LOCUS       40924_45883        9240 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Overexpression vector pVALIUM10-roe, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Ni J-Q., Liu L-P., Perkins LA, Perrimon N.
  TITLE     Vectors for transgenic flies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9240)
  AUTHORS   Ni J-Q., Liu L-P., Perkins LA, Perrimon N.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2010) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 9240)
  AUTHORS   Hu Y, Perkins LA.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2012) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   4  (bases 1 to 9240)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9240)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-FEB-2010) Genetics, Harvard Medical School, 77 Avenue Louis 
            Pasteur, Boston, MA 02115, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (20-JUN-2012) Genetics, Harvard Medical School, 77 Avenue Louis 
            Pasteur, Boston, MA 02115, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Jun 20, 2012 this sequence version replaced GU931386.1.
FEATURES             Location/Qualifiers
     source          1..9240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    664..2543
                     /label=vermillion
                     /note="vermillion"
     CDS             complement(join(834..951,1028..1121,1213..1819,1874..2007,
                     2068..2228,2302..2327))
                     /codon_start=1
                     /product="vermillion"
                     /label=vermillion
                     /note="selectable marker"
                     /protein_id="ADE08348.1"
                     /translation="MSCPYAGNGNDHDDSAVPLTTEVGKIYGEYLMLDKLLDAQCMLSE
                     EDKRPVHDEHLFIITHQAYELWFKQIIFEFDSIRDMLDAEVIDETKTLEIVKRLNRVVL
                     ILKLLVDQVPILETMTPLDFMDFRKYLAPASGFQSLQFRLIENKLGVLTEQRVRYNQKY
                     SDVFSDEEARNSIRNSEKDPSLLELVQRWLERTPGLEESGFNFWAKFQESVDRFLEAQV
                     QSAMEEPVEKAKNYRLMDIEKRREVYRSIFDPAVHDALVRRGDRRFSHRALQGAIMITF
                     YRDEPRFSQPHQLLTLLMDIDSLITKWRYNHVIMVQRMIGSQQLGTGGSSGYQYLRSTL
                     SDRYKVFLDLFNLSTFLIPREAIPPLDETIRKKLINKSV"
     protein_bind    2640..2709
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     protein_bind    2947..3377
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     protein_bind    3395..3428
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3441..3535
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_recomb     3551..3584
                     /label=LoxP
                     /note="LoxP"
     protein_bind    complement(3551..3584)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    3591..3700
                     /label=5XUAS
                     /note="5XUAS"
     protein_bind    3597..3691
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3715..3953
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    3998..4122
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4147..4177
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             4231..4887
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             5232..5534
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(5578..5702)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          5763..5909
                     /note="ftz intron"
     intron          6009..6074
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6204..6224
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    6496..6630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6643..7028
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     promoter        complement(7088..7106)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7127..7143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7151..7167)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7175..7205)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7220..7241)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7529..8117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8291..9148)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9149..9240)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacaag
      661 cttatttatt ttgttatgtt atatgtatta tatgtcagac ataaagaaaa ggaacacatc
      721 aaatgtgata acaaagacta aacaagtaat tttattacac caaaacgaca aaacagtagg
      781 cagaacaaac aacgcatagc caaacattga cgaattggat accctgccga ttgtcagaca
      841 cttttgttga tcagtttctt gcgaatggtc tcgtccagcg gtggaatcgc ctcgcgggga
      901 atcagaaaag tggacagatt gaacagatcc agaaacacct tgtaccgatc actgaaacca
      961 aaaaaaaaca aagggagaac agtttgagtt cattgatccc cgatataatc acatctgcga
     1021 tgatcacctg agagtggagc gcagatattg atatccagac gagccaccag tgcccaactg
     1081 ttgggatcca atcatgcgtt gcaccatgat cacgtgattg tctgcggcgg gaatagaaag
     1141 tatttggtta ggaaaaccag tcttaaacat aagatatatt tataaaagag tatcaaagaa
     1201 tgcaatactt acatctccac ttggttatta acgagtcgat gtccatgagc agggtgagca
     1261 actggtgtgg ttggctgaac ctgggttcat ccctatagaa ggtgatcatg atggctccct
     1321 gaagggcacg atggctaaac cggcgatccc cacgacgcac cagtgcatcg tgcactgccg
     1381 gatcaaagat ggagcgatac acctcgcgtc gcttctcaat gtccatgagg cggtagtttt
     1441 tcgccttctc cacgggctcc tccatggcgc tctgtacctg cgcctccagg aatcgatcga
     1501 cgctctcctg aaacttggcc cagaagttga agccactctc ctccagtccg ggcgtcctct
     1561 ccagccatcg ctgcactagc tccagtagcg agggatcttt ctccgagttg cgaatcgagt
     1621 tccgcgcctc ctcgtcgcta aagacatccg agtacttctg gttgtatctc acccgctgct
     1681 ctgtcagaac tcccagcttg ttctcgatca aacggaactg cagcgactga aaaccagatg
     1741 cgggtgccag gtacttgcgg aagtccatga agtctagcgg ggtcatggtc tccagaatgg
     1801 gcacttggtc caccaggagc tgtacaaagg aagttataaa cggattttgg taagagattc
     1861 agaaagcact cacttttaga atcagaacca ctcggttcag tcgcttgaca atctccagcg
     1921 tcttggtttc atcgatgacc tctgcatcca acatgtctcg tatggagtcg aactcaaaga
     1981 tgatctgctt gaaccaaagc tcgtaggctg tggcgaaggt acttaaatgc cattgagtgt
     2041 tgtcatcaaa gttgtaaacc tactcaccct ggtgcgtgat gatgaacaga tgctcatcgt
     2101 gcacgggtcg cttgtcctcc tcggacagca tacactgggc atccagcagt ttgtccagca
     2161 tcagatactc tccatagatt ttgcccactt ccgtggttaa tggcaccgcc gaatcatcgt
     2221 gatcgtttct gtatgggttt gaattgaatc gcagaactga agatcgattg gcattcctgg
     2281 acagcacgtg ctggtgctca cccgtttcct gcatagggac agctcatggt gcacagctca
     2341 gatcagatcg tgactcctcg agcggcggat gctggcgaac tgatctccgc cagcggaccg
     2401 gagatgagac cccagcgaac cgataacaga gcgagagagc tccagttccg actgattgca
     2461 cagtcggtga tctgggcgat gggcactgcc agataggctg ggaattatca atcacttgag
     2521 gtgaaagtgc ggcgcacaca aataagcttg atatcatcga tctcgaggct gcatccaacg
     2581 cgttgggagc tctccggatc aattcggctt caggtaccgt cgacgatgta ggtcacggtc
     2641 tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc
     2701 tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac
     2761 gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc
     2821 acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg
     2881 acggtcacgg cgggcatgtc gacaagccga attgatccac tagaaggcct aattcggtac
     2941 actagttggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     3001 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     3061 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     3121 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     3181 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaaaaat
     3241 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     3301 ataccttttc ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg
     3361 gtgtgccaac caacaacact agtagtacca gcttataact tcgtataatg tatgctatac
     3421 gaagttatct gcaggcaggt cggagtactg tcctccgagc ggagtactgt cctccgagcg
     3481 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     3541 actcccatgg ataacttcgt ataatgtatg ctatacgaag ttatggatcc gcaggtcgga
     3601 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     3661 ctgtcctccg agcggagtac tgtcctccga gcggagactc gtcgacagcg agcgccggag
     3721 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
     3781 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc
     3841 agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg
     3901 caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaataggg
     3961 aattgggaat tccagatctg cggccgcggc tcgaatcaca agtttgtaca aaaaagctga
     4021 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
     4081 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc
     4141 ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg
     4201 gcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
     4261 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
     4321 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
     4381 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
     4441 ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
     4501 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
     4561 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
     4621 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
     4681 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
     4741 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
     4801 gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
     4861 tactgcgatg agtggcaggg cggggcgtaa acgcgtggat ccggcttact aaaagccaga
     4921 taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat
     4981 acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg acagttgaca
     5041 gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac
     5101 aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga
     5161 agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag
     5221 ggactggtga aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt
     5281 ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg
     5341 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg
     5401 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg
     5461 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
     5521 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat
     5581 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa
     5641 tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg
     5701 gtgattcgag ggtacctcta gagcaaacta gttctgatct gctagacaat tgttggcatc
     5761 aggtaggcat cacacacgat taacaacccc taaaaataca ctttgaaaat attgaaaata
     5821 tgtttttgta tacatttttg atattttcaa acaatacgca gttataaaac tcattagcta
     5881 acccattttt tctttgctta tgcttacaga ttgcaaagaa ctagagccgc gggatctttg
     5941 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     6001 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     6061 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     6121 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     6181 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     6241 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     6301 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     6361 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
     6421 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     6481 ggaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6541 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6601 ctcatcaatg tatcttatca tgtctggttc cagagctctg gccacgtaat aagtgtgcgt
     6661 tgaatttatt cgcaaaaaca ttgcatattt tcggcaaagt aaaattttgt tgcatacctt
     6721 atcaaaaaat aagtgctgca tactttttag agaaaccaaa taatttttta ttgcataccc
     6781 gtttttaata aaatacattg cataccctct tttaataaaa aatattgcat actttgacga
     6841 aacaaatttt cgttgcatac ccaataaaag attattatat tgcatacccg tttttaataa
     6901 aatacattgc ataccctctt ttaataaaaa atattgcata cgttgacgaa acaaattttc
     6961 gttgcatacc caataaaaga ttattatatt gcataccttt tcttgccata ccatttagcc
     7021 gatcaattgt gctcggcaac agtatatttg tggtgtgcca accaacaacg agctccagct
     7081 tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc
     7141 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7201 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7261 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7321 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7381 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7441 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7501 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7561 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7621 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7681 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7741 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7801 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7861 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7921 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7981 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8041 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8101 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8161 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8221 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8281 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8341 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8401 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8461 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8521 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8581 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8641 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8701 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8761 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8821 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8881 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8941 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9001 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9061 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9121 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9181 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
//
