LOCUS       40924_46138        8619 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVWEx1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Bekel T, Stadermann KB, Voss M.
  TITLE     Nucleotide sequence analysis of the plasmid pVWEx1 by Illumina 
            sequencing technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8619)
  AUTHORS   Bekel T, Stadermann KB, Voss M.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 8619)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8619)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-APR-2017) Evonik Nutrition "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 9
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..35
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    43..59
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    complement(105..161)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(162..178)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      391..477
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      569..596
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        615..706
                     /label=AmpR promoter
     rep_origin      924..3989
                     /label=derived from Corynebacterium glutamicum pCG1
                     /note="derived from Corynebacterium glutamicum pCG1"
     CDS             complement(4575..5387)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(5982..6527)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(7235..8314)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(8315..8392)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttcacacagg aaacagaatt aaaagatatg accatgatta cgccaagctt gcatgcctgc
      121 aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cactggccgt cgttttacag
      181 ccaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag
      241 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
      301 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc
      361 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
      421 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
      481 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
      541 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
      601 tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      661 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      721 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      781 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      841 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      901 aatgatgagc acttttgatc cccctgcggc gtcgctgatc gccctcgcga cgttgtgcgg
      961 gtggcttgtc cctgagggcg ctgcgacaga tagctaaaaa tctgcgtcag gatcgccgta
     1021 gagcgcgcgt cgcgtcgatt ggaggcttcc cctttggttg acggtcttca atcgctctac
     1081 ggcgatcctg acgctttttt gttgcgtacc gtcgatcgtt ttatttctgt cgatcccgaa
     1141 aaagtttttg ccttttgtaa aaaacttctc ggtcgccccg caaattttcg attccagatt
     1201 ttttaaaaac caagccagaa atacgacaca ccgtttgcag ataatctgtc tttcggaaaa
     1261 atcaagtgcg atacaaaatt tttagcaccc ctgagctgcg caaagtcccg cttcgtgaaa
     1321 attttcgtgc cgcgtgattt tccgccaaaa actttaacga acgttcgtta taatggtgtc
     1381 atgaccttca cgacgaagta ccaaaattgg cccgaatcat cagctatgga tctctctgat
     1441 gtcgcgctgg agtccgacgc gctcgatgct gccgtcgatt taaaaacggt gatcggattt
     1501 ttccgagctc tcgatacgac ggacgcgcca gcatcacgag actgggccag tgccgcgagc
     1561 gacctagaaa ctctcgtggc ggatcttgag gagctggctg acgagctgcg tgctcggcag
     1621 cgccaggagg acgcacagta gtggaggatc gaatcagttg cgcctactgc ggtggcctga
     1681 ttcctccccg gcctgacccg cgaggacggc gcgcaaaata ttgctcagat gcgtgtcgtg
     1741 ccgcagccag ccgcgagcgc gccaacaaac gccacgccga ggagctggag gcggctaggt
     1801 cgcaaatggc gctggaagtg cgtcccccga gcgaaatttt ggccatggtc gtcacagagc
     1861 tggaagcggc agcgagaatt atccgcgatc gtggcgcggt gcccgcaggc atgacaaaca
     1921 tcgtaaatgc cgcgtttcgt gtggccgtgg ccgcccagga cgtgtcagcg ccgccaccac
     1981 ctgcaccgaa tcggcagcag cgtcgcgcgt cgaaaaagcg cacaggcggc aagaagcgat
     2041 aagctgcacg aatacctgaa aaatgttgaa cgccccgtga gcggtaactc acagggcgtc
     2101 ggctaacccc cagtccaaac ctgggagaaa gcgctcaaaa atgactctag cggattcacg
     2161 agacattgac acaccggcct ggaaattttc cgctgatctg ttcgacaccc atcccgagct
     2221 cgcgctgcga tcacgtggct ggacgagcga agaccgccgc gaattcctcg ctcacctggg
     2281 cagagaaaat ttccagggca gcaagacccg cgacttcgcc agcgcttgga tcaaagaccc
     2341 ggacacggga gaaacacagc cgaagttata ccgagttggt tcaaaatcgc ttgcccggtg
     2401 ccagtatgtt gctctgacgc acgcgcagca cgcagccgtg cttgtcctgg acattgatgt
     2461 gccgagccac caggccggcg ggaaaatcga gcacgtaaac cccgaggtct acgcgatttt
     2521 ggagcgctgg gcacgcctgg aaaaagcgcc agcttggatc ggcgtgaatc cactgagcgg
     2581 gaaatgccag ctcatctggc tcattgatcc ggtgtatgcc gcagcaggca tgagcagccc
     2641 gaatatgcgc ctgctggctg caacgaccga ggaaatgacc cgcgttttcg gcgctgacca
     2701 ggctttttca cataggctga gccggtggcc actgcacgtc tccgacgatc ccaccgcgta
     2761 ccgctggcat gcccagcaca atcgcgtgga tcgcctagct gatcttatgg aggttgctcg
     2821 catgatctca ggcacagaaa aacctaaaaa acgctatgag caggagtttt ctagcggacg
     2881 ggcacgtatc gaagcggcaa gaaaagccac tgcggaagca aaagcacttg ccacgcttga
     2941 agcaagcctg ccgagcgccg ctgaagcgtc tggagagctg atcgacggcg tccgtgtcct
     3001 ctggactgct ccagggcgtg ccgcccgtga tgagacggct tttcgccacg ctttgactgt
     3061 gggataccag ttaaaagcgg ctggtgagcg cctaaaagac accaagatca tcgacgccta
     3121 cgagcgtgcc tacaccgtcg ctcaggcggt cggagcagac ggccgtgagc ctgatctgcc
     3181 gccgatgcgt gaccgccaga cgatggcgcg acgtgtgcgc ggctacgtcg ctaaaggcca
     3241 gccagtcgtc cctgctcgtc agacagagac gcagagcagc cgagggcgaa aagctctggc
     3301 cactatggga agacgtggcg gtaaaaaggc cgcagaacgc tggaaagacc caaacagtga
     3361 gtacgcccga gcacagcgag aaaaactagc taagtccagt caacgacaag ctaggaaagc
     3421 taaaggaaat cgcttgacca ttgcaggttg gtttatgact gttgagggag agactggctc
     3481 gtggccgaca atcaatgaag ctatgtctga atttagcgtg tcacgtcaga ccgtgaatag
     3541 agcacttaag tctgcgggca ttgaacttcc acgaggacgc cgtaaagctt cccagtaaat
     3601 gtgccatctc gtaggcagaa aacggttccc cccgtagggg tctctctctt ggcctccttt
     3661 ctaggtcggg ctgattgctc ttgaagctct ctaggggggc tcacaccata ggcagataac
     3721 ggttccccac cggctcacct cgtaagcgca caaggactgc tcccaaagat cttcaaagcc
     3781 actgccgcga ctccgcttcg cgaagccttg ccccgcggaa atttcctcca ccgagttcgt
     3841 gcacacccct atgccaagct tctttcaccc taaattcgag agattggatt cttaccgtgg
     3901 aaattcttcg caaaaatcgt cccctgatcg cccttgcgac gttgctcgcg gcggtgccgc
     3961 tggttgcgct tggcttgacc gacttgatcc tccggcgttc agcctgtgcc acagccgaca
     4021 ggatggtgac caccatttgc cccatatcac cgtcggtact gatcccgtcg tcaataaacc
     4081 gaaccgctac accctgagca tcaaactctt ttatcagttg gatcatgtcg gcggtgtcgc
     4141 ggccaagacg gtcgagcttc ttcaccagaa tgacatcacc ttcctccacc ttcatcctca
     4201 gcaaatccag cccttcccga tctgttgaac tgccggatgc cttgtcggta aagatgcggt
     4261 tagcttttac ccctgcatct ttgagcgctg aggtctgcct cgtgaagaag gtgttgctga
     4321 ctcataccag gcctgaatcg ccccatcatc cagccagaaa gtgagggagc cacggttgat
     4381 gagagctttg ttgtaggtgg accagttggt gattttgaac ttttgctttg ccacggaacg
     4441 gtctgcgttg tcgggaagat gcgtgatctg atccttcaac tcagcaaaag ttcgatttat
     4501 tcaacaaagc cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta caaccaatta
     4561 accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     4621 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     4681 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     4741 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     4801 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     4861 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     4921 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     4981 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     5041 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     5101 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     5161 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     5221 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     5281 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     5341 tttaatcgcg gcctcgagca agacgtttcc cgttgaatat ggctcataac accccttgta
     5401 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     5461 atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct
     5521 gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa
     5581 aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc
     5641 actttctggc tggatgatgg ggcgattcag gcctggtatg agtcagcaac accttcttca
     5701 cgaggcagac ctcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg
     5761 gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa
     5821 tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
     5881 actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa
     5941 gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc
     6001 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
     6061 taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
     6121 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
     6181 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     6241 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
     6301 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
     6361 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
     6421 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
     6481 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
     6541 ttaaggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6601 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6661 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6721 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6781 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6841 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6901 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6961 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgcaaac atggcctgtc
     7021 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     7081 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     7141 cgtcttgctg gcgttcggaa cgccagcaag acgtagccca gcgcgtcggc cagcttgcaa
     7201 ttcgcgctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7261 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7321 gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg
     7381 cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat
     7441 cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc
     7501 ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc
     7561 ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca
     7621 tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg
     7681 gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga
     7741 cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct
     7801 ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt
     7861 cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat
     7921 cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt
     7981 gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg
     8041 cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg
     8101 ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca
     8161 cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg
     8221 cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat
     8281 actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt
     8341 ccgggcgcta tcatgccata ccgcgaaagg ttttgcacca ttcgatggtg tcaacgtaaa
     8401 tgcatgccgc ttcgccttcg cgcgcgaatt gcaagctgat ccgggcttat cgactgcacg
     8461 gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc
     8521 gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt
     8581 gcgccgacat cataacggtt ctggcaaata ttctgaaat
//
