LOCUS       Exported                5892 bp DNA     circular SYN 17-AUG-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        166..545
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        546..749
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        711..749
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          861..1433
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1503..3038
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    3239..3633
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     misc_feature    3451..3457
                     /label=SapI
     rep_origin      complement(4143..4731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4902..5762)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5763..5867)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtggatc ccctgagggg gcccccatgg gctagaggat ccggcctcgg cctctgcata
       61 aataaaaaaa attagtcagc catgagcttg gcccattgca tacgttgtat ccatatcata
      121 atatgtacat ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga
      181 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      241 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      301 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      361 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      421 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      481 acatgacctt atgggacttt cctacttggc agtacatcta catattagtc atcgctatta
      541 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      601 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      661 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      721 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
      781 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggtcga
      841 ccgatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta
      901 ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa
      961 gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct
     1021 gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact
     1081 ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt
     1141 actttctcta atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac
     1201 agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt
     1261 ctggctggcg tggaaatatt cttattggta gaaacaacta caccctggtc atcatcctgc
     1321 ctttctcttt atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt
     1381 ccaaaccggg cccctctgct aaccatgttc atgccttctt ctctttccta cagctcctgg
     1441 gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa agaattcctc gacggatccg
     1501 ccatgaagtg ccttttgtac ttagcctttt tattcattgg ggtgaattgc aagttcacca
     1561 tagtttttcc acacaaccaa aaaggaaact ggaaaaatgt tccttctaat taccattatt
     1621 gcccgtcaag ctcagattta aattggcata atgacttaat aggcacagcc ttacaagtca
     1681 aaatgcccaa gagtcacaag gctattcaag cagacggttg gatgtgtcat gcttccaaat
     1741 gggtcactac ttgtgatttc cgctggtatg gaccgaagta tataacacat tccatccgat
     1801 ccttcactcc atctgtagaa caatgcaagg aaagcattga acaaacgaaa caaggaactt
     1861 ggctgaatcc aggcttccct cctcaaagtt gtggatatgc aactgtgacg gatgccgaag
     1921 cagtgattgt ccaggtgact cctcaccatg tgctggttga tgaatacaca ggagaatggg
     1981 ttgattcaca gttcatcaac ggaaaatgca gcaattacat atgccccact gtccataact
     2041 ctacaacctg gcattctgac tataaggtca aagggctatg tgattctaac ctcatttcca
     2101 tggacatcac cttcttctca gaggacggag agctatcatc cctgggaaag gagggcacag
     2161 ggttcagaag taactacttt gcttatgaaa ctggaggcaa ggcctgcaaa atgcaatact
     2221 gcaagcattg gggagtcaga ctcccatcag gtgtctggtt cgagatggct gataaggatc
     2281 tctttgctgc agccagattc cctgaatgcc cagaagggtc aagtatctct gctccatctc
     2341 agacctcagt ggatgtaagt ctaattcagg acgttgagag gatcttggat tattccctct
     2401 gccaagaaac ctggagcaaa atcagagcgg gtcttccaat ctctccagtg gatctcagct
     2461 atcttgctcc taaaaaccca ggaaccggtc ctgctttcac cataatcaat ggtaccctaa
     2521 aatactttga gaccagatac atcagagtcg atattgctgc tccaatcctc tcaagaatgg
     2581 tcggaatgat cagtggaact accacagaaa gggaactgtg ggatgactgg gcaccatatg
     2641 aagacgtgga aattggaccc aatggagttc tgaggaccag ttcaggatat aagtttcctt
     2701 tatacatgat tggacatggt atgttggact ccgatcttca tcttagctca aaggctcagg
     2761 tgttcgaaca tcctcacatt caagacgctg cttcgcaact tcctgatgat gagagtttat
     2821 tttttggtga tactgggcta tccaaaaatc caatcgagct tgtagaaggt tggttcagta
     2881 gttggaaaag ctctattgcc tcttttttct ttatcatagg gttaatcatt ggactattct
     2941 tggttctccg agttggtatc catctttgca ttaaattaaa gcacaccaag aaaagacaga
     3001 tttatacaga catagagatg aaccgacttg gaaagtaact caaatcctgc acaacagatt
     3061 cttcatgttt ggaccaaatc aacttgtgat accatgctca aagaggcctc aattatattt
     3121 gagtttttaa tttttatgaa aaaaaaaaaa aaaaacggaa ttcaccccac cagtgcaggc
     3181 tgcctatcag aaagtggtgg ctggtgtggc taatgccctg gcccacaagt atcactaagc
     3241 tcgctttctt gctgtccaat ttctattaaa ggttcctttg ttccctaagt ccaactacta
     3301 aactggggga tattatgaag ggccttgagc atctggattc tgcctaataa aaaacattta
     3361 ttttcattgc aatgatgtat ttaaattatt tctgaatatt ttactaaaaa gggaatgtgg
     3421 gaggtcagtg catttaaaac ataaagaaat gaagagctag ttcaaacctt gggaaaatac
     3481 actatatctt aaactccatg aaagaaggtg aggctgcaaa cagctaatgc acattggcaa
     3541 cagcccctga tgcctatgcc ttattcatcc ctcagaaaag gattcaagta gaggcttgat
     3601 ttggaggtta aagttttgct atgctgtatt ttacattact tattgtttta gctgtcctca
     3661 tgaatgtctt ttcactaccc atttgcttat cctgcatctc tcagccttga ctccactcag
     3721 ttctcttgct tagagatacc acctttcccc tgaagtgttc cttccatgtt ttacggcgag
     3781 atggtttctc ctcgcctggc cactcagcct tagttgtctc tgttgtctta tagaggtcta
     3841 cttgaagaag gaaaaacagg gggcatggtt tgactgtcct gtgagccctt cttccctgcc
     3901 tcccccactc acagtgaccc ggaatccctc gacatggcag tctagcacta gtgcggccgc
     3961 agatctgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4021 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4081 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4141 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4261 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4321 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4381 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4441 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4501 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4561 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4621 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4681 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4741 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4801 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4861 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4921 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4981 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5041 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5101 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5161 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5221 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5281 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5341 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5401 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5461 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5521 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5581 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5641 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5701 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5761 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5821 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5881 aaagtgccac ct
//
