LOCUS       62056_21830        8629 bp DNA     circular SYN 06-APR-2019
DEFINITION  Transformation vector pUbSV, complete sequence.
ACCESSION   MK424966
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Luo J, Shen P, Chen J.
  TITLE     A modular toolset of phiC31-based fluorescent protein tagging 
            vectors for Drosophila
  JOURNAL   Fly (Austin) (2019) In press
  PUBMED    30885036
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   Luo J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2019) Model Animal Research Center, Nanjing 
            University, 12 Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China
REFERENCE   3  (bases 1 to 8629)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JAN-2019) Model Animal Research Center, Nanjing University, 12 
            Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China"
FEATURES             Location/Qualifiers
     source          1..8629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    667..2546
                     /label=vermilion
                     /note="vermilion"
     protein_bind    2553..2586
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2587..3017
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     polyA_signal    complement(3028..3162)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3434..3454)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(3584..3649)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     misc_feature    complement(3727..3744)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    complement(3745..3747)
                     /label=ATG
                     /note="ATG"
     promoter        complement(3750..5569)
                     /label=Polyubiquitin
                     /note="Promoter of polyubiqutin gene of Drosophila
                     melanogaster"
     misc_feature    5684..6114
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    6153..6222
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(6441..6459)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6480..6496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6504..6520)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6528..6558)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6573..6594)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6882..7470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7644..8501)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8502..8606)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggccccattt gtgtgcgccg cactttcacc tcaagtgatt gataattccc agcctatctg
      721 gcagtgccca tcgcccagat caccgactgt gcaatcagtc ggaactggag ctctctcgct
      781 ctgttatcgg ttcgctgggg tctcatctcc ggtccgctgg cggagatcag ttcgccagca
      841 tccgccgctc gaggagtcac gatctgatct gagctgtgca ccatgagctg tccctatgca
      901 ggaaacgggt gagcaccagc acgtgctgtc caggaatgcc aatcgatctt cagttctgcg
      961 attcaattca aacccataca gaaacgatca cgatgattcg gcggtgccat taaccacgga
     1021 agtgggcaaa atctatggag agtatctgat gctggacaaa ctgctggatg cccagtgtat
     1081 gctgtccgag gaggacaagc gacccgtgca cgatgagcat ctgttcatca tcacgcacca
     1141 gggtgagtag gtttacaact ttgatgacaa cactcaatgg catttaagta ccttcgccac
     1201 agcctacgag ctttggttca agcagatcat ctttgagttc gactccatac gagacatgtt
     1261 ggatgcagag gtcatcgatg aaaccaagac gctggagatt gtcaagcgac tgaaccgagt
     1321 ggttctgatt ctaaaagtga gtgctttctg aatctcttac caaaatccgt ttataacttc
     1381 ctttgtacag ctcctggtgg accaagtgcc cattctggag accatgaccc cgctagactt
     1441 catggacttc cgcaagtacc tggcacccgc atctggtttt cagtcgctgc agttccgttt
     1501 gatcgagaac aagctgggag ttctgacaga gcagcgggtg agatacaacc agaagtactc
     1561 ggatgtcttt agcgacgagg aggcgcggaa ctcgattcgc aactcggaga aagatccctc
     1621 gctactggag ctagtgcagc gatggctgga gaggacgccc ggactggagg agagtggctt
     1681 caacttctgg gccaagtttc aggagagcgt cgatcgattc ctggaggcgc aggtacagag
     1741 cgccatggag gagcccgtgg agaaggcgaa aaactaccgc ctcatggaca ttgagaagcg
     1801 acgcgaggtg tatcgctcca tctttgatcc ggcagtgcac gatgcactgg tgcgtcgtgg
     1861 ggatcgccgg tttagccatc gtgcccttca gggagccatc atgatcacct tctataggga
     1921 tgaacccagg ttcagccaac cacaccagtt gctcaccctg ctcatggaca tcgactcgtt
     1981 aataaccaag tggagatgta agtattgcat tctttgatac tcttttataa atatatctta
     2041 tgtttaagac tggttttcct aaccaaatac tttctattcc cgccgcagac aatcacgtga
     2101 tcatggtgca acgcatgatt ggatcccaac agttgggcac tggtggctcg tctggatatc
     2161 aatatctgcg ctccactctc aggtgatcat cgcagatgtg attatatcgg ggatcaatga
     2221 actcaaactg ttctcccttt gttttttttt ggtttcagtg atcggtacaa ggtgtttctg
     2281 gatctgttca atctgtccac ttttctgatt ccccgcgagg cgattccacc gctggacgag
     2341 accattcgca agaaactgat caacaaaagt gtctgacaat cggcagggta tccaattcgt
     2401 caatgtttgg ctatgcgttg tttgttctgc ctactgtttt gtcgttttgg tgtaataaaa
     2461 ttacttgttt agtctttgtt atcacatttg atgtgttcct tttctttatg tctgacatat
     2521 aatacatata acataacaaa ataaatgcta gtataacttc gtataatgta tgctatacga
     2581 agttattggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     2641 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     2701 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     2761 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     2821 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaagaat
     2881 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     2941 ataccttttc ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg
     3001 gtgtgccaac caacaacacg cgtactggat ccagacatga taagatacat tgatgagttt
     3061 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     3121 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     3181 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     3241 tacaaatgtg gtatggctga ttatgatctc tagtcaaggc actatacatc aaatattttt
     3301 ccataatttt cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag
     3361 caagagttct attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt
     3421 ccttggggtc ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag
     3481 tagcctcatc atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg
     3541 cattccacca ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa
     3601 ttagaatcag tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt
     3661 aaatctctgt aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa
     3721 agatcctcta gaggtaccag atctcatggt ggattattct gcgggcagaa aatagagatg
     3781 tggaaaatta gtacgaaatc aaatgagttt cgttgaaatt acaaaactat tgaaactaac
     3841 ttcctggctg gggaataaaa atgggaaact tatttatcga cgccaacttt gttgagaaac
     3901 ccctattaac cctctacgaa tattggaaca aaggaaagcg aagaaacagg gaacaaaggg
     3961 tagttgagaa acctttgttc cgttgcttcg tcatcgtttt cgtgttgcga gtgtgtgcat
     4021 gtatatatac acagctgaaa tgcatgcata cacattattt tgtgtgtata tggtgacgtc
     4081 acaactacaa gcaataagaa attttcagac gtggctttgt ttaagcaacc tactctattt
     4141 cagctaaaaa taagtggatt tcgttggtaa aatacttcaa ttaactaaac aactaactaa
     4201 taacatgcac acaaatgctc gagtgcgttc gtgatttctc gaattttcaa atgcgtcact
     4261 gcgaatttca caatttgcca ataaatcttg gcgaaaatca acacgcaagt tttatttata
     4321 gatttgtttg cgttttgatg ccaattcatt gggaaaacga gatgcgtggc tgccaatttc
     4381 ttattttgta attacgtaga gcgttgaata aaaaaaaatg gccgaacaaa gaccttgaaa
     4441 tgcagttttt cttgaaatta ctcaacgtct tgttgctctt attactaatt ggtaacagcg
     4501 agttaaaaac ttacgtttct tgtgactttc gagaatgttc ttttaattgt actttaatca
     4561 ccaacaatta agtataaatt tttcgctgat tgcgctttac tttctgcttg tacttgctgc
     4621 tgcaaatgtc aattggtttt gaaggcgacc gttcgcgaac gctgtttata taccttcggt
     4681 gtccgttgaa aatcactaaa aaataccgta gtgttcgtaa cactttagta cagagaaaaa
     4741 aaattgtgcc gaaatgtttt tgatacgtac gaataccttg tattaaaatt ttttatgatt
     4801 tctatgtatc actttttttt tgtgtttttc gtttaaactc accacagtac aaaacaataa
     4861 aatattttta agacaatttc aaattgagac ctttctcgta ctgacttgac cggctgaatg
     4921 aggatttcta cctagacgac ctacttctta ccatgacatt gaatgcaatg ccacctttga
     4981 tctaaactta caaaagtcca aggcttgtta ggattggtgt tttggaattt aagacccttg
     5041 ttagtttgct tttgaaatag cactgtcttc tctaccggct ataattttga aactcgcagc
     5101 ttgactggaa atttaaaaag taattctgtg taggtaaagg gtgttttaaa agtgtgatgt
     5161 gttgagcgtt gcggcaacga ctgctattta tgtatatatt ttcaaaactt attgtttttg
     5221 aagtgtttta aatggagcta tctggcaacg ctgcgcataa tcttacacaa gcttttctta
     5281 atccattttt aagtgaaatt tgtttatact ctttcggcaa ataattgtta aatcgcttta
     5341 agtgggctta catctggata agtaatgaaa acctgcatat tataatatta aaacatataa
     5401 tccactgtgc tttccccgtg tgtggccata tacctaaaaa agtttatttt cgcagagccc
     5461 cgcacggtca cactacggtt cggcgatttt cgattttgga cagtactgat tgcaagcgca
     5521 ccgaaagcaa aatggagctg gagattttga acgcgaagaa cagcaagccg tacggcaagg
     5581 tgaaggtgcc ctccggcgcc acgcccatcg gcgatctgcg cgccctaatt cacaagaccc
     5641 tgaagcagac ctgcaggcat gcaagcttgg atcctcaagg ccttggccac gtaataagtg
     5701 tgcgttgaat ttattcgcaa aaacattgca tattttcggc aaagtaaaat tttgttgcat
     5761 accttatcaa aaaataagtg ctgcatactt tttagagaaa ccaaataatt ttttattgca
     5821 tacccgtttt taataaaata cattgcatac cctcttttaa taaaaaatat tgcatacttt
     5881 gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat acccgttttt
     5941 aataaaatac attgcatacc ctcttttaat aaagaatatt gcatacgttg acgaaacaaa
     6001 ttttcgttgc atacccaata aaagattatt atattgcata ccttttcttg ccataccatt
     6061 tagccgatca attgtgctcg gcaacagtat atttgtggtg tgccaaccaa caacactaga
     6121 gatccactag tgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc
     6181 gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat catgatgaac
     6241 gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc
     6301 tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt
     6361 gcggatacgc ggggcagcgt cagcgggttc tcgacggtca cggcgggcat gtcgacacta
     6421 gttctagcca gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg
     6481 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6541 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6601 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6661 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6721 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6781 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7321 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7561 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7621 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8581 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
