LOCUS       40924_45848        4423 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pV2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Hertel R, Volland S, Liesegang H.
  TITLE     Conjugative reporter system for the use in Bacillus licheniformis 
            and closely related Bacilli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Hertel R, Volland S, Liesegang H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2014) Genomic and Applied Microbiology, Goettingen
            Genomics Laboratory, Georg-August University Goettingen, 
            Grisebachstr.8, Goettingen, Lower Saxony D-37077, Germany
REFERENCE   3  (bases 1 to 4423)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4423)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAR-2014) Genomic and Applied Microbiology, Goettingen Genomics 
            Laboratory, Georg-August University Goettingen, Grisebachstr.8, 
            Goettingen, Lower Saxony D-37077, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..119
                     /label=AmpR promoter
     CDS             120..977
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1151..1739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2004..2774)
                     /codon_start=1
                     /gene="kan"
                     /product="kanamycin nucleotidyltransferase"
                     /label=kan
                     /protein_id="AHZ90956.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGR
                     QTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHG
                     QFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTT
                     FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSD
                     SEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     gene            complement(2004..2774)
                     /gene="kan"
                     /label=kan
     oriT            3066..3175
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(3206..4207)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     rep_origin      complement(4359..4385)
                     /direction=LEFT
                     /label=oriU
                     /note="oriU"
ORIGIN
        1 gatccggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
       61 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      121 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      181 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      241 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      301 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      361 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      421 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      481 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      541 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      601 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      661 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      721 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      781 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
      841 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
      901 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
      961 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1021 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1081 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1141 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1201 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1261 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1321 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1381 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1441 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1501 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1561 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1621 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1681 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1741 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1801 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     1861 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     1921 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagacgcg
     1981 tacgtaccta ggcgcgccat cgttcaaaat ggtatgcgtt ttgacacatc cactatatat
     2041 ccgtgtcgtt ctgtccactc ctgaatccca ttccagaaat tctctagcga ttccagaagt
     2101 ttctcagagt cggaaagttg accagacatt acgaactggc acagatggtc ataacctgaa
     2161 ggaagatctg attgcttaac tgcttcagtt aagaccgaag cgctcgtcgt ataacagatg
     2221 cgatgatgca gaccaatcaa catggcacct gccattgcta cctgtacagt caaggatggt
     2281 agaaatgttg tcggtccttg cacacgaata ttacgccatt tgcctgcata ttcaaacagc
     2341 tcttctacga taagggcaca aatcgcatcg tggaacgttt gggcttctac cgatttagca
     2401 gtttgataca ctttctctaa gtatccacct gaatcataaa tcggcaaaat agagaaaaat
     2461 tgaccatgtg taagcggcca atctgattcc acctgagatg cataatctag tagaatctct
     2521 tcgctatcaa aattcacttc caccttccac tcaccggttg tccattcatg gctgaactct
     2581 gcttcctctg ttgacatgac acacatcatc tcaatatccg aatagggccc atcagtctga
     2641 cgaccaagag agccataaac accaatagcc ttaacatcat ccccatattt atccaatatt
     2701 cgttccttaa tttcatgaac aatcttcatt ctttcttctc tagtcattat tattggtcca
     2761 ttcactattc tcattccctt ttcagataat tttagatttg cttttctaaa taagaatatt
     2821 tggagagcac cgttcttatt cagctattaa taactcgtct tcctaagcat ccttcaatcc
     2881 ttttaataac aattatagca tctaatcttc aacaaactgg cccgtttgtt gaacgcgcgc
     2941 gtacgactag tcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca
     3001 cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt
     3061 cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc
     3121 ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccggctacc
     3181 gccggcgtaa cagatgtgat cactactctt taataaaata atttttccgt tcccaattcc
     3241 acattgcaat aatagaaaat ccatcttcat cggctttttc gtcatcatct gtatgaatca
     3301 aatcgccttc ttctgtgtca tcaaggttta attttttatg tatttctttt aacaaaccac
     3361 cataggagat taacctttta cggtgtaaac cttcctccaa atcagacaaa cgtttcaaat
     3421 tcttttcttc atcatcggtc ataaaatccg tatcctttac aggatatttt gcagtttcgt
     3481 caattgccga ttgtatatcc gatttatatt tatttttcgg tcgaatcatt tgaactttta
     3541 catttggatc atagtctaat ttcattgcct ttttccaaaa ttgaatccat tgtttttgat
     3601 tcacgtagtt ttctgtattc ttaaaataag ttggttccac acataccaat acatgcatgt
     3661 gctgattata agaattatct ttattattta ttgtcacttc cgttgcacgc ataaaaccaa
     3721 caagattttt attaattttt ttatattgca tcattcggcg aaatccttga gccatatctg
     3781 acaaactctt atttaattct tcgccatcat aaacattttt aactgttaat gtgagaaaca
     3841 accaacgaac tgttggcttt tgtttaataa cttcagcaac aaccttttgt gactgaatgc
     3901 catgtttcat tgctctcctc cagttgcaca ttggacaaag cctggattta caaaaccaca
     3961 ctcgatacaa ctttctttcg cctgtttcac gattttgttt atactctaat atttcagcac
     4021 aatcttttac tctttcagcc tttttaaatt caagaatatg cagaagttca aagtaatcaa
     4081 cattagcgat tttcttttct ctccatggtc tcacttttcc actttttgtc ttgtccacta
     4141 aaacccttga tttttcatct gaataaatgc tactattagg acacataata ttaaaagaaa
     4201 cccccatcta tttagttatt tgtttagtca cttataactt taacagatgg ggtttttctg
     4261 tgcaaccaat tttaagggtt ttcaatactt taaaacacat acataccaac acttcaacgc
     4321 acctttcagc aactaaaata aaaatgacgt tatttctata tgtatcaaga taagaaagaa
     4381 caagttcaaa accatcaaaa aaagacacct tttcaggtgc ttg
//
