LOCUS       62056_22565        7040 bp DNA     circular SYN 07-OCT-2020
DEFINITION  Cloning vector pVG344_Aste_kh2-MCRv3-Vasa-Cas9_SWAP, complete 
            sequence.
ACCESSION   MW030448
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Adolfi A, Gantz V, Jasinskiene N, Lee H-F., Hwang K, Terradas G, 
            Bulger E, Ramaiah A, Bennett J, Emerson JJ, Marshall J, Bier E.
  TITLE     Efficient population modification gene-drive rescue system in the 
            malaria mosquito Anopheles stephensi
  JOURNAL   Nat Commun (2020) In press
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Adolfi A, Gantz V, Jasinskiene N, Lee H-F., Hwang K, Terradas G, 
            Bulger E, Ramaiah A, Bennett J, Emerson JJ, Marshall J, Bier E.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2020) Developmental and Stem Cell Biology, 
            University of California, San Francisco, 1650 Owens St., San 
            Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 7040)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-SEP-2020) Developmental and Stem Cell Biology, University of 
            California, San Francisco, 1650 Owens St., San Francisco, CA 94158, 
            USA"
FEATURES             Location/Qualifiers
     source          1..7040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(174..1031)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1032..1136)
                     /label=AmpR promoter
     misc_feature    1299..3187
                     /label=kynurenine hydroxylase
                     /note="kynurenine hydroxylase"
     misc_feature    2615..3090
                     /label=recoded kynurenine hydroxylase
                     /note="recoded kynurenine hydroxylase"
     misc_feature    3091..3290
                     /label=Anopheles gambiae genomic fragment
                     /note="Anopheles gambiae genomic fragment"
     3'UTR           3091..3187
                     /label=kynurenine hydroxylase 3'UTR
                     /note="kynurenine hydroxylase 3'UTR"
     misc_feature    3291..3340
                     /label=attP
                     /note="attP"
     polyA_signal    complement(3362..3483)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3594..3610
                     /label=gRNA SWAP1
                     /note="gRNA SWAP1"
     CDS             complement(3616..4332)
                     /label=EGFP
                     /note="enhanced GFP"
     regulatory      complement(4381..4603)
                     /label=3xP3 promoter
                     /note="3xP3 promoter"
                     /regulatory_class="promoter"
     regulatory      complement(4645..6327)
                     /label=Vasa promoter
                     /note="Vasa promoter"
                     /regulatory_class="promoter"
     rep_origin      complement(6452..7040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     1201 aaaaataggc gtatcacgag gccctttcgc atctgaacga ggtgctgcta aatggtacgt
     1261 ggctgtaagg gagagggtgt attgtgttac cgccagacgg atgcttttgt aaggcttcat
     1321 ttcacgcttg tttctgcttt tgcctttgca acagcggccg agaagtaccc gaacattcac
     1381 ctgcacttta accacaagct ggtcagcgcc aacctggacg agggcaatct gtcgatggtt
     1441 gagtaagtag aaaagtttgg gggtgtgttg ggggtgtgta aggaccgctt gactcactct
     1501 cgatcattcc ggagagtttt aagatctaaa caacctacga tctttagtga tcgcaaacac
     1561 aatcatgtga acatttggtt tgtagcatcc ttgagcttga tcctttgcac gtgttttgcc
     1621 acgaaacgat tggctcatct ttgcgataat actcagccac acttcttgat ggaccgtttc
     1681 gagcgagaca atcatatcaa atatcttttg caatattatg ggcgaaagat gtctctcgat
     1741 agctatggtt tgctgaaagc aatctaatca caaacacccg agacacttct ggacggaagc
     1801 ttggtccggc attcattcaa cgagcgagcg tgccgttgtc cgtcggccgc ttatcaccgt
     1861 ttgaagagtt gagccggagg aagcaatcct tcttcaacgg atttggttcg ttatcttcga
     1921 gaggagcgtt tttcccctcg gggatggcct gactcatcgc gttcgagtag cacgttgaag
     1981 ttgtgctggg aatgcctcac caggcgagtg tgaggattaa gcagacaagt ggcggaattc
     2041 cttgcacact cgagagaaac acgcgcgttc gttcaactgt tcgtctatcg ctaattcaac
     2101 tcccgcttta attctagtcc actaacgaaa gaggtcaaga gcgctcgggc agatctgatc
     2161 gtcggatgcg acggtgcgta cagtgccgtc cggaaggaga tcgtcaagcg tccccgctac
     2221 gactttagcc agacgtacat cgagcacggc tatctggagc tttgcatccc gccgacagcc
     2281 agcggagagt tcgccatgcc ccacaactat ctgcacatct ggccccgggg tcagtttatg
     2341 atgattgcgc tgccgaacca ggatcgcacc tggacggtga cactgttcat gcccttcaca
     2401 cagttccaca gtattaccga tccggggcgg ttgatcgatt tcttccgcca gtacttcccc
     2461 gatgcgatcg agctaattgg gcgcgaacgg ttgataaagg atttcttcaa gacgaagccc
     2521 caaccgctcg tcatgatcaa atgtcgaccg taccacgtgg gatcgaaggc attgatcatc
     2581 ggagatgcgg cgcacgcgat ggttccgttc tacggacaag gaatgaatgc aggattcgag
     2641 gattgctccg ttctcaccga tttgtttaat caatatggca ccgatttgac acgtatactc
     2701 ccggaattca gtgaaaagcg ctgggaagac gctcacgcta tctgcgatct ggctatgtat
     2761 aattacatcg aaatgcggga cttggtgacg aagcgttcgt atttgctcag gaaaaaattg
     2821 gatgaactct tgttttggct cctgcctaat acctgggtgc cgctctataa ttcggtctcg
     2881 tttagccaca tgcgatactc caaatgtatt gcaaatcgcg cttggcaaga caaaatactc
     2941 actcgagtct tgtatggtgc aagcttcgtc agcgtggccg cgataggtgg tttggcatat
     3001 cgccatatga cagttggtca cctggagcgt ctgagtagtg ctatactgag cacttttcag
     3061 ttgttgaagc ccaacacagc ttccgtgtaa ctgtgccccc cgtaccattc gtttattgct
     3121 aaaaaataaa acttatatac tcactcaaca tgttcacgtt tgcgatgttt taacgagttt
     3181 aatatttatt tgctttgtgt gtttggtttg tttctacaaa aaacaacgtg ttttcttcta
     3241 cacctgtttt tttatgttta tagtcgcatg caactagatt aattgtacaa gtagtgcccc
     3301 aactggggta acctttgagt tctctcagtt gggggcgtag cgtacgcgta tcgataagct
     3361 ttaagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3421 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3481 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     3541 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct agagtcgcgg
     3601 ccgctacagg ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
     3661 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
     3721 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
     3781 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
     3841 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
     3901 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
     3961 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
     4021 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
     4081 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag
     4141 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
     4201 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
     4261 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
     4321 cttgctcacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg actctagcgg
     4381 taccccgatt gtttagcttg ttcagctgcg cttgtttatt tgcttagctt tcgcttagcg
     4441 acgtgttcac tttgcttgtt tgaattgaat tgtcgctccg tagacgaagc gcctctattt
     4501 atactccggc ggtcgagggt tcgaaatcga taagcttgga tcctaattga attagctcta
     4561 attgaattag tctctaattg aattagatcc ccgggcgagc tcgaattaac cattgtggga
     4621 accgtgcgat caaacaactc gagcctggta ccacagtctt attggcgtga tgggttagtt
     4681 taggtaatac tcgacgcttt ataccaccta gcgcgagggt cgccggcaat actcaaacct
     4741 acacaaccgc gctaaagttt gcttttaact ttacagctcc gtttacaaat gcttaaaatt
     4801 atgagcttgt tacaccaatg tgtgttgttt acctgtcttt cgtttccgga tcagcctaac
     4861 gtgcagtgcc tgcagcccgc actggatgag atcgctgttg atctgcaaaa tgaaattaaa
     4921 ttataatcac tattctactc tagtttccaa catcggaata catacgtcag cttcagtgac
     4981 ctgtggggct agcccgcgaa tgatgatgtt attgttgggc ttttgttgat ggaaaaactc
     5041 attgtcactg tcaaaatttt ccccatcatc catcgagcta ggcgaatatc cgccacgacg
     5101 gccgccctta tcgcgatccc tgaaaaaaaa agcgattggc gatgaattga atgtaacaca
     5161 cggcaagcaa actggtctac taccttcgat cgcggtatcg atcccgttcg cgggatctac
     5221 gatcgcgatc ccgttcgcga ctgcgttcac gaaacctttc gcggctaaaa taaattttag
     5281 atagacaaat cactttaatc acgcatccca cactatgctt tgctgcaatc gtacaaacct
     5341 gtcccaaccg ccatcatcgt acgtttgttc gttcccatcc cggtacgagt cgcgttcggt
     5401 gctgcgctgc cggggatagg agtagcgctc cctgctgtac gaccgcgacc ggctgcggct
     5461 gtaccggctg ctattgcggt accgttccct aaaaggttta aaaaagcacc aattaatttc
     5521 cggacacggt ttcgtgcaca cggtggggtg ttccggcaca ccggtgtggc ggcgagacac
     5581 ggaacactac cactggagac catgctacga taaggcccaa ggcgagacaa cggcaccagc
     5641 ggacggcact cttcgcaacg gcgtgtctct gggtagggag gacgcatttg ggcacgcatg
     5701 tactccgggg aggggtgcta tacggtcaag cgcttacttc gatcttcctc gattgccccg
     5761 gtcgcgctca tcacgtcggc agcgatttct atagtagcca gaagtatcac tgtctggact
     5821 cggtgagact ataaacaatc acatttcaaa ggaaattatt ggtcggtcga tcgaaaacat
     5881 gggaagaatt agggcgaaca ttggttcatg caatcttcgc ccgtcgcttt ctgtatgagg
     5941 gtggtctatt ataactttca gatataaaat aactcggctg tttttttgct tttttttttg
     6001 tcactaccct ccagtaccta tctagaacac ccagccaggt agaggtgaca agatagaaca
     6061 taaaatgctt ttgcctacag aagtggacag actgactgaa tgaaggtagc ctaaaacgca
     6121 aagaaaaact ctcagctttt cggtggtgta cgctaggccg gctggtttgt tatccattcg
     6181 cctttccaat cgaaccgtag cgtacacggg aaaacacaca aaaccaaacc aaaactctac
     6241 tacactttca aagatggcag acttacaatc catctcggtt gccttgaaac gctccagccc
     6301 gaatgcgccg tctccttttg ctcacggtat cagctcactc aaaggcggta atacggttat
     6361 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7021 gcagaaaaaa aggatctcaa
//
