LOCUS       40924_45858        7586 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pV3SDlacZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Hertel R, Volland S, Liesegang H.
  TITLE     Conjugative reporter system for the use in Bacillus licheniformis 
            and closely related Bacilli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7586)
  AUTHORS   Hertel R, Volland S, Liesegang H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2014) Genomic and Applied Microbiology, Goettingen
            Genomics Laboratory, Georg-August University Goettingen, 
            Grisebachstr.8, Goettingen, Lower Saxony D-37077, Germany
REFERENCE   3  (bases 1 to 7586)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7586)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAR-2014) Genomic and Applied Microbiology, Goettingen Genomics 
            Laboratory, Georg-August University Goettingen, Grisebachstr.8, 
            Goettingen, Lower Saxony D-37077, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..119
                     /label=AmpR promoter
     CDS             120..977
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1151..1739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2076..5102
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="DRENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWR
                     FAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTEN
                     PTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFL
                     RAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFS
                     RAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNV
                     ENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGV
                     NRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEA
                     NIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALY
                     RWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETR
                     PLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGG
                     DFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDN
                     ELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAW
                     SEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ
                     MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCT
                     ADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLN
                     CQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTREL
                     NYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSV
                     SAELQLSAGRYHYQLVWCQK"
     CDS             complement(5167..5937)
                     /codon_start=1
                     /gene="kan"
                     /product="kanamycin nucleotidyltransferase"
                     /label=kan
                     /protein_id="AHZ90958.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGR
                     QTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHG
                     QFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTT
                     FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSD
                     SEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     gene            complement(5167..5937)
                     /gene="kan"
                     /label=kan
     oriT            6229..6338
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(6369..7370)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     rep_origin      complement(7522..7548)
                     /direction=LEFT
                     /label=oriU
                     /note="oriU"
ORIGIN
        1 gatccggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
       61 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      121 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      181 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      241 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      301 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      361 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      421 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      481 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      541 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      601 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      661 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      721 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      781 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
      841 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
      901 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
      961 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1021 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1081 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1141 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1201 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1261 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1321 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1381 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1441 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1501 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1561 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1621 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1681 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1741 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1801 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     1861 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     1921 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagacgcg
     1981 cctcgagcgg ccgccttaag gtaccgaatt cccggggatc cacagtacat aaaaaaggag
     2041 acatgacgat ggtcgtttta caacgtgact gggtcgaccg ggaaaaccct ggcgttaccc
     2101 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     2161 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt
     2221 ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata
     2281 ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg
     2341 tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt
     2401 actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt
     2461 ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc
     2521 aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc
     2581 gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat caggatatgt
     2641 ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca
     2701 gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta ctggaggctg
     2761 aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct ttatggcagg
     2821 gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg
     2881 gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg
     2941 ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga
     3001 ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc
     3061 tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc
     3121 atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca
     3181 actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg
     3241 accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa
     3301 tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa
     3361 tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag
     3421 gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc
     3481 cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt tgcccgatgt
     3541 acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat
     3601 ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg
     3661 gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac
     3721 agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca
     3781 acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta
     3841 tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc
     3901 agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc
     3961 tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc
     4021 tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc
     4081 ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc gtagtgcaac
     4141 cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg
     4201 cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca
     4261 gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag
     4321 gctttctttc acagatgtgg attggcgata aaaaacaact gctgacgccg ctgcgcgatc
     4381 agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc cgcattgacc
     4441 ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt
     4501 tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct cacgcgtggc
     4561 agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc
     4621 aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg
     4681 gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga ttagggccgc
     4741 aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt
     4801 cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg
     4861 aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca
     4921 gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat
     4981 ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag
     5041 tatcggcgga attacagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa
     5101 aataataata accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccggcgcgc
     5161 catcgttcaa aatggtatgc gttttgacac atccactata tatccgtgtc gttctgtcca
     5221 ctcctgaatc ccattccaga aattctctag cgattccaga agtttctcag agtcggaaag
     5281 ttgaccagac attacgaact ggcacagatg gtcataacct gaaggaagat ctgattgctt
     5341 aactgcttca gttaagaccg aagcgctcgt cgtataacag atgcgatgat gcagaccaat
     5401 caacatggca cctgccattg ctacctgtac agtcaaggat ggtagaaatg ttgtcggtcc
     5461 ttgcacacga atattacgcc atttgcctgc atattcaaac agctcttcta cgataagggc
     5521 acaaatcgca tcgtggaacg tttgggcttc taccgattta gcagtttgat acactttctc
     5581 taagtatcca cctgaatcat aaatcggcaa aatagagaaa aattgaccat gtgtaagcgg
     5641 ccaatctgat tccacctgag atgcataatc tagtagaatc tcttcgctat caaaattcac
     5701 ttccaccttc cactcaccgg ttgtccattc atggctgaac tctgcttcct ctgttgacat
     5761 gacacacatc atctcaatat ccgaataggg cccatcagtc tgacgaccaa gagagccata
     5821 aacaccaata gccttaacat catccccata tttatccaat attcgttcct taatttcatg
     5881 aacaatcttc attctttctt ctctagtcat tattattggt ccattcacta ttctcattcc
     5941 cttttcagat aattttagat ttgcttttct aaataagaat atttggagag caccgttctt
     6001 attcagctat taataactcg tcttcctaag catccttcaa tccttttaat aacaattata
     6061 gcatctaatc ttcaacaaac tggcccgttt gttgaacgcg cgcgtacgac tagtcttcgg
     6121 ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg
     6181 ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg
     6241 tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc
     6301 ggtgctcaac gggaatcctg ctctgcgagg ctggccggct accgccggcg taacagatgt
     6361 gatcactact ctttaataaa ataatttttc cgttcccaat tccacattgc aataatagaa
     6421 aatccatctt catcggcttt ttcgtcatca tctgtatgaa tcaaatcgcc ttcttctgtg
     6481 tcatcaaggt ttaatttttt atgtatttct tttaacaaac caccatagga gattaacctt
     6541 ttacggtgta aaccttcctc caaatcagac aaacgtttca aattcttttc ttcatcatcg
     6601 gtcataaaat ccgtatcctt tacaggatat tttgcagttt cgtcaattgc cgattgtata
     6661 tccgatttat atttattttt cggtcgaatc atttgaactt ttacatttgg atcatagtct
     6721 aatttcattg cctttttcca aaattgaatc cattgttttt gattcacgta gttttctgta
     6781 ttcttaaaat aagttggttc cacacatacc aatacatgca tgtgctgatt ataagaatta
     6841 tctttattat ttattgtcac ttccgttgca cgcataaaac caacaagatt tttattaatt
     6901 tttttatatt gcatcattcg gcgaaatcct tgagccatat ctgacaaact cttatttaat
     6961 tcttcgccat cataaacatt tttaactgtt aatgtgagaa acaaccaacg aactgttggc
     7021 ttttgtttaa taacttcagc aacaaccttt tgtgactgaa tgccatgttt cattgctctc
     7081 ctccagttgc acattggaca aagcctggat ttacaaaacc acactcgata caactttctt
     7141 tcgcctgttt cacgattttg tttatactct aatatttcag cacaatcttt tactctttca
     7201 gcctttttaa attcaagaat atgcagaagt tcaaagtaat caacattagc gattttcttt
     7261 tctctccatg gtctcacttt tccacttttt gtcttgtcca ctaaaaccct tgatttttca
     7321 tctgaataaa tgctactatt aggacacata atattaaaag aaacccccat ctatttagtt
     7381 atttgtttag tcacttataa ctttaacaga tggggttttt ctgtgcaacc aattttaagg
     7441 gttttcaata ctttaaaaca catacatacc aacacttcaa cgcacctttc agcaactaaa
     7501 ataaaaatga cgttatttct atatgtatca agataagaaa gaacaagttc aaaaccatca
     7561 aaaaaagaca ccttttcagg tgcttg
//
