LOCUS       40924_45968        9333 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Suicide vector pVIK112, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9333)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9333)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..66
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             68..3139
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             3194..4444
                     /gene="lacY"
                     /label=lacY
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     CDS             4511..5058
                     /codon_start=1
                     /note="unnamed protein product; lacA fragment"
                     /protein_id="SJL88074.1"
                     /translation="LNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEVE
                     KRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNVLI
                     APNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGAGSI
                     VTKDIPPNVVAAGVPCRVI"
     CDS             5414..6205
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(7765..8133)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(8166..8275)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      8919..9275
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gaattcccgg gagagctcga tatcgcatgc ggtacctcta gatgactgac aggaaacagc
       61 tatgaccatg gtcgacctgc agccaagctt cnnngtcgtt ttacaacgtc gtgactggga
      121 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      181 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      241 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
      301 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
      361 gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga
      421 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
      481 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
      541 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt
      601 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct
      661 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa
      721 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg
      781 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac
      841 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
      901 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa
      961 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
     1021 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
     1081 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca
     1141 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct
     1201 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct
     1261 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac
     1321 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag
     1381 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc
     1441 gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc
     1501 tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga
     1561 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa
     1621 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga
     1681 atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg
     1741 tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa
     1801 atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa
     1861 cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct
     1921 gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga
     1981 agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc
     2041 gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa
     2101 acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac
     2161 agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg
     2221 gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat
     2281 cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca
     2341 atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct
     2401 gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga
     2461 agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca
     2521 ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac
     2581 gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg
     2641 gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc
     2701 gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg
     2761 gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg
     2821 ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct
     2881 gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt
     2941 caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca
     3001 cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga
     3061 ctcctggagc ccgtcagtat cggcgnnnnn ncagctgagc gccggtcgct accattacca
     3121 gttggtctgg tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt
     3181 aaggaaatcc attatgtact atttaaaaaa cacaaacttt tggatgttcg gtttattctt
     3241 tttcttttac ttttttatca tgggagccta cttcccgttt ttcccgattt ggctacatga
     3301 catcaaccat atcagcaaaa gtgatacggg tattattttt gccgctattt ctctgttctc
     3361 gctattattc caaccgctgt ttggtctgct ttctgacaaa ctcgggctgc gcaaatacct
     3421 gctgtggatt attaccggca tgttagtgat gtttgcgccg ttctttattt ttatcttcgg
     3481 gccactgtta caatacaaca ttttagtagg atcgattgtt ggtggtattt atctaggctt
     3541 ttgttttaac gccggtgcgc cagcagtaga ggcatttatt gagaaagtca gccgtcgcag
     3601 taatttcgaa tttggtcgcg cgcggatgtt tggctgtgtt ggctgggcgc tgtgtgcctc
     3661 gattgtcggc atcatgttca ccatcaataa tcagtttgtt ttctggctgg gctctggctg
     3721 tgcactcatc ctcgccgttt tactcttttt cgccaaaacg gatgcgccct cttctgccac
     3781 ggttgccaat gcggtaggtg ccaaccattc ggcatttagc cttaagctgg cactggaact
     3841 gttcagacag ccaaaactgt ggtttttgtc actgtatgtt attggcgttt cctgcaccta
     3901 cgatgttttt gaccaacagt ttgctaattt ctttacttcg ttctttgcta ccggtgaaca
     3961 gggtacgcgg gtatttggct acgtaacgac aatgggcgaa ttacttaacg cctcgattat
     4021 gttctttgcg ccactgatca ttaatcgcat cggtgggaaa aacgccctgc tgctggctgg
     4081 cactattatg tctgtacgta ttattggctc atcgttcgcc acctcagcgc tggaagtggt
     4141 tattctgaaa acgctgcata tgtttgaagt accgttcctg ctggtgggct gctttaaata
     4201 tattaccagc cagtttgaag tgcgtttttc agcgacgatt tatctggtct gtttctgctt
     4261 ctttaagcaa ctggcgatga tttttatgtc tgtactggcg ggcaatatgt atgaaagcat
     4321 cggtttccag ggcgcttatc tggtgctggg tctggtggcg ctgggcttca ccttaatttc
     4381 cgtgttcacg cttagcggcc ccggcccgct ttccctgctg cgtcgtcagg tgaatgaagt
     4441 cgcttaagca atcaatgtcg gatgcggcgc gacgcttatc cgaccaacat atcataacgg
     4501 agtgatcgca ttgaacatgc caatgaccga aagaataaga gcaggcaagc tatttaccga
     4561 tatgtgcgaa ggcttaccgg aaaaaagact tcgtgggaaa acgttaatgt atgagtttaa
     4621 tcactcgcat ccatcagaag ttgaaaaaag agaaagcctg attaaagaaa tgtttgccac
     4681 ggtaggggaa aacgcctggg tagaaccgcc tgtctatttc tcttacggtt ccaacatcca
     4741 tataggccgc aatttttatg caaatttcaa tttaaccatt gtcgatgact acacggtaac
     4801 aatcggtgat aacgtactga ttgcacccaa cgttactctt tccgttacgg gacaccctgt
     4861 acaccatgaa ttgagaaaaa acggcgagat gtactctttt ccgataacga ttggcaataa
     4921 cgtctggatc ggaagtcatg tggttattaa tccaggcgtc accatcgggg ataattctgt
     4981 tattggcgcg ggtagtatcg tcacaaaaga cattccacca aacgtcgtgg cggctggcgt
     5041 tccttgtcgg gttattcgag cttcacgctg ccgcaagcac tcagggcgca agggctgcta
     5101 aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
     5161 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
     5221 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
     5281 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
     5341 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg
     5401 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5461 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5521 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5581 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5641 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5701 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     5761 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     5821 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     5881 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     5941 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6001 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6061 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6121 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6181 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     6241 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6301 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6361 ctggagttct tcgcccaccc cgcaatggca acaacgttgc gcaaactatt aactggcgaa
     6421 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6481 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6541 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6601 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6661 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6721 atactttaga ttgatttaaa acttcatttt taattttggt gcactctcag tacaatctgc
     6781 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     6841 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     6901 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     6961 cgtcatcacc gaaacgcgcg aggcagcaag gagatggcgc ccaacagtcc cccggccacg
     7021 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     7081 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     7141 atgccggcca cgatgcgtcc ggcgtagagg atccagccga ccaggctttc cacgcccgcg
     7201 tgccgctcca tgtcgttcgc gcggttctcg gaaacgcgct gccgcgtttc gtgattgtca
     7261 cgctcaagcc cgtagtcccg ttcgagcgtc gcgcagaggt cagcgagggc gcggtaggcc
     7321 cgatacggct catggatggt gtttcgggtc gggtgaatct tgttgatggc gatatggatg
     7381 tgcaggttgt cggtgtcgtg atgcacggca ctgacgcgct gatgctcggc gaagccaagc
     7441 ccagcgcaga tgcggtcctc aatcgcgcgc aacgtctccg cgtcgggctt ctctcccgcg
     7501 cggaagctaa ccagcaggtg ataggtcttg tcggcctcgg aacgggtgtt gccgtgctgg
     7561 gtcgccatca cctcggccat gacagcgggc agggtgtttg cctcgcagtt cgtgacgcgc
     7621 acgtgaccca ggcgctcggt cttgccttgc tcgtcggtga tgtacttcac cagctccgcg
     7681 aagtcgctct tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg caccccccgg
     7741 ccgttttagc ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc ccatcatgac
     7801 cttgccaagc tcgtcctgct tctcttcgat cttcgccagc agggcgagga tcgtggcatc
     7861 accgaaccgc gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg
     7921 gcccaggtcg ccattgatgc gggccagctc gcggacgtgc tcatagtcca cgacgcccgt
     7981 gattttgtag ccctggccga cggccagcag gtaggccgac aggctcatgc cggccgccgc
     8041 cgccttttcc tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga taggtgggct
     8101 gcccttcctg gttggcttgg tttcatcagc catccgcttg ccctcatctg ttacgccggc
     8161 ggtagccggc cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg
     8221 gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg
     8281 acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg
     8341 tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc
     8401 agcggaaaag cgctgcttcc ctgctgtttt gtggaatatc taccgactgg aaacaggcaa
     8461 atgcaggaaa ttactgaact gaggggacag gcgagagacg atgccaaaga gctacaccga
     8521 cgagctggcc gagtgggttg aatcccgcgc ggccaagaag cgccggcgtg atgaggctgc
     8581 ggttgcgttc ctggcggtga gggcggatgt cgaggcggcg ttagcgtccg gctatgcgct
     8641 cgtcaccatt tgggagcaca tgcgggaaac ggggaaggtc aagttctcct acgagacgtt
     8701 ccgctcgcac gccaggcggc acatcaaggc caagcccgcc gatgtgcccg caccgcaggc
     8761 caaggctgcg gaacccgcgc cggcacccaa gacgccggag ccacggcggc cgaagcaggg
     8821 gggcaaggct gaaaagccgg cccccgctgc ggccccgacc ggcttcacct tcaacccaac
     8881 accggacaaa aaggatccag cagttcaacc tgttgatagt acgtactaag ctctcatgtt
     8941 tcacgtacta agctctcatg tttaacgtac taagctctca tgtttaacga actaaaccct
     9001 catggctaac gtactaagct ctcatggcta acgtactaag ctctcatgtt tcacgtacta
     9061 agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag cctctaaggt
     9121 tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagctttta aggtttaacg
     9181 gttgtggaca acaagccagg gatgtaacgc actgagaagc ccttagagcc tctcaaagca
     9241 attttcagtg acacaggaac acttaacggc tgacatggcc ttagaaggcg ttttagaagc
     9301 cctaatggat ggagtcaaaa caaactagcg atc
//
