LOCUS       62056_22465        5454 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        35..414
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        519..618
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     CDS             643..696
                     /codon_start=1
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
                     /translation="EGRGSLLTCGDVEENPGP"
     enhancer        719..1096
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1099..1302
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        1347..1365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1394..1447
                     /codon_start=1
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    1629..1853
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1963..2292
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2341..3363
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    3496..3617
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             3681..4472
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      4801..5389
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gactcttcgc gatgtacggg ccagatatac gcgtgacatt gattattgac tagttattaa
       61 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      121 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      181 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggac
      241 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      301 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      361 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      421 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      481 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      541 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      601 gtctatataa gcagagctct ctggcgtcga ctagcaagat ctgagggcag aggaagtctt
      661 ctaacatgcg gtgacgtgga ggagaatccc ggccctgcgg ccgctacgac cccggggtga
      721 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      781 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      841 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      901 ttccattgac gtcaatgggt ggactattta cggtaaactg cccacttggc agtacatcaa
      961 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     1021 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     1081 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     1141 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     1201 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     1261 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
     1321 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
     1381 tagcatggat atcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
     1441 cggccctgac aagcttggta ccgagggcag aggaagtctt ctaacatgcg gtgacgtgga
     1501 ggagaatccc ggccctgagc tcggatcctc actcgaggag ggcagaggaa gtcttctaac
     1561 atgcggtgac gtggaggaga atcccggccc ttctagaggg cccgtttaaa cccgctgatc
     1621 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     1681 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     1741 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     1801 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctac
     1861 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
     1921 gttgggaagc cctgcaaagt aaactggatg gctttctcgc cggtgtgtca gttagggtgt
     1981 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2041 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2101 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     2161 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2221 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2281 ggcttttgca aaaagctctc gggagcttgt atatccattt tcggatctga tcagcacgtg
     2341 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     2401 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     2461 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     2521 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     2581 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     2641 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     2701 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     2761 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     2821 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     2881 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     2941 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     3001 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     3061 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     3121 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     3181 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     3241 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     3301 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     3361 gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     3421 ggaatcgttt tccgggacgc tggctggatg atcctccagc gcggggatct catgctggag
     3481 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3541 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3601 ctcatcaatg tatcttacca aggatctgat ggcgcagggg atcaagctct gatcaagaga
     3661 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     3721 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     3781 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     3841 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     3901 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     3961 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     4021 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     4081 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     4141 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     4201 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     4261 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4321 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4381 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4441 tcgccttcta tcgccttctt gacgagttct tctgaattat taacgcttac aatttcctga
     4501 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacag gtggcacttt
     4561 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4621 tccgctcatg agacaataac cctgataaat gcttcaataa tagcacgtgc taaaacttca
     4681 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4741 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4801 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4861 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4921 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4981 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5041 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5101 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5161 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5221 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5281 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5341 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5401 cgcggccttt ttacggttcc tgggcttttg ctggcctttt gctcacatgt tctt
//
