LOCUS       pVL1392.        9639 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector containing the polyhedrin promoter, for 
            use with the BD BaculoGold(TM) system. For an alternative MCS, see 
            pVL1393.
ACCESSION   .
VERSION     .
KEYWORDS    pVL1392.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label=PTP
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    4093..4095
                     /label=mutated start codon
                     /note="mutated start codon"
                     /note="the ATG start codon of polyhedrin was mutated to
                     ATT"
     misc_feature    4134..4192
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     4654..6109
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     primer_bind     complement(7020..7036)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7044..7060)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7068..7098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7113..7134)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7422..8010)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8184..9041)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9042..9146)
                     /label=AmpR promoter
     primer_bind     9620..9636
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atcagatctg
     4141 cagcggccgc tccagaattc tagaaggtac ccgggatcct ttcctgggac ccggcaagaa
     4201 ccaaaaactc actctcttca aggaaatccg taatgttaaa cccgacacga tgaagcttgt
     4261 cgttggatgg aaaggaaaag agttctacag ggaaacttgg acccgcttca tggaagacag
     4321 cttccccatt gttaacgacc aagaagtgat ggatgttttc cttgttgtca acatgcgtcc
     4381 cactagaccc aaccgttgtt acaaattcct ggcccaacac gctctgcgtt gcgaccccga
     4441 ctatgtacct catgacgtga ttaggatcgt cgagccttca tgggtgggca gcaacaacga
     4501 gtaccgcatc agcctggcta agaagggcgg cggctgccca ataatgaacc ttcactctga
     4561 gtacaccaac tcgttcgaac agttcatcga tcgtgtcatc tgggagaact tctacaagcc
     4621 catcgtttac atcggtaccg actctgctga agaggaggaa attctccttg aagtttccct
     4681 ggtgttcaaa gtaaaggagt ttgcaccaga cgcacctctg ttcactggtc cggcgtatta
     4741 aaacacgata cattgttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt
     4801 tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta
     4861 tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc
     4921 ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc
     4981 gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag
     5041 cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc
     5101 gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact
     5161 cgacgtaaac acgttaaata aagcttggac atatttaaca tcgggcgtgt tagctttatt
     5221 aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt
     5281 tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt
     5341 agttgagctt tttggaatta tttctgattg cgggcgtttt tgggcgggtt tcaatctaac
     5401 tgtgcccgat tttaattcag acaacacgtt agaaagcgat ggtgcaggcg gtggtaacat
     5461 ttcagacggc aaatctacta atggcggcgg tggtggagct gatgataaat ctaccatcgg
     5521 tggaggcgca ggcggggctg gcggcggagg cggaggcgga ggtggtggcg gtgatgcaga
     5581 cggcggttta ggctcaaatg tctctttagg caacacagtc ggcacctcaa ctattgtact
     5641 ggtttcgggc gccgtttttg gtttgaccgg tctgagacga gtgcgatttt tttcgtttct
     5701 aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag gttccgtcgg
     5761 cattggtgga gcgggcggca attcagacat cgatggtggt ggtggtggtg gaggcgctgg
     5821 aatgttaggc acgggagaag gtggtggcgg cggtgccgcc ggtataattt gttctggttt
     5881 agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc gctggctgca caacggaagg
     5941 tcgtctgctt cgaggcagcg cttggggtgg tggcaattca atattataat tggaatacaa
     6001 atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg tttaccgtgc cgatatttaa
     6061 caaccgctca atgtaagcaa ttgtattgta aagagattgt ctcaagctcc gcacgccgat
     6121 aacaagcctt ttcattttta ctacagcatt gtagtggcga gacacttcgc tgtcgtcgac
     6181 gtacatgtat gctttgttgt caaaaacgtc gttggcaagc tttaaaatat ttaaaagaac
     6241 atctctgttc agcaccactg tgttgtcgta aatgttgttt ttgataattt gcgcttccgc
     6301 agtatcgaca cgttcaaaaa attgatgcgc atcaattttg ttgttcctat tattgaataa
     6361 ataagattgt acagattcat atctacgatt cgtcatggcc accacaaatg ctacgctgca
     6421 aacgctggta caattttacg aaaactgcaa aaacgtcaaa actcggtata aaataatcaa
     6481 cgggcgcttt ggcaaaatat ctattttatc gcacaagccc actagcaaat tgtatttgca
     6541 gaaaacaatt tcggcgcaca attttaacgc tgacgaaata aaagttcacc agttaatgag
     6601 cgaccaccca aattttataa aaatctattt taatcacggt tccatcaaca accaagtgat
     6661 cgtgatggac tacattgact gtcccgattt atttgaaaca ctacaaatta aaggcgagct
     6721 ttcgtaccaa cttgttagca atattattag acagctgtgt gaagcgctca acgatttgca
     6781 caagcacaat ttcatacaca acgacataaa actcgaaaat gtcttatatt tcgaagcact
     6841 tgatcgcgtg tatgtttgcg attacggatt gtgcaaacac gaaaactcac ttagcgtgca
     6901 cgacggcacg ttggagtatt ttagtccgga aaaaattcga cacacaacta tgcacgtttc
     6961 gtttgactgg tacgcggcgt gttaacatac aagttgctaa ccggcggttc gtaatcatgg
     7021 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7081 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7141 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7201 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7261 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7321 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7381 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7441 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7501 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7561 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7621 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7681 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7741 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7801 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7861 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7921 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7981 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8041 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8101 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8161 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8221 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8281 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8341 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8401 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8461 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8521 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8581 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8641 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8701 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8761 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8821 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8881 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8941 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9001 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9061 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9121 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     9181 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     9241 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     9301 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     9361 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     9421 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     9481 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     9541 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     9601 ttttcccagt cacgacgttg taaaacgacg gccagtgcc
//
