LOCUS       40924_46148        9231 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVZ326, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Obando ST, Babykin MM, Zinchenko VV.
  TITLE     A Cluster of Five Genes Essential for the Utilization of 
            Dihydroxamate Xenosiderophores in Synechocystis sp. PCC 6803
  JOURNAL   Curr. Microbiol. (2018) In press
  PUBMED    29785634
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   Obando TSA., Babykin MM, Zinchenko VV.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2017) Dept. of Genetics, Moscow State University, 
            Moscow 119991, Russian Federation
REFERENCE   3  (bases 1 to 9231)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9231)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. 
            Microbiol. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-OCT-2017) Dept. of Genetics, Moscow State University, Moscow 
            119991, Russian Federation"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(425..1237)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_difference 1239..1240
                     /replace="ac"
                     /compare="AF100176.1"
                     /note="creates NdeI site overlapping the ATG start codon of
                     aphA gene"
     rep_origin      complement(2095..2489)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(2516..2800)
                     /codon_start=1
                     /gene="mobC"
                     /product="mobilization protein C"
                     /label=mobC
                     /protein_id="AWZ62436.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     gene            complement(2516..2800)
                     /gene="mobC"
                     /label=mobC
     oriT            2831..2918
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             3747..4160
                     /codon_start=1
                     /gene="mobB"
                     /product="mobilization protein B"
                     /label=mobB
                     /protein_id="AWZ62438.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     gene            3747..4160
                     /gene="mobB"
                     /label=mobB
     CDS             4157..5125
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             5189..5401
                     /codon_start=1
                     /product="protein E"
                     /label=protein E
                     /protein_id="AWZ62440.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     CDS             5403..5609
                     /codon_start=1
                     /gene="cac"
                     /product="repressor protein F"
                     /label=cac
                     /protein_id="AWZ62441.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     gene            5403..5609
                     /gene="cac"
                     /label=cac
     CDS             5639..6475
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             6465..7313
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     promoter        complement(8064..8168)
                     /label=AmpR promoter
     promoter        8371..8473
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             8474..9130
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 gatctgtaat ccgggcagcg caacggaaca ttcatcagtg taaaaatgga atcaataaag
       61 ccctgcgcag cgcgcagggt cagcctgaat acgcgtttaa tgaccagcac agtcgtgatg
      121 gcaaggtcag aatagcgctg aggtctgcct cgtgaagaag gtgttgctga ctcataccag
      181 gcctgaatcg ccccatcatc cagccagaaa gtgagggagc cacggttgat gagagctttg
      241 ttgtaggtgg accagttggt gattttgaac ttttgctttg ccacggaacg gtctgcgttg
      301 tcgggaagat gcgtgatctg atccttcaac tcagcaaaag ttcgatttat tcaacaaagc
      361 cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta caaccaatta accaattctg
      421 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
      481 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
      541 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
      601 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
      661 ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc
      721 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
      781 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
      841 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
      901 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
      961 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     1021 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     1081 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
     1141 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     1201 gcctcgagca agacgtttcc cgttgaatat ggctcatatg accccttgta ttactgttta
     1261 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     1321 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
     1381 atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa
     1441 tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc
     1501 tggatgatgg ggcgattcag gcctggtatg agtcagcaac accttcttca cgaggcagac
     1561 ctcagcgcta ttctgacctt gccatcacga ctgtgctggt cattaaacgc gtattcaggc
     1621 tgaccctgcg cgctgcgcag ggctttattg attccatttt tacactgatg aatgttccgt
     1681 tgcgctgccc ggattacagc tgaaagcgac caggtgctcg gcgtggcaag actcgcagcg
     1741 aacccgtaga aagccatgct ccagccgccc gcattggaga aattcttcaa attcccgttg
     1801 cacatagccc ggcaattcct ttccctgctc tgccataagc gcagcgaatg ccgggtaata
     1861 ctcgtcaacg atctgataga gaagggtttg ctcgggtcgg tggctctggt aacgaccagt
     1921 atcccgatcc cggctggccg tcctggccgc cacatgaggc atgttccgcg tccttgcaat
     1981 actgtgttta catacagtct atcgcttagc ggaaagttct tttaccctca gccgaaatgc
     2041 ctgccgttgc tagacattgc cagccagtgc ccgtcactcc cgtactaact gtcacgaacc
     2101 cctgcaataa ctgtcacgcc cccctgcaat aactgtcacg aacccctgca ataactgtca
     2161 cgcccccaaa cctgcaaacc cagcaggggc gggggctggc ggggtgttgg aaaaatccat
     2221 ccatgattat ctaagaataa tccactaggc gcggttatca gcgcccttgt ggggcgctgc
     2281 tgcccttgcc caatatgccc ggccagaggc cggatagctg gtctattcgc tgcgctaggc
     2341 tacacaccgc cccaccgctg cgcggcaggg ggaaaggcgg gcaaagcccg ctaaacccca
     2401 caccaaaccc cgcagaaata cgctggagcg cttttagccg ctttagcggc ctttccccct
     2461 acccgaaggg tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg atcattcagc
     2521 ccggctcatc cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg tcgcgttcaa
     2581 ggtacgcatc cattgccgcc atgagccgat cctccggcca ctcgctgctg ttcaccttgg
     2641 ccaaaatcat ggcccccacc agcaccttgc gccttgtttc gttcttgcgc tcttgctgct
     2701 gttcccttgc ccgcacccgc tgaatttcgg cattgattcg cgctcgttgt tcttcgagct
     2761 tggccagccg atccgccgcc ttgttgctcc ccttaaccat cttgacaccc cattgttaat
     2821 gtgctgtctc gtaggctatc atggaggcac agcggcggca atcccgaccc tactttgtag
     2881 gggagggcgc acttaccggt ttctcttcga gaaactggcc taacggccac ccttcgggcg
     2941 gtgcgctctc cgagggccat tgcatggagc cgaaaagcaa aagcaacagc gaggcagcat
     3001 ggcgatttat caccttacgg cgaaaaccgg cagcaggtcg ggcggccaat cggccagggc
     3061 caaggccgac tacatccagc gcgaaggcaa gtatgcccgc gacatggatg aagtcttgca
     3121 cgccgaatcc gggcacatgc cggagttcgt cgagcggccc gccgactact gggatgctgc
     3181 cgacctgtat gaacgcgcca atgggcggct gttcaaggag gtcgaatttg ccctgccggt
     3241 cgagctgacc ctcgaccagc agaaggcgct ggcgtccgag ttcgcccagc acctgaccgg
     3301 tgccgagcgc ctgccgtata cgctggccat ccatgccggt ggcggcgaga acccgcactg
     3361 ccacctgatg atctccgagc ggatcaatga cggcatcgag cggcccgccg ctcagtggtt
     3421 caagcggtac aacggcaaga ccccggagaa gggcggggca cagaagaccg aagcgctcaa
     3481 gcccaaggca tggcttgagc agacccgcga ggcatgggcc gaccatgcca accgggcatt
     3541 agagcgggct ggccacgacg cccgcattga ccacagaaca cttgaggcgc agggcatcga
     3601 gcgcctgccc ggtgttcacc tggggccgaa cgtggtggag atggaaggcc ggggcatccg
     3661 caccgaccgg gcagacgtgg ccctgaacat cgacaccgcc aacgcccaga tcatcgactt
     3721 acaggaatac cgggaggcaa tagaccatga acgcaatcga cagagtgaag aaatccagag
     3781 gcatcaacga gttagcggag cagatcgaac cgctggccca gagcatggcg acactggccg
     3841 acgaagcccg gcaggtcatg agccagaccc agcaggccag cgaggcgcag gcggcggagt
     3901 ggctgaaagc ccagcgccag acaggggcgg catgggtgga gctggccaaa gagttgcggg
     3961 aggtagccgc cgaggtgagc agcgccgcgc agagcgcccg gagcgcgtcg cgggggtggc
     4021 actggaagct atggctaacc gtgatgctgg cttccatgat gcctacggtg gtgctgctga
     4081 tcgcatcgtt gctcttgctc gacctgacgc cactgacaac cgaggacggc tcgatctggc
     4141 tgcgcttggt ggcccgatga agaacgacag gactttgcag gccataggcc gacagctcaa
     4201 ggccatgggc tgtgagcgct tcgatatcgg cgtcagggac gccaccaccg gccagatgat
     4261 gaaccgggaa tggtcagccg ccgaagtgct ccagaacacg ccatggctca agcggatgaa
     4321 tgcccagggc aatgacgtgt atatcaggcc cgccgagcag gagcggcatg gtctggtgct
     4381 ggtggacgac ctcagcgagt ttgacctgga tgacatgaaa gccgagggcc gggagcctgc
     4441 cctggtagtg gaaaccagcc cgaagaacta tcaggcatgg gtcaaggtgg ccgacgccgc
     4501 aggcggtgaa cttcgggggc agattgcccg gacgctggcc agcgagtacg acgccgaccc
     4561 ggccagcgcc gacagccgcc actatggccg cttggcgggc ttcaccaacc gcaaggacaa
     4621 gcacaccacc cgcgccggtt atcagccgtg ggtgctgctg cgtgaatcca agggcaagac
     4681 cgccaccgct ggcccggcgc tggtgcagca ggctggccag cagatcgagc aggcccagcg
     4741 gcagcaggag aaggcccgca ggctggccag cctcgaactg cccgagcggc agcttagccg
     4801 ccaccggcgc acggcgctgg acgagtaccg cagcgagatg gccgggctgg tcaagcgctt
     4861 cggtgatgac ctcagcaagt gcgactttat cgccgcgcag aagctggcca gccggggccg
     4921 cagtgccgag gaaatcggca aggccatggc cgaggccagc ccagcgctgg cagagcgcaa
     4981 gcccggccac gaagcggatt acatcgagcg caccgtcagc aaggtcatgg gtctgcccag
     5041 cgtccagctt gcgcgggccg agctggcacg ggcaccggca ccccgccagc gaggcatgga
     5101 caggggcggg ccagatttca gcatgtagtg cttgcgttgg tactcacgcc tgttatacta
     5161 tgagtactca cgcacagaag ggggttttat ggaatacgaa aaaagcgctt cagggtcggt
     5221 ctacctgatc aaaagtgaca agggctattg gttgcccggt ggctttggtt atacgtcaaa
     5281 caaggccgag gctggccgct tttcagtcgc tgatatggcc agccttaacc ttgacggctg
     5341 caccttgtcc ttgttccgcg aagacaagcc tttcggcccc ggcaagtttc tcggtgactg
     5401 atatgaaaga ccaaaaggac aagcagaccg gcgacctgct ggccagccct gacgctgtac
     5461 gccaagcgcg atatgccgag cgcatgaagg ccaaagggat gcgtcagcgc aagttctggc
     5521 tgaccgacga cgaatacgag gcgctgcgcg agtgcctgga agaactcaga gcggcgcagg
     5581 gcgggggtag tgaccccgcc agcgcctaac caccaactgc ctgcaaagga ggcaatcaat
     5641 ggctacccat aagcctatca atattctgga ggcgttcgca gcagcgccgc caccgctgga
     5701 ctacgttttg cccaacatgg tggccggtac ggtcggggcg ctggtgtcgc ccggtggtgc
     5761 cggtaaatcc atgctggccc tgcaactggc cgcacagatt gcaggcgggc cggatctgct
     5821 ggaggtgggc gaactgccca ccggcccggt gatctacctg cccgccgaag acccgcccac
     5881 cgccattcat caccgcctgc acgcccttgg ggcgcacctc agcgccgagg aacggcaagc
     5941 cgtggctgac ggcctgctga tccagccgct gatcggcagc ctgcccaaca tcatggcccc
     6001 ggagtggttc gacggcctca agcgcgccgc cgagggccgc cgcctgatgg tgctggacac
     6061 gctgcgccgg ttccacatcg aggaagaaaa cgccagcggc cccatggccc aggtcatcgg
     6121 tcgcatggag gccatcgccg ccgataccgg gtgctctatc gtgttcctgc accatgccag
     6181 caagggcgcg gccatgatgg gcgcaggcga ccagcagcag gccagccggg gcagctcggt
     6241 actggtcgat aacatccgct ggcagtccta cctgtcgagc atgaccagcg ccgaggccga
     6301 ggaatggggt gtggacgacg accagcgccg gttcttcgtc cgcttcggtg tgagcaaggc
     6361 caactatggc gcaccgttcg ctgatcggtg gttcaggcgg catgacggcg gggtgctcaa
     6421 gcccgccgtg ctggagaggc agcgcaagag caagggggtg ccccgtggtg aagcctaaga
     6481 acaagcacag cctcagccac gtccggcacg acccggcgca ctgtctggcc cccggcctgt
     6541 tccgtgccct caagcggggc gagcgcaagc gcagcaagct ggacgtgacg tatgactacg
     6601 gcgacggcaa gcggatcgag ttcagcggcc cggagccgct gggcgctgat gatctgcgca
     6661 tcctgcaagg gctggtggcc atggctgggc ctaatggcct agtgcttggc ccggaaccca
     6721 agaccgaagg cggacggcag ctccggctgt tcctggaacc caagtgggag gccgtcaccg
     6781 ctgaatgcca tgtggtcaaa ggtagctatc gggcgctggc aaaggaaatc ggggcagagg
     6841 tcgatagtgg tggggcgctc aagcacatac aggactgcat cgagcgcctt tggaaggtat
     6901 ccatcatcgc ccagaatggc cgcaagcggc aggggtttcg gctgctgtcg gagtacgcca
     6961 gcgacgaggc ggacgggcgc ctgtacgtgg ccctgaaccc cttgatcgcg caggccgtca
     7021 tgggtggcgg ccagcatgtg cgcatcagca tggacgaggt gcgggcgctg gacagcgaaa
     7081 ccgcccgcct gctgcaccag cggctgtgtg gctggatcga ccccggcaaa accggcaagg
     7141 cttccataga taccttgtgc ggctatgtct ggccgtcaga ggccagtggt tcgaccatgc
     7201 gcaagcgccg ccagcgggtg cgcgaggcgt tgccggagct ggtcgcgctg ggctggacgg
     7261 taaccgagtt cgcggcgggc aagtacgaca tcacccggcc caaggcggca ggctgacccc
     7321 ccccactcta ttgtaaacaa gacattttta tcttttatat tcaatggctt attttcctgc
     7381 taattggtaa taccatgaaa aataccatgc tcagaaaagg cttaacaata ttttgaaaaa
     7441 ttgcctactg agcgctgccg cacagctcca taggccgctt tcctggcttt gcttccagat
     7501 gtatgctctt ctgctcctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7561 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7621 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7681 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7741 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7801 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     7861 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7921 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7981 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8041 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8161 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     8221 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcgaat aaatacctgt
     8281 gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc
     8341 ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca
     8401 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc
     8461 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     8521 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     8581 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     8641 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
     8701 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     8761 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     8821 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     8881 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     8941 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     9001 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg
     9061 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     9121 gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg gtgctacgcc
     9181 tgaataagtg ataataagcg gatgaatggc agaaattcgt cgactctaga g
//
