LOCUS       V002242                 9334 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002242
VERSION     V002242
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9334)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9334)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             90..3140
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             3195..4445
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     CDS             4512..5059
                     /codon_start=1
                     /note="unnamed protein product; lacA fragment"
                     /protein_id="SJL88066.1"
                     /translation="LNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEVE
                     KRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNVLI
                     APNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGAGSI
                     VTKDIPPNVVAAGVPCRVI"
     CDS             5415..6206
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(7766..8134)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(8167..8276)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      8920..9276
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gaattcccgg gagagctcga tatcgcatgc ggtacctcta gaagaagctt gggatccgtc
       61 gaccggggat ccgtcgacct gcagccaagc ttnnngtcgt tttacaacgt cgtgactggg
      121 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
      181 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      241 aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg
      301 atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg
      361 cgcccatcta caccaacgta acctatccca ttacggtcaa tccgccgttt gttcccacgg
      421 agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag
      481 gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc
      541 gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt
      601 tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ttggagtgac ggcagttatc
      661 tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata
      721 aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc
      781 gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa
      841 cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg
      901 aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa
      961 acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca
     1021 ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga
     1081 ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc
     1141 acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc
     1201 tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc
     1261 tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa
     1321 cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga
     1381 gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt
     1441 cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat
     1501 ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg
     1561 atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga
     1621 aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg
     1681 aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc
     1741 gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta
     1801 aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga
     1861 acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc
     1921 tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg
     1981 aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg
     2041 cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta
     2101 aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca
     2161 cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct
     2221 ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca
     2281 tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc
     2341 aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc
     2401 tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg
     2461 aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc
     2521 aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta
     2581 cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc
     2641 ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac
     2701 cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact
     2761 ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc
     2821 gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc
     2881 tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt
     2941 tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc
     3001 acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg
     3061 actcctggag cccgtcagta tcggcgnnnn nncagctgag cgccggtcgc taccattacc
     3121 agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc gtatttcgcg
     3181 taaggaaatc cattatgtac tatttaaaaa acacaaactt ttggatgttc ggtttattct
     3241 ttttctttta cttttttatc atgggagcct acttcccgtt tttcccgatt tggctacatg
     3301 acatcaacca tatcagcaaa agtgatacgg gtattatttt tgccgctatt tctctgttct
     3361 cgctattatt ccaaccgctg tttggtctgc tttctgacaa actcgggctg cgcaaatacc
     3421 tgctgtggat tattaccggc atgttagtga tgtttgcgcc gttctttatt tttatcttcg
     3481 ggccactgtt acaatacaac attttagtag gatcgattgt tggtggtatt tatctaggct
     3541 tttgttttaa cgccggtgcg ccagcagtag aggcatttat tgagaaagtc agccgtcgca
     3601 gtaatttcga atttggtcgc gcgcggatgt ttggctgtgt tggctgggcg ctgtgtgcct
     3661 cgattgtcgg catcatgttc accatcaata atcagtttgt tttctggctg ggctctggct
     3721 gtgcactcat cctcgccgtt ttactctttt tcgccaaaac ggatgcgccc tcttctgcca
     3781 cggttgccaa tgcggtaggt gccaaccatt cggcatttag ccttaagctg gcactggaac
     3841 tgttcagaca gccaaaactg tggtttttgt cactgtatgt tattggcgtt tcctgcacct
     3901 acgatgtttt tgaccaacag tttgctaatt tctttacttc gttctttgct accggtgaac
     3961 agggtacgcg ggtatttggc tacgtaacga caatgggcga attacttaac gcctcgatta
     4021 tgttctttgc gccactgatc attaatcgca tcggtgggaa aaacgccctg ctgctggctg
     4081 gcactattat gtctgtacgt attattggct catcgttcgc cacctcagcg ctggaagtgg
     4141 ttattctgaa aacgctgcat atgtttgaag taccgttcct gctggtgggc tgctttaaat
     4201 atattaccag ccagtttgaa gtgcgttttt cagcgacgat ttatctggtc tgtttctgct
     4261 tctttaagca actggcgatg atttttatgt ctgtactggc gggcaatatg tatgaaagca
     4321 tcggtttcca gggcgcttat ctggtgctgg gtctggtggc gctgggcttc accttaattt
     4381 ccgtgttcac gcttagcggc cccggcccgc tttccctgct gcgtcgtcag gtgaatgaag
     4441 tcgcttaagc aatcaatgtc ggatgcggcg cgacgcttat ccgaccaaca tatcataacg
     4501 gagtgatcgc attgaacatg ccaatgaccg aaagaataag agcaggcaag ctatttaccg
     4561 atatgtgcga aggcttaccg gaaaaaagac ttcgtgggaa aacgttaatg tatgagttta
     4621 atcactcgca tccatcagaa gttgaaaaaa gagaaagcct gattaaagaa atgtttgcca
     4681 cggtagggga aaacgcctgg gtagaaccgc ctgtctattt ctcttacggt tccaacatcc
     4741 atataggccg caatttttat gcaaatttca atttaaccat tgtcgatgac tacacggtaa
     4801 caatcggtga taacgtactg attgcaccca acgttactct ttccgttacg ggacaccctg
     4861 tacaccatga attgagaaaa aacggcgaga tgtactcttt tccgataacg attggcaata
     4921 acgtctggat cggaagtcat gtggttatta atccaggcgt caccatcggg gataattctg
     4981 ttattggcgc gggtagtatc gtcacaaaag acattccacc aaacgtcgtg gcggctggcg
     5041 ttccttgtcg ggttattcga gcttcacgct gccgcaagca ctcagggcgc aagggctgct
     5101 aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
     5161 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
     5221 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
     5281 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     5341 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat
     5401 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     5461 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     5521 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     5581 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     5641 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     5701 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     5761 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     5821 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     5881 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5941 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     6001 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     6061 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     6121 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     6181 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     6241 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     6301 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     6361 gctggagttc ttcgcccacc ccgcaatggc aacaacgttg cgcaaactat taactggcga
     6421 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6481 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6541 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6601 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6661 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6721 tatactttag attgatttaa aacttcattt ttaattttgg tgcactctca gtacaatctg
     6781 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     6841 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     6901 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     6961 ccgtcatcac cgaaacgcgc gaggcagcaa ggagatggcg cccaacagtc ccccggccac
     7021 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
     7081 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
     7141 gatgccggcc acgatgcgtc cggcgtagag gatccagccg accaggcttt ccacgcccgc
     7201 gtgccgctcc atgtcgttcg cgcggttctc ggaaacgcgc tgccgcgttt cgtgattgtc
     7261 acgctcaagc ccgtagtccc gttcgagcgt cgcgcagagg tcagcgaggg cgcggtaggc
     7321 ccgatacggc tcatggatgg tgtttcgggt cgggtgaatc ttgttgatgg cgatatggat
     7381 gtgcaggttg tcggtgtcgt gatgcacggc actgacgcgc tgatgctcgg cgaagccaag
     7441 cccagcgcag atgcggtcct caatcgcgcg caacgtctcc gcgtcgggct tctctcccgc
     7501 gcggaagcta accagcaggt gataggtctt gtcggcctcg gaacgggtgt tgccgtgctg
     7561 ggtcgccatc acctcggcca tgacagcggg cagggtgttt gcctcgcagt tcgtgacgcg
     7621 cacgtgaccc aggcgctcgg tcttgccttg ctcgtcggtg atgtacttca ccagctccgc
     7681 gaagtcgctc ttcttgatgg agcgcatggg gacgtgcttg gcaatcacgc gcaccccccg
     7741 gccgttttag cggctaaaaa agtcatggct ctgccctcgg gcggaccacg cccatcatga
     7801 ccttgccaag ctcgtcctgc ttctcttcga tcttcgccag cagggcgagg atcgtggcat
     7861 caccgaaccg cgccgtgcgc gggtcgtcgg tgagccagag tttcagcagg ccgcccaggc
     7921 ggcccaggtc gccattgatg cgggccagct cgcggacgtg ctcatagtcc acgacgcccg
     7981 tgattttgta gccctggccg acggccagca ggtaggccga caggctcatg ccggccgccg
     8041 ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca gtacaccttg ataggtgggc
     8101 tgcccttcct ggttggcttg gtttcatcag ccatccgctt gccctcatct gttacgccgg
     8161 cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag
     8221 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct
     8281 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc
     8341 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg
     8401 cagcggaaaa gcgctgcttc cctgctgttt tgtggaatat ctaccgactg gaaacaggca
     8461 aatgcaggaa attactgaac tgaggggaca ggcgagagac gatgccaaag agctacaccg
     8521 acgagctggc cgagtgggtt gaatcccgcg cggccaagaa gcgccggcgt gatgaggctg
     8581 cggttgcgtt cctggcggtg agggcggatg tcgaggcggc gttagcgtcc ggctatgcgc
     8641 tcgtcaccat ttgggagcac atgcgggaaa cggggaaggt caagttctcc tacgagacgt
     8701 tccgctcgca cgccaggcgg cacatcaagg ccaagcccgc cgatgtgccc gcaccgcagg
     8761 ccaaggctgc ggaacccgcg ccggcaccca agacgccgga gccacggcgg ccgaagcagg
     8821 ggggcaaggc tgaaaagccg gcccccgctg cggccccgac cggcttcacc ttcaacccaa
     8881 caccggacaa aaaggatcca gcagttcaac ctgttgatag tacgtactaa gctctcatgt
     8941 ttcacgtact aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc
     9001 tcatggctaa cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact
     9061 aagctctcat gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg
     9121 ttttaagttt tataagaaaa aaaagaatat ataaggcttt taaagctttt aaggtttaac
     9181 ggttgtggac aacaagccag ggatgtaacg cactgagaag cccttagagc ctctcaaagc
     9241 aattttcagt gacacaggaa cacttaacgg ctgacatggc cttagaaggc gttttagaag
     9301 ccctaatgga tggagtcaaa acaaactagc gatc
//
