LOCUS       V015639                 6217 bp    DNA     circular SYN 31-JUL-2019
DEFINITION  Exported.
ACCESSION   V015639
VERSION     V015639
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Vezina B, Moore RJ.
  TITLE     Bioinformatic analysis and development of molecular tools for
            broiler chickens live Lactobacillis vaccine vector candidate
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   Vezina B, Moore RJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2019) Microbiology, Monash University, Monash
            University, Clayton Campus, Wellington Rd, Clayton, VIC 3800,
            Australia
REFERENCE   3  (bases 1 to 6217)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-JAN-2019) Microbiology, Monash University, Monash University,
            Clayton Campus, Wellington Rd, Clayton, VIC 3800, Australia"
FEATURES             Location/Qualifiers
     source          1..6217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             101..814
                     /codon_start=1
                     /transl_table=11
                     /product="Rep pLa3_4"
                     /label="Rep pLa3_4"
                     /note="Rep protein"
                     /protein_id="QDQ71306.1"
                     /translation="MPEKKKDNRARTWTFIVYPESVPNNWRELLDSYHIPWVESPLHDK
                     DVNPDGTIKKPHWHVILMFDGKKSYEQIKEITDSLNAPVPQKTANPKGLVRYLIHMDNP
                     EKYQYKKEDIVCHSGAEVEQYFQLSSASRLQILKEMIIFIKDSRFENYLDFLAYCIENG
                     EDEWLDIAVNHNTLALSKVIDSVYQKNHPKTPQTDYKQTVVAKAKEMARQGTSQRVIAD
                     TLGISPATVNRYLKK"
     CDS             1587..1901
                     /codon_start=1
                     /transl_table=11
                     /product="ORF-2"
                     /label="ORF-2"
                     /protein_id="QDQ71307.1"
                     /translation="MLFLTNFRQKARLNSRYPTSCVLWRGPTWFLLTSPWSDSKGKVRR
                     SFFGSFFVASNENKNSFCKNDLGFLNFKNYFCENKNKKARSKNTVRFFIFDFGFEENIF
                     "
     CDS             complement(1957..2115)
                     /codon_start=1
                     /transl_table=11
                     /product="ORF-3"
                     /label="ORF-3"
                     /protein_id="QDQ71308.1"
                     /translation="MIYFLWLSNQSLSKNKKIPTKSGAVTAISKEFIPSPPFQLAKGHS
                     NLSIISR"
     CDS             2361..2981
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     promoter        complement(3342..3360)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3378..3394)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3402..3418)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3426..3456)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3471..3492)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3780..4368)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4542..5399)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5400..5504)
                     /label="AmpR promoter"
     rep_origin      complement(5582..6037)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6178..6194
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6201..6217
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 ctacttccgg ctgtaagttg gcttgtagga ggtttttatt atgcctgaaa agaaaaaaga
      121 taatcgagca agaacttgga cttttattgt atatccggaa agcgtcccaa ataactggcg
      181 tgaactttta gatagctatc atattccttg ggttgagagt ccgttacatg ataaagatgt
      241 caaccccgat ggaacgatca aaaagcctca ctggcatgtt attttgatgt ttgacggtaa
      301 gaaatcgtat gagcaaataa aagagattac ggatagtctt aatgcgcctg taccgcagaa
      361 aacagctaat ccaaaggggt tagttagata tttaattcat atggacaacc ccgaaaagta
      421 tcaatacaag aaagaggata tagtctgtca tagtggggct gaggtggaac aatatttcca
      481 gctttctagt gcgtcacgac ttcaaatttt gaaagagatg attattttta ttaaggatag
      541 tcgatttgaa aactacttag attttttggc ttattgtatt gaaaatggag aagatgagtg
      601 gcttgatata gctgtaaatc ataatacttt agctctttct aaggtgattg attctgttta
      661 ccaaaaaaat caccctaaaa ccccgcagac tgactataaa caaactgtgg ttgcaaaagc
      721 aaaagaaatg gcaaggcaag ggacttcgca aagagttatt gcagatactt taggaattag
      781 tcctgcaacc gttaatcgtt atttgaagaa ataaaaaaga ccccaaaatg gggtcttttt
      841 ttgagtgttt catttttttt gtttcatttt gaaacgctga aacaaaaagt gaaacgcctt
      901 tgaagccttg tggctgtaag ggttagcagg tgtttcattg gggggtacta cgacaccccc
      961 caatgaggtc gttggtcatt agtcaaaaat tagtgagaat gagaactcga ttgagcgtag
     1021 cgaaactcga gttctcattc gagagttaag cgcgattgag caaagcgaaa ctcgcagctt
     1081 aacgggcacg gagtgcttgc tcgtcagagc aaaaaattag aatttgattt tttaagtcca
     1141 agttaactta gattttcaaa ttttattttg gcatttttga aactaaaaaa gtggtggtta
     1201 gcccgcttgt gaaaaaagtt caaatctatt ttggcgtttt ttgaatttca aaactaaact
     1261 caaattttca aaaaagaggg gcggtttttg ctcctgtttt tttattattt tggacaccaa
     1321 aaattcatgg gagggccata taaatagagg gccctattat ggggaccccc ctatcaatgg
     1381 gggggcatgt aaatagaggg cctacttcat ggagggtcat atttaaaggg ggccaggccg
     1441 atagggggac ctattatagg aggtccaggt caatagaggg ccctatcaat agggggacct
     1501 aaaaaagtag gcctagccga gaaaattagt atcacggaaa ccccccgcac tgcggggccg
     1561 ccaagacttt ggcggttgtt tctcttatgc tctttttgac gaattttcgg caaaaagccc
     1621 gcctaaatag cagatacccc actagctgcg tcctgtggcg aggaccgact tggtttttgc
     1681 ttacttcgcc ttggtcggac tccaagggaa aagtaaggcg ctcctttttt ggttcttttt
     1741 tcgttgcgag taacgaaaat aagaactcgt tttgcaaaaa cgatctaggc tttttgaatt
     1801 ttaaaaatta cttttgcgaa aataaaaata aaaaagcgcg gtcaaaaaat accgtgcgtt
     1861 tttttatttt tgattttggt tttgaggaaa atatttttta gacgagtaat ataaaatttg
     1921 taagtatata ttttggtttg cgatgaatta agggaattag cgtgatataa tactcaagtt
     1981 gctatgtccc tttgccaatt ggaaaggggg tgaggggatg aattcttttg atatcgcagt
     2041 cactgcgcca ctttttgttg gtatcttttt gtttttgctt aaagattggt tagacagcca
     2101 caaaaagtaa atcatggcaa caatgatcta gccactaagt cgagctttca ggcgagttcg
     2161 gctttttttg cgcataaaaa aagccgtgtt tatcaggcgc acacgacatc actagttatt
     2221 agaattcttt ttcggcaagt gttcaagaag ttattaagtc gggagtgcag tcgaagtggg
     2281 caagttgaaa aattcacaaa aatgtggtat aatatctttg ttcattagag cgataaactt
     2341 gaatttgaga gggaacttag atggtatttg aaaaaattga taaaaatagt tggaacagaa
     2401 aagagtattt tgaccactac tttgcaagtg taccttgtac atacagcatg accgttaaag
     2461 tggatatcac acaaataaag gaaaagggaa tgaaactata tcctgcaatg ctttattata
     2521 ttgcaatgat tgtaaaccgc cattcagagt ttaggacggc aatcaatcaa gatggtgaat
     2581 tggggatata tgatgagatg ataccaagct atacaatatt tcacaatgat actgaaacat
     2641 tttccagcct ttggactgag tgtaagtctg actttaaatc atttttagca gattatgaaa
     2701 gtgatacgca acggtatgga aacaatcata gaatggaagg aaagccaaat gctccggaaa
     2761 acatttttaa tgtatctatg ataccgtggt caaccttcga tggctttaat ctgaatttgc
     2821 agaaaggata tgattatttg attcctattt ttactatggg gaaatattat aaagaagata
     2881 acaaaattat acttcctttg gcaattcaag ttcatcacgc agtatgtgac ggatttcaca
     2941 tttgccgttt tgtaaacgaa ttgcaggaat tgataaatag ttaacttcag gtttgtctgt
     3001 aactaaaaac aagtatttaa gcaaaaacat cgtagaaata cggtgttttt tgttacccta
     3061 aaatctacaa ttttatacat aaccacaggt tagtacaaag accttgtgtt tctttttgaa
     3121 aggcttaaaa caaggatttt tccttgattt aagccccgaa aagcaacaca accaaggttt
     3181 tagtatcaat ctgtgctagc aaatggatcc gatatcgcat gcgccggcaa gaaaaaaaag
     3241 cccgctcatt aggcgggcct gcagaactag tgaattcgcg gccgcctgca ggtcgaccat
     3301 atgggagagc tcccaacgcg ttggatgcat agcttgagta ttctatagtg tcacctaaat
     3361 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3421 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3481 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3541 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3601 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3661 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3721 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3781 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3841 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3901 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3961 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4021 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4081 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4141 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4201 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4261 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4321 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4381 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4441 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4501 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4561 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4621 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4681 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4741 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4801 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4861 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4921 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4981 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5041 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5101 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5161 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5221 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5281 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5341 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5401 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5461 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5521 gtgccacctg atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     5581 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     5641 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     5701 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     5761 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     5821 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     5881 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     5941 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     6001 acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca
     6061 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     6121 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     6181 aaacgacggc cagtgaattg taatacgact cactata
//
