LOCUS       V002258                 9065 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002258
VERSION     V002258
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Honeyman AL, Cote CK, Curtiss R III.
  TITLE     Construction of transcriptional and translational lacZ gene reporter
            plasmids for use in Streptococcus mutans
  JOURNAL   J. Microbiol. Methods 49 (2), 163-171 (2002)
   PUBMED   11830302
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   Honeyman AL, Cote CK, Curtiss R III.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2001) Department of Medical Microbiology and
            Immunology, University of South Florida, 12901 Bruce B. Downs Blvd.,
            Tampa, FL 33612, USA
REFERENCE   3  (bases 1 to 9065)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9065)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Microbiol. Methods"; date: "2002"; volume: "49"; issue: "2"; pages:
            "163-171"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-AUG-2001) Department of Medical Microbiology and Immunology,
            University of South Florida, 12901 Bruce B. Downs Blvd., Tampa, FL
            33612, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             58..1245
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             complement(2285..2941)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2942..3044)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(3570..4115)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             6077..6790
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label="rep"
                     /note="required for replication in Gram positive hosts"
                     /protein_id="AAN02503.1"
                     /translation="MKYAYQAELVVNEAMKRYPKGRFLFLTLTIKNISGEKLNKSISEI
                     GRAFNRLMKYKKVDKNVIGYLRATEVTYSTEHENYHPHLHVLLFVKSSYFTGNNTNYIS
                     QEEWTKLWAKAMKLDYTPVVDIRTVKAHKRKNLKSAIIETAKYPVKPFDVDTEDVTLFS
                     EMVKERITEDLTNGLHRKRQIGFGKLFKKIKAELALDDVEEGNLVQTGAEESAESTGRE
                     IVAFWNWDRKNYFVR"
     gene            6077..6790
                     /gene="rep"
                     /label="rep"
     CDS             complement(7418..8152)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
ORIGIN
        1 aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg
       61 aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata
      121 ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc cgacagcatc
      181 gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct atgcgcaccc
      241 gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc ttcgctactt
      301 ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat cctctacgcc
      361 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
      421 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
      481 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
      541 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
      601 caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa cccagtcagc
      661 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
      721 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
      781 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
      841 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
      901 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
      961 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     1021 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     1081 gctcttacca gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc
     1141 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
     1201 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc
     1261 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa
     1321 ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca
     1381 gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc
     1441 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat
     1501 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa
     1561 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtccccta
     1621 cgtgctgctg aagttgcccg caacagagag tggaaccaac cggtgatacc acgatactat
     1681 gactgagagt caacgccatg agcggcctca tttcttattc tgagttacaa cagtccgcac
     1741 cgctgtccgg tagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     1801 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgcccaac agtcccccgg
     1861 ccacggggcc tgccaccata cccacgccga aacaagcgcc ctgcaccatt atgttccgga
     1921 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc
     1981 gctaaccgtt tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac
     2041 ctccacgggg agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact
     2101 gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc
     2161 ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta
     2221 ttatcactta ttcaggcgta gcaccaggcg tttaagggca ccaataactg ccttaaaaaa
     2281 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2341 catggaagcc atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2401 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2461 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2521 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2581 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2641 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2701 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     2761 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2821 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2881 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2941 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     3001 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc
     3061 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
     3121 agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact
     3181 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
     3241 tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat
     3301 cagcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag
     3361 tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag
     3421 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
     3481 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
     3541 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
     3601 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
     3661 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     3721 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
     3781 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
     3841 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc
     3901 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
     3961 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
     4021 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag
     4081 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
     4141 aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
     4201 tacgatataa gttgtaattc tcatgtttga cagcttatca tcgataagct ttttggcatg
     4261 aaaaagagtt tgctgatatg tttgaagttc aatctccgat acgtgaaaca actaaccgag
     4321 aaaaaatgga ctggttaaga aaacagtacc aagaagagct gaaaaaacta gaatcgtcta
     4381 aaaagcccct agaagacgat ttaagccatt tagaagagtt gcttgatgaa aagaccaagg
     4441 aatatattaa aatcgattct gagacctctg agaggctctc agagctatct aaagctgagg
     4501 gatatataaa taccttagaa aattattcga agagcttaga agcgaaaata gagcgtttag
     4561 agcgcgaggg gctgaaatta gaaaaactaa aaacacaaat agctgaccta aaaatcatgt
     4621 ctgagaaaga actagcggct attaccccta aaaaaggcgt gttcggtaaa gaatatgtgg
     4681 aattgactaa agagcagttt gaagaattta aagggctgat ataccgaagc agaaaccttg
     4741 ttcatcaaaa agagctagag aatgagcaat taaggcggat agtgcctctg agacgctcta
     4801 aacggtttga agcgagttgg aacgagctaa agaaaaaagt aagggagaga gcatagagcg
     4861 tcttaggaac gaaaatagag cgcttagaag tgaaaactca gttttgagac agcaaaatga
     4921 caaaatgcta ggaaaactga aagagtttat gccagataaa gccttaaaaa attttatatc
     4981 agagttaaga gctattcagc caatcgtaag ggtagttaaa cgagtgattg aaaaagggct
     5041 aggcctttga gcgatttatg ccgtgaaagc taattgacaa taagcaaggg caaagtacgc
     5101 taggacgtga cgagccgaaa ggctttagcg tttcgagccg acacggacaa aggacgtccg
     5161 cccttggtta cttgttgtca attagaccat ggaataaagt aagcggacat ggtataatag
     5221 ctaggtcgca acgttctttc gctaagttac gaacttagat tggaggtgag cgctgtgaag
     5281 actttcctag aacttgtttt gatacctttt gtggttggcg ttgctgcaga ggtaagtgct
     5341 gattggttga ttcggtatgt tcgaaacaaa cgcgacaaaa attaacctga gttctttttg
     5401 aggacaaaaa agaaagacag tagttccagc tactgttttt tttgcgttgt gctattcgtt
     5461 tcctagaact tctagcgtta aaattattat accacgtttg gatttagaaa gtcaaaattt
     5521 gaggttttag gggtgaattt ttcgtgaaac gaaaaagagg gctgaaaagc ccttaaaaac
     5581 ccaattgcgt agcaagggtt ttttcttatc ttgatacata tagaaataat gagttttttt
     5641 attttcttgt tttaaagcac ctcaaaccct tgatattgct gggtttttag gtaacaaaaa
     5701 agcccttgca atttaataaa ataaagttta taatttaagt gtccaattta aaataataaa
     5761 cttaggagaa ttgcaggaac ttttttatat actcaaaaaa atttttttgc aagaaaatta
     5821 taacatgaca ggtactgaaa atcaagtctt taaggactat tctaagaatg gcaaagatag
     5881 aaagtggcga gaacgcaagt taaaaaatat tgagcttgct ggtcgtttag agcgtttagg
     5941 atatcgttcg tttgaacggg tctatcaatg tgccgaagtg ttgaagttta tcgaacaaca
     6001 agacggcacg aaaaaactct atcagtctta tttttgcaaa aataagctct gcccaatttg
     6061 taactggaga cggtcaatga agtatgctta tcaagctgaa ttagtggtaa atgaagcaat
     6121 gaaacgctat ccaaaaggtc gctttctctt tttgaccttg acgattaaga acattagtgg
     6181 tgaaaaatta aataaatcaa tttcagaaat agggcgagct tttaatcgtc ttatgaaata
     6241 caagaaagtc gataaaaatg ttattggcta tttgcgagcc actgaggtaa cttattcaac
     6301 tgagcatgag aattatcacc ctcatttgca tgtattgtta tttgtgaaat ctagctattt
     6361 tactggaaat aatacaaatt acattagcca agaagaatgg acgaaactat gggctaaggc
     6421 gatgaaattg gattatacac ctgtagttga tattcgaacc gtcaaagctc ataaacgtaa
     6481 aaacttgaag tcagctatta tcgaaacagc taaatatcct gttaagcctt ttgatgtaga
     6541 tacagaagat gtgacattat tctctgaaat ggtcaaagaa cggataacag aagatttaac
     6601 gaatggtttg caccgaaaaa ggcagattgg ttttggaaag ttgttcaaga aaatcaaggc
     6661 ggagttagct cttgatgatg tcgaagaagg gaatcttgtt cagaccggag cagaagaatc
     6721 tgcagaaagt actggtcgtg aaattgttgc cttttggaat tgggatagaa agaattattt
     6781 tgtgaggtag ctgatgagta aaacagtatc agaattagct caagaattgg gagttagtag
     6841 gcagtatctt aatcggattt tatcgcaaaa taatctcggt cgaaaaaaag ggaataaaaa
     6901 agtagtttcc gatatggacg agaaagtact catatcaata cttgagggca atattggaaa
     6961 ccaaaaaacg gaaactaaaa cggaaacaaa aacattagtt tccaatatgt ctttaagctc
     7021 aagtgagata aggaattgtg ctaaactacc agttaagatg ttgctgatta agacgagcaa
     7081 tagactgtgt cggctcgtct gtctttcggt aatcgaaaga cagtttttgg ggagggaaat
     7141 ctatgagtta catggttgca agaatgcaga agatgaaagc tggcaatttg ggaggagctt
     7201 ttaagcataa tgaacgtgtt tttgagacgc actcaaataa agatatagac ccgagcgctt
     7261 agtgggaatt tgtacccctt atcgatacaa attccccgta ggcgctaggg acctctttag
     7321 ctccttggaa gctgtcagta gtatacctaa taatttatct acattccctt tagtaacgtg
     7381 taactttcca aatttacaaa agcgactcat agaattattt cctcccgtta aataatagat
     7441 aactattaaa aatagacaat acttgctcat aagtaacggt acttaaattg tttactttgg
     7501 cgtgtttcat tgcttgatga aactgatttt tagtaaacag ttgacgatat tctcgattga
     7561 cccattttga aacaaagtac gtatatagct tccaatattt atctggaaca tctgtggtat
     7621 ggcgggtaag ttttattaag acactgttta cttttggttt aggatgaaag cattccgctg
     7681 gcagcttaag caattgctga atcgagactt gagtgtgcaa gagcaaccct agtgttcggt
     7741 gaatatccaa ggtacgcttg tagaatcctt cttcaacaat cagatagatg tcagacgcat
     7801 ggctttcaaa aaccactttt ttaataattt gtgtgcttaa atggtaagga atactcccaa
     7861 caattttata cctctgtttg ttagggaatt gaaactgtag aatatcttgg tgaattaaag
     7921 tgacacgagt attcagtttt aatttttctg acgataagtt gaatagatga ctgtctaatt
     7981 caatagacgt tacctgttta cttattttag ccagtttcgt cgttaaatgc cctttacctg
     8041 ttccaatttc gtaaacggta tcggtttctt ttaaattcaa ttgttttatt atttggttga
     8101 gtactttttc actcgttaaa aagttttgag aatattttat atttttgttc atgtaatcac
     8161 tccttcttaa ttacaaattt ttagcatcta atttaacttc aattcctatt atacaaaatt
     8221 ttaagatact gcactatcaa cacactctta agtttgcttc taagtcttat ttccataact
     8281 tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg caagtcagca
     8341 cgaacacgaa ccgtcttatc tcccattata tctttttttg cactgattgg tgtatcattt
     8401 cgtttttctt ttgtgcgcct aaatttccca caatcactca cttctttcta tttcttctta
     8461 ttcttatttt atcatcaaca atcacaaatc acttgtgatt tgtgataagt gatttgtgat
     8521 taatatataa aagccctctt taaagggctt ttatgtttat tttgagaaag atataaaatc
     8581 aatatatccc ttttctccga ttttacaacg gcattgtagg actttctatc tttcgttttg
     8641 attcctttta ccagggtttc ttttccctct agtaattctt ttacatttgt tttggtgagt
     8701 tttttctttc taaaatgttc agctaaagta aacttacatt caggataatt tgaacaacca
     8761 taaaacgatt tttttaatac aatattgttg ccacacttag gacattttcc tacaatactt
     8821 ttttctgctt ctttttcttt ctgttcctgg taatcagaaa aatttagttt ttctatatcg
     8881 ttaggtacag gactccagta aatgaacaat gaatttttga tattcgtaat aaagttctct
     8941 tgattgcctt ctcttttacc gatttttttt aaatacgttt cccatttagc cgtcatttca
     9001 gcactcgtta aaaggtgctg actttcaact gcctggcaca ataattttcc tttttcagtt
     9061 acaac
//
