LOCUS       40924_45993        6125 bp DNA     circular SYN 18-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        9..312
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          700..1644
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     polyA_signal    complement(1728..1849)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2037..2625
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        2747..2938
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     intron          3145..4092
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     misc_feature    4167..4611
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the
                     foot-and-mouth disease virus"
     promoter        4644..4691
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             4711..5502
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5552..6124
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcaggcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
       61 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      121 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      181 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      241 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      301 gctattacca tgatgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      361 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttgactagtc
      421 agggccccaa cccccccaag cccccatttc acaacacgct ggcgctacag gcgcgtgact
      481 tccccttgct ttggggcggg gggctgagac tcctatgtgc tccggattgg tcaggcacgg
      541 ccttcggccc cgcctcctgc caccgcagat tggccgctag gcctccccga gcgccctgcc
      601 tccgagggcc ggcgcaccat aaaagaagcc gccctagcca cgtcccctcg cagttcggcg
      661 gtcccgcggg tctgtctcaa gcttgccgcc agaacacagg taagtgccgt gtgtggttcc
      721 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccatgccc
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcttgggcg
      901 ctggggccgc cgcgtgctaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgctaa
      961 gtctctagcc atttaaaatt tttgataacc agctgcgacg ctttttttct ggcgagatag
     1021 tcttgtaaat gcgggccagg atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1141 accgagaatc ggacgggggt agtctcaaac tggccggcct gctctggtgc ctggcctcgc
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1321 cccgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cttcctcatc
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttgtc
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1501 ccacactgag tgggtggaga ctgaagagtt aggccagctt ggcacttgat gtaattctcc
     1561 ttggaatttg ccctttttga gtttggatct tgcctcattc tcaagcctca gacagtggtt
     1621 caaagttttt ttcttccatt tcaggtgtcg tgaaaactac ccctaaaagc caccggcgtg
     1681 cgcaagatct gaattcttcg aactcgaggc tagctggcca gacatgataa gatacattga
     1741 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     1801 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     1861 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     1921 aaacctctac aaatgtggta tggaaatgtt aattaactag ccatgaccaa aatcccttaa
     1981 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2041 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2101 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2161 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2221 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2281 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2341 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2401 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2461 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2521 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2581 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2641 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt aattaacctg
     2701 cagggcctga aataacctct gaaagaggaa cttggttagg taccttctga ggctgaaaga
     2761 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     2821 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     2881 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccac
     2941 tagttccgcc agagcgcgcg agggcctcca gcggccgccc ctcccccaca gcaggggcgg
     3001 ggtcccgcgc ccaccggaag gagcgggctc ggggcgggcg gcgctgattg gccggggcgg
     3061 gcctgacgcc gacgcggcta taagagacca caagcgaccc gcagggccag acgttcttcg
     3121 ccgaagcttg ccgtcagaac gcaggtgagg ggcgggtgtg gcttccgcgg gccgccgagc
     3181 tggaggtcct gctccgagcg ggccgggccc cgctgtcgtc ggcggggatt agctgcgagc
     3241 attcccgctt cgagttgcgg gcggcgcggg aggcagagtg cgaggcctag cggcaacccc
     3301 gtagcctcgc ctcgtgtccg gcttgaggcc tagcgtggtg tccgcgccgc cgccgcgtgc
     3361 tactccggcc gcactctggt cttttttttt tttgttgttg ttgccctgct gccttcgatt
     3421 gccgttcagc aataggggct aacaaaggga gggtgcgggg cttgctcgcc cggagcccgg
     3481 agaggtcatg gttggggagg aatggaggga caggagtggc ggctggggcc cgcccgcctt
     3541 cggagcacat gtccgacgcc acctggatgg ggcgaggcct ggggtttttc ccgaagcaac
     3601 caggctgggg ttagcgtgcc gaggccatgt ggccccagca cccggcacga tctggcttgg
     3661 cggcgccgcg ttgccctgcc tccctaacta gggtgaggcc atcccgtccg gcaccagttg
     3721 cgtgcgtgga aagatggccg ctcccgggcc ctgttgcaag gagctcaaaa tggaggacgc
     3781 ggcagcccgg tggagcgggc gggtgagtca cccacacaaa ggaagagggc ctggtccctc
     3841 accggctgct gcttcctgtg accccgtggt cctatcggcc gcaatagtca cctcgggctt
     3901 ttgagcacgg ctagtcgcgg cggggggagg ggatgtaatg gcgttggagt ttgttcacat
     3961 ttggtgggtg gagactagtc aggccagcct ggcgctggaa gtcatttttg gaatttgtcc
     4021 ccttgagttt tgagcggagc taattctcgg gcttcttagc ggttcaaagg tatcttttaa
     4081 accctttttt aggtgttgtg aaaaccaccg ctaattcaaa gcaaccggtg atatcggatc
     4141 cacgcgtatc gattgtcgac cctaggagca ggtttcccca atgacacaaa acgtgcaact
     4201 tgaaactccg cctggtcttt ccaggtctag aggggtaaca ctttgtactg cgtttggctc
     4261 cacgctcgat ccactggcga gtgttagtaa cagcactgtt gcttcgtagc ggagcatgac
     4321 ggccgtggga actcctcctt ggtaacaagg acccacgggg ccaaaagcca cgcccacacg
     4381 ggcccgtcat gtgtgcaacc ccagcacggc gactttactg cgaaacccac tttaaagtga
     4441 cattgaaact ggtacccaca cactggtgac aggctaagga tgcccttcag gtaccccgag
     4501 gtaacacgcg acactcggga tctgagaagg ggactggggc ttctataaaa gcgctcggtt
     4561 taaaaagctt ctatgcctga ataggtgacc ggaggtcggc acctttcctt tgcaattact
     4621 gaccctatga atacaactga ctgtttgaca attaatcatc ggcatagtat atcggcatag
     4681 tataatacga ctcactatag gagggccacc atgattgaac aagatggatt gcacgcaggt
     4741 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     4801 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     4861 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     4921 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     4981 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     5041 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     5101 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     5161 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     5221 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac acatggcgat
     5281 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     5341 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     5401 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5461 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     5521 tcgaaatgac cgaccaagcg aattcgctag gattatccct aatacctgcc accccactct
     5581 taatcagtgg tggaagaacg gtctcagaac tgtttgtttc aattggccat ttaagtttag
     5641 tagtaaaaga ctggttaatg ataacaatgc atcgtaaaac cttcagaagg aaaggagaat
     5701 gttttgtgga ccactttggt tttctttttt gcgtgtggca gttttaagtt attagttttt
     5761 aaaatcagta ctttttaatg gaaacaactt gaccaaaaat ttgtcacaga attttgagac
     5821 ccattaaaaa agttaaatga gaaacctgtg tgttcctttg gtcaacaccg agacatttag
     5881 gtgaaagaca tctaattctg gttttacgaa tctggaaact tcttgaaaat gtaattcttg
     5941 agttaacact tctgggtgga gaatagggtt gttttccccc cacataattg gaaggggaag
     6001 gaatatcatt taaagctatg ggagggttgc tttgattaca acactggaga gaaatgcagc
     6061 atgttgctga ttgcctgtca ctaaaacagg ccaaaaactg agtccttggg ttgcatagaa
     6121 agctg
//
