LOCUS       V002241                 9336 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002241
VERSION     V002241
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9336)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9336)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9336)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9336)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             98..3142
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             3197..4447
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     CDS             4514..5061
                     /codon_start=1
                     /note="unnamed protein product; lacA fragment"
                     /protein_id="SJL88070.1"
                     /translation="LNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEVE
                     KRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNVLI
                     APNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGAGSI
                     VTKDIPPNVVAAGVPCRVI"
     CDS             5417..6208
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(7768..8136)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(8169..8278)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      8922..9278
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gaattcccgg gagagctcga tatcgcatgc ggtacctcta gaagaagctt gggatccgtc
       61 gaccggggat ccgtcgacct gcagccaagc ttgcnnngtc gttttacaac gtcgtgactg
      121 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      181 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      241 cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg
      301 cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga
      361 tgcgcccatc tacaccaacg taacctatcc cattacggtc aatccgccgt ttgttcccac
      421 ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga
      481 aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg
      541 gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt
      601 tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgttggagtg acggcagtta
      661 tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca
      721 taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag
      781 ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt
      841 aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg
      901 tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga
      961 aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca
     1021 caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg
     1081 gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg
     1141 tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat
     1201 cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc
     1261 gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga
     1321 aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat
     1381 gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg
     1441 gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa
     1501 atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac
     1561 cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc
     1621 gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg
     1681 cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt
     1741 tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat
     1801 taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc
     1861 gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc
     1921 gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat
     1981 cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt
     2041 ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg
     2101 taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct
     2161 cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc
     2221 ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc
     2281 catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg
     2341 gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact
     2401 gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag
     2461 tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta
     2521 ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat
     2581 tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta
     2641 ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac
     2701 accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa
     2761 ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga
     2821 ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg
     2881 tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca
     2941 gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct
     3001 gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga
     3061 cgactcctgg agcccgtcag tatcggcgnn nnnncagctg agcgccggtc gctaccatta
     3121 ccagttggtc tggtgtcaaa aataataata accgggcagg ccatgtctgc ccgtatttcg
     3181 cgtaaggaaa tccattatgt actatttaaa aaacacaaac ttttggatgt tcggtttatt
     3241 ctttttcttt tactttttta tcatgggagc ctacttcccg tttttcccga tttggctaca
     3301 tgacatcaac catatcagca aaagtgatac gggtattatt tttgccgcta tttctctgtt
     3361 ctcgctatta ttccaaccgc tgtttggtct gctttctgac aaactcgggc tgcgcaaata
     3421 cctgctgtgg attattaccg gcatgttagt gatgtttgcg ccgttcttta tttttatctt
     3481 cgggccactg ttacaataca acattttagt aggatcgatt gttggtggta tttatctagg
     3541 cttttgtttt aacgccggtg cgccagcagt agaggcattt attgagaaag tcagccgtcg
     3601 cagtaatttc gaatttggtc gcgcgcggat gtttggctgt gttggctggg cgctgtgtgc
     3661 ctcgattgtc ggcatcatgt tcaccatcaa taatcagttt gttttctggc tgggctctgg
     3721 ctgtgcactc atcctcgccg ttttactctt tttcgccaaa acggatgcgc cctcttctgc
     3781 cacggttgcc aatgcggtag gtgccaacca ttcggcattt agccttaagc tggcactgga
     3841 actgttcaga cagccaaaac tgtggttttt gtcactgtat gttattggcg tttcctgcac
     3901 ctacgatgtt tttgaccaac agtttgctaa tttctttact tcgttctttg ctaccggtga
     3961 acagggtacg cgggtatttg gctacgtaac gacaatgggc gaattactta acgcctcgat
     4021 tatgttcttt gcgccactga tcattaatcg catcggtggg aaaaacgccc tgctgctggc
     4081 tggcactatt atgtctgtac gtattattgg ctcatcgttc gccacctcag cgctggaagt
     4141 ggttattctg aaaacgctgc atatgtttga agtaccgttc ctgctggtgg gctgctttaa
     4201 atatattacc agccagtttg aagtgcgttt ttcagcgacg atttatctgg tctgtttctg
     4261 cttctttaag caactggcga tgatttttat gtctgtactg gcgggcaata tgtatgaaag
     4321 catcggtttc cagggcgctt atctggtgct gggtctggtg gcgctgggct tcaccttaat
     4381 ttccgtgttc acgcttagcg gccccggccc gctttccctg ctgcgtcgtc aggtgaatga
     4441 agtcgcttaa gcaatcaatg tcggatgcgg cgcgacgctt atccgaccaa catatcataa
     4501 cggagtgatc gcattgaaca tgccaatgac cgaaagaata agagcaggca agctatttac
     4561 cgatatgtgc gaaggcttac cggaaaaaag acttcgtggg aaaacgttaa tgtatgagtt
     4621 taatcactcg catccatcag aagttgaaaa aagagaaagc ctgattaaag aaatgtttgc
     4681 cacggtaggg gaaaacgcct gggtagaacc gcctgtctat ttctcttacg gttccaacat
     4741 ccatataggc cgcaattttt atgcaaattt caatttaacc attgtcgatg actacacggt
     4801 aacaatcggt gataacgtac tgattgcacc caacgttact ctttccgtta cgggacaccc
     4861 tgtacaccat gaattgagaa aaaacggcga gatgtactct tttccgataa cgattggcaa
     4921 taacgtctgg atcggaagtc atgtggttat taatccaggc gtcaccatcg gggataattc
     4981 tgttattggc gcgggtagta tcgtcacaaa agacattcca ccaaacgtcg tggcggctgg
     5041 cgttccttgt cgggttattc gagcttcacg ctgccgcaag cactcagggc gcaagggctg
     5101 ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa
     5161 tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc
     5221 ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc
     5281 ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat
     5341 ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg
     5401 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     5461 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5521 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5581 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5641 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5701 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5761 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5821 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5881 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5941 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     6001 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     6061 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     6121 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     6181 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     6241 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6301 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6361 atgctggagt tcttcgccca ccccgcaatg gcaacaacgt tgcgcaaact attaactggc
     6421 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6481 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6541 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6601 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6661 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6721 tatatacttt agattgattt aaaacttcat ttttaatttt ggtgcactct cagtacaatc
     6781 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     6841 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     6901 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     6961 caccgtcatc accgaaacgc gcgaggcagc aaggagatgg cgcccaacag tcccccggcc
     7021 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     7081 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     7141 gtgatgccgg ccacgatgcg tccggcgtag aggatccagc cgaccaggct ttccacgccc
     7201 gcgtgccgct ccatgtcgtt cgcgcggttc tcggaaacgc gctgccgcgt ttcgtgattg
     7261 tcacgctcaa gcccgtagtc ccgttcgagc gtcgcgcaga ggtcagcgag ggcgcggtag
     7321 gcccgatacg gctcatggat ggtgtttcgg gtcgggtgaa tcttgttgat ggcgatatgg
     7381 atgtgcaggt tgtcggtgtc gtgatgcacg gcactgacgc gctgatgctc ggcgaagcca
     7441 agcccagcgc agatgcggtc ctcaatcgcg cgcaacgtct ccgcgtcggg cttctctccc
     7501 gcgcggaagc taaccagcag gtgataggtc ttgtcggcct cggaacgggt gttgccgtgc
     7561 tgggtcgcca tcacctcggc catgacagcg ggcagggtgt ttgcctcgca gttcgtgacg
     7621 cgcacgtgac ccaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
     7681 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
     7741 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
     7801 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
     7861 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
     7921 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
     7981 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
     8041 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
     8101 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
     8161 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
     8221 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
     8281 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
     8341 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
     8401 tgcagcggaa aagcgctgct tccctgctgt tttgtggaat atctaccgac tggaaacagg
     8461 caaatgcagg aaattactga actgagggga caggcgagag acgatgccaa agagctacac
     8521 cgacgagctg gccgagtggg ttgaatcccg cgcggccaag aagcgccggc gtgatgaggc
     8581 tgcggttgcg ttcctggcgg tgagggcgga tgtcgaggcg gcgttagcgt ccggctatgc
     8641 gctcgtcacc atttgggagc acatgcggga aacggggaag gtcaagttct cctacgagac
     8701 gttccgctcg cacgccaggc ggcacatcaa ggccaagccc gccgatgtgc ccgcaccgca
     8761 ggccaaggct gcggaacccg cgccggcacc caagacgccg gagccacggc ggccgaagca
     8821 ggggggcaag gctgaaaagc cggcccccgc tgcggccccg accggcttca ccttcaaccc
     8881 aacaccggac aaaaaggatc cagcagttca acctgttgat agtacgtact aagctctcat
     8941 gtttcacgta ctaagctctc atgtttaacg tactaagctc tcatgtttaa cgaactaaac
     9001 cctcatggct aacgtactaa gctctcatgg ctaacgtact aagctctcat gtttcacgta
     9061 ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa tagcctctaa
     9121 ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagctt ttaaggttta
     9181 acggttgtgg acaacaagcc agggatgtaa cgcactgaga agcccttaga gcctctcaaa
     9241 gcaattttca gtgacacagg aacacttaac ggctgacatg gccttagaag gcgttttaga
     9301 agccctaatg gatggagtca aaacaaacta gcgatc
//
