LOCUS       40924_46128        7357 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pVTLU260, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Mok CSL., Melcher K.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2005) School of Biomedical Sciences, University of
            Ulster, Cromore Road, Coleraine BT52 1SA, UK
REFERENCE   2  (bases 1 to 7357)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7357)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-MAR-2005) School of Biomedical Sciences, University of Ulster, 
            Cromore Road, Coleraine BT52 1SA, UK"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..244
                     /label=URA3 promoter
     CDS             245..1045
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        1246..1350
                     /label=AmpR promoter
     CDS             1351..2208
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDVGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2382..2970
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2999..3400
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             3412..3429
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             3436..3456
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     terminator      3639..3826
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     rep_origin      3837..5183
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             5557..6648
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEALRYPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPGFAKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     7319..7335
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgacctgca ggcatgcaag cttttcaatt caattcatca tttttttttt attctttttt
       61 ttgatttcgg tttctttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg
      121 aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg
      181 aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga aacgaagata
      241 aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc
      301 caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg
      361 taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa
      421 aacacatgtg gatatcttga ctgatttttc aatggagggc acagttaagc cgctaaaggc
      481 attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa
      541 tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac
      601 gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga
      661 agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct
      721 atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt
      781 tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat
      841 tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac
      901 cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag gactatttgc
      961 aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata
     1021 tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact
     1081 aaactcacaa attagagctt caatttaatt atatcagtta ttacccgggt accgagctcg
     1141 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1201 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1261 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1321 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1381 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1441 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1501 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1561 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg tcgggcaaga gcaactcggt
     1621 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1681 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1741 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1801 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1861 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1921 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1981 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     2041 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     2101 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2161 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2221 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2281 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2341 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2401 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2461 gatcaagagc tatcaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2521 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2581 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2641 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2701 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2761 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2821 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2881 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2941 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcccg acctcgacgc
     3001 atgcaacttc ttttcttttt ttttcttttc tctctccccc gttgttgtct caccatatcc
     3061 gcaatgacaa aaaaaatgat ggaagacact aaaggaaaaa attaacgaca aagacagcac
     3121 caacagatgt cgttgttcca gagctgatga ggggtatctt cgaacacacg aaactttttc
     3181 cttccttcat tcacgcacac tactctctaa tgagcaacgg tatacggcct tccttccagt
     3241 tacttgaatt tgaaataaaa aaagtttgcc gctttgctat caagtataaa tagacctgca
     3301 attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt gtttcttttt
     3361 ctgcacaata tttcaagcta taccaagcat acaatcaact ccaagcttat gcaccatcac
     3421 catcaccatg ctagcgagaa tctttatttt cagggcgcca tggtctagca tgactggtgg
     3481 acagcaaatg ggtcggggat ccagctttgg acttcttcgc cagaggtttg gtcaagtctc
     3541 caatcaaggt tgtcggcttg tctaccttgc cagaaattta cgaaaagatg gaaaagggtc
     3601 aaatcgttgg tagatacgtt gttgacactt ctaaataagc gaatttctta tgatttatga
     3661 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
     3721 aggttttaaa acgaaaattc ttgttcttga gtaactcttt cctgtaggtc aggttgcttt
     3781 ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg cgatatgatc
     3841 caatatcaaa ggaaatgata gcattgaagg atgagactaa tccaattgag gagtggcagc
     3901 atatagaaca gctaaagggt agtgctgaag gaagcatacg ataccccgca tggaatggga
     3961 taatatcaca ggaggtacta gactaccttt catcctacat aaatagacgc atataagtac
     4021 gcatttaagc ataaacacgc actatgccgt tcttctcatg tatatatata tacaggcaac
     4081 acgcagatat aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt
     4141 cggaagcgct cgttttcgga aacgctttga agttcctatt ccgaagttcc tattctctag
     4201 ctagaaagta taggaacttc agagcgcttt tgaaaaccaa aagcgctctg aagacgcact
     4261 ttcaaaaaac caaaaacgca ccggactgta acgagctact aaaatattgc gaataccgct
     4321 tccacaaaca ttgctcaaaa gtatctcttt gctatatatc tctgtgctat atccctatat
     4381 aacctaccca tccacctttc gctccttgaa cttgcatcta aactcgacct ctacattttt
     4441 tatgtttatc tctagtatta ctctttagac aaaaaaattg tagtaagaac tattcataga
     4501 gtgaatcgaa aacaatacga aaatgtaaac atttcctata cgtagtatat agagacaaaa
     4561 tagaagaaac cgttcataat tttctgacca atgaagaatc atcaacgcta tcactttctg
     4621 ttcacaaagt atgcgcaatc cacatcggta tagaatataa tcggggatgc ctttatcttg
     4681 aaaaaatgca cccgcagctt cgctagtaat cagtaaacgc gggaagtgga gtcaggcttt
     4741 ttttatggaa gagaaaatag acaccaaagt agccttcttc taaccttaac ggacctacag
     4801 tgcaaaaagt tatcaagaga ctgcattata gagcgcacaa aggagaaaaa aagtaatcta
     4861 agatgctttg ttagaaaaat agcgctctcg ggatgcattt ttgtagaaca aaaaagaagt
     4921 atagattctt tgttggtaaa atagcgctct cgcgttgcat ttctgttctg taaaaatgca
     4981 gctcagattc tttgtttgaa aaattagcgc tctcgcgttg catttttgtt ttacaaaaat
     5041 gaagcacaga ttcttcgttg gtaaaatagc gctttcgcgt tgcatttctg ttctgtaaaa
     5101 atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt ttgttctaca
     5161 aaatgaagca cagatgcttc gttaacaaag atatgctatt gaagtgcaag atggaaacgc
     5221 agaaaatgaa ccggggatgc gacgtgcaag attacctatg caatagatgc aatagtttct
     5281 ccaggaaccg aaatacatac attgtcttcc gtaaagcgct agactatata ttattataca
     5341 ggttcaaata tactatctgt ttcagggaaa actcccaggt tcggatgttc aaaattcaat
     5401 gatgggtaac aagtacgatc gtaaatctgt aaaacagttt gtcggatatt aggctgtatc
     5461 tcctcaaagc gtattcgaat atcattgaga agctgcattt tttttttttt tttttttttt
     5521 ttttttttat atatatttca aggatatacc attgtaatgt ctgcccctaa gaagatcgtc
     5581 gttttgccag gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa
     5641 gctatttctg atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt
     5701 gctgctatcg atgctacagg tgtcccactt ccagatgagg cgctggaagc ctccaagaag
     5761 gttgatgccg ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga
     5821 cctgaacaag gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca
     5881 tgtaactttg catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa
     5941 ggtactgact tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag
     6001 gaagacgatg gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa
     6061 agaatcacaa gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg
     6121 tccttggata aagctaatgt tttggcctct tcaagattat ggagaaaaac tgtggaggaa
     6181 accatcaaga acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc
     6241 atgatcctag ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt
     6301 ggtgatatca tctccgatga agccttacgt tatccaggtt ccttgggttt gttgccatct
     6361 gcgtccttgg cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac
     6421 ggttctgctc ccggatttgc caagaataag gttgacccta tcgccactat cttgtctgct
     6481 gcaatgatgt tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca
     6541 gttaaaaagg ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc
     6601 accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct
     6661 cttttttatg atatttgtac ataaacttta taaatgaaat tcataataga aacgacacga
     6721 aattacaaaa tggaatatgt tcatagggta gacaaacgca atctacatac atttatcaag
     6781 aaggagaaaa aggaggatag ctaaaggaat acaggtaagc aaattgatac taatggctca
     6841 acgtgataag gaaagagaat tgcactttaa cattaatatt gacaaggagg agggcaccac
     6901 acaaaaagtt aggtataaca gaaaatcatg aaactacgaa tccctaattt gatattggag
     6961 gattttctct aaaaaaaaaa aaatacaaca aataaaaaac actcaaatga cctgaccatt
     7021 tgatggagtt taagtcaata ccttcttgaa ccattttcca taatggtgaa agttccctca
     7081 agaattttac tctgtcagaa acggccttaa cgacgtaccg gatctatgcg gtgtgaaata
     7141 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctacgc
     7201 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaagggg
     7261 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     7321 aaaacgacgg ccagtgccaa gcttgcatgc ctgcagg
//
