LOCUS       V015862                 4263 bp    DNA     circular SYN 01-MAY-2019
DEFINITION  Exported.
ACCESSION   V015862
VERSION     V015862
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Feichtinger GA.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2019) Division of Oral Biology, University of
            Leeds, Wellcome Trust Brenner Building, Level 7, St. James
            University Hospital Campus, Leeds, West Yorkshire LS9 7TF, United
            Kingdom
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (22-JAN-2019) Division of Oral Biology, University of Leeds,
            Wellcome Trust Brenner Building, Level 7, St. James University
            Hospital Campus, Leeds, West Yorkshire LS9 7TF, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..4263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        36..415
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        416..619
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        664..682
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      764..769
                     /label="Kozak sequence"
                     /note="Kozak sequence"
                     /regulatory_class="other"
     CDS             770..1957
                     /gene="BMP2"
                     /label="Bone morphogenetic protein 2"
                     /note="Bone morphogenetic protein 2 from Homo sapiens.
                     Accession#: P12643"
     polyA_signal    2042..2266
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     CDS             2439..3230
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3558..4146
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
       61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      241 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      421 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      541 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      601 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
      661 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt
      721 accgagctcg gatccactag tccagtgtgg tggaattcgg cttgccacca tggtggccgg
      781 gacccgctgt cttctagcgt tgctgcttcc ccaggtcctc ctgggcggcg cggctggcct
      841 cgttccggag ctgggccgca ggaagttcgc ggcggcgtcg tcgggccgcc cctcatccca
      901 gccctctgac gaggtcctga gcgagttcga gctgcggctg ctcagcatgt tcggcctgaa
      961 acagagaccc acccccagca gggacgccgt ggtgcccccc tacatgctag acctgtatcg
     1021 caggcactca ggtcagccgg gctcacccgc cccagaccac cggttggaga gggcagccag
     1081 ccgagccaac actgtgcgca gcttccacca tgaagaatct ttggaagaac taccagaaac
     1141 gagtgggaaa acaacccgga gattcttctt taatttaagt tctatcccca cggaggagtt
     1201 tatcacctca gcagagcttc aggttttccg agaacagatg caagatgctt taggaaacaa
     1261 tagcagtttc catcaccgaa ttaatattta tgaaatcata aaacctgcaa cagccaactc
     1321 gaaattcccc gtgaccagac ttttggacac caggttggtg aatcagaatg caagcaggtg
     1381 ggaaagtctt gatgtcaccc ccgctgtgat gcggtggact gcacagggac acgccaacca
     1441 tggattcgtg gtggaagtgg cccacttgga ggagaaacaa ggtgtctcca agagacatgt
     1501 taggataagc aggtctttgc accaagatga acacagctgg tcacagataa ggccattgct
     1561 agtaactttt ggccatgatg gaaaagggca tcctctccac aaaagagaaa aacgtcaagc
     1621 caaacacaaa cagcggaaac gccttaagtc cagctgtaag agacaccctt tgtacgtgga
     1681 cttcagtgac gtggggtgga atgactggat tgtggctccc ccggggtatc acgcctttta
     1741 ctgccacgga gaatgccctt ttcctctggc tgatcatctg aactccacta atcatgccat
     1801 tgttcagacg ttggtcaact ctgttaactc taagattcct aaggcatgct gtgtcccgac
     1861 agaactcagt gctatctcga tgctgtacct tgacgagaat gaaaaggttg tattaaagaa
     1921 ctatcaggac atggttgtgg agggttgtgg gtgtcgctag aagccgaatt ctgcagatat
     1981 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     2041 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2101 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     2161 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     2221 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tactgggcgg
     2281 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
     2341 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat
     2401 caagctctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     2461 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     2521 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     2581 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     2641 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     2701 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     2761 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     2821 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     2881 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     2941 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     3001 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     3061 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     3121 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     3181 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattatta
     3241 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3301 gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3361 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatag
     3421 cacgtgctaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3481 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3541 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3601 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3661 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3721 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3781 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3841 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3901 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3961 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4021 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4081 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4141 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4201 cacatgttct tgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     4261 act
//
