LOCUS       62056_21825        9359 bp DNA     circular SYN 06-APR-2019
DEFINITION  Transformation vector pUbKV, complete sequence.
ACCESSION   MK424967
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Luo J, Shen P, Chen J.
  TITLE     A modular toolset of phiC31-based fluorescent protein tagging 
            vectors for Drosophila
  JOURNAL   Fly (Austin) (2019) In press
  PUBMED    30885036
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   Luo J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2019) Model Animal Research Center, Nanjing 
            University, 12 Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China
REFERENCE   3  (bases 1 to 9359)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JAN-2019) Model Animal Research Center, Nanjing University, 12 
            Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China"
FEATURES             Location/Qualifiers
     source          1..9359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    667..2546
                     /label=vermilion
                     /note="vermilion"
     protein_bind    2553..2586
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2587..3017
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     misc_feature    complement(3027..4407)
                     /label=K10 polyA signal sequence
                     /note="K10 polyA signal sequence"
     misc_feature    complement(4457..4474)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    complement(4475..4477)
                     /label=ATG
                     /note="ATG"
     promoter        complement(4480..6299)
                     /label=Polyubiquitin
                     /note="Promoter of polyubiqutin gene of Drosophila
                     melanogaster"
     misc_feature    6414..6844
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    6883..6952
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(7171..7189)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7210..7226)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7234..7250)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7258..7288)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7303..7324)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7612..8200)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8374..9231)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9232..9336)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggccccattt gtgtgcgccg cactttcacc tcaagtgatt gataattccc agcctatctg
      721 gcagtgccca tcgcccagat caccgactgt gcaatcagtc ggaactggag ctctctcgct
      781 ctgttatcgg ttcgctgggg tctcatctcc ggtccgctgg cggagatcag ttcgccagca
      841 tccgccgctc gaggagtcac gatctgatct gagctgtgca ccatgagctg tccctatgca
      901 ggaaacgggt gagcaccagc acgtgctgtc caggaatgcc aatcgatctt cagttctgcg
      961 attcaattca aacccataca gaaacgatca cgatgattcg gcggtgccat taaccacgga
     1021 agtgggcaaa atctatggag agtatctgat gctggacaaa ctgctggatg cccagtgtat
     1081 gctgtccgag gaggacaagc gacccgtgca cgatgagcat ctgttcatca tcacgcacca
     1141 gggtgagtag gtttacaact ttgatgacaa cactcaatgg catttaagta ccttcgccac
     1201 agcctacgag ctttggttca agcagatcat ctttgagttc gactccatac gagacatgtt
     1261 ggatgcagag gtcatcgatg aaaccaagac gctggagatt gtcaagcgac tgaaccgagt
     1321 ggttctgatt ctaaaagtga gtgctttctg aatctcttac caaaatccgt ttataacttc
     1381 ctttgtacag ctcctggtgg accaagtgcc cattctggag accatgaccc cgctagactt
     1441 catggacttc cgcaagtacc tggcacccgc atctggtttt cagtcgctgc agttccgttt
     1501 gatcgagaac aagctgggag ttctgacaga gcagcgggtg agatacaacc agaagtactc
     1561 ggatgtcttt agcgacgagg aggcgcggaa ctcgattcgc aactcggaga aagatccctc
     1621 gctactggag ctagtgcagc gatggctgga gaggacgccc ggactggagg agagtggctt
     1681 caacttctgg gccaagtttc aggagagcgt cgatcgattc ctggaggcgc aggtacagag
     1741 cgccatggag gagcccgtgg agaaggcgaa aaactaccgc ctcatggaca ttgagaagcg
     1801 acgcgaggtg tatcgctcca tctttgatcc ggcagtgcac gatgcactgg tgcgtcgtgg
     1861 ggatcgccgg tttagccatc gtgcccttca gggagccatc atgatcacct tctataggga
     1921 tgaacccagg ttcagccaac cacaccagtt gctcaccctg ctcatggaca tcgactcgtt
     1981 aataaccaag tggagatgta agtattgcat tctttgatac tcttttataa atatatctta
     2041 tgtttaagac tggttttcct aaccaaatac tttctattcc cgccgcagac aatcacgtga
     2101 tcatggtgca acgcatgatt ggatcccaac agttgggcac tggtggctcg tctggatatc
     2161 aatatctgcg ctccactctc aggtgatcat cgcagatgtg attatatcgg ggatcaatga
     2221 actcaaactg ttctcccttt gttttttttt ggtttcagtg atcggtacaa ggtgtttctg
     2281 gatctgttca atctgtccac ttttctgatt ccccgcgagg cgattccacc gctggacgag
     2341 accattcgca agaaactgat caacaaaagt gtctgacaat cggcagggta tccaattcgt
     2401 caatgtttgg ctatgcgttg tttgttctgc ctactgtttt gtcgttttgg tgtaataaaa
     2461 ttacttgttt agtctttgtt atcacatttg atgtgttcct tttctttatg tctgacatat
     2521 aatacatata acataacaaa ataaatgcta gtataacttc gtataatgta tgctatacga
     2581 agttattggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     2641 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     2701 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     2761 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     2821 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaagaat
     2881 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     2941 ataccttttc ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg
     3001 gtgtgccaac caacaacacg cgtacttcag ccaatccgcc gcaccctcag ctccaaagca
     3061 cgcatatgac ccgtgagcag gcagagaaac gcaggcggat aggagtcact agcgcagttc
     3121 tgtatagcct gcactatcaa gccgaagagc tggtagatat tgatgagcac atagtttctg
     3181 gtggagtgca tccagtcaac gccgaaccag gccggataca ggagctctcg atccgggcga
     3241 acgaccacgc ggacgcaact caaagtagtg ccaaatacga aaatactggc aaaggtgcgg
     3301 aaagtgccct gtgcgatatt cacttccttc ctcaaggcag aaatctcctc gggatcgccc
     3361 accagccggg ctctagcgtc catgagcctt aggagagagc gaatctcatg gagctgcttc
     3421 ctcaccatgt acacattcgc aaacttcaaa ttggccacaa tatcggtaat agttatggtc
     3481 aggttctcgc acaatccggc catgttggtg gtgaacagca gcctggccag caagctcaag
     3541 ggaaacagca cggtgaccac caagttgacc gtaatggagt ataccacgta aagcaggctt
     3601 gaaacgcccg gaggacgcat caggccagtg agctcccaaa cgcgccagtg gtagtacaca
     3661 gcactgtggg tgttcagttt gaaatcctct tgcttctcca ttgtctcggt tacctttggt
     3721 caaatccatg ggttctattg cctatatact cttgcgatta ccagtgattg cgctattagc
     3781 tattagatgg attgttggcc aaacttgtcg cttaagtggc tgggaattgt aaccgtaggc
     3841 ccgagtgtaa tgatccccca taaaaagttt tcgcaatgcc tttatttttt gttgcaaatc
     3901 tctctttatt ctgcggtatt cttcattatt gcggggatgg ggaaagtgtt tatatagaag
     3961 caacttacga ttgaacccaa atgcacctga caagcaaggt caaagggcca gatttttaaa
     4021 tatattattt agtcttagga ctctctattt gcaattaaat tactttgcta cctgagggtt
     4081 aaatcttccc cattgataat aataattcca ctatatgttc aattgggttt caccgcgctt
     4141 agttacatga cgagccctaa tgagccgtcg gtggtctata aactgtgcct tacaaatact
     4201 tgcaactctt ctcgttttga agtcagcaga gttattgcta attgctaatt gctaattgct
     4261 tttaactgat ttcttcgaaa ttggtgctat gtttatggcg ctattaacaa gtatgaatgt
     4321 caggtttaac caggggatgc ttaattgtgt tctcaacttc aaaggcagaa atgtttactc
     4381 ttgaccatgg gtttaggtat aatgttatca agctcctcga gttaacgtta cgttaacgtt
     4441 aacgttcgag gtcgactcta gaggtaccag atctcatggt ggattattct gcgggcagaa
     4501 aatagagatg tggaaaatta gtacgaaatc aaatgagttt cgttgaaatt acaaaactat
     4561 tgaaactaac ttcctggctg gggaataaaa atgggaaact tatttatcga cgccaacttt
     4621 gttgagaaac ccctattaac cctctacgaa tattggaaca aaggaaagcg aagaaacagg
     4681 gaacaaaggg tagttgagaa acctttgttc cgttgcttcg tcatcgtttt cgtgttgcga
     4741 gtgtgtgcat gtatatatac acagctgaaa tgcatgcata cacattattt tgtgtgtata
     4801 tggtgacgtc acaactacaa gcaataagaa attttcagac gtggctttgt ttaagcaacc
     4861 tactctattt cagctaaaaa taagtggatt tcgttggtaa aatacttcaa ttaactaaac
     4921 aactaactaa taacatgcac acaaatgctc gagtgcgttc gtgatttctc gaattttcaa
     4981 atgcgtcact gcgaatttca caatttgcca ataaatcttg gcgaaaatca acacgcaagt
     5041 tttatttata gatttgtttg cgttttgatg ccaattcatt gggaaaacga gatgcgtggc
     5101 tgccaatttc ttattttgta attacgtaga gcgttgaata aaaaaaaatg gccgaacaaa
     5161 gaccttgaaa tgcagttttt cttgaaatta ctcaacgtct tgttgctctt attactaatt
     5221 ggtaacagcg agttaaaaac ttacgtttct tgtgactttc gagaatgttc ttttaattgt
     5281 actttaatca ccaacaatta agtataaatt tttcgctgat tgcgctttac tttctgcttg
     5341 tacttgctgc tgcaaatgtc aattggtttt gaaggcgacc gttcgcgaac gctgtttata
     5401 taccttcggt gtccgttgaa aatcactaaa aaataccgta gtgttcgtaa cactttagta
     5461 cagagaaaaa aaattgtgcc gaaatgtttt tgatacgtac gaataccttg tattaaaatt
     5521 ttttatgatt tctatgtatc actttttttt tgtgtttttc gtttaaactc accacagtac
     5581 aaaacaataa aatattttta agacaatttc aaattgagac ctttctcgta ctgacttgac
     5641 cggctgaatg aggatttcta cctagacgac ctacttctta ccatgacatt gaatgcaatg
     5701 ccacctttga tctaaactta caaaagtcca aggcttgtta ggattggtgt tttggaattt
     5761 aagacccttg ttagtttgct tttgaaatag cactgtcttc tctaccggct ataattttga
     5821 aactcgcagc ttgactggaa atttaaaaag taattctgtg taggtaaagg gtgttttaaa
     5881 agtgtgatgt gttgagcgtt gcggcaacga ctgctattta tgtatatatt ttcaaaactt
     5941 attgtttttg aagtgtttta aatggagcta tctggcaacg ctgcgcataa tcttacacaa
     6001 gcttttctta atccattttt aagtgaaatt tgtttatact ctttcggcaa ataattgtta
     6061 aatcgcttta agtgggctta catctggata agtaatgaaa acctgcatat tataatatta
     6121 aaacatataa tccactgtgc tttccccgtg tgtggccata tacctaaaaa agtttatttt
     6181 cgcagagccc cgcacggtca cactacggtt cggcgatttt cgattttgga cagtactgat
     6241 tgcaagcgca ccgaaagcaa aatggagctg gagattttga acgcgaagaa cagcaagccg
     6301 tacggcaagg tgaaggtgcc ctccggcgcc acgcccatcg gcgatctgcg cgccctaatt
     6361 cacaagaccc tgaagcagac ctgcaggcat gcaagcttgg atcctcaagg ccttggccac
     6421 gtaataagtg tgcgttgaat ttattcgcaa aaacattgca tattttcggc aaagtaaaat
     6481 tttgttgcat accttatcaa aaaataagtg ctgcatactt tttagagaaa ccaaataatt
     6541 ttttattgca tacccgtttt taataaaata cattgcatac cctcttttaa taaaaaatat
     6601 tgcatacttt gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat
     6661 acccgttttt aataaaatac attgcatacc ctcttttaat aaagaatatt gcatacgttg
     6721 acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata ccttttcttg
     6781 ccataccatt tagccgatca attgtgctcg gcaacagtat atttgtggtg tgccaaccaa
     6841 caacactaga gatccactag tgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg
     6901 gtgccagggc gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat
     6961 catgatgaac gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa
     7021 gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc
     7081 gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc tcgacggtca cggcgggcat
     7141 gtcgacacta gttctagcca gcttttgttc cctttagtga gggttaattt cgagcttggc
     7201 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7261 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7321 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7381 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7441 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7501 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7561 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7621 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7681 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7741 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7801 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7861 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7921 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7981 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8041 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8101 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8161 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8221 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8281 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8341 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8401 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8461 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8521 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8581 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8641 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8701 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8761 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8821 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8881 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8941 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9001 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9061 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9121 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9181 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9241 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9301 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
