LOCUS       V002248                 4061 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002248
VERSION     V002248
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Gupta A, Onda M, Pastan I, Adhya S, Chaudhary VK.
  TITLE     High-density functional display of proteins on bacteriophage lambda
  JOURNAL   J. Mol. Biol. 334 (2), 241-254 (2003)
   PUBMED   14607116
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Gupta A, Chaudhary VK.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2002) Biochemistry, University of Delhi South
            Campus, Benito Juarez Road, New Delhi 110021, India
REFERENCE   3  (bases 1 to 4061)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4061)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Mol.
            Biol."; date: "2003"; volume: "334"; issue: "2"; pages: "241-254"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-DEC-2002) Biochemistry, University of Delhi South Campus, Benito
            Juarez Road, New Delhi 110021, India"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             265..594
                     /gene="D"
                     /label="Capsid decoration protein"
                     /note="Capsid decoration protein from Escherichia phage
                     lambda. Accession#: P03712"
     protein_bind    763..784
                     /label="CAP binding site"
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        799..829
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    837..853
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             873..1046
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
     protein_bind    1346..1379
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      1379..1807
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     protein_bind    complement(1825..1858)
                     /label="loxP511"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     promoter        2156..2260
                     /label="AmpR promoter"
     CDS             2261..3118
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3292..3880
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 ggtctagata aggaggaata acatatgact tcgaaagaaa cctttaccca ttaccagccg
      301 cagggcaaca gtgacccggc tcataccgca accgcgcccg gcggattgag tgcgaaagcg
      361 cctgcaatga ccccgctgat gctggacacc tccagccgta agctggttgc gtgggatggc
      421 accaccgacg gtgctgccgt tggcattctt gcggttgctg ctgaccagac cagcaccacg
      481 ctgacgttct acaagtccgg cacgttccgt tatgaggatg tgctctggcc ggaggctgcc
      541 agcgacgaga cgaaaaaacg gaccgcgttt gccggaacgg caatctcaat tgttggcgga
      601 tccggaccgg tagggcccgg tggcagcgga gctagcaacg cgtcgcccgg gcagtaagcg
      661 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      721 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      781 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      841 gagcggataa caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg
      901 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
      961 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     1021 aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg
     1081 tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa
     1141 actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat cccattacgg
     1201 tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg
     1261 ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc gtttaagccc
     1321 gggcgcgcta attaattaag aattcataac ttcgtataat gtatgctata cgaagttatc
     1381 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1441 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     1501 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     1561 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     1621 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     1681 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     1741 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     1801 gcgaatttta acaaaatatt aacgataact tcgtatagta tacattatac gaagttatga
     1861 gctctttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     1921 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     1981 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     2041 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2101 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2161 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2221 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2281 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2341 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2401 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     2461 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     2521 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     2581 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     2641 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     2701 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2761 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     2821 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2881 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2941 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3001 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3061 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3121 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3181 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3241 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3301 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3361 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3421 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3481 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3541 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3601 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3661 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3721 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3781 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3841 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3901 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3961 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     4021 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa g
//
