LOCUS       62056_22590       10524 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1642..2454
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(2789..3183)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            3525..3612
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             4851..5819
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6333..7169
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7159..8007
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8290..9369)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(9370..9447)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        9677..9705
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     misc_feature    9746..9802
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
ORIGIN
        1 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg
       61 tgccggatca agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
      121 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
      181 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
      241 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
      301 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
      361 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
      421 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
      481 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
      541 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
      601 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
      661 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
      721 attcatcgac tgtggccggc tgggtgtggc ggtaccgcta tcaggacata gcgttggcta
      781 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
      841 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
      901 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
      961 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     1021 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccgggct
     1081 cgatcccctc gcgagttggt tcagctgctg cctgaggctg gacgacctcg cggagttcta
     1141 ccggcagtgc aaatccgtcg gcatccagga aaccagcagc ggctatccgc gcatccatgc
     1201 ccccgaactg caggagtggg gaggcacgat ggccgctttg gtcgacggat cagtgagggt
     1261 ttgcaactgc gggtcaagga tctggatttc gatcacggca cgatcatcgt gcgggagggc
     1321 aagggctcca aggatcgggc cttgatgtta cccgagagct tggcacccag cctgcgcgag
     1381 caggggaatt gatccggtgg atgacctttt gaatgacctt taatagatta tattactaat
     1441 taattgggga ccctagaggt cccctttttt attttaaaaa ttttttcaca gaacggttta
     1501 caagcataaa gctacacgtg gccaatatgg actacttcat cgcccccgtt ttcaccatgg
     1561 tcaaatatta tacgcaaggc gactaggtgc agatgccgct ggctaatcag gttcatcatg
     1621 ccgtttgtga tggcttccat gatgagccat attcaacggg aaacgtcttg ctctaggccg
     1681 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1741 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt
     1801 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1861 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1921 gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat
     1981 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     2041 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     2101 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     2161 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     2221 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     2281 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     2341 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     2401 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaattt
     2461 ttttaaggca gttattgtct tcaaattccc gttgcacata gcccggcaat tcctttccct
     2521 gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga tagagaaggg
     2581 tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg gccgtcctgg
     2641 ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca gtctatcgct
     2701 tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca ttgccagcca
     2761 gtgcccgtca ctcccgtact aactgtcacg aacccctgca ataactgtca cgcccccctg
     2821 caataactgt cacgaacccc tgcaataact gtcacgcccc caaacctgca aacccagcag
     2881 gggcgggggc tggcggggtg ttggaaaaat ccatccatga ttatctaaga ataatccact
     2941 aggcgcggtt atcagcgccc ttgtggggcg ctgctgccct tgcccaatat gcccggccag
     3001 aggccggata gctggtctat tcgctgcgct aggctacaca ccgccccacc gctgcgcggc
     3061 agggggaaag gcgggcaaag cccgctaaac cccacaccaa accccgcaga aatacgctgg
     3121 agcgctttta gccgctttag cggcctttcc ccctacccga agggtggggg cgcgtgtgca
     3181 gccccgcagg gcctgtctcg gtcgatcatt cagcccggct catccttctg gcgtggcggc
     3241 agaccgaaca aggcgcggtc gtggtcgcgt tcaaggtacg catccattgc cgccatgagc
     3301 cgatcctccg gccactcgct gctgttcacc ttggccaaaa tcatggcccc caccagcacc
     3361 ttgcgccttg tttcgttctt gcgctcttgc tgctgttccc ttgcccgctc ccgctgaatt
     3421 tcggcattga ttcgcgctcg ttgttcttcg agcttggcca gccgatccgc cgccttgttg
     3481 ctccccttaa ccatcttgac accccattgt taatgtgctg tctcgtaggc tatcatggag
     3541 gcacagcggc ggcaatcccg accctacttt gtaggggagg gcgcacttac cggtttctct
     3601 tcgagaaact ggcctaacgg ccacccttcg ggcggtgcgc tctccgaggg ccattgcatg
     3661 gagccgaaaa gcaaaagcaa cagcgaggca gcatggcgat ttatcacctt acggcgaaaa
     3721 ccggcagcag gtcgggcggc caatcggcca gggccaaggc cgactacatc cagcgcgaag
     3781 gcaagtatgc ccgcgacatg gatgaagtct tgcacgccga atccgggcac atgccggagt
     3841 tcgtcgagcg gcccgccgac tactgggatg ctgccgacct gtatgaacgc gccaatgggc
     3901 ggctgttcaa ggaggtcgaa tttgccctgc cggtcgagct gaccctcgac cagcagaagg
     3961 cgctggcgtc cgagttcgcc cagcacctga ccggtgccga gcgcctgccg tatacgctgg
     4021 ccatccatgc cggtggcggc gagaacccgc actgccacct gatgatctcc gagcggatca
     4081 atgacggcat cgagcggccc gccgctcagt ggttcaagcg gtacaacggc aagaccccgg
     4141 agaagggcgg ggcacagaag accgaagcgc tcaagcccaa ggcatggctt gagcagaccc
     4201 gcgaggcatg ggccgaccat gccaaccggg cattagagcg ggctggccac gacgcccgca
     4261 ttgaccacag aacacttgag gcgcagggca tcgagcgcct gcccggtgtt cacctggggc
     4321 cgaacgtggt ggagatggaa ggccggggca tccgcaccga ccgggcagac gtggccctga
     4381 acatcgacac cgccaacgcc cagatcatcg acttacagga ataccgggag gcaatagacc
     4441 atgaacgcaa tcgacagagt gaagaaatcc agaggcatca acgagttagc ggagcagatc
     4501 gaaccgctgg cccagagcat ggcgacactg gccgacgaag cccggcaggt catgagccag
     4561 accaagcagg ccagcgaggc gcaggcggcg gagtggctga aagcccagcg ccagacaggg
     4621 gcggcatggg tggagctggc caaagagttg cgggaggtag ccgccgaggt gagcagcgcc
     4681 gcgcagagcg cccggagcgc gtcgcggggg tggcactgga agctatggct aaccgtgatg
     4741 ctggcttcca tgatgcctac ggtggtgctg ctgatcgcat cgttgctctt gctcgacctg
     4801 acgccactga caaccgagga cggctcgatc tggctgcgct tggtggcccg atgaagaacg
     4861 acaggacttt gcaggccata ggccgacagc tcaaggccat gggctgtgag cgcttcgata
     4921 tcggcgtcag ggacgcaccc accggccaga tgatgaaccg ggaatggtca gccgccgaag
     4981 tgctccagaa cacgccatgg ctcaagcgga tgaatgccca gggcaatgac gtgtatatca
     5041 ggcccgccga gcaggagcgg catggtctgg tgctggtgga cgacctcagc gagtttgacc
     5101 tggatgacat gaaagccgag ggccgggagc ctgccctggt agtggaaacc agcccgaaga
     5161 actatcaggc atgggtcaag gtggccgacg ccgcaggcgg tgaacttcgg gggcagattg
     5221 cccggacgct ggccagcgag tacgacgccg acccggccag cgccgacagc cgccactatg
     5281 gccgcttggc gggcttcacc aaccgcaagg acaagcacac cacccgcgcc ggttatcagc
     5341 cgtgggtgct gctgcgtgaa tccaagggca agaccgccac cgctggcccg gcgctggtgc
     5401 agcaggctgg ccagcagatc gagcaggccc agcggcagca ggagaaggcc cgcaggctgg
     5461 ccagcctcga actgcccgag cggcagctta gccgccaccg gcgcacggcg ctggacgagt
     5521 accgcagcga gatggccggg ctggtcaagc gcttcggtca tgacctcagc aagtgcgact
     5581 ttatcgccgc gcagaagctg gccagccggg gccgcagtgc cgaggaaatc ggcaaggcca
     5641 tggccgaggc cagcccagcg ctggcagagc gcaagcccgg ccacgaagcg gattacatcg
     5701 agcgcaccgt cagcaaggtc atgggtctgc ccagcgtcca gcttgcgcgg gccgagctgg
     5761 cacgggcacc ggcaccccgc cagcgaggca tggacagggg cgggccagat ttcagcatgt
     5821 agtgcttgcg ttggtactca cgcctgttat actatgagta ctcacgcaca gaagggggtt
     5881 ttatggaata cgaaaaaagc gcttcagggt cggtctacct gatcaaaagt gacaagggct
     5941 attggttgcc cggtggcttt ggttatacgt caaacaaggc cgaggctggc cgcttttcag
     6001 tcgctgatat ggccagcctt aaccttgacg gctgcacctt gtccttgttc cgcgaagaca
     6061 agcctttcgg ccccggcaag tttctcggtg actgatatga aagaccaaaa ggacaagcag
     6121 accggcgacc tgctggccag ccctgacgct gtacgccaag cgcgatatgc cgagcgcatg
     6181 aaggccaaag ggatgcgtca gcgcaagttc tggctgaccg acgacgaata cgaggcgctg
     6241 cgcgagtgcc tggaagaact cagagcggcg cagggcgggg gtagtgaccc cgccagcgcc
     6301 taaccaccaa ctgcctgcaa aggaggcaat caatggctac ccataagcct atcaatattc
     6361 tggaggcgtt cgcagcagcg ccgccaccgc tggactacgt tttgcccaac atggtggccg
     6421 gtacggtcgg ggcgctggtg tcgcccggtg gtgccggtaa atccatgctg gccctgcaac
     6481 tggccgcaca gattgcaggc gggccggatc tgctggaggt gggcgaactg cccaccggcc
     6541 cggtgatcta cctgcccgcc gaagacccgc ccaccgccat tcatcaccgc ctgcacgccc
     6601 ttggggcgca cctcagcgcc gaggaacggc aagccgtggc tgacggcctg ctgatccagc
     6661 cgctgatcgg cagcctgccc aacatcatgg ccccggagtg gttcgacggc ctcaagcgcg
     6721 ccgccgaggg ccgccgcctg atggtgctgg acacgctgcg ccggttccac atcgaggaag
     6781 aaaacgccag cggccccatg gcccaggtca tcggtcgcat ggaggccatc gccgccgata
     6841 ccgggtgctc tatcgtgttc ctgcaccatg ccagcaaggg cgcggccatg atgggcgcag
     6901 gcgaccagca gcaggccagc cggggcagct cggtactggt cgataacatc cgctggcagt
     6961 cctacctgtc gagcatgacc agcgccgagg ccgaggaatg gggtgtggac gacgaccagc
     7021 gccggttctt cgtccgcttc ggtgtgagca aggccaacta tggcgcaccg ttcgctgatc
     7081 ggtggttcag gcggcatgac ggcggggtgc tcaagcccgc cgtgctggag aggcagcgca
     7141 agagcaaggg ggtgccccgt ggtgaagcct aagaacaagc acagcctcag ccacgtccgg
     7201 cacgacccgg cgcactgtct ggcccccggc ctgttccgtg ccctcaagcg gggcgagcgc
     7261 aagcgcagca agctggacgt gacgtatgac tacggcgacg gcaagcggat cgagttcagc
     7321 ggcccggagc cgctgggcgc tgatgatctg cgcatcctgc aagggctggt ggccatggct
     7381 gggcctaatg gcctagtgct tggcccggaa cccaagaccg aaggcggacg gcagctccgg
     7441 ctgttcctgg aacccaagtg ggaggccgtc accgctgaat gccatgtggt caaaggtagc
     7501 tatcgggcgc tggcaaagga aatcggggca gaggtcgata gtggtggggc gctcaagcac
     7561 atacaggact gcatcgagcg cctttggaag gtatccatca tcgcccagaa tggccgcaag
     7621 cggcaggggt ttcggctgct gtcggagtac gccagcgacg aggcggacgg gcgcctgtac
     7681 gtggccctga accccttgat cgcgcaggcc gtcatgggtg gcggccagca tgtgcgcatc
     7741 agcatggacg aggtgcgggc gctggacagc gaaaccgccc gcctgctgca ccagcggctg
     7801 tgtggctgga tcgaccccgg caaaaccggc aaggcttcca tagatacctt gtgcggctat
     7861 gtctggccgt cagaggccag tggttcgacc atgcgcaagc gccgcaagcg ggtgcgcgag
     7921 gcgttgccgg agctggtcgc gctgggctgg acggtaaccg agttcgcggc gggcaagtac
     7981 gacatcaccc ggcccaaggc ggcaggctga ccccccccac tctattgtaa acaagacatt
     8041 tttatctttt atattcaatg gcttattttc ctgctaattg gtaataccat gaaaaatacc
     8101 atgctcagaa aaggcttaac aatattttga aaaattgcct actgagcgct gccgcacagc
     8161 tccataggcc gctttcctgg ctttgcttcc agatgtatgc tcttctgctc ccgaacgcca
     8221 gcaagacgta gcccagcgcg tcggccagct tgcaattcgc gctaacttac attaattgcg
     8281 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8341 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac
     8401 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
     8461 gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg
     8521 gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac
     8581 gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac
     8641 cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga
     8701 catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata
     8761 tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag
     8821 cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc
     8881 atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg
     8941 aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat
     9001 gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac
     9061 gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt
     9121 aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat
     9181 cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc
     9241 cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac
     9301 cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac
     9361 tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg
     9421 aaaggttttg caccattcga tggtgtcaac gtaaatgccg cttcgccttc gcgcgcgaat
     9481 tgcaagctga tccgggctta tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc
     9541 catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca
     9601 aggcgcactc ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat
     9661 attctgaaat gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc
     9721 ggataacaat ttcacacagg aaacagaatt cgagctcggt acccggggat cctctagagt
     9781 cgacctgcag gcatgcaagc ttggctgttt tggcggatga gagaagaagc tttatgcttg
     9841 taaaccgttt tgtgaaaaaa tttttaaaat aaaaaagggg acctctaggg tccccaatta
     9901 attagtaata taatctatta aaggtcattc aaaaggtcat ccaccggatc agcttagtaa
     9961 agccctcgct agattttaat gcggatgttg cgattacttc gccaactatt gcgataacaa
    10021 gaaaaagcca gcctttcatg atatatctcc caatttgtgt agggcttatt atgcacgctt
    10081 aaaaataata aaagcagact tgacctgata gtttggctgt gagcaattat gtgcttagtg
    10141 catctaacgc ttgagttaag ccgcgccgcg aagcggcgtc ggcttgaacg aattgttaga
    10201 cattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac
    10261 tgatctgcgc gagcttcacg ctgccgcaag cactcagggc gcaagggctg ctaaaggaag
    10321 cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac
    10381 tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg
    10441 cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca
    10501 gctggggcgc cctctggtaa ggtt
//
