LOCUS       40924_45898       11224 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVC4-bar, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   Conn SJ, Conn V, Tyerman SD, Kaiser BN, Leigh RA, Gilliham M.
  TITLE     Magnesium transporters, MGT2/MRS2-1 and MGT3/MRS2-5, are important 
            for magnesium partitioning within Arabidopsis thaliana mesophyll 
            vacuoles
  JOURNAL   New Phytol. 190 (3), 583-594 (2011)
  PUBMED    21261624
REFERENCE   2  (bases 1 to 11224)
  AUTHORS   Conn V.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 11224)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11224)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New 
            Phytol."; date: "2011"; volume: "190"; issue: "3"; pages: "583-594"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-AUG-2010) Agriculture, Food "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(82..106)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        526..870
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1078..1094
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1175..1299
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1324..1354
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1408..2064
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2409..2711
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2755..2879)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2912..3159
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3191..3207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3215..3231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3239..3269)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3284..3305)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3496..4173
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             4218..4766
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    4776..4950
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(5028..5052)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5477..6268
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6358..6946
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7132..7272)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7616..7810)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7879..8943)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(9380..10006)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgttattcta
       61 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
      121 ggtcaacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc
      181 agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg
      241 attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc
      301 ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag
      361 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
      421 cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca cacttgtcta
      481 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca
      541 aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt
      601 gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
      661 catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
      721 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
      781 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
      841 ataaggaagt tcatttcatt tggagaggac ctcgactcta gaggatccca aactgaaggc
      901 gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca
      961 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     1021 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     1081 aaacgacggc cagtgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta
     1141 ccgggccccc cctcgaggcg cgccaagcta tcaaacaagt ttgtacaaaa aagctgaacg
     1201 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
     1261 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca
     1321 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
     1381 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
     1441 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
     1501 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
     1561 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     1621 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
     1681 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
     1741 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     1801 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1861 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1921 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1981 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     2041 tgcgatgagt ggcagggcgg ggcgtaatct agaggatccg gcttactaaa agccagataa
     2101 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     2161 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     2221 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     2281 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     2341 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     2401 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     2461 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     2521 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     2581 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     2641 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     2701 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     2761 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     2821 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2881 cgataattcc ttattaatta agaatttccc cgatcgttca aacatttggc aataaagttt
     2941 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     3001 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     3061 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     3121 ctaggataaa ttatcgcgcg cggtgtcatc tatgttactc accgcggtgg agctcgaatt
     3181 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     3241 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     3301 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     3361 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggctag agcagcttgc
     3421 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
     3481 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
     3541 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
     3601 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
     3661 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
     3721 gtcttcaaag caagtggatt gatgtgataa catggtggag cacgacactc tcgtctactc
     3781 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
     3841 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
     3901 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
     3961 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     4021 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     4081 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccttc ctctatataa
     4141 ggaagttcat ttcatttgga gaggacacgc tgaaatcacc agtctctctc tacaaatcta
     4201 tctctctcga gtctaccatg agcccagaac gacgcccggc cgacatccgc cgtgccaccg
     4261 aggcggacat gccggcggtc tgcaccatcg tcaaccacta catcgagaca agcacggtca
     4321 acttccgtac cgagccgcag gaaccgcagg agtggacgga cgacctcgtc cgtctgcggg
     4381 agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt cgccggcatc gcctacgcgg
     4441 gcccctggaa ggcacgcaac gcctacgact ggacggccga gtcgaccgtg tacgtctccc
     4501 cccgccacca gcggacggga ctgggctcca cgctctacac ccacctgctg aagtccctgg
     4561 aggcacaggg cttcaagagc gtggtcgctg tcatcgggct gcccaacgac ccgagcgtgc
     4621 gcatgcacga ggcgctcgga tatgcccccc gcggcatgct gcgggcggcc ggcttcaagc
     4681 acgggaactg gcatgacgtg ggtttctggc agctggactt cagcctgccg gtaccgcccc
     4741 gtccggtcct gcccgtcacc gagatttgac tcgagtttct ccataataat gtgtgagtag
     4801 ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga
     4861 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     4921 aaaccaaaat ccagtactaa aatccagatc ccccgaatta attcggcgtt aattcagtac
     4981 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     5041 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     5101 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     5161 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     5221 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     5281 cctgtgatca ccgcggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg
     5341 aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg
     5401 aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag
     5461 aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga
     5521 aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt
     5581 gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta
     5641 tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa
     5701 ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg
     5761 cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta
     5821 tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag
     5881 cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga
     5941 ttgcgaaaac tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt
     6001 aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa
     6061 catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc
     6121 ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga
     6181 acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa
     6241 atattatatt ttactggatg aattgtttta gtacctagaa tgcatgacca aaatccctta
     6301 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6361 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6421 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6481 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6541 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6601 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6661 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6721 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6781 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6841 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6901 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6961 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7021 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7081 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     7141 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     7201 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     7261 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     7321 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     7381 ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc
     7441 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     7501 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     7561 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     7621 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     7681 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     7741 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     7801 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     7861 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     7921 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     7981 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     8041 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     8101 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     8161 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     8221 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     8281 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     8341 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     8401 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     8461 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     8521 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     8581 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     8641 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
     8701 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
     8761 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
     8821 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
     8881 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
     8941 accgtcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
     9001 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
     9061 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
     9121 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
     9181 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
     9241 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
     9301 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
     9361 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
     9421 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
     9481 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
     9541 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
     9601 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
     9661 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
     9721 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
     9781 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
     9841 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
     9901 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
     9961 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    10021 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    10081 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    10141 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    10201 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    10261 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    10321 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    10381 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    10441 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    10501 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    10561 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    10621 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    10681 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    10741 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    10801 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    10861 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    10921 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    10981 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    11041 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    11101 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    11161 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
    11221 catg
//
