LOCUS       40924_46023        6607 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVLG6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Zeigler DR.
  TITLE     Sequence of pVLG6, a shuttle vector for constructing GFP fusions in 
            Bacillus megaterium and other Gram-positive bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Zeigler DR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2011) Bacillus Genetic Stock Center, The Ohio 
            State University, 484 W 12th Ave, Columbus, OH 43210, USA
REFERENCE   3  (bases 1 to 6607)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6607)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-APR-2011) Bacillus Genetic Stock Center, The Ohio State 
            University, 484 W 12th Ave, Columbus, OH 43210, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..34
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             61..774
                     /label=GFP
                     /note="green fluorescent protein"
     primer_bind     complement(792..808)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1202..1387
                     /codon_start=1
                     /gene="cop"
                     /product="Cop"
                     /label=cop
                     /note="copy control regulatory protein for Gram-positive 
                     replication"
                     /protein_id="AEP13839.1"
                     /translation="MLGGTVMVVDRKEEKKVAVTLRLTTEENEILNRIKEKYNISKSDA
                     TGILIKKYAKEEYGAF"
     gene            1202..1387
                     /gene="cop"
                     /label=cop
     CDS             1468..2067
                     /codon_start=1
                     /gene="repF"
                     /product="RepF"
                     /label=repF
                     /note="initiation protein for Gram-positive replication"
                     /protein_id="AEP13838.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEGRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     gene            1468..2067
                     /gene="repF"
                     /label=repF
     promoter        complement(2703..2721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2728..2744)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(2885..3340)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3367..3471
                     /label=AmpR promoter
     CDS             3472..4329
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4503..5091
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5458..6105)
                     /gene="cat"
                     /label=cat
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     protein_bind    6443..6464
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6479..6509
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6517..6533
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6541..6557
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6578..6596
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
       61 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      121 gacgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      181 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      241 gtcactactc tgacttatgg tgttcaatgc ttttcaagat acccagatca tatgaaacag
      301 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      361 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      421 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      481 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      541 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      601 cattatcaac aaaatactcc aattggcgat ggccctgtcc tttcaccaga caaccattac
      661 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      721 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataaatg
      781 tccagacttc agatccacta gttctagagc ggccgccacc gcggacgatt tagagatgta
      841 gaggtacttt tatgccgaga aaactttttg cgtgtgacag tccttaaaat atacttagag
      901 cgtaagcgaa agtagtagcg acagctatta actttcggtt gcaaagctct aggattttta
      961 atggacgcag cgcatcacac gcaaaaagga aattggaata aatgcgaaat ttgagatgtt
     1021 aattaaagac ctttttgagg tctttttttc ttagattttt ggggttattt aggggagaaa
     1081 acataggggg gtactacgac ctccccccct aggtgtccat tgtccattgt ccaaacaaat
     1141 aaataaatat tgggttttta atgttaaaag gttgtttttt atgttaaagt gaaaaaaaca
     1201 gatgttggga ggtacagtga tggttgtaga tagaaaagaa gagaaaaaag ttgctgttac
     1261 tttaagactt acaacagaag aaaatgagat attaaataga atcaaagaaa aatataatat
     1321 tagcaaatca gatgcaaccg gtattctaat aaaaaaatat gcaaaggagg aatacggtgc
     1381 attttaaaca aaaaaagata gacagcactg gcatgctgcc tatctatgac taaattttgt
     1441 taagtgtatt agcaccgtta ttatatcatg agcgaaaatg taataaaaga aactgagaac
     1501 aagaaaaatt caagaggacg taattggaca tttgttttat atccagaatc agcaaaagcc
     1561 gagtggttag agtatttaaa agagttacac attcaatttg tagtgtctcc attacatgat
     1621 agggatactg atacagaagg taggatgaaa aaagagcatt atcatattct agtgatgtat
     1681 gagggtaata aatcttatga acagataaaa ataattacag aagaattgaa tgcgactatt
     1741 ccgcagattg caggaagtgt gaaaggtctt gtgagatata tgcttcacat ggacgatcct
     1801 aataaattta aatatcaaaa agaagatatg atagtttatg gcggtgtaga tgttgatgaa
     1861 ttattaaaga aaacaacaac agatagatat aaattaatta aagaaatgat tgagtttatt
     1921 gatgaacaag gaatcgtaga atttaagagt ttaatggatt atgcaatgaa gtttaaattt
     1981 gatgattggt tcccgctttt atgtgataac tcggcgtatg ttattcaaga atatataaaa
     2041 tcaaatcggt ataaatctga ccgatagatt ttgaatttaa gagtgtcaca agacactctt
     2101 ttttcgcacc agcgaaaact ggtttaagcc gactgcgcaa aagacataat cgattcacaa
     2161 aaaataggca cacgaaaaac aagttaaggg atgcagttta tgcatccctt aacttactta
     2221 ttaaataatt tatagctatt gaaaagagat aagaattgtt caaagctaat attgtttaaa
     2281 tcgtcaattc ctgcatgttt taaggaattg ttaaattgat tttttgtaaa tattttcttg
     2341 tattctttgt taacccattt cataacgaaa taattatact tttgtttatc tttgtgtgat
     2401 attcttgatt tttttctact taatctgata agtgagctat tcactttagg tttaggatga
     2461 aaatattctc ttggaaccat acttaatata gaaatatcaa cttctgccat taaaagtaat
     2521 gccaatgagc gttttgtatt taataatctt ttagcaaacc cgtattccac gattaaataa
     2581 atctcattag ctatactatc aaaaacaatt ttgcgtatta tatccgtact tatgttataa
     2641 ggtatattac catatatttt ataggattgg tttttaggaa ccgcggtgga gctccaattc
     2701 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
     2761 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     2821 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     2881 atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     2941 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     3001 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3061 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     3121 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3181 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3241 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3301 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     3361 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3421 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3481 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3541 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3601 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3661 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     3721 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3781 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     3841 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     3901 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     3961 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4021 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4081 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4141 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4201 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4261 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4321 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4381 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4441 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4501 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4561 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4621 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     4681 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4741 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4801 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4861 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     4921 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4981 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5041 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5101 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5161 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5221 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     5281 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagggc gaggctagtt
     5341 acccttaagt tattggtatg actggtttta agcgcaaaaa aagttgcttt ttcgtaccta
     5401 ttaatgtatc gttagaaaac cgactgtaaa aagtacagtc ggcattatct catattataa
     5461 aagccagtca ttaggcctat ctgacaattc ctgaatagag ttcataaaca atcctgcatg
     5521 ataaccatca caaacagaat gatgtacctg taaagatagc ggtaaatata ttgaattacc
     5581 tttattaatg aattttcctg ctgtaataat gggtagaagg taattactat tattattgat
     5641 atttaagtta aacccagtaa atgaagtcca tggaataata gaaagagaaa aagcattttc
     5701 aggtataggt gttttgggaa acaatttccc cgaaccatta tatttctcta catcagaaag
     5761 gtataaatca taaaactctt tgaagtcatt ctttacagga gtccaaatac cagagaatgt
     5821 tttagataca ccatcaaaaa ttgtataaag tggctctaac ttatcccaat aacctaactc
     5881 tccgtcgcta ttgtaaccag ttctaaaagc tgtatttgag tttatcaccc ttgtcactaa
     5941 gaaaataaat gcagggtaaa atttatatcc ttcttgtttt atgtttcggt ataaaacact
     6001 aatatcaatt tctgtggtta tactaaaagt cgtttgttgg ttcaaataat gattaaatat
     6061 ctcttttctc ttccaattgt ctaaatcaat tttattaaag ttcatttgat atgcctccta
     6121 aatttttatc taaagtgaat ttaggaggct tacttgtctg ctttcttcat tagaatcaat
     6181 ccttttttaa aagtcaatat tactgtaaca taaatatata ttttaaaaat atcccacttt
     6241 atccaatttt cgtttgttga actaatgggt gctttagttg aagaataaaa gaccacatta
     6301 aaaaatgtgg tcttttgtgt ttttttaaag gatttgagcg tagcgaaaaa tccttttctt
     6361 tcttatcttg ataataaggg taactattgc cctggcacga caggtttccc gactggaaag
     6421 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6481 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6541 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
     6601 aaaagct
//
