LOCUS       40924_45853        7551 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pV3lacZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Hertel R, Volland S, Liesegang H.
  TITLE     Conjugative reporter system for the use in Bacillus licheniformis 
            and closely related Bacilli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7551)
  AUTHORS   Hertel R, Volland S, Liesegang H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2014) Genomic and Applied Microbiology, Goettingen
            Genomics Laboratory, Georg-August University Goettingen, 
            Grisebachstr.8, Goettingen, Lower Saxony D-37077, Germany
REFERENCE   3  (bases 1 to 7551)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7551)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAR-2014) Genomic and Applied Microbiology, Goettingen Genomics 
            Laboratory, Georg-August University Goettingen, Grisebachstr.8, 
            Goettingen, Lower Saxony D-37077, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..119
                     /label=AmpR promoter
     CDS             120..977
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1151..1739
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2023..5067
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     CDS             complement(5132..5902)
                     /codon_start=1
                     /gene="kan"
                     /product="kanamycin nucleotidyltransferase"
                     /label=kan
                     /protein_id="AHZ90964.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGR
                     QTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHG
                     QFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTT
                     FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSD
                     SEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     gene            complement(5132..5902)
                     /gene="kan"
                     /label=kan
     oriT            6194..6303
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(6334..7335)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     rep_origin      complement(7487..7513)
                     /direction=LEFT
                     /label=oriU
                     /note="oriU"
ORIGIN
        1 gatccggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
       61 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      121 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      181 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      241 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      301 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      361 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      421 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      481 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      541 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      601 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      661 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      721 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      781 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
      841 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
      901 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
      961 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1021 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1081 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1141 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1201 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1261 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1321 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1381 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1441 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1501 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1561 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1621 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1681 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1741 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1801 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     1861 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     1921 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagacgcg
     1981 cctcgagcgg ccgccttaag gtaccgaatt cccggggatc ccgtcgtttt acaacgtcgt
     2041 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     2101 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     2161 aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg
     2221 gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt
     2281 tacgatgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt
     2341 cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta
     2401 caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc
     2461 aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc
     2521 gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc
     2581 agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg
     2641 ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat
     2701 ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta
     2761 cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc
     2821 ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac
     2881 gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa
     2941 ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag
     3001 gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt
     3061 aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag
     3121 gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac
     3181 catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat
     3241 attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg
     3301 gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc
     3361 atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg
     3421 atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg
     3481 gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct
     3541 gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc
     3601 ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag
     3661 gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg
     3721 ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat
     3781 acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat
     3841 ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa
     3901 accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg
     3961 atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca
     4021 caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc
     4081 tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc
     4141 agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc
     4201 cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag
     4261 cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa
     4321 caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc
     4381 gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc
     4441 cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc tgatgcggtg
     4501 ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa
     4561 acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtggcgagc
     4621 gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg
     4681 gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt
     4741 tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa
     4801 aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac
     4861 ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat
     4921 ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt
     4981 ggcgacgact cctggagccc gtcagtatcg gcggaattac agctgagcgc cggtcgctac
     5041 cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg tctgcccgta
     5101 tttcgcgtaa ggaaatccgg cgcgccatcg ttcaaaatgg tatgcgtttt gacacatcca
     5161 ctatatatcc gtgtcgttct gtccactcct gaatcccatt ccagaaattc tctagcgatt
     5221 ccagaagttt ctcagagtcg gaaagttgac cagacattac gaactggcac agatggtcat
     5281 aacctgaagg aagatctgat tgcttaactg cttcagttaa gaccgaagcg ctcgtcgtat
     5341 aacagatgcg atgatgcaga ccaatcaaca tggcacctgc cattgctacc tgtacagtca
     5401 aggatggtag aaatgttgtc ggtccttgca cacgaatatt acgccatttg cctgcatatt
     5461 caaacagctc ttctacgata agggcacaaa tcgcatcgtg gaacgtttgg gcttctaccg
     5521 atttagcagt ttgatacact ttctctaagt atccacctga atcataaatc ggcaaaatag
     5581 agaaaaattg accatgtgta agcggccaat ctgattccac ctgagatgca taatctagta
     5641 gaatctcttc gctatcaaaa ttcacttcca ccttccactc accggttgtc cattcatggc
     5701 tgaactctgc ttcctctgtt gacatgacac acatcatctc aatatccgaa tagggcccat
     5761 cagtctgacg accaagagag ccataaacac caatagcctt aacatcatcc ccatatttat
     5821 ccaatattcg ttccttaatt tcatgaacaa tcttcattct ttcttctcta gtcattatta
     5881 ttggtccatt cactattctc attccctttt cagataattt tagatttgct tttctaaata
     5941 agaatatttg gagagcaccg ttcttattca gctattaata actcgtcttc ctaagcatcc
     6001 ttcaatcctt ttaataacaa ttatagcatc taatcttcaa caaactggcc cgtttgttga
     6061 acgcgcgcgt acgactagtc ttcggggtca ttatagcgat tttttcggta tatccatcct
     6121 ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc
     6181 aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct
     6241 tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc
     6301 cggctaccgc cggcgtaaca gatgtgatca ctactcttta ataaaataat ttttccgttc
     6361 ccaattccac attgcaataa tagaaaatcc atcttcatcg gctttttcgt catcatctgt
     6421 atgaatcaaa tcgccttctt ctgtgtcatc aaggtttaat tttttatgta tttcttttaa
     6481 caaaccacca taggagatta accttttacg gtgtaaacct tcctccaaat cagacaaacg
     6541 tttcaaattc ttttcttcat catcggtcat aaaatccgta tcctttacag gatattttgc
     6601 agtttcgtca attgccgatt gtatatccga tttatattta tttttcggtc gaatcatttg
     6661 aacttttaca tttggatcat agtctaattt cattgccttt ttccaaaatt gaatccattg
     6721 tttttgattc acgtagtttt ctgtattctt aaaataagtt ggttccacac ataccaatac
     6781 atgcatgtgc tgattataag aattatcttt attatttatt gtcacttccg ttgcacgcat
     6841 aaaaccaaca agatttttat taattttttt atattgcatc attcggcgaa atccttgagc
     6901 catatctgac aaactcttat ttaattcttc gccatcataa acatttttaa ctgttaatgt
     6961 gagaaacaac caacgaactg ttggcttttg tttaataact tcagcaacaa ccttttgtga
     7021 ctgaatgcca tgtttcattg ctctcctcca gttgcacatt ggacaaagcc tggatttaca
     7081 aaaccacact cgatacaact ttctttcgcc tgtttcacga ttttgtttat actctaatat
     7141 ttcagcacaa tcttttactc tttcagcctt tttaaattca agaatatgca gaagttcaaa
     7201 gtaatcaaca ttagcgattt tcttttctct ccatggtctc acttttccac tttttgtctt
     7261 gtccactaaa acccttgatt tttcatctga ataaatgcta ctattaggac acataatatt
     7321 aaaagaaacc cccatctatt tagttatttg tttagtcact tataacttta acagatgggg
     7381 tttttctgtg caaccaattt taagggtttt caatacttta aaacacatac ataccaacac
     7441 ttcaacgcac ctttcagcaa ctaaaataaa aatgacgtta tttctatatg tatcaagata
     7501 agaaagaaca agttcaaaac catcaaaaaa agacaccttt tcaggtgctt g
//
