LOCUS       62056_22640        8938 bp DNA     circular SYN 02-NOV-2019
DEFINITION  Expression vector pVMG47_truncated-L, complete sequence.
ACCESSION   MN551092
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8938)
  AUTHORS   Lopez del Amo V Sr., Bishop AL Sr., Sanchez C HM, Bennett JB, Feng 
            X, Marshall JM, Bier E, Gantz VM.
  TITLE     Trans-complementing split-gene drive system provides flexible 
            application for safer laboratory investigation and potential field 
            deployment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8938)
  AUTHORS   Lopez del Amo V Sr., Bishop AL Sr., Sanchez C HM, Bennett JB, Feng 
            X, Marshall JM, Bier E, Gantz VM.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2019) Biological Sciences, University of 
            California San Diego, 9500 Gilman Dr, Bonner Hall 4305, La Jolla, CA
            92093, USA
REFERENCE   3  (bases 1 to 8938)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-OCT-2019) Biological Sciences, University of California San 
            Diego, 9500 Gilman Dr, Bonner Hall 4305, La Jolla, CA 92093, USA"
FEATURES             Location/Qualifiers
     source          1..8938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    877..898
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        913..943
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1065..2072
                     /label=white homology arm truncated
                     /note="white homology arm truncated"
     promoter        2078..2874
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1
                     snRNA (Port et al., 2014)"
     misc_feature    2874..2893
                     /label=w2 gRNA
                     /note="w2 gRNA"
     misc_RNA        2894..2969
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2970..3390
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3
                     snRNA (Port et al., 2014)"
     misc_feature    3390..3409
                     /label=y1 gRNA
                     /note="y1 gRNA"
     misc_feature    3410..3485
                     /label=gRNA scaffold
                     /note="gRNA scaffold"
     misc_feature    3492..3793
                     /label=U6-3_3'UTR
                     /note="U6-3_3'UTR"
     polyA_signal    complement(3820..3941)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4067..4783)
                     /label=EGFP
                     /note="enhanced GFP"
     regulatory      4838..4962
                     /label=Hsp70 promoter
                     /note="Hsp70 promoter"
                     /regulatory_class="promoter"
     misc_feature    4994..5037
                     /label=3xP3 eye promoter
                     /note="3xP3 eye promoter"
     misc_feature    5060..6071
                     /label=white homology arm
                     /note="white homology arm"
     promoter        complement(6142..6160)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6167..6183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6324..6752
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             7096..7887
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             7908..8765
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg gtaccgagct
     1021 cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttgctcaa agtccccaaa
     1081 cgaaagccaa agatgccagc gatttacact cgcaattgtc ttatgaggtg gcaacatggt
     1141 ggaggaacct tgtcactccc accacccatc gtcccatcgt cccatcgtcc atcgcccaac
     1201 gacctgtgac cccggccccg tcaaccgtcc accgcccagc gcccactttc aaggccatcc
     1261 accaagtgcc agccctaatt tgcacatttt aaataggcct cgccatctct ttttttggga
     1321 aacttggaaa actcggatct tgggcatttt tattaaacag atgggctcct tctttataaa
     1381 gccgaggggg tggggtgggg gttagtgccc ggaaacaggg acggcggagc agggattagc
     1441 caggctgggc tagatttatg cacagacgcc ttcattttta gggacgaacg ccgtgaaatt
     1501 gaaaagtttt cagcttgaaa ccttttctag atgcacaaaa aataaataaa agtataaacc
     1561 tacttcgtag gatacttcgt tttgttcggg gttagatgag cataacgctt gtagttgata
     1621 tttgagatcc cctatcattg cagggtgaca gcggagcggc ttcgcagagc tgcattaacc
     1681 agggcttcgg gcaggccaaa aactacggca cgctcctgcc acccagtccg ccggaggact
     1741 ccggttcagg gagcggccaa ctagccgaga acctcaccta tgcctggcac aatatggaca
     1801 tctttggggc ggtcaatcag ccgggctccg gatggcggca gctggtcaac cggacacgcg
     1861 gactattctg caacgagcga cacataccgg cgcccaggaa acatttgctc aagaacggtg
     1921 agtttctatt cgcagtcggc tgatctgtgt gaaatcttaa taaagggtcc aattaccaat
     1981 ttgaaactca gtttgcggcg tggcctatcc gggcgaactt ttggccgtga tgggcagttc
     2041 cggtgccgga aagacgaccc tgctgaatgc ccacgtcatt ttcaacgtcc tcgatagtat
     2101 agtggttagt atccccgcct gtcacgcggg agaccggggt tcaattcccc gtcggggaga
     2161 atctgtgatt cttttttttt ttcttttact ttgttatata aacaattttt gttttaattg
     2221 aatctaattt gccattgctt ttaggaatct caggcatcca gcaagcgttt gtccgccgaa
     2281 tcgcccatca gtgaagaaga tcctgtggcg gctacgaaaa tctccccggc catgtcggcc
     2341 tccacctcca gcgaaaaacc catcagcgag ctggccacct ctgtgctgac ccaccgcttt
     2401 ccagactcca cctcctcacc cggcgaacat ggccttggac gaatgcagtt gtcgatccgc
     2461 tacagcgccc agcgtcaaaa actagacgtg accatacaca aaatccagaa gataccactt
     2521 cgcgatccca gcaatatccc cgatccgtat gttaagctgt atctgttgcc tggacgcacc
     2581 aaggagtcga aacgcaagac gagcgtgatc aaggacaact gcaaccccgt ctacgatgca
     2641 tcctttgagt acctgatttc cattgccgaa ctcaggcaga cggaactgga ggtgacggtg
     2701 tgcacccaaa agggattcct atccggcggt agtcccatca ttggcatggt aggtacccga
     2761 aagcaacccc ttagttacag acacagcgcg tacgtccttc gcatccttat gattcccaag
     2821 tacatattct gcaagagtac agtatatata ggaaagatat ccgggtgaac ttcggcgata
     2881 cttggatgcc ctggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     2941 aacttgaaaa agtggcaccg agtcggtgct tttttgctca cctgtgattg ctcctactca
     3001 aatacaaaaa catcaaattt tctgtcaata aagcatattt atttatattt attttacagg
     3061 aaagaattcc ttttaaagtg tattttaacc tataatgaaa aacgattaaa aaaaatacat
     3121 aaaataattc gaaaattttt gaatagccca ggttgataaa aattcatttc atacgtttta
     3181 taacttatgc ccctaagtat tttttgacca tagtgtttca attctacatt aattttacag
     3241 agtagaatga aacgccacct actcagccaa gaggcgaaaa ggttagctcg ccaagcagag
     3301 agggcgccag tgctcactac tttttataat tctcaacttc tttttccaga ctcagttcgt
     3361 atatatagac ctattttcaa tttaacgtcg gttttggaca ctggaaccgg ttttagagct
     3421 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     3481 ggtgcttttt tgcctacctg gagcctgaga gttgttcaat aaaataaaaa tgtttcgttt
     3541 ttttgctttc gccagtattt attatttttc atcaatatgt attcaatttg gtatgtattt
     3601 agtaattgta atatatagac aatggttttc cgttgacgta catacatctg acgtgtgttt
     3661 atttagacat aatagttatg ttttcacatc tttttaatgt tcgcttaatg cgtatgcatt
     3721 ctagacaatt gtgctcggca acagtatatt tgtggtgtgc caaccaacaa cctgcaggag
     3781 ctccagcttt tgtgctagcg tacgcgtatc gataagcttt aagatacatt gatgagtttg
     3841 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     3901 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     3961 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     4021 acaaatgtgg tatggctgat tatgatctag agtcgcggcc gctttacttg tacagctcgt
     4081 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     4141 tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca
     4201 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     4261 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     4321 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     4381 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     4441 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     4501 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     4561 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     4621 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     4681 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     4741 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtggat
     4801 cccgggcccg cggtaccgtc gactctagcg gtaccccgat tgtttagctt gttcagctgc
     4861 gcttgtttat ttgcttagct ttcgcttagc gacgtgttca ctttgcttgt ttgaattgaa
     4921 ttgtcgctcc gtagacgaag cgcctctatt tatactccgg cggtcgaggg ttcgaaatcg
     4981 ataagcttgg atcctaattg aattagctct aattgaatta gtctctaatt gaattagatc
     5041 cccgggcgag ctcgcatatg gcatccaagt atcgccatcc gggatgcgac tgctcaatgg
     5101 ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc tatgtccagc aggatgacct
     5161 ctttatcggc tccctaacgg ccagggaaca cctgattttc caggccatgg tgcggatgcc
     5221 acgacatctg acctatcggc agcgagtggc ccgcgtggat caggtgatcc aggagctttc
     5281 gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg
     5341 cggagaaagg aagcgtctgg cattcgcctc cgaggcacta accgatccgc cgcttctgat
     5401 ctgcgatgag cccacctccg gactggactc atttaccgcc cacagcgtcg tccaggtgct
     5461 gaagaagctg tcgcagaagg gcaagaccgt catcctgacc attcatcagc cgtcttccga
     5521 gctgtttgag ctctttgaca agatccttct gatggccgag ggcagggtag ctttcttggg
     5581 cactcccagc gaagccgtcg acttcttttc ctagtgagtt cgatgtgttt attaagggta
     5641 tctagcatta cattacatct caactcctat ccagcgtggg tgcccagtgt cctaccaact
     5701 acaatccggc ggacttttac gtacaggtgt tggccgttgt gcccggacgg gagatcgagt
     5761 cccgtgatcg gatcgccaag atatgcgaca attttgccat tagcaaagta gcccgggata
     5821 tggagcagtt gttggccacc aaaaatttgg agaagccact ggagcagccg gagaatgggt
     5881 acacctacaa ggccacctgg ttcatgcagt tccgggcggt cctgtggcga tcctggctgt
     5941 cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt
     6001 ccagtggaaa caaatgatat aacgcttaca attcttggaa acaaattcgc tagattttag
     6061 atagaattgc caagggcgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg
     6121 catctagagg gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
     6181 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     6241 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6301 tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6361 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6421 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6481 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6541 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6601 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6661 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6721 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa attcagggcg caagggctgc
     6781 taaaggaagc ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     6841 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct
     6901 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg
     6961 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
     7021 gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga
     7081 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     7141 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     7201 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     7261 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     7321 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     7381 gaagtgccgg ggcaggatct cctgtcatcc caccttgctc ctgccgagaa agtatccatc
     7441 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     7501 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     7561 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     7621 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     7681 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     7741 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     7801 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     7861 ttctatcgcc ttcttgacga gttcttctga attgaaaaag gaagagtatg agtattcaac
     7921 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7981 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8041 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8101 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8161 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8221 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8281 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8341 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8401 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8461 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8521 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8581 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8641 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8701 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8761 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8821 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8881 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
