LOCUS       40924_46003       10172 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVK9PtacMuAB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Gorshkova NV, Gak ER, Tokmakova IL, Mashko SV.
  TITLE     Genetic tools C. glutamicum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   Gorshkova NV, Gak ER, Tokmakova IL, Mashko SV.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2014) Lab3, AGRI, 1st Dorozhny Proezd, 1-1, Moscow
            117545, Russia
REFERENCE   3  (bases 1 to 10172)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10172)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-DEC-2014) Lab3, AGRI, 1st Dorozhny Proezd, 1-1, Moscow 117545, 
            Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(463..1857)
                     /codon_start=1
                     /gene="rep"
                     /product="Rep"
                     /label=rep
                     /note="replication protein of pCG1 plasmid from C.
                     glutamicum"
                     /protein_id="AKN19584.1"
                     /translation="MTLADSRDIDTPAWKFSADLFDTHPELALRSRGWTSEDRREFLAH
                     LGRENFQGSKTRDFASAWIKDPDTGETQPKLYRVGSKSLARCQYVALTHAQHAAVLVLD
                     IDVPSHQAGGKIEHVNPEVYAILERWARLEKAPAWIGVNPLSGKCQLIWLIDPVYAAAG
                     MSSPNMRLLAATTEEMTRVFGADQAFSHRLSRWPLHVSDDPTAYRWHAQHNRVDRLADL
                     MEVARMISGTEKPKKRYEQEFSSGRARIEAARKATAEAKALATLEASLPSAAEASGELI
                     DGVRVLWTAPGRAARDETAFRHALTVGYQLKAAGERLKDTKIIDAYERAYTVAQAVGAD
                     GREPDLPPMRDRQTMARRVRGYVAKGQPVVPARQTETQSSRGRKALATMGRRGGKKAAE
                     RWKDPNSEYARAQREKLAKSSQRQARKAKGNRLTIAGWFMTVEGETGSWRQSMKLCLNL
                     ACHVRP"
     gene            complement(463..1857)
                     /gene="rep"
                     /label=rep
     CDS             complement(2999..3529)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(3718..3746)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     4150..4166
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4215..5294)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(5295..5372)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        5390..5418
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    5426..5442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             5480..7468
                     /gene="A"
                     /label=A
                     /note="DDE-recombinase A from Escherichia phage Mu.
                     Accession#: P07636"
     CDS             7510..8445
                     /gene="B"
                     /label=B
                     /note="ATP-dependent target DNA activator B from
                     Escherichia phage Mu. Accession#: P03763"
     primer_bind     complement(8943..8959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8967..8983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8991..9021)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9036..9057)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9529..10117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc gccccatcat
       61 ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg gacggggatc
      121 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      181 agattatcaa aaaggatcat gcgagcaacc tccataagat cagctaggcg atctttggga
      241 gcagtccttg tcgcgttacg aggtgagccg gtggggaacc gttagctgcc tatggtgtga
      301 gcccccctag agagcttcaa gagcaatcag cccgacctag aaaggaggcc aagagagaga
      361 cctacggggg gaaccgtttt ctgcctacga gatgggcaca ttactgggaa gctttacggc
      421 gtcctcgtgg aagttcaatg cccgcagact taagtgctct attcacggtc tgacgtgaca
      481 cgctaaattc agacatagct tcattgattg tcgccacgag ccagtctctc cctcaacagt
      541 cataaaccaa cctgcaatgg tcaagcgatt tcctttagct ttcctagctt gtcgttgact
      601 ggacttagct agtttttctc gctgtgctcg ggcgtactca ctgtttgggt ctttccagcg
      661 ttctgcggcc tttttaccgc cacgtcttcc catagtggcc agagcttttc gccctcggct
      721 gctctgcgtc tctgtctgac gagcagggac gactggctgg cctttagcga cgtagccgcg
      781 cacacgtcgc gccatcgtct ggcggtcacg catcggcggc agatcaggct cacggccgtc
      841 tgctccgacc gcctgagcga cggtgtaggc acgctcgtag gcgtcgatga tcttggtgtc
      901 ttttaggcgc tcaccagccg cttttaactg gtatcccaca gtcaaagcgt ggcgaaaagc
      961 cgtctcatca cgggcggcac gccctggagc agtccagagg acacggacgc cgtcgatcag
     1021 ctctccagac gcttcagcgg cgctcggcag gcttgcttca agcgtggcaa gtgcttttgc
     1081 ttccgcagtg gcttttcttg ccgcttcgat acgtgcccgt ccgctagaaa actcctgctc
     1141 atagcgtttt ttaggttttt ctgtgcctga gatcatgcga gcaacctcca taagatcagc
     1201 taggcgatcc acgcgattgt gctgggcatg ccagcggtac gcggtgggat cgtcggagac
     1261 gtgcagtggc caccggctca gcctatgtga aaaagcctgg tcagcgccga aaacgcgggt
     1321 catttcctcg gtcgttgcag ccagcaggcg catattcggg ctgctcatgc ctgctgcggc
     1381 atacaccgga tcaatgagcc agatgagctg gcatttcccg ctcagtggat tcacgccgat
     1441 ccaagctggc gctttttcca ggcgtgccca gcgctccaaa atcgcgtaga cctcggggtt
     1501 tacgtgctcg attttcccgc cggcctggtg gctcggcaca tcaatgtcca ggacaagcac
     1561 ggctgcgtgc tgcgcgtgcg tcagagcaac atactggcac cgggcaagcg attttgaacc
     1621 aactcggtat aacttcggct gtgtttctcc cgtgtccggg tctttgatcc aagcgctggc
     1681 gaagtcgcgg gtcttgctgc cctggaaatt ttctctgccc aggtgagcga ggaattcgcg
     1741 gcggtcttcg ctcgtccagc cacgtgatcg cagcgcgagc tcgggatggg tgtcgaacag
     1801 atcagcggaa aatttccagg ccggtgtgtc aatgtctcgt gaatccgcta gagtcatttt
     1861 tgagcgcttt ctcccaggtt tggactgggg gttagccgac gccctgtgag ttaccgctca
     1921 cggggcgttc aacatttttc aggtattcgt gcagcttatc gcttcttgcc gcctgtgcgc
     1981 tttttcgacg cgcgacgctg ctgccgattc ggtgcaggtg gtggcggcgc tgacacgtcc
     2041 tgggcggcca cggccacacg aaacgcggca tttacgatgt ttgtcatgcc tgcgggcacc
     2101 gcgccacgat cgcggataat tctcgctgcc gcttccagct ctgtgacgac catggccaaa
     2161 atttcgctcg ggggacgcac ttccagcgcc atttgcgacc tagccgcctc cagctcctcg
     2221 gcgtggcgtt tgttggcgcg ctcgcggctg gctgcggcac gacacgcatc tgagcaatat
     2281 tttgcgcgcc gtcctcgcgg gtcaggccgg ggaggaatca ggccaccgca gtaggcgcaa
     2341 ctgattcgat cctccactac tgtgcgtcct cctggcgctg ccgagcacgc agctcgtcag
     2401 ccagctcctc aagatccgcc acgagagttt ctaggtcgct cgcggcactg gcccagtctc
     2461 gtgatgctgg cgcgtccgtc gtatcgagag ctcggaaaaa tccgatcacc gtttttaaat
     2521 cgacggcagc atcgagcgcg tcggactcca gcgcgacatc agagagatcc atagctgatg
     2581 attcgggcca attttggtac ttcgtcgtga aggtcatgac accattataa cgaacgttcg
     2641 ttaaagtttt tggcggaaaa tcacgcggca cgaaaatttt cacgaagcgg gactttgcgc
     2701 agctcagggg tgctaaaaat tttgtatcgc acttgatttt tccgaaagac agattatctg
     2761 caaacggtgt gtcgtatttc tggcttggtt tttaaaaaat ctggaatcga aaatttgcgg
     2821 ggcgacgcag aagtttttta cagctagcag cgggtcatgg gactcacgcc tgagcctagc
     2881 agcgggtgat gggactaacg cctgagttaa gccggagcgc tttgcggccg cggcgttgtg
     2941 acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg
     3001 tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat
     3061 agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa
     3121 gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt
     3181 gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacctgg
     3241 gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga
     3301 tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt
     3361 aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga
     3421 cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg
     3481 ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc
     3541 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     3601 gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     3661 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt
     3721 ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg
     3781 cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaaa
     3841 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgcacagag agctagataa
     3901 tagctgctga tgctcgatga gttttttcta atcagaattg gttaattggt tgtaacactg
     3961 gcagagcatt acgctgactt gacgggacgg cggctttgtt gaataaatcg cattcgccat
     4021 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     4081 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     4141 cacgacgttg taaaacgacg gccagtgaat tcgagctcgg taccagctaa ctcacattaa
     4201 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4261 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt
     4321 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc
     4381 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
     4441 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca
     4501 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
     4561 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
     4621 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg
     4681 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct
     4741 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg
     4801 tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac
     4861 gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag
     4921 ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct
     4981 tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga
     5041 gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg
     5101 ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc
     5161 agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg
     5221 ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac
     5281 gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata
     5341 ccgcgaaagg ttttgcacca ttcgatggtg tatattctga attgagctgt tgacaattaa
     5401 tcatcggctc gtataatgtg tggaattgtg agcggataac aatttcactc tagagatccc
     5461 catcaagcag gtgaataaca tggaactttg ggtatcaccg aaagagtgtg cgaatcttcc
     5521 tggtttgccg aaaacatcgg ctggtgtgat ttatgttgct aaaaagcaag gatggcaaaa
     5581 ccgcactaga gcaggtgtca aaggtggtaa agcaattgaa tacaatgcga actctttacc
     5641 tgttgaagcg aaagcggcgt tattgctgag acaaggagag attgaaacaa gcctggggta
     5701 ttttgaaatc gcccgcccca cgctggaagc ccatgattat gatcgtgagg cactgtggag
     5761 caaatgggat aacgccagcg attcccagcg cagacttgct gaaaaatggt tgcctgcggt
     5821 tcaggctgca gacgaaatgc tgaaccaggg gatttcaacg aaaacggctt ttgcgaccgt
     5881 tgcagggcat taccaggtca gcgcatccac tttgcgggac aagtattacc aggtacagaa
     5941 gtttgcgaag cctgactggg cggctgcact tgttgatgga cgtggagcat cccgtcgcaa
     6001 tgttcacaaa agtgaatttg acgaggatgc ctggcagttt ctgattgcag attatctgcg
     6061 accggaaaaa cccgctttcc gcaaatgtta tgagcgtctg gaactggcag cccgcgagca
     6121 tggctggagt attccctccc gtgccacggc ctttcgccgg attcagcaac tggacgaggc
     6181 aatggttgtt gcctgtcgtg aaggtgaaca tgcactgatg catctgatac cggcacagca
     6241 gcgaactgtg gaacacctgg acgccatgca gtggatcaac ggcgacggtt atctgcataa
     6301 cgtctttgta cgctggttta acggtgatgt gatccgtccg aaaacatggt tctggcagga
     6361 tgtgaaaacc cgaaaaattc tgggctggcg ctgcgatgtg agcgagaaca ttgattcaat
     6421 tcgcctctcg ttcatggatg ttgtgactcg ctacggtatc ccggaggatt ttcacatcac
     6481 cattgataac acccgtggtg ctgcgaataa atggctgacg ggaggcgcgc ccaatcgcta
     6541 ccgctttaag gtaaaagagg acgatccaaa aggactgttt ttactgatgg gggcgaaaat
     6601 gcactggaca agcgttgttg ccggtaaagg ctggggccag gcaaaacctg ttgaacgtgc
     6661 tttcggtgtt ggtgggcttg aggaatacgt tgataagcat ccggcactgg ctggcgcata
     6721 tacggggcca aatccgcagg caaaacctga taactatggc gaccgcgctg ttgatgcaga
     6781 gctgtttctg aaaacccttg ccgaaggtgt ggcgatgttc aatgccagaa caggccgtga
     6841 aacagaaatg tgcgggggca aactctcgtt tgatgatgtt ttcgagcgtg aatacgccag
     6901 aacgattgtg cgtaagccaa ccgaagaaca aaaacggatg ctgttactgc ctgccgaggc
     6961 ggtgaacgtt tcacgcaaag gcgagtttac gcttaaagtt ggcggctccc ttaaaggcgc
     7021 gaaaaacgtt tattacaaca tggcattaat gaatgccggc gtgaaaaaag ttgtggtcag
     7081 gtttgatccg cagcagctac acagcacggt ttattgctac accctggacg gtcggtttat
     7141 ctgtgaagcg gaatgtctgg cacctgttgc atttaatgat gctgcggcag gccgtgaata
     7201 tcgccgccgc cagaaacaac tgaaatctgc gacgaaagca gccattaagg cgcagaaaca
     7261 aatggacgcg ctggaagttg ctgaactgct gccgcagata gccgaaccag cagcaccaga
     7321 atcacgaatt gttggtattt tccggccttc cggtaatacg gaacgggtga agaatcagga
     7381 gcgtgatgat gaatacgaaa ctgagcgtga tgaatatctg aatcattcgc tggatattct
     7441 ggaacagaac agacgtaaaa aagccattta attaacgttt aaacaaaatt taattacgag
     7501 gttattcaga tgaatatttc cgatattcgc gcaggactgc gcacgcttgt agaaaatgaa
     7561 gaaaccacct ttaaacaaat tgctcttgag agcgggcttt ctaccggaac tatcagtagt
     7621 tttatcaatg ataagtacaa cggggataac gagcgtgttt cacaaatgct gcaacgctgg
     7681 ctggaaaaat atcatgcagt ggcagaacta cctgaaccgc cccgctttgt ggaaacgcag
     7741 acggtaaaac aaatctggac aagtatgcgt tttgccagcc tgactgaaag tattgctgtt
     7801 gtatgtggca atcctggtgt gggcaaaacc gaagcggccc gtgaatatcg ccgcaccaat
     7861 aacaatgtct ggatgatcac cattacgcca tcctgtgcca gcgttctgga atgtcttact
     7921 gaactggcgt ttgagctggg aatgaatgac gcaccacgcc gtaaagggcc gctctcccgc
     7981 gccctgcgac gtcgccttga aggtacacag gggctggtta tcatcgacga agctgatcat
     8041 cttggtgccg aagttctgga agaactccgc ctgttacagg aatcaacccg tattggcctt
     8101 gtgctgatgg gaaatcaccg ggtttattca aatatgacgg ggggtaacag aacggttgaa
     8161 tttgcccgtc tgttttcccg tattgcaaag cgcactgcaa ttaataaaac caaaaaagcc
     8221 gatgtaaaag ctattgcgga tgcctggcag attaacggcg aaaaagaact ggagttatta
     8281 cagcagattg cgcagaaacc aggtgcgctt cgcattctga atcattcact tcgccttgca
     8341 gccatgacgg ctcacggtaa aggtgagcgt gttaacgaag attatctgcg tcaggctttc
     8401 cgtgaattag acctggacgt tgatatttca acgctgctgc gtaattaaga aggagaagaa
     8461 attatgatgg cccgaaatat aaaaatggca acggatgcgc agaactggtt acaggctgac
     8521 tcttatacac aagtagcgtc ctgaacggaa cctttcccgt tttccaggat ctgatcttcc
     8581 atgtgacctc ctaacatggt aacgttcatg ataacttctg ctcttcatcg tgcggccgca
     8641 actagccggt tacgcatggg cagcatgatg agcaacgtga ttgcggtctg tgaccgcgaa
     8701 gccgatattc atgcttatct gcaggacaaa ctggcgcata acgagcaatt cgaggtgcct
     8761 gttgagcttc ttgtagttgc tgttctagct gtgccttggt tgccatgctt taagactcta
     8821 gtagctttcc tgcgatatgt catgcgcatg cgtagcaaac attgtcctgc aactcattca
     8881 ttatgtgcag tgctcctgtt actagagtcg acctgcaggc atgcaagctt ggcgtaatca
     8941 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9001 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9061 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9121 atcggccaac gcgcggggag aggcggtttg cgtattggcg aacttttgct gagttgaagg
     9181 atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa
     9241 tcagtaaccg tcagtgccga taagttcaaa gttaaacctg gtgttgatac caacattgaa
     9301 acgctgatcg aaaacgcgct gaaaaacgct gctgaatgtg cgagcttctt ccgcttcctc
     9361 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9421 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9481 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9541 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9601 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9661 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9721 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9781 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9841 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9901 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9961 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10021 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10081 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10141 ggggtctgac gctcagtgga acgatccgtc ga
//
