LOCUS       62056_22515        6050 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        36..415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        416..619
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        664..682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             785..3829
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPVPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     polyA_signal    3880..4104
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             4277..5068
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      5397..5985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
       61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      241 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      421 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      541 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      601 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
      661 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt
      721 accgagctcg gatccactag tccagtgtgg tggaattctg cagatcgaaa cgatgataga
      781 tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      841 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      901 ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggtaccaga
      961 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc
     1021 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat
     1081 tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt
     1141 taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa
     1201 ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt
     1261 gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat
     1321 ggtgctgcgt tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg
     1381 cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt
     1441 tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg
     1501 cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt
     1561 cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga
     1621 tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa
     1681 tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc
     1741 ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa
     1801 gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat
     1861 ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt
     1921 gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct
     1981 gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac
     2041 cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga
     2101 tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa
     2161 tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga
     2221 aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga
     2281 tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc
     2341 tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg
     2401 cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt
     2461 ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc
     2521 ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt
     2581 ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt
     2641 ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag
     2701 cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga
     2761 agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca
     2821 gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc
     2881 atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag
     2941 tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt
     3001 ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca
     3061 gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc
     3121 accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt
     3181 cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc
     3241 agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa
     3301 aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac
     3361 cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca
     3421 gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc
     3481 cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac
     3541 cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg
     3601 cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact
     3661 gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga
     3721 cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt
     3781 ccagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaag cggccgctcg
     3841 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     3901 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3961 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4021 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4081 ctggggatgc ggtgggctct atggcttcta ctgggcggtt ttatggacag caagcgaacc
     4141 ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat
     4201 ggctttctcg ccgccaagga tctgatggcg caggggatca agctctgatc aagagacagg
     4261 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4321 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4381 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4441 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4501 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4561 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4621 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4681 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4741 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4801 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4861 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4921 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4981 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5041 cttctatcgc cttcttgacg agttcttctg aattattaac gcttacaatt tcctgatgcg
     5101 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacaggtgg cacttttcgg
     5161 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5221 ctcatgagac aataaccctg ataaatgctt caataatagc acgtgctaaa acttcatttt
     5281 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5341 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5401 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5461 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5521 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5581 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5641 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5701 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5761 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5821 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5881 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5941 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6001 gcctttttac ggttcctggg cttttgctgg ccttttgctc acatgttctt
//
