LOCUS       40924_45828        7055 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transposon delivery vector pUTkm1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   de Lorenzo V, Herrero M, Jakubzik U, Timmis KN.
  TITLE     Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter 
            probing, and chromosomal insertion of cloned DNA in gram-negative 
            eubacteria
  JOURNAL   J. Bacteriol. 172 (11), 6568-6572 (1990)
  PUBMED    2172217
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Tombolini R, Jansson J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-1998) Department of Biochemistry, Stockholm 
            University, Svante Arrhenius vag. 10-12, Stockholm SE-10691, Sweden
REFERENCE   3  (bases 1 to 7055)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7055)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "1990"; volume: "172"; issue: "11"; pages: 
            "6568-6572"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-1998) Department of Biochemistry, Stockholm University, 
            Svante Arrhenius vag. 10-12, Stockholm SE-10691, Sweden"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(314..1171)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1172..1276)
                     /label=AmpR promoter
     CDS             complement(1326..2753)
                     /codon_start=1
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     CDS             3394..4206
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     protein_bind    complement(4718..4734)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(4769..5157)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(5778..6146)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(6179..6288)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gccccgtggc cgggggactg ttgggcgcca tctccttgct gcctcgcgcg tttcggtgat
       61 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      181 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
      241 cagagcagat tgtactgaga gtgcaccata aatcaatcta aagtatatat gagtaaactt
      301 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
      361 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
      421 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
      481 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
      541 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
      601 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
      661 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
      721 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
      781 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
      841 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
      901 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
      961 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1021 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1081 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     1141 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     1201 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     1261 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcagatctg caggtcgacc
     1321 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
     1381 ctttgcaggg cttcccaacc ttcccagagg gcgccccagc tggcaattcc ggttcgcttg
     1441 ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct
     1501 ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg
     1561 gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc
     1621 gccctgagtg cttgcggcag cgtgaagctt tctctgagct gtaacagcct gaccgcaaca
     1681 aacgagagga tcgagaccat ccgctccaga ttatccggct cctccatgcg ttgcctctcg
     1741 gctcctgctc cggttttcca tgccttatgg aactcctcga tccgccagcg atgggtataa
     1801 atgtcgatga cgcgcaaggc ttgggctagc gactcgaccg gttcgctggt cagcaacaac
     1861 catttcaacg gggtctcacc cttgggcggg ttaatctcct cggccagcac cgcgttgagc
     1921 gtgatattcc cctgttttag cgtgatgcgc ccactgcgca ggctcaagct cgccttgcgg
     1981 gctggtcgat ttttacgttt accgcgttta tccaccacgc ccttttgcgg aatgctgatc
     2041 tgatagccac ccaactccgg ttggttcttc agatggtcgt acagatacaa cccagactct
     2101 acgtccttgc gtgggtgctt ggagcgcacc acgaagcgct cgttatgcgc cagtttgtcc
     2161 tgcagataag catgaatatc ggcttcgcgg tcacagaccg caatcacgtt gctcatcatg
     2221 ctgcccatgc gtaaccggct agttgcggcg gctgccagcc atttgccact ctccttttca
     2281 tccgcatcgg cagggtcatc cgggcgcatc caccactcct gatgcagtaa tcctacggtg
     2341 cggaatgtgg tggcctcgag caagagaacg gagtgaaccc accatccgcg ggatttatcc
     2401 tgaatagagc ccagcttgcc aagctcttcg gcgacctggt ggcgataact caaagaggtg
     2461 gtgtcctcaa tggccagcag ttcgggaaac tcctgagcca acttgactgt ttgcatggcg
     2521 ccagcctttc tgatcgcctc ggcagaaacg ttgggattgc ggataaatcg gtaagcgcct
     2581 tcctgcatgg cttcactacc ctctgatgag atggttattg atttaccaga atattttgcc
     2641 aattgggcgg cgacgttaac caagcgggca gtacggcgag gatcacccag cgccgccgaa
     2701 gagaacacag atttagccca gtcggccgca cgatgaagag cagaagttat catgaacgtt
     2761 accatgttag gaggtcacat ggaagatcag atcctggaaa acgggaaagg ttccgttcag
     2821 gacgctactt gtgtacggga tcggtcgacg gatcccaagc ttcttctaga ctgtctcttg
     2881 atcagatctg gccgcctagg ccgaattcga gctcggtacc cggggatccg gctgtaatcc
     2941 gggcagcgca acggaacatt catcagtgta aaaatggaat caataaagcc ctgcgcagcg
     3001 cgcagggtca gcctgaatac gcgtttaatg accagcacag tcgtgatggc aaggtcagaa
     3061 tagcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc ctgaatcgcc
     3121 ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac
     3181 cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc
     3241 gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagcca cgttgtgtct
     3301 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     3361 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
     3421 tgctcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     3481 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     3541 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     3601 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     3661 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
     3721 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     3781 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     3841 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3901 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca
     3961 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     4021 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     4081 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     4141 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     4201 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     4261 cgggacggcg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc
     4321 atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt
     4381 ccacctacaa caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg
     4441 cgattcaggc ctggtatgag tcagcaacac cttcttcacg aggcagacct cagcgctatt
     4501 ctgaccttgc catcacgact gtgctggtca ttaaacgcgt attcaggctg accctgcgcg
     4561 ctgcgcaggg ctttattgat tccattttta cactgatgaa tgttccgttg cgctgcccgg
     4621 attacagccg gatcctctag agtcgacctg caggcatgca agcttcggcc gcctaggccg
     4681 cggccgcact tgtgtataag agtcagaatt ccacaaattg ttatccgctc acaattccac
     4741 atgtggaatt ccacatgtgg aattcccatg tcagccgtta agtgttcctg tgtcactcaa
     4801 aattgctttg agaggctcta agggcttctc agtgcgttac atccctggct tgttgtccac
     4861 aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc ttttttttct tataaaactt
     4921 aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc aaacatgaga
     4981 gcttagtacg tgaaacatga gagcttagta cgttagccat gagagcttag tacgttagcc
     5041 atgagggttt agttcgttaa acatgagagc ttagtacgtt aaacatgaga gcttagtacg
     5101 tgaaacatga gagcttagta cgtactatca acaggttgaa ctgctgatct tcagatcctc
     5161 tacgccggac gcatcgtggc cggatccagc cgaccaggct ttccacgccc gcgtgccgct
     5221 ccatgtcgtt cgcgcggttc tcggaaacgc gctgccgcgt ttcgtgattg tcacgctcaa
     5281 gcccgtagtc ccgttcgagc gtcgcgcaga ggtcagcgag ggcgcggtag gcccgatacg
     5341 gctcatggat ggtgtttcgg gtcgggtgaa tcttgttgat ggcgatatgg atgtgcaggt
     5401 tgtcggtgtc gtgatgcacg gcactgacgc gctgatgctc ggcgaagcca agcccagcgc
     5461 agatgcggtc ctcaatcgcg cgcaacgtct ccgcgtcggg cttctctccc gcgcggaagc
     5521 taaccagcag gtgataggtc ttgtcggcct cggaacgggt gttgccgtgc tgggtcgcca
     5581 tcacctcggc catgacagcg ggcagggtgt ttgcctcgca gttcgtgacg cgcacgtgac
     5641 ccaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc gcgaagtcgc
     5701 tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc cggccgtttt
     5761 agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat gaccttgcca
     5821 agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc atcaccgaac
     5881 cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag gcggcccagg
     5941 tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc cgtgattttg
     6001 tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc cgccgccttt
     6061 tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg gctgcccttc
     6121 ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc
     6181 ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg
     6241 aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt
     6301 tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa
     6361 aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa
     6421 aagcgctgct tccctgctgt tttgtggaat atctaccgac tggaaacagg caaatgcagg
     6481 aaattactga actgagggga caggcgagag acgatgccaa agagctacac cgacgagctg
     6541 gccgagtggg ttgaatcccg cgcggccaag aagcgccggc gtgatgaggc tgcggttgcg
     6601 ttcctggcgg tgagggcgga tgtcgaggcg gcgttagcgt ccggctatgc gctcgtcacc
     6661 atttgggagc acatgcggga aacggggaag gtcaagttct cctacgagac gttccgctcg
     6721 cacgccaggc ggcacatcaa ggccaagccc gccgatgtgc ccgcaccgca ggccaaggct
     6781 gcggaacccg cgccggcacc caagacgccg gagccacggc ggccgaagca ggggggcaag
     6841 gctgaaaagc cggcccccgc tgcggccccg accggcttca ccttcaaccc aacaccggac
     6901 aaaaaggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     6961 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     7021 catgagcgct tgtttcggcg tgggtatggt ggcag
//
