LOCUS       V012768                 8803 bp    DNA     circular SYN 18-SEP-2021
DEFINITION  Exported.
ACCESSION   V012768
VERSION     V012768
KEYWORDS    pVgRXR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8803)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8803)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..359
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        789..836
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             856..1227
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    1294..1428
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1592..2180)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     enhancer        2265..2568
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2569..2772
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        2907..2925
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3038..3271
                     /label="VP16 AD"
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et
                     al., 1989)"
     polyA_signal    5535..5583
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        6352..6613
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             6637..8022
                     /gene="RXRA"
                     /label="Retinoic acid receptor RXR-alpha"
                     /note="Retinoic acid receptor RXR-alpha from Homo sapiens.
                     Accession#: P19793"
     promoter        complement(8064..8082)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    8108..8332
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8378..8803
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 attatggtga ccggatccgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
       61 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      181 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      241 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      301 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      361 agctggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
      421 ggtgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      481 ggtaggctcc aagggagcgc cggacaaagg cccggtctcg acctgagctc taaacttacc
      541 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      601 ttcgttttct gttctgcgcc gttacaactt caaggtatgc gctgggacct ggcaggcggc
      661 atctgggacc cctaggaagg gcttgggggt cctcgtgccc aaggcaggga acatagtggt
      721 cccaggaagg ggagcagagg catcagggtg tccactttgt ctccgcagct cctgagcctg
      781 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgactcac
      841 tataggaggg ccaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
      901 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
      961 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     1021 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     1081 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     1141 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     1201 gtgcacttcg tggccgagga gcaggactga ccgacgccga ccaacaccgc cggtccgacg
     1261 cggcccgacg ggtccgaggg gggtcgacct cgaaacttgt ttattgcagc ttataatggt
     1321 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1381 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccc tcggagatcg
     1441 atctgggccc atgcggccgg ccgcggatcg atgctcactc aaaggcggta atacggttat
     1501 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1561 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1621 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1681 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1741 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1801 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1861 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1921 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1981 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2041 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2101 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2161 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2221 ggaacgaaaa ctcacgttaa gggattttgg tcatggctag agtccgttac ataacttacg
     2281 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2341 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     2401 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     2461 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     2521 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     2581 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     2641 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     2701 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     2761 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2821 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
     2881 acggacctgc agcacgtgtt gacaattaat acgactcact atagggagac ccaagcttgg
     2941 taccagatct tttaaagcta gctctagagt accgagctcg gatcccccgg gccagtgtga
     3001 tggatatctg cagaattcag ggtttaaagc caccatggcc cccccgaccg atgtcagcct
     3061 gggggacgaa ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga
     3121 cgatttcgat ctggacatgt tgggggacgg ggattcccca ggtccgggat ttacccccca
     3181 cgactccgcc ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac
     3241 cgatgccctt ggaattgacg agtacggtgg gaagcttcta ggtacctcta gaagaatatc
     3301 aaattctata tcttcaggtc gcgatgatct ctcgccttcg agcagcttga acggatactc
     3361 ggcgaacgaa agctgcgatg cgaagaagag caagaaggga cctgcgccac gggtgcaaga
     3421 ggagctgtgc ctggtttgcg gcgacagggc ctccggctac cactacaacg ccctcacctg
     3481 tggatcctgc aaggtgttct ttcgacgcag cgttacgaag agcgccgtct actgctgcaa
     3541 gttcgggcgc gcctgcgaaa tggacatgta catgaggcga aagtgtcagg agtgccgcct
     3601 gaaaaagtgc ctggccgtgg gtatgcggcc ggaatgcgtc gtcccggaga accaatgtgc
     3661 gatgaagcgg cgcgaagaga aggcccagaa ggagaaggac aaaatgacca cttcgccgag
     3721 ctctcagcat ggcggcaatg gcagcttggc ctctggtggc ggccaagact ttgttaagaa
     3781 ggagattctt gaccttatga catgcgagcc gccccagcat gccactattc cgctactacc
     3841 tgatgaaata ttggccaagt gtcaagcgcg caatatacct tccttaacgt acaatcagtt
     3901 ggccgttata tacaagttaa tttggtacca ggatggctat gagcagccat ctgaagagga
     3961 tctcaggcgt ataatgagtc aacccgatga gaacgagagc caaacggacg tcagctttcg
     4021 gcatataacc gagataacca tactcacggt ccagttgatt gttgagtttg ctaaaggtct
     4081 accagcgttt acaaagatac cccaggagga ccagatcacg ttactaaagg cctgctcgtc
     4141 ggaggtgatg atgctgcgta tggcacgacg ctatgaccac agctcggact caatattctt
     4201 cgcgaataat agatcatata cgcgggattc ttacaaaatg gccggaatgg ctgataacat
     4261 tgaagacctg ctgcatttct gccgccaaat gttctcgatg aaggtggaca acgtcgaata
     4321 cgcgcttctc actgccattg tgatcttctc ggaccggccg ggcctggaga aggcccagct
     4381 agtcgaagcg atccagagct actacatcga cacgctacgc atttatatac tcaaccgcca
     4441 ctgcggcgac tcaatgagcc tcgtcttcta cgcaaagctg ctctcgatcc tcaccgagct
     4501 gcgtacgctg ggcaaccaga acgccgagat gtgtttctca ctaaagctca aaaaccgcaa
     4561 actgcccaag ttcctcgagg agatctggga cgttcatgcc atcccgccat cggtccagtc
     4621 gcaccttcag attacccagg aggagaacga gcgtctcgag cgggctgagc gtatgcgggc
     4681 atcggttggg ggcgccatta ccgccggcat tgattgcgac tctgcctcca cttcggcggc
     4741 ggcagccgcg gcccagcatc agcctcagcc tcagccccag ccccaaccct cctccctgac
     4801 ccagaacgat tcccagcacc agacacagcc gcagctacaa cctcagctac cacctcagct
     4861 gcaaggtcaa ctgcaacccc agctccaacc acagcttcag acgcaactcc agccacagat
     4921 tcaaccacag ccacagctcc ttcccgtctc cgctcccgtg cccgcctccg taaccgcacc
     4981 tggttccttg tccgcggtca gtacgagcag cgaatacatg ggcggaagtg cggccatagg
     5041 accaatcacg ccggcaacca ccagcagtat cacggctgcc gttaccgcta gctccaccac
     5101 atcagcggta ccgatgggca acggagttgg agtcggtgtt ggggtgggcg gcaacgtcag
     5161 catgtatgcg aacgcccaga cggcgatggc cttgatgggt gtagccctgc attcgcacca
     5221 agagcagctt atcgggggag tggcggttaa gtcggagcac tcgacgactg catagccagt
     5281 gtgctggaag taccgagctc gatcgtaccc agcttggagg tgcagggtcg atgcgacgca
     5341 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     5401 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     5461 cgtccggatc gggagatggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     5521 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg
     5581 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
     5641 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
     5701 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc actggccccg
     5761 tgggttaggg acggggtccc catggggaat ggtttatggt tcgtgggggt tattattttg
     5821 ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga cagacccatg gtttttggat
     5881 ggcctgggca tggaccgcat gtactggcgc gacacgaaca ccgggcgtct gtggctgcca
     5941 aacacccccg acccccaaaa accaccgcgc ggatttctgg cgtgccaagc tagtcgatcg
     6001 accaattctc atgtttgaca gcttatcatc gcagatccgt atggtgcact ctcagtacaa
     6061 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     6121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     6181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     6241 cgcgtatctg aggggactag ggtgtgttta ggcgaaaagc ggggcttcgg ttgtacgcgg
     6301 ttaggagtcc cctcaggata tagtagtttc gcttttgcat agggaggggg aaatgtagtc
     6361 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     6421 aggagagaaa aagcaccgtg catgccgata ggtggaagta aggtggtacg atcgtgcctt
     6481 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tccgcattgc
     6541 agagatattg tatttaagtg cctagctcga tacaataaac gccatttgac cattcaccac
     6601 attggagtgc acctccaagc tgggtacgat atcaccatgg acaccaaaca tttcctgccg
     6661 ctcgatttct ccacccaggt gaactcctcc ctcacctccc cgacggggcg aggctccatg
     6721 gctgccccct cgctgcaccc gtccctgggg cctggcatcg gctccccggg acagctgcat
     6781 tctcccatca gcaccctgag ctcccccatc aacggcatgg gcccgccttt ctcggtcatc
     6841 agctccccca tgggccccca ctccatgtcg gtgcccacca cacccaccct gggcttcagc
     6901 actggcagcc cccagctcag ctcacctatg aaccccgtca gcagcagcga ggacatcaag
     6961 ccccccctgg gcctcaatgg cgtcctcaag gtccccgccc acccctcagg aaacatggct
     7021 tccttcacca agcacatctg cgccatctgc ggggaccgct cctcaggcaa gcactatgga
     7081 gtgtacagct gcgaggggtg caagggcttc ttcaagcgga cggtgcgcaa ggacctgacc
     7141 tacacctgcc gcgacaacaa ggactgcctg attgacaagc ggcagcggaa ccggtgccag
     7201 tactgccgct accagatgtg cctggccatg ggcatgaagc gggaagccgt gcaggaggag
     7261 cggcagcgtg gcaaggaccg gaacgagaat gaggtggagt cgaccagcag cgccaacgag
     7321 gacgtgccgg tggagaggat cctggaggct gagctggccg tggagcccaa gaccgagacc
     7381 tacgtggagg caaacgtggg gctgaacccc agctcgccga acgaccctgt caccaacatt
     7441 tgccaagcag ccgacaaaca gcttttcacc ctggtggagt gggccaagcg gatcccacac
     7501 ttctcagagc tgcccctgga cgaccaggtc atcctgctgc gggcaggctg gaatgagctg
     7561 ctcatcgcct ccttctccca ccgctccatc gccgtgaagg acgggatcct cctggccacc
     7621 gggctgcacg tccaccggaa cagcgcccac agcgcagggg tgggcgccat ctttgacagg
     7681 gtgctgacgg agcttgtgtc caagatgcgg gacatgcaga tggacaagac ggagctgggc
     7741 tgcctgcgcg ccatcgtcct ctttaaccct gactccaagg ggctctcgaa cccggccgag
     7801 gtggaggcgc tgagggagaa ggtctatgcg tccttggagg cctactgcaa gcacaagtac
     7861 ccagagcagc cgggaaggtt cgctaagctc ttgctccgcc tgccggctct gcgctccatc
     7921 gggctcaaat gcctggaaca tctcttcttc ttcaagctca tcggggacac acccattgac
     7981 accttcctta tggagatgct ggaggcgccg caccaaatga cttaggcggc cgctcgagca
     8041 tgcatctagc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca
     8101 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     8161 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     8221 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     8281 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     8341 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     8401 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8461 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8521 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8581 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8641 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8701 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8761 tattggttaa aaaatgagct gatttaacaa aaatttaacg cga
//
