LOCUS       40924_45953        9335 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Suicide vector pVIK107, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9335)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9335)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9335)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             88..3141
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             3196..4446
                     /gene="lacY"
                     /label=lacY
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     CDS             4513..5060
                     /codon_start=1
                     /note="unnamed protein product; lacA fragment"
                     /protein_id="SJL88062.1"
                     /translation="LNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEVE
                     KRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNVLI
                     APNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGAGSI
                     VTKDIPPNVVAAGVPCRVI"
     CDS             5416..6207
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(7767..8135)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(8168..8277)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      8921..9277
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gaattcccgg gagagctcga tatcgcatgc ggtacctcta gaagaagctt gggatccgtc
       61 gaccggggat ccgtcgacct gcagccaagc ttcnnngtcg ttttacaacg tcgtgactgg
      121 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      181 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      241 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc
      301 gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat
      361 gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt tgttcccacg
      421 gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa
      481 ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg
      541 cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt
      601 ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gttggagtga cggcagttat
      661 ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat
      721 aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc
      781 cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta
      841 acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt
      901 gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa
      961 aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac
     1021 accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg
     1081 attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt
     1141 cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc
     1201 ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg
     1261 ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa
     1321 acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg
     1381 agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg
     1441 tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa
     1501 tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc
     1561 gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg
     1621 aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc
     1681 gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt
     1741 cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt
     1801 aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg
     1861 aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg
     1921 ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc
     1981 gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg
     2041 gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt
     2101 aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc
     2161 acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc
     2221 tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc
     2281 atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg
     2341 caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg
     2401 ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt
     2461 gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac
     2521 caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt
     2581 acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac
     2641 cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca
     2701 ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac
     2761 tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac
     2821 cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt
     2881 ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag
     2941 ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg
     3001 cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac
     3061 gactcctgga gcccgtcagt atcggcgnnn nnncagctga gcgccggtcg ctaccattac
     3121 cagttggtct ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc cgtatttcgc
     3181 gtaaggaaat ccattatgta ctatttaaaa aacacaaact tttggatgtt cggtttattc
     3241 tttttctttt acttttttat catgggagcc tacttcccgt ttttcccgat ttggctacat
     3301 gacatcaacc atatcagcaa aagtgatacg ggtattattt ttgccgctat ttctctgttc
     3361 tcgctattat tccaaccgct gtttggtctg ctttctgaca aactcgggct gcgcaaatac
     3421 ctgctgtgga ttattaccgg catgttagtg atgtttgcgc cgttctttat ttttatcttc
     3481 gggccactgt tacaatacaa cattttagta ggatcgattg ttggtggtat ttatctaggc
     3541 ttttgtttta acgccggtgc gccagcagta gaggcattta ttgagaaagt cagccgtcgc
     3601 agtaatttcg aatttggtcg cgcgcggatg tttggctgtg ttggctgggc gctgtgtgcc
     3661 tcgattgtcg gcatcatgtt caccatcaat aatcagtttg ttttctggct gggctctggc
     3721 tgtgcactca tcctcgccgt tttactcttt ttcgccaaaa cggatgcgcc ctcttctgcc
     3781 acggttgcca atgcggtagg tgccaaccat tcggcattta gccttaagct ggcactggaa
     3841 ctgttcagac agccaaaact gtggtttttg tcactgtatg ttattggcgt ttcctgcacc
     3901 tacgatgttt ttgaccaaca gtttgctaat ttctttactt cgttctttgc taccggtgaa
     3961 cagggtacgc gggtatttgg ctacgtaacg acaatgggcg aattacttaa cgcctcgatt
     4021 atgttctttg cgccactgat cattaatcgc atcggtggga aaaacgccct gctgctggct
     4081 ggcactatta tgtctgtacg tattattggc tcatcgttcg ccacctcagc gctggaagtg
     4141 gttattctga aaacgctgca tatgtttgaa gtaccgttcc tgctggtggg ctgctttaaa
     4201 tatattacca gccagtttga agtgcgtttt tcagcgacga tttatctggt ctgtttctgc
     4261 ttctttaagc aactggcgat gatttttatg tctgtactgg cgggcaatat gtatgaaagc
     4321 atcggtttcc agggcgctta tctggtgctg ggtctggtgg cgctgggctt caccttaatt
     4381 tccgtgttca cgcttagcgg ccccggcccg ctttccctgc tgcgtcgtca ggtgaatgaa
     4441 gtcgcttaag caatcaatgt cggatgcggc gcgacgctta tccgaccaac atatcataac
     4501 ggagtgatcg cattgaacat gccaatgacc gaaagaataa gagcaggcaa gctatttacc
     4561 gatatgtgcg aaggcttacc ggaaaaaaga cttcgtggga aaacgttaat gtatgagttt
     4621 aatcactcgc atccatcaga agttgaaaaa agagaaagcc tgattaaaga aatgtttgcc
     4681 acggtagggg aaaacgcctg ggtagaaccg cctgtctatt tctcttacgg ttccaacatc
     4741 catataggcc gcaattttta tgcaaatttc aatttaacca ttgtcgatga ctacacggta
     4801 acaatcggtg ataacgtact gattgcaccc aacgttactc tttccgttac gggacaccct
     4861 gtacaccatg aattgagaaa aaacggcgag atgtactctt ttccgataac gattggcaat
     4921 aacgtctgga tcggaagtca tgtggttatt aatccaggcg tcaccatcgg ggataattct
     4981 gttattggcg cgggtagtat cgtcacaaaa gacattccac caaacgtcgt ggcggctggc
     5041 gttccttgtc gggttattcg agcttcacgc tgccgcaagc actcagggcg caagggctgc
     5101 taaaggaagc ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     5161 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct
     5221 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg
     5281 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
     5341 gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga
     5401 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5461 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5521 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5581 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5641 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5701 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5761 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5821 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5881 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5941 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     6001 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     6061 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     6121 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     6181 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     6241 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     6301 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     6361 tgctggagtt cttcgcccac cccgcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6421 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6481 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6541 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6601 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6661 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6721 atatacttta gattgattta aaacttcatt tttaattttg gtgcactctc agtacaatct
     6781 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     6841 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     6901 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     6961 accgtcatca ccgaaacgcg cgaggcagca aggagatggc gcccaacagt cccccggcca
     7021 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
     7081 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
     7141 tgatgccggc cacgatgcgt ccggcgtaga ggatccagcc gaccaggctt tccacgcccg
     7201 cgtgccgctc catgtcgttc gcgcggttct cggaaacgcg ctgccgcgtt tcgtgattgt
     7261 cacgctcaag cccgtagtcc cgttcgagcg tcgcgcagag gtcagcgagg gcgcggtagg
     7321 cccgatacgg ctcatggatg gtgtttcggg tcgggtgaat cttgttgatg gcgatatgga
     7381 tgtgcaggtt gtcggtgtcg tgatgcacgg cactgacgcg ctgatgctcg gcgaagccaa
     7441 gcccagcgca gatgcggtcc tcaatcgcgc gcaacgtctc cgcgtcgggc ttctctcccg
     7501 cgcggaagct aaccagcagg tgataggtct tgtcggcctc ggaacgggtg ttgccgtgct
     7561 gggtcgccat cacctcggcc atgacagcgg gcagggtgtt tgcctcgcag ttcgtgacgc
     7621 gcacgtgacc caggcgctcg gtcttgcctt gctcgtcggt gatgtacttc accagctccg
     7681 cgaagtcgct cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg cgcacccccc
     7741 ggccgtttta gcggctaaaa aagtcatggc tctgccctcg ggcggaccac gcccatcatg
     7801 accttgccaa gctcgtcctg cttctcttcg atcttcgcca gcagggcgag gatcgtggca
     7861 tcaccgaacc gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag gccgcccagg
     7921 cggcccaggt cgccattgat gcgggccagc tcgcggacgt gctcatagtc cacgacgccc
     7981 gtgattttgt agccctggcc gacggccagc aggtaggccg acaggctcat gccggccgcc
     8041 gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg
     8101 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg
     8161 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
     8221 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
     8281 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
     8341 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
     8401 gcagcggaaa agcgctgctt ccctgctgtt ttgtggaata tctaccgact ggaaacaggc
     8461 aaatgcagga aattactgaa ctgaggggac aggcgagaga cgatgccaaa gagctacacc
     8521 gacgagctgg ccgagtgggt tgaatcccgc gcggccaaga agcgccggcg tgatgaggct
     8581 gcggttgcgt tcctggcggt gagggcggat gtcgaggcgg cgttagcgtc cggctatgcg
     8641 ctcgtcacca tttgggagca catgcgggaa acggggaagg tcaagttctc ctacgagacg
     8701 ttccgctcgc acgccaggcg gcacatcaag gccaagcccg ccgatgtgcc cgcaccgcag
     8761 gccaaggctg cggaacccgc gccggcaccc aagacgccgg agccacggcg gccgaagcag
     8821 gggggcaagg ctgaaaagcc ggcccccgct gcggccccga ccggcttcac cttcaaccca
     8881 acaccggaca aaaaggatcc agcagttcaa cctgttgata gtacgtacta agctctcatg
     8941 tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc
     9001 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
     9061 taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag
     9121 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa
     9181 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
     9241 caattttcag tgacacagga acacttaacg gctgacatgg ccttagaagg cgttttagaa
     9301 gccctaatgg atggagtcaa aacaaactag cgatc
//
