatcc aaagtctact tgtggggatg ttcgaagggg aaatacttgt attctatagg
     1321 tcatatcttg tttttattgg cacaaatata attacattag ctttttgagg gggcaataaa
     1381 cagtaaacac gatggtaata atggtaaaaa aaaaaacaag cagttatttc ggatatatgt
     1441 cggctactcc ttgcgtcggg cccgaagtct tagagccaga tatgcgagca cccggaagct
     1501 cacgatgaga atggccagac ccacgtagtc cagcggcaga tcggcggcgg agaagttaag
     1561 cgtctccagg atgaccttgc ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat
     1621 ttcgcccggc tccacgtccg cccattggtt aatcagcaga ccctcgttgg cgtaacggaa
     1681 ccatgagagg tacgacaacc atttgaggta tactggcacc gagcccgagt tcaagaagaa
     1741 gccgccaaag agcaggaatg gtatgataac cggcggaccc acagacagcg ccatcgaggt
     1801 cgaggagctg gcgcaggata ttagatatcc gaaggacgtt gacacattgg ccaccagagt
     1861 gaccagcgcc aggcagttga agaagtgcag cactccggcc cgcagtccga tcatcggata
     1921 ggcaatcgcc gtgaagacca gtggcactgt gagaaaaagc ggtaattcgg caatcgtttt
     1981 gcccagaaag tatgtgtcac agcgataaag tcgacttcgg gcctccctca taaaaactgg
     2041 cagctctgag gtgaacacct aaatcgaatc gattcattag aaagttagta aattattaat
     2101 atgcaaatgt attctaaaca agacttacat ttatcgtggc aaagacgttt tgaaaggtca
     2161 tgttggtcag gaagaggaag atggctccgt tgatattcat cacgcccact tgcgtgagtt
     2221 gttggcccaa aaagatgagg ccaatcaaga tggcaaccat ctgcaaatta aaatgttact
     2281 cgcatctcat taatattcat atcttcaaca tgttcgcgag ttaaatgaaa tttatttatt
     2341 ttctgcaaaa ctataaacta tacatctcat tgaaaaaaac taagaagggt gtggaatcag
     2401 gcaattctaa ctaaaatcta gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg
     2461 tttccactgg aaccactcac cgttgtctga ataagtcgca cttttacgag gagtggttcc
     2521 ttgagcaccg acagccagga tcgccacagg accgcccgga actgcatgaa ccaggtggcc
     2581 ttgtaggtgt acccattctc cggctgctcc agtggcttct ccaaattttt ggtggccaac
     2641 aactgctcca tatcccgggc tactttgcta atagcaaaat tgtcgcatat cttggcgatc
     2701 cgatcacggg actcgatctc ccgtccgggc acaacggcca acacctgtac gtaaaagtcc
     2761 gccggattgt agttggtagg acactgggca cccacgctgg ataggagttg agatgtaatg
     2821 taatgctaga tacccttaat aaacacatcg aactcactag gaaaagaagt cgacggcttc
     2881 gctgggagtg cccaagaaag ctaccctgcc ctcggccatc agaaggatct tgtcaaagag
     2941 ctcaaacagc tcggaagacg gctgatgaat ggtcaggatg acggtcttgc ccttctgcga
     3001 cagcttcttc agcacctgga cgacgctgtg ggcggtaaat gagtccagtc cggaggtggg
     3061 ctcatcgcag atcagaagcg gcggatcggt tagtgcctcg gaggcgaatg ccagacgctt
     3121 cctttctccg ccggacagac ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca
     3181 tttgctgagc gaaagctcct ggatcacctg atccacgcgg gccactcgct gccgataggt
     3241 cagatgtcgt ggcatccgca ccatggcctg gaaaatcagg tgttccctgg ccgttaggga
     3301 gccgataaag aggtcatcct gctggacata ggcgcacctg gcctgcatct ccttggcgtc
     3361 cacaggttgg ccattgagca gtcgcatccc ggatggcgat acttggatgc cctgcggcga
     3421 tcgaaaggca agggcattca gcagggtcgt ctttccggca ccggaactgc ccatcacggc
     3481 caaaagttcg cccggatagg ccacgccgca aactgagttt caaattggta attggaccct
     3541 ttattaagat ttcacacaga tcagccgact gcgaatagaa actcaccgtt cttgagcaaa
     3601 tgtttcctgg gcgccggtat gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc
     3661 agctgccgcc atccggagcc cggctgattg accgccccaa agatgtccat attgtgccag
     3721 gcataggtga ggttctcggc tagttggccg ctccctgaac cggagtcctc cggcggactg
     3781 ggtggcagga gcgtgccgta gtttttggcc tgcccgaagc cctggttaat gcagctctgc
     3841 gaagccgctc cgctgtcacc ctgcaatgat aggggatctc aaatatcaac tacaagcgtt
     3901 atgctcatct aaccccgaac aaaacgaagt atcctacgaa gtaggtttat acttttattt
     3961 attttttgtg catctaggat cagcttaaaa tatctggttg ttatattttt tgtaaaaaag
     4021 aatgtagtcg aaaatgaatg cctttagatg tcttgatcat gatatgatct taaaaattgt
     4081 cttatatagc gagcacagct accagaataa tctgtttcgt gtcactattt gtttgtgcga
     4141 ttgcggtttg ggatttttgt gggtcgcagt tctcacgccg cagacaattt gatgttgcaa
     4201 tcgcagttcc tatagatcaa gtgaacttaa gatgtatgca catgtactac tcacattgtt
     4261 cagatgctcg gcagatgggt gtttgctgcc tccgcgaatt aatagctcct gatcctcttg
     4321 gcccattgcc gggatttttc acactttccc ctgcttaccc acccaaaacc aatcaccacc
     4381 ccaatcactc aaaaaacaaa caaaaataag aagcgagagg agttttggca cagcactttg
     4441 tgtttaattg atggcgtaaa ccgcttggag cttcgtcacg aaaccgctga caaagtgcaa
     4501 ctgaaggcgg acattgacgc taggtaacgc tacaaacggt ggcgaaagag atagcggacg
     4561 cagcggcgaa agagacggcg atatttctgt ggacagagaa ggaggcaaac agcgctgact
     4621 ttgagtggaa tgtcattttg agtgagaggt aatcgaaaga acctggtact tcaaataccc
     4681 ttggatcgaa gtaaatttaa aactgatcag ataagttcaa tgatatccag tgcagtaaaa
     4741 aataaaaaaa aaaaatgttt ttttttatct actttccgca aaaatgggtt ttattaactt
     4801 acatacatgg cgcgccagat cgcaagaagc ttgatatcat cgatctcgag gctgcatcca
     4861 acgcgttggg agctctccgg atcaattcgg cttcaggtac cgtcgacgat gtaggtcacg
     4921 gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc
     4981 acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt gatcccggcg
     5041 aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg
     5101 agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc
     5161 tcgacggtca cggcgggcat gtcgacaagc cgaattgatc cactagaagg cctaattcgg
     5221 tacactagtt ggccacgtaa taagtgtgcg ttgaatttat tcgcaaaaac attgcatatt
     5281 ttcggcaaag taaaattttg ttgcatacct tatcaaaaaa taagtgctgc atacttttta
     5341 gagaaaccaa ataatttttt attgcatacc cgtttttaat aaaatacatt gcataccctc
     5401 ttttaataaa aaatattgca tactttgacg aaacaaattt tcgttgcata cccaataaaa
     5461 gattattata ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa
     5521 aatattgcat acgttgacga aacaaatttt cgttgcatac ccaataaaag attattatat
     5581 tgcatacctt ttcttgccat accatttagc cgatcaattg tgctcggcaa cagtatattt
     5641 gtggtgtgcc aaccaacaac actagtagta ccagcttata acttcgtata atgtatgcta
     5701 tacgaagtta tctgcaggca ggtcggagta ctgtcctccg agcggagtac tgtcctccga
     5761 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
     5821 gagactccca tggataactt cgtataatgt atgctatacg aagttatgga tccgcaggtc
     5881 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     5941 gtactgtcct ccgagcggag tactgtcctc cgagcggaga ctcgtcgaca gcgagcgccg
     6001 gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga
     6061 acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc
     6121 tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa
     6181 ctgcaactac tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata
     6241 gggaattggg aattccagat ctgcggccgc ggctcgaatc acaagtttgt acaaaaaagc
     6301 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     6361 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
     6421 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
     6481 ccggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     6541 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     6601 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     6661 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     6721 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     6781 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     6841 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     6901 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     6961 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     7021 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     7081 caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     7141 cagtactgcg atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc
     7201 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     7261 tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca gtgacagttg
     7321 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     7381 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     7441 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     7501 cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     7561 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     7621 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     7681 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     7741 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     7801 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     7861 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     7921 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
     7981 gtggtgattc gagggtacct ctagaaggta ggcatcacac acgattaaca acccctaaaa
     8041 atacactttg aaaatattga aaatatgttt ttgtatacat ttttgatatt ttcaaacaat
     8101 acgcagttat aaaactcatt agctaaccca ttttttcttt gcttatgctt acaggtcgac
     8161 tgtgaccact ttgtacaaga aagctgaacg agaaacgtaa aatgatataa atatcaatat
     8221 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     8281 gtcactatgg tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc
     8341 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
     8401 cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
     8461 catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg
     8521 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
     8581 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
     8641 cagtccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
     8701 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
     8761 atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc
     8821 tgtcgctgtc aactgtcact gtaatacgct gcttcatagc acacctcttt ttgacatact
     8881 tcgggtatac atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata
     8941 ctgttatctg gcttttagta agccggatcc acgcgtttac gccccgccct gccactcatc
     9001 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac agacggcatg
     9061 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     9121 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     9181 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     9241 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     9301 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     9361 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa
     9421 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     9481 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     9541 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     9601 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     9661 tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa
     9721 gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat
     9781 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     9841 tctcgttcag cttttttgta caaacttgtt ctagagcaaa ctagttctga tctgctagac
     9901 aattgttggc atcaggtagg catcacacac gattaacaac ccctaaaaat acactttgaa
     9961 aatattgaaa atatgttttt gtatacattt ttgatatttt caaacaatac gcagttataa
    10021 aactcattag ctaacccatt ttttctttgc ttatgcttac agattgcaaa gaactagagc
    10081 cgcgggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
    10141 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
    10201 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
    10261 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
    10321 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
    10381 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
    10441 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
    10501 aaaattatgg aaaaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
    10561 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    10621 acataaaatg aatggaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    10681 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10741 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ttccagagct ctggccacgt
    10801 aataagtgtg cgttgaattt attcgcaaaa acattgcata ttttcggcaa agtaaaattt
    10861 tgttgcatac cttatcaaaa aataagtgct gcatactttt tagagaaacc aaataatttt
    10921 ttattgcata cccgttttta ataaaataca ttgcataccc tcttttaata aaaaatattg
    10981 catactttga cgaaacaaat tttcgttgca tacccaataa aagattatta tattgcatac
    11041 ccgtttttaa taaaatacat tgcataccct cttttaataa aaaatattgc atacgttgac
    11101 gaaacaaatt ttcgttgcat acccaataaa agattattat attgcatacc ttttcttgcc
    11161 ataccattta gccgatcaat tgtgctcggc aacagtatat ttgtggtgtg ccaaccaaca
    11221 acgagctcca gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg
    11281 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    11341 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    11401 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    11461 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
    11521 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    11581 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    11641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    11701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    11761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    11821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    11881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    11941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    12001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    12061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    12121 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    12181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    12241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    12301 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    12361 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    12421 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    12481 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    12541 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    12601 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    12661 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    12721 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    12781 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    12841 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    12901 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    12961 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    13021 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    13081 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    13141 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    13201 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    13261 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    13321 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    13381 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgc
//
