LOCUS       V016550                 6649 bp    DNA     circular SYN 01-MAY-2019
DEFINITION  Exported.
ACCESSION   V016550
VERSION     V016550
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Feichtinger GA.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2019) Division of Oral Biology, University of
            Leeds, Wellcome Trust Brenner Building, Level 7, St. James
            University Hospital Campus, Leeds, West Yorkshire LS9 7TF, United
            Kingdom
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (22-JAN-2019) Division of Oral Biology, University of Leeds,
            Wellcome Trust Brenner Building, Level 7, St. James University
            Hospital Campus, Leeds, West Yorkshire LS9 7TF, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..6649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        36..413
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        416..619
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        664..682
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             770..1957
                     /gene="BMP2"
                     /label="Bone morphogenetic protein 2"
                     /note="Bone morphogenetic protein 2 from Homo sapiens.
                     Accession#: P12643"
     polyA_signal    2042..2266
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     CDS             2439..3230
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     enhancer        3415..3794
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3796..3995
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     regulatory      4043..4061
                     /label="T7 promoter"
                     /note="T7 promoter"
                     /regulatory_class="promoter"
     CDS             4106..5398
                     /gene="BMP7"
                     /label="Bone morphogenetic protein 7"
                     /note="Bone morphogenetic protein 7 from Homo sapiens.
                     Accession#: P18075"
     misc_feature    5608..5832
                     /label="bGH poly(A) signal"
                     /note="bGH poly(A) signal"
     rep_origin      5944..6532
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
       61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      241 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      421 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      541 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      601 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
      661 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt
      721 accgagctcg gatccactag tccagtgtgg tggaattcgg cttgccacca tggtggccgg
      781 gacccgctgt cttctagcgt tgctgcttcc ccaggtcctc ctgggcggcg cggctggcct
      841 cgttccggag ctgggccgca ggaagttcgc ggcggcgtcg tcgggccgcc cctcatccca
      901 gccctctgac gaggtcctga gcgagttcga gctgcggctg ctcagcatgt tcggcctgaa
      961 acagagaccc acccccagca gggacgccgt ggtgcccccc tacatgctag acctgtatcg
     1021 caggcactca ggtcagccgg gctcacccgc cccagaccac cggttggaga gggcagccag
     1081 ccgagccaac actgtgcgca gcttccacca tgaagaatct ttggaagaac taccagaaac
     1141 gagtgggaaa acaacccgga gattcttctt taatttaagt tctatcccca cggaggagtt
     1201 tatcacctca gcagagcttc aggttttccg agaacagatg caagatgctt taggaaacaa
     1261 tagcagtttc catcaccgaa ttaatattta tgaaatcata aaacctgcaa cagccaactc
     1321 gaaattcccc gtgaccagac ttttggacac caggttggtg aatcagaatg caagcaggtg
     1381 ggaaagtctt gatgtcaccc ccgctgtgat gcggtggact gcacagggac acgccaacca
     1441 tggattcgtg gtggaagtgg cccacttgga ggagaaacaa ggtgtctcca agagacatgt
     1501 taggataagc aggtctttgc accaagatga acacagctgg tcacagataa ggccattgct
     1561 agtaactttt ggccatgatg gaaaagggca tcctctccac aaaagagaaa aacgtcaagc
     1621 caaacacaaa cagcggaaac gccttaagtc cagctgtaag agacaccctt tgtacgtgga
     1681 cttcagtgac gtggggtgga atgactggat tgtggctccc ccggggtatc acgcctttta
     1741 ctgccacgga gaatgccctt ttcctctggc tgatcatctg aactccacta atcatgccat
     1801 tgttcagacg ttggtcaact ctgttaactc taagattcct aaggcatgct gtgtcccgac
     1861 agaactcagt gctatctcga tgctgtacct tgacgagaat gaaaaggttg tattaaagaa
     1921 ctatcaggac atggttgtgg agggttgtgg gtgtcgctag aagccgaatt ctgcagatat
     1981 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     2041 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2101 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     2161 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     2221 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tactgggcgg
     2281 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
     2341 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat
     2401 caagctctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     2461 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     2521 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     2581 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     2641 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     2701 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     2761 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     2821 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     2881 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     2941 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     3001 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     3061 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     3121 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     3181 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattatta
     3241 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3301 gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3361 atacattcaa atatgtatcc gctcatgtcg atgtacgggc cagatatacg cgttgacatt
     3421 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     3481 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     3541 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     3601 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     3661 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     3721 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     3781 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     3841 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     3901 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3961 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
     4021 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
     4081 gtttaaactt aagcttgcca ccgcgatgca cgtgcgctca ctgcgagctg cggcgccgca
     4141 cagcttcgtg gcgctctggg cacccctgtt cctgctgcgc tccgccctgg ccgacttcag
     4201 cctggacaac gaggtgcact cgagcttcat ccaccggcgc ctccgcagcc aggagcggcg
     4261 ggagatgcag cgcgagatcc tctccatttt gggcttgccc caccgcccgc gcccgcacct
     4321 ccagggcaag cacaactcgg cacccatgtt catgctggac ctgtacaacg ccatggcggt
     4381 ggaggagggc ggcgggcccg gcggccaggg cttctcctac ccctacaagg ccgtcttcag
     4441 tacccagggc ccccctctgg ccagcctgca agatagccat ttcctcaccg acgccgacat
     4501 ggtcatgagc ttcgtcaacc tcgtggaaca tgacaaggaa ttcttccacc cacgctacca
     4561 ccatcgagag ttccggtttg atctttccaa gatcccagaa ggggaagctg tcacggcagc
     4621 cgaattccgg atctacaagg actacatccg ggaacgcttc gacaatgaga cgttccggat
     4681 cagcgtttat caggtgctcc aggagcactt gggcagggaa tcggatctct tcctgctcga
     4741 cagccgtacc ctctgggcct cggaggaggg ctggctggtg tttgacatca cagccaccag
     4801 caaccactgg gtggtcaatc cgcggcacaa cctgggcctg cagctctcgg tggagacgct
     4861 ggatgggcag agcatcaacc ccaagttggc gggcctgatt gggcggcacg ggccccagaa
     4921 caagcagccc ttcatggtgg ctttcttcaa ggccacggag gtccacttcc gcagcatccg
     4981 gtccacgggg agcaaacagc gcagccagaa ccgctccaag acgcccaaga accaggaagc
     5041 cctgcggatg gccaacgtgg cagagaacag cagcagcgac cagaggcagg cctgtaagaa
     5101 gcacgagctg tatgtcagct tccgagacct gggctggcag gactggatca tcgcgcctga
     5161 aggctacgcc gcctactact gtgaggggga gtgtgccttc cctctgaact cctacatgaa
     5221 cgccaccaac cacgccatcg tgcagacgct ggtccacttc atcaacccgg aaacggtgcc
     5281 caagccctgc tgtgcgccca cgcagctcaa tgccatctcc gtcctctact tcgatgacag
     5341 ctccaacgtc atcctgaaga aatacagaaa catggtggtc cgggcctgtg gctgccacta
     5401 gctcctccga gaattcagac cctttggggc caagtttttc tggatcctcc attgctcgcc
     5461 ttggccagga accagcagac caactgcctt ttgtgagacc ttcccctccc tatccccaac
     5521 tttaaaggtg tgagagtatt aggaaacatg agcagcagcg gccgctcgag tctagagggc
     5581 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5641 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     5701 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5761 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     5821 tgggctctat ggcttctact gggcggtttt atggacagca agcgaacaca tgaccaaaat
     5881 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6061 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6541 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttcttgct
     6601 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgact
//
