LOCUS       V012181                11340 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V012181
VERSION     V012181
KEYWORDS    pVQ CMV NanoV1-2a-EGFP ferritin
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Stanley SA, Kelly L, Latcha KN, Schmidt SF, Yu X, Nectow AR, Sauer
            J, Dyke JP, Dordick JS, Friedman JM
  TITLE     Bidirectional electromagnetic control of the hypothalamus regulates
            feeding and metabolism.
  JOURNAL   Nature. 2016 Mar 31;531(7596):647-50. doi: 10.1038/nature17183. Epub
            2016 Mar 23.
   PUBMED   27007848
REFERENCE   2  (bases 1 to 11340)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11340)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi:
            "10.1038/nature17183"; journalName: "Nature"; date: "2016-03-31-
            31"; volume: "531"; issue: "7596"; pages: "647-50"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     389..411
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     polyA_signal    554..688
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1328..1351)
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     RBS             1506..1514
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(1928..2644)
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             complement(2660..2713)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2714..5227)
                     /gene="Trpv1"
                     /label="Transient receptor potential cation channel
                     subfamily V member 1"
                     /note="Transient receptor potential cation channel
                     subfamily V member 1 from Rattus norvegicus. Accession#:
                     O35433"
     CDS             complement(5258..5599)
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /label="GFP nanobody"
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYSNVNVGFEYW
                     GQGTQVTVSS"
     regulatory      5605..5614
                     /label="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5643..5667)
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5694..5727)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(5775..5978)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(5979..6282)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(6372..6392)
                     /label="attB4"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     primer_bind     complement(6418..6434)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(6419..6435)
                     /label="pBluescriptKS"
                     /note="For pBluescript vector"
     misc_signal     complement(6476..6626)
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   6714..6816
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     primer_bind     complement(6844..6862)
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     promoter        6930..7034
                     /label="AmpR promoter"
     CDS             7035..7892
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8066..8654
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             9507..10841
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     CDS             complement(10907..11326)
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
ORIGIN
        1 ctgcaaaaca gatacaaaac tacataagac ccccacctta tatattcttt cccaccctta
       61 agccacgccc acacatttca gtacctcaat ctgtatcttc atcgctagag ccaaactcag
      121 cgcgggtgca ggccagcacc aagtgatcgg gcctcagctc ctcggtcaca tccagcatca
      181 caggctggtt cctaatatgt ttaccgccac actcgcaggg tctgcacctg gtgcgggtct
      241 catcgtacct cagcaccttc cagatctgcc accgcgggga ctagagtcga cctcatggct
      301 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
      361 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
      421 tcatcaccga aacgcgcgag gcagccggat cataatcagc cataccacat ttgtagaggt
      481 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
      541 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
      601 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      661 catcaatgta tcttatcatg tctggatctc cccgcggtgg cggccgctcg agtctagagg
      721 ccggccaagc ttttactatt ccggactatt attaacgcgt gctctcatca ccgtgtccca
      781 gggtgtgctt gtcaaagaga tattctgcca tgccagcttc aggggcaccc atcttgcgta
      841 agttggtcac gtggtcaccc agttctttaa tggatttcac ctgttcactc agataatacg
      901 tctcaatgaa gtcacataag tggggatcat tcttgtcagt agccagtttg tgcagttcca
      961 gtagtgactg attcacactc ttttccaagt gcagtgcaca ctccattgca ttcagcccgc
     1021 tctcccagtc atcacggtct ggtttcttta tatcctgcag gaagattcgg ccacctcgct
     1081 ggttctgcag cttcatcagt ttctcggcat gctccctctc ctcatgagat tggtggagaa
     1141 agtatttggc aaagttcttc agagccacat catctcggtc aaaataacaa gacatagaca
     1201 gatagacgta ggaggcatac aactccaggt tgatctggcg gttgatggca gcctccgcgt
     1261 cctggtggta gttctggcgc acttgcgagg gagacgcggt ggtcatcacg cgggagccgc
     1321 cgccgccctt gtcgtcgtcg tccttgtagt cggagccgcc gccgccgcgg gcgtcgtgct
     1381 tgagagtgag gcgctcaaag agatactcgc ccagagaccc ctggggcgcg ccagtctgcg
     1441 ctggttgtgg ccccgccacc ctgcggaggt tggtcagatg gttgcccatc ttcttgatga
     1501 gtttcacctc cttatccaga tagtggcttt ccaggaagtc acagagatga gggtccgcgc
     1561 gggcagaacc cagggcatgc agatccaaga gggcctgatt caggttcttc tccatggcca
     1621 aggcagcttc catggcctcc tgggttttac cccattcatc ttgagatggc ttctgcacat
     1681 cctggaagag tgcacggccc ccgcgatcgt tctgaaactc gaggagacgc tccgcgccct
     1741 cgcgcttctc ctcggccaat tcgcggaaga agtggcctac gccctccaga gccacgtcat
     1801 cccgatcaaa aaagaagccc agagagaggt aggtgtagga ggcccgcagg tgcaagttga
     1861 ccaggcggtt cacggcagct tccacctcgg tggaataatt ctgacgaatc tgagaggtca
     1921 tgacgtcctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
     1981 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
     2041 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
     2101 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga
     2161 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
     2221 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
     2281 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
     2341 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
     2401 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
     2461 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
     2521 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
     2581 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
     2641 ccatggtgct agcagatctt gggccaggat tctcctcgac gtcaccgcat gttagcagac
     2701 ttcctctgcc ctccttctcc cctgggacca tggaatcctt gaaaacctca gcatcctctg
     2761 gcttaaggga tcccgtataa tgcttcagtt gaacttcttc ctgctgggtg gcatgtctat
     2821 ctcgagtgct tgcatccctc agaaggggaa ccagggcaaa gttcttccag tttctccctg
     2881 aaactcggcc tgacctcagg gagaagctca gggtgcgctt gacgccctca cagttgcctg
     2941 ggtcctcgtt gatgataccc acattggtgt tccaggtagt ccagtttacc tcgtccaccc
     3001 tgaaacacca ccggtagtca tccttgccgt caggagtgaa ccccacctgc agcagcttgc
     3061 cagagcggaa ggccttcctc atgcacttca ggaagctctt ctctgtatcc aggatggtga
     3121 tggctctctg cagcttccag atgttcttgc tctcttgtgc aatcttgttg acggtctcac
     3181 ccatgagagc aatgagcatg ttgagcagaa ggatgtaggt gagaatcaca taggccagta
     3241 acaggatgat gaagacagcc ttgaagtcgt agttctcagt gaactccagg tcgcccatgc
     3301 cgatggtgaa cttgaacagc tccagacatg tggaatacag gctgttgtaa gagttacctg
     3361 gcttgcaggc agacccccgg cacttgtgtg gtgtggactc cataggcaga gagttattct
     3421 tcccatcctc aatcagtgtc accacagctg tggaaaatcc aaacaagaac acgaggtaga
     3481 cgaacataaa ccggcatagg tctctgagga tcatcttctc aatcatgaca gcatagatgc
     3541 ccatctgctg gaatcctcgg gtatagtaga gcatgttggt ccagcccatg gccagggaga
     3601 acaccatgga agccacatac tccttgcgtt ggctgaagta cagtaccaca gacaccagca
     3661 tgaacagcga ctgtacaaag aaaagtatct cactgtagct gtccacaaac aaactcttga
     3721 gggatggtcg cctctgcagg aaatattgaa tccctcggaa gaagaagtag actcctcctg
     3781 acacagacaa gatctctccg gtgactcgga aatagtcccc aacggtgttt ttcagcttat
     3841 aggggggcaa gccttccaca ggccgatagt aggcagccgc ggtgaagatg atcatataca
     3901 agtagtagac gaagaagttg aagtagaaga tgcgcttgac aaatctgtcc cacttgtcct
     3961 gtaggagtcg gttcaagggt tccacgagaa gcatgtcatg acggttaggg gtctcactgc
     4021 tgctgtaagc gatcacctcc agaaccgagt tcttttcaca ggtgtcaatg caggacaggt
     4081 cataaaggga ggagtacact ggcccatagg cccattcggt gaacttcctg gataggtgtc
     4141 ggcactcggg ttcatggatc tccctctgga gaatgtaggc caagaccccg atcttcccac
     4201 tgctagcagc cagagccagt ggcgtgagcc ccttcctgtt ggtgatctct tccagcttca
     4261 gcgtggggtg gagtttggcc cccaggatca agatctcgtt gtacatgctt gtcacgaact
     4321 tggtgttgtc aactgtgtta tctgccacct ccaccagggc atgaagcacc gtgttgccca
     4381 ctgagtcccg ggcgctgatg tctgcaggct gccaggagtt ctgcagcagg aacttcacaa
     4441 tggccagctg gttggtgcac gcagccaggg acaggggcag ctcaccaaag tagaagccag
     4501 gcctcccttt ggttttcttg aagaagtccc cgttagccgc agcctggaca tctgctccat
     4561 tctccaccaa gagggtcacc agcgtcatgt tccgccgttc aatggcaatg tgcagtgctg
     4621 tctggccctt gtagtagctg tctgtgtagc tggcattgac aaactgcttc aggctgtctg
     4681 tcttccgggc aacgtccagg agcagagcga tggtgtcatt ctgcccattg tgcagattga
     4741 gcatggcttt tagcagacag gtctttcctg tctctgggtc tttgaactcg ctgtcagtca
     4801 ggcgcttctt gctcctctgc aggaagggca gcaggctctc cagctcctgg cagttactct
     4861 gagccacagc atcgaagatg ctcctgcgat catagagcct cgggggcttc tcaccagcgg
     4921 agacggagtc ctgggatgac ggcctgacac tggcaggtcc atccccaggc ctctggatag
     4981 ttagaacaga gctgacagtg atgatagggc aggaagccag cccgccttcc tcataagggc
     5041 agtccagggg agaggcctcc tccgagtcac ccttcccaaa aagccgggta cgacccctgg
     5101 tagtgaagat gtggggcttg actggaggtg gcttgcagtt agggtctctg tctggagggt
     5161 ccaggcagga gttctcttgg ggtggggact cagactcctc tgagtctaag ctagcccgtt
     5221 gttccatcct agggtagtct gggacgtcat atggatatga ggagacggtg acctgggtcc
     5281 cctggcccca gtactcaaag cccacattga cattcgagta atacacggcg gtgtcctcag
     5341 gtttcaggct gttcatttgc agatacaccg tattcctggc gtcgtctctg gagatggtga
     5401 atcggccctt cacggagtct tcataacttg aacgatcacc agcactactc atacccgcga
     5461 cccactcgcg ctccttccct ggagcctggc gataccacct catactatag cgattgacgg
     5521 ggaatccaga ggctgcacag gagagtctca gagacccccc cggctgcacc aaggctcccc
     5581 cagactccac cagctgcaca tcggccatgg tggcactagt ccggtgaagg gggcggccgc
     5641 ggagcctgct tttttgtaca aacttgggtc gaattcgccc ttcgagggac ctaataactt
     5701 cgtatagcat acattatacg aagttatatt aagggttccg gggtacgcta gcggatctga
     5761 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     5821 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     5881 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     5941 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     6001 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     6061 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     6121 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     6181 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     6241 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     6301 ctatgaacta atgaccccgt aattgattac tattaatgaa ttccctggtg ctgcaagggc
     6361 gaattcggag ccaacttttc tatacaaagt tgatatcact agcgttaacg tcgacatcga
     6421 taccgtcgac ctcgagttta aacggcgcgc cggtaccgga tctccctaga caaatattac
     6481 gcgctatgag taacacaaaa ttattcagat ttcacttcct cttattcagt tttcccgcga
     6541 aaatggccaa atcttactcg gttacgccca aatttactac aacatccgcc taaaaccgcg
     6601 cgaaaattgt cacttcctgt gtacaccggc gcacaccaaa aacgtcactt ttgccacatc
     6661 cgtcgcttac atgtgttccg ccacacttgc aacatcacac ttccgccaca ctactacgtc
     6721 acccgccccg ttcccacgcc ccgcgccacg tcacaaactc caccccctca ttatcatatt
     6781 ggcttcaatc caaaataagg tatattattg atgatgctag cttaattaat tgaagacgaa
     6841 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6901 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6961 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7021 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7081 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7141 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7201 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7261 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7321 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7381 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7441 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7501 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7561 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     7621 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7681 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7741 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7801 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7861 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7921 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7981 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8041 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8101 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8161 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     8221 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8281 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8341 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8401 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8461 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8521 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8581 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8641 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8701 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
     8761 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
     8821 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
     8881 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
     8941 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
     9001 cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga
     9061 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt
     9121 tgaaggctct caagggcatc ggtcgaggac aggcctctca agtctgtata cggggacacg
     9181 gacagccttt tcgtcaccga gcgtggacac cggctcatgg aaaccagagg taagaaacgc
     9241 atcaaaaagc atgggggaaa cctggttttt gaccccgaac ggccagagct cacctggctc
     9301 gtggaatgcg agaccgtctg cggggcctgc ggcgcggatg cctactcccc ggaatcggta
     9361 tttctcgcgc ccaagctcta cgccctcaaa agtctgcact gcccctcgtg cggcgcctcc
     9421 tccaagggca agctgcgcgc caagggccac gccgcggagg ggctggacta tgacaccatg
     9481 gtcaaatgct acctggccga cgcgcagggc gaagaccggc agcgcttcag caccagcagg
     9541 accagcctca agcgcaccct ggccagcgcg cagcccggag cgcacccctt caccgtgacc
     9601 cagactacgc tgacgaggac cctgcgcccg tggaaagaca tgaccctggc ccgtctggac
     9661 gagcaccgac tactgccgta cagcgaaagc cgccccaacc cgcgaaacga ggagatatgc
     9721 tggatcgaga tgccgtagag caggtgaccg agctgtggga ccgcctggaa ctgcttggtc
     9781 aaacgctcaa aagcatgcct acggcggacg gtctcaaacc gttgaaaaac tttgcttcct
     9841 tgcaagaact gctatcgctg ggcggcgagc gccttctggc ggatttggtc agggaaaaca
     9901 tgcgagtcag ggacatgctt aacgaagtgg cccccctgct cagggatgac ggcagctgca
     9961 gctctcttaa ctaccagttg cagccggtaa taggtgtgat ttacgggccc accggctgcg
    10021 gtaagtcgca gctgctcagg aacctgcttt cttcccagct gatctcccct accccggaaa
    10081 ccgttttctt catcgccccg caggtagaca tgatcccccc atctgaactc aaagcgtggg
    10141 aaatgcaaat ctgtgagggt aactacgccc ctgggccgga tggaaccatt ataccgcagt
    10201 ctggcaccct ccgcccgcgc tttgtaaaaa tggcctatga cgatctcatc ctggaacaca
    10261 actatgacgt tagtgatccc agaaatatct tcgcccaggc cgccgcccgt gggcccattg
    10321 ccatcattat ggacgaatgc atggaaaatc ttggaggtca caagggcgtc tccaagttct
    10381 tccacgcatt tccttctaag ctacatgaca aatttcccaa gtgcaccgga tacactgtgc
    10441 tggtggttct gcacaacatg aatccccgga gggatatggc tgggaacata gccaacctaa
    10501 aaatacagtc caagatgcat ctcatatccc cacgtatgca cccatcccag cttaaccgct
    10561 ttgtaaacac ttacaccaag ggcctgcccc tggcaatcag cttgctactg aaagacattt
    10621 ttaggcacca cgcccagcgc tcctgctacg actggatcat ctacaacacc accccgcagc
    10681 atgaagctct gcagtggtgc tacctccacc ccagagacgg gcttatgccc atgtatctga
    10741 acatccagag tcacctttac cacgtcctgg aaaaaataca caggaccctc aacgaccgag
    10801 accgctggtc ccgggcctac cgcgcgcgca aaacccctaa ataaagacag caagacactt
    10861 gcttgatcca aatccaaaca gagtctggtt ttttatttat gttttaaacc gcattgggag
    10921 gggaggaagc cttcagggca gaaacctgct ggcgcagatc caacagctgc tgagaaacga
    10981 cattaagttc ccgggtcaaa gaatccaatt gtgccaaaag agccgtcaac ttgtcatcgc
    11041 gggcggatga acgggaagct gcactgcttg caagcgggct caggaaagca aagtcagtca
    11101 caatcccgcg ggcggtggct gcagcggctg aagcggcggc ggaggctgca gtctccaacg
    11161 gcgttccaga cacggtctcg taggtcaagg tagtagagtt tgcgggcagg acggggcgac
    11221 catcaatgct ggagcccatc acattctgac gcaccccggc ccatgggggc atgcgcgttg
    11281 tcaaatatga gctcacaatg cttccatcaa acgagttggt gctcatggcg gcggcggctg
//
