LOCUS       40924_46143        5080 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVZ-CAM.fa, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Fraga D, Keenan E, Hendel E, Nair A, Schofield W.
  TITLE     The particle inflow gun can be used to co-transform Paramecium using
            Tungsten particles
  JOURNAL   J. Eukaryot. Microbiol. 53 (1), 16-19 (2006)
  PUBMED    16441576
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Fraga D.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2004) Biology, College of Wooster, 931 College 
            Mall, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5080)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5080)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Eukaryot. Microbiol."; date: "2006"; volume: "53"; issue: "1"; 
            pages: "16-19"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-MAY-2004) Biology, College of Wooster, 931 College Mall, 
            Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          930..2779
                     /mol_type="other DNA"
                     /db_xref="taxon:5888"
                     /organism="Paramecium tetraurelia"
     rep_origin      complement(237..692)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     837..853
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        860..878
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1557..2006)
                     /codon_start=1
                     /product="calmodulin"
                     /label=calmodulin
                     /protein_id="AAT38517.1"
                     /translation="MAE*LTEE*IAEFKEAFALFDKDGDGTITTKELGTVMRSLG*NPT
                     EAELQDMINEVDADGNGTIDFPEFLSLMARKMKE*DSEEELIEAFKVFDRDGNGLISAA
                     ELRHVMTNLGEKLTDDEVDEMIREADIDGDGHINYEEFVRMMVSK"
     promoter        complement(2820..2838)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2859..2875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2883..2899)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2907..2937)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2952..2973)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3261..3849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4023..4880)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4881..4985)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
      241 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
      301 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
      361 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
      421 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
      481 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
      541 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
      601 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
      661 cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat tcgccattca ggctacgcaa
      721 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaagggggg
      781 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      841 aacgacggcc agtgaattgt aatacgactc actatagggc gaattgggcc caatgcattg
      901 gcgccgcggc cgcgaattcg agctcggtac ccggggatct ctatgttaaa aattggatcc
      961 aaattaataa tacaaaaatt gatatccttc tatgatttat cgaattatat caagaacatt
     1021 acaataaaat tgataattct ttacttaaaa tgaggtgact gtcttctaat ataangtaga
     1081 tagattagat tcacaatttt acatcacaat gaaagtacta tcattctcat caaacaagtc
     1141 ataatattca atgatgtgag gattatagtg attaatttta tataaattaa ttagatcctt
     1201 ggttatttat ttctctctct ttggttctaa taatattttg caattacctc tctatatttt
     1261 ttagactaat tagtgattta cttaataaca atttttctat tattgtgttt tatatcttca
     1321 gctataaaga tacgatcctt tatcttagaa caaaactcgt agtgtttatt actccaatac
     1381 tttatgttgt gaaatccata tgatgtccca ngtattgttt gacataagta taaaaaataa
     1441 aaataatagt aattagaaaa tttcgagatt tatttaaata taagtacgtt ctttaattta
     1501 tatttattta agtgttgttc attttgataa atatcttaat aattatgata taataatcat
     1561 ttagaaacca tcattctgac gaattcttca tagttgatat gtccatctcc atcaatatca
     1621 gcttctctga tcatttcatc aacctcatcg tcagttaatt tttctcccaa atttgtcata
     1681 acatgtctca attcagcagc agaaatgagt ccgtttccat ctctatcaaa gactttgaaa
     1741 gcttcaatta attcttcctc agaatcttat tccttcattt ttctagccat caaagataaa
     1801 aattcaggga aatcgattgt tccattaccg tcagcatcga cttcgttgat catatcttga
     1861 agttcagctt cagttgggtt ttatccaaga gatctcataa ctgttcccaa ttctttagtt
     1921 gtaatggtac catcaccatc cttatcaaag agagcaaagg cttctttgaa ttcagcaatt
     1981 tattcttctg ttaattattc agccatttat tagttgattt atttaattaa tgatttgata
     2041 cttagataat tgacttataa atcaaatttg aatttaacaa agcatattta caaaacacaa
     2101 aaaagcaatt aattaantat aagaattcat aagaaaattt ataatgcaaa taatattgga
     2161 atacatatcc taatatcatt atattccatt catcttaata atatgaaatg ataatctaaa
     2221 ccaaagaata actgattctg atagttttgc aaagcaatat taattctttg aaaaatttca
     2281 ttttttcttt aaacttatgt tgtatctgaa gatcattcct atatccttta agttgaattc
     2341 atggagttta aatggtaaat ttcacttatt atcacaattg atttataaag aattgaaaac
     2401 gaatgaataa ttctaattgc catttaaaat aatttcttac aaagaaaaat gtatataaaa
     2461 aggttcagaa gggttataat taaactactg tttgatattt ttttcttatt tactttttca
     2521 ttcaatcagc ttanatattg aattataaat attttctgaa tactaaacgt ttgatgtatt
     2581 tatgcctcta taatgttctt atcaaacaca ataacttggt gtttatctgg aaattctcag
     2641 aaaaacatac acttcagctt taatctcaga attttttctt aaatataaga atataactta
     2701 ataaaatcaa gtcttaattt aataaagtag aatcctaata taaattatta atcttatctt
     2761 ctgagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag cttttgttcc
     2821 ctttagtgag ggttaattcc gagcttggcg taatcatggt catagctgtt tcctgtgtga
     2881 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2941 tggggtgcct aatgagtgag gtaactcaca ttaattgcgt tgcgctcact gcccgctttc
     3001 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     3061 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3241 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3421 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     3481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3661 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     3721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4021 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4081 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4141 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4201 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4261 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4321 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4381 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4441 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4501 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4561 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4621 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4681 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4741 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4801 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4861 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4921 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4981 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     5041 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
