LOCUS       40924_45878        7541 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Overexpression vector pVALIUM10-moe, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Ni J-Q., Liu L-P., Perkins LA, Perrimon N.
  TITLE     Vectors for transgenic flies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Ni J-Q., Liu L-P., Perkins LA, Perrimon N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2010) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 7541)
  AUTHORS   Hu Y, Perkins LA.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2012) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   4  (bases 1 to 7541)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7541)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-FEB-2010) Genetics, Harvard Medical School, 77 Avenue Louis 
            Pasteur, Boston, MA 02115, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (20-JUN-2012) Genetics, Harvard Medical School, 77 Avenue Louis 
            Pasteur, Boston, MA 02115, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Jun 20, 2012 this sequence version replaced GU931387.1.
FEATURES             Location/Qualifiers
     source          1..7541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    664..2543
                     /label=vermilion
                     /note="vermilion"
     CDS             complement(join(834..951,1028..1121,1213..1819,1874..2007,
                     2068..2228,2302..2327))
                     /codon_start=1
                     /product="vermillion"
                     /label=vermillion
                     /note="selectable marker"
                     /protein_id="ADE08349.1"
                     /translation="MSCPYAGNGNDHDDSAVPLTTEVGKIYGEYLMLDKLLDAQCMLSE
                     EDKRPVHDEHLFIITHQAYELWFKQIIFEFDSIRDMLDAEVIDETKTLEIVKRLNRVVL
                     ILKLLVDQVPILETMTPLDFMDFRKYLAPASGFQSLQFRLIENKLGVLTEQRVRYNQKY
                     SDVFSDEEARNSIRNSEKDPSLLELVQRWLERTPGLEESGFNFWAKFQESVDRFLEAQV
                     QSAMEEPVEKAKNYRLMDIEKRREVYRSIFDPAVHDALVRRGDRRFSHRALQGAIMITF
                     YRDEPRFSQPHQLLTLLMDIDSLITKWRYNHVIMVQRMIGSQQLGTGGSSGYQYLRSTL
                     SDRYKVFLDLFNLSTFLIPREAIPPLDETIRKKLINKSV"
     protein_bind    2640..2709
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     protein_bind    2947..3377
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     protein_bind    3395..3428
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3441..3535
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_recomb     3551..3584
                     /label=LoxP
                     /note="LoxP"
     protein_bind    complement(3551..3584)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    3591..3700
                     /label=5XUAS
                     /note="5XUAS"
     protein_bind    3597..3691
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3715..3953
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    3967..3990
                     /label=multiple cloning site
                     /note="multiple cloning site"
     intron          4031..4177
                     /note="ftz intron"
     intron          4277..4342
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4472..4492
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4764..4898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    4907..5337
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    4907..5337
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     promoter        complement(5356..5374)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5395..5411)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5419..5435)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5443..5473)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5488..5509)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5797..6385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6559..7416)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7417..7521)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacaag
      661 cttatttatt ttgttatgtt atatgtatta tatgtcagac ataaagaaaa ggaacacatc
      721 aaatgtgata acaaagacta aacaagtaat tttattacac caaaacgaca aaacagtagg
      781 cagaacaaac aacgcatagc caaacattga cgaattggat accctgccga ttgtcagaca
      841 cttttgttga tcagtttctt gcgaatggtc tcgtccagcg gtggaatcgc ctcgcgggga
      901 atcagaaaag tggacagatt gaacagatcc agaaacacct tgtaccgatc actgaaacca
      961 aaaaaaaaca aagggagaac agtttgagtt cattgatccc cgatataatc acatctgcga
     1021 tgatcacctg agagtggagc gcagatattg atatccagac gagccaccag tgcccaactg
     1081 ttgggatcca atcatgcgtt gcaccatgat cacgtgattg tctgcggcgg gaatagaaag
     1141 tatttggtta ggaaaaccag tcttaaacat aagatatatt tataaaagag tatcaaagaa
     1201 tgcaatactt acatctccac ttggttatta acgagtcgat gtccatgagc agggtgagca
     1261 actggtgtgg ttggctgaac ctgggttcat ccctatagaa ggtgatcatg atggctccct
     1321 gaagggcacg atggctaaac cggcgatccc cacgacgcac cagtgcatcg tgcactgccg
     1381 gatcaaagat ggagcgatac acctcgcgtc gcttctcaat gtccatgagg cggtagtttt
     1441 tcgccttctc cacgggctcc tccatggcgc tctgtacctg cgcctccagg aatcgatcga
     1501 cgctctcctg aaacttggcc cagaagttga agccactctc ctccagtccg ggcgtcctct
     1561 ccagccatcg ctgcactagc tccagtagcg agggatcttt ctccgagttg cgaatcgagt
     1621 tccgcgcctc ctcgtcgcta aagacatccg agtacttctg gttgtatctc acccgctgct
     1681 ctgtcagaac tcccagcttg ttctcgatca aacggaactg cagcgactga aaaccagatg
     1741 cgggtgccag gtacttgcgg aagtccatga agtctagcgg ggtcatggtc tccagaatgg
     1801 gcacttggtc caccaggagc tgtacaaagg aagttataaa cggattttgg taagagattc
     1861 agaaagcact cacttttaga atcagaacca ctcggttcag tcgcttgaca atctccagcg
     1921 tcttggtttc atcgatgacc tctgcatcca acatgtctcg tatggagtcg aactcaaaga
     1981 tgatctgctt gaaccaaagc tcgtaggctg tggcgaaggt acttaaatgc cattgagtgt
     2041 tgtcatcaaa gttgtaaacc tactcaccct ggtgcgtgat gatgaacaga tgctcatcgt
     2101 gcacgggtcg cttgtcctcc tcggacagca tacactgggc atccagcagt ttgtccagca
     2161 tcagatactc tccatagatt ttgcccactt ccgtggttaa tggcaccgcc gaatcatcgt
     2221 gatcgtttct gtatgggttt gaattgaatc gcagaactga agatcgattg gcattcctgg
     2281 acagcacgtg ctggtgctca cccgtttcct gcatagggac agctcatggt gcacagctca
     2341 gatcagatcg tgactcctcg agcggcggat gctggcgaac tgatctccgc cagcggaccg
     2401 gagatgagac cccagcgaac cgataacaga gcgagagagc tccagttccg actgattgca
     2461 cagtcggtga tctgggcgat gggcactgcc agataggctg ggaattatca atcacttgag
     2521 gtgaaagtgc ggcgcacaca aataagcttg atatcatcga tctcgaggct gcatccaacg
     2581 cgttgggagc tctccggatc aattcggctt caggtaccgt cgacgatgta ggtcacggtc
     2641 tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc
     2701 tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac
     2761 gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc
     2821 acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg
     2881 acggtcacgg cgggcatgtc gacaagccga attgatccac tagaaggcct aattcggtac
     2941 actagttggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     3001 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     3061 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     3121 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     3181 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaaaaat
     3241 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     3301 ataccttttc ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg
     3361 gtgtgccaac caacaacact agtagtacca gcttataact tcgtataatg tatgctatac
     3421 gaagttatct gcaggcaggt cggagtactg tcctccgagc ggagtactgt cctccgagcg
     3481 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     3541 actcccatgg ataacttcgt ataatgtatg ctatacgaag ttatggatcc gcaggtcgga
     3601 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     3661 ctgtcctccg agcggagtac tgtcctccga gcggagactc gtcgacagcg agcgccggag
     3721 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
     3781 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc
     3841 agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg
     3901 caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaataggg
     3961 aattgggaat tccagatctg acagtctaga gcaaactagt tctgatctgc tagacaattg
     4021 ttggcatcag gtaggcatca cacacgatta acaaccccta aaaatacact ttgaaaatat
     4081 tgaaaatatg tttttgtata catttttgat attttcaaac aatacgcagt tataaaactc
     4141 attagctaac ccattttttc tttgcttatg cttacagatt gcaaagaact agagccgcgg
     4201 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
     4261 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
     4321 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
     4381 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
     4441 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
     4501 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
     4561 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
     4621 tatggaaaaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     4681 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     4741 aaatgaatgg aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     4801 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4861 tgtccaaact catcaatgta tcttatcatg tctggttcca gagctctggc cacgtaataa
     4921 gtgtgcgttg aatttattcg caaaaacatt gcatattttc ggcaaagtaa aattttgttg
     4981 cataccttat caaaaaataa gtgctgcata ctttttagag aaaccaaata attttttatt
     5041 gcatacccgt ttttaataaa atacattgca taccctcttt taataaaaaa tattgcatac
     5101 tttgacgaaa caaattttcg ttgcataccc aataaaagat tattatattg catacccgtt
     5161 tttaataaaa tacattgcat accctctttt aataaaaaat attgcatacg ttgacgaaac
     5221 aaattttcgt tgcataccca ataaaagatt attatattgc ataccttttc ttgccatacc
     5281 atttagccga tcaattgtgc tcggcaacag tatatttgtg gtgtgccaac caacaacgag
     5341 ctccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
     5401 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5461 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5521 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5581 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5641 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5701 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5761 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     5821 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     5881 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5941 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6001 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6061 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6121 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6181 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     6241 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6301 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6361 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6421 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6481 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6541 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6601 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6661 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6721 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6781 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6841 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     6901 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6961 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7021 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7081 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7141 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7201 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7261 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7321 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7381 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7441 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7501 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg c
//
