LOCUS       40924_45843        9410 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Insertion vector pUTTnsTet, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Li R, Wang G, Shen B, Wang R, Song Y, Li S, Jiang J.
  TITLE     Random transposon vectors pUTTns for the markerless integration of 
            exogenous genes into gram-negative eubacteria chromosomes
  JOURNAL   J. Microbiol. Methods 79 (2), 220-226 (2009)
  PUBMED    19778558
REFERENCE   2  (bases 1 to 9410)
  AUTHORS   Li R, Jiang J, Li S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2009) College of Life Science, Nanjing 
            Agricultural University, 6# Tongwei Road, Nanjing, Jiangsu 210095, 
            China
REFERENCE   3  (bases 1 to 9410)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9410)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2009"; volume: "79"; issue: "2"; pages: 
            "220-226"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JUL-2009) College of Life Science, Nanjing Agricultural 
            University, 6# Tongwei Road, Nanjing, Jiangsu 210095, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(314..1171)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1172..1276)
                     /label=AmpR promoter
     CDS             complement(1326..2753)
                     /codon_start=1
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     misc_feature    2871..2889
                     /label=Tn5 IE end
                     /note="Tn5 IE end"
     CDS             complement(3256..4674)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        5114..5142
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             5190..6377
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     misc_feature    6581..7012
                     /label=similar to sacB
                     /note="similar to sacB"
     misc_feature    7043..7061
                     /label=Tn5 OE end
                     /note="Tn5 OE end"
     protein_bind    complement(7073..7089)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(7124..7512)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(8133..8501)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(8534..8643)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gccccgtggc cgggggactg ttgggcgcca tctccttgct gcctcgcgcg tttcggtgat
       61 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      181 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
      241 cagagcagat tgtactgaga gtgcaccata aatcaatcta aagtatatat gagtaaactt
      301 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
      361 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
      421 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
      481 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
      541 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
      601 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
      661 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
      721 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
      781 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
      841 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
      901 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
      961 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1021 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1081 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     1141 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     1201 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     1261 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcagatctg caggtcgacc
     1321 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
     1381 ctttgcaggg cttcccaacc ttcccagagg gcgccccagc tggcaattcc ggttcgcttg
     1441 ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct
     1501 ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg
     1561 gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc
     1621 gccctgagtg cttgcggcag cgtgaagctt tctctgagct gtaacagcct gaccgcaaca
     1681 aacgagagga tcgagaccat ccgctccaga ttatccggct cctccatgcg ttgcctctcg
     1741 gctcctgctc cggttttcca tgccttatgg aactcctcga tccgccagcg atgggtataa
     1801 atgtcgatga cgcgcaaggc ttgggctagc gactcgaccg gttcgctggt cagcaacaac
     1861 catttcaacg gggtctcacc cttgggcggg ttaatctcct cggccagcac cgcgttgagc
     1921 gtgatattcc cctgttttag cgtgatgcgc ccactgcgca ggctcaagct cgccttgcgg
     1981 gctggtcgat ttttacgttt accgcgttta tccaccacgc ccttttgcgg aatgctgatc
     2041 tgatagccac ccaactccgg ttggttcttc agatggtcgt acagatacaa cccagactct
     2101 acgtccttgc gtgggtgctt ggagcgcacc acgaagcgct cgttatgcgc cagtttgtcc
     2161 tgcagataag catgaatatc ggcttcgcgg tcacagaccg caatcacgtt gctcatcatg
     2221 ctgcccatgc gtaaccggct agttgcggcg gctgccagcc atttgccact ctccttttca
     2281 tccgcatcgg cagggtcatc cgggcgcatc caccactcct gatgcagtaa tcctacggtg
     2341 cggaatgtgg tggcctcgag caagagaacg gagtgaaccc accatccgcg ggatttatcc
     2401 tgaatagagc ccagcttgcc aagctcttcg gcgacctggt ggcgataact caaagaggtg
     2461 gtgtcctcaa tggccagcag ttcgggaaac tcctgagcca acttgactgt ttgcatggcg
     2521 ccagcctttc tgatcgcctc ggcagaaacg ttgggattgc ggataaatcg gtaagcgcct
     2581 tcctgcatgg cttcactacc ctctgatgag atggttattg atttaccaga atattttgcc
     2641 aattgggcgg cgacgttaac caagcgggca gtacggcgag gatcacccag cgccgccgaa
     2701 gagaacacag atttagccca gtcggccgca cgatgaagag cagaagttat catgaacgtt
     2761 accatgttag gaggtcacat ggaagatcag atcctggaaa acgggaaagg ttccgttcag
     2821 gacgctactt gtgtacggga tcggtcgacg gatcccaagc ttcttctaga ctgtctcttg
     2881 atcagatctg gccgcctagg ccgaattcga gctccttccc aaccttacca gagggcgccc
     2941 cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg
     3001 taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag
     3061 cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgt
     3121 tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa gctcgaggta
     3181 tgggattcac ctttatgttg ataagaaata aaagaaaatg ccaataggat atcggcattt
     3241 tcttttgcgt ttttatttgt taactgttaa ttgtccttgt tcaaggatgc tgtctttgac
     3301 aacagatgtt ttcttgcctt tgatgttcag caggaagctt ggcgcaaacg ttgattgttt
     3361 gtctgcgtag aatcctctgt ttgtcatata gcttgtaatc acgacattgt ttcctttcgc
     3421 ttgaggtaca gcgaagtgtg agtaagtaaa ggttacatcg ttaggatcaa gatccatttt
     3481 taacacaagg ccagttttgt tcagcggctt gtatgggcca gttaaagaat tagaaacata
     3541 accaagcatg taaatatcgt tagacgtaat gccgtcaatc gtcatttttg atccgcggga
     3601 gtcagtgaac aggtaccatt tgccgttcat tttaaagacg ttcgcgcgtt caatttcatc
     3661 tgttactgtg ttagatgcaa tcagcggttt catcactttt ttcagtgtgt aatcatcgtt
     3721 tagctcaatc ataccgagag cgccgtttgc taactcagcc gtgcgttttt tatcgctttg
     3781 cagaagtttt tgactttctt gacggaagaa tgatgtgctt ttgccatagt atgctttgtt
     3841 aaataaagat tcttcgcctt ggtagccatc ttcagttcca gtgtttgctt caaatactaa
     3901 gtatttgtgg cctttatctt ctacgtagtg aggatctctc agcgtatggt tgtcgcctga
     3961 gctgtagttg ccttcatcga tgaactgctg tacattttga tacgtttttc cgtcaccgtc
     4021 aaagattgat ttataatcct ctacaccgtt gatgttcaaa gagctgtctg atgctgatac
     4081 gttaacttgt gcagttgtca gtgtttgttt gccgtaatgt ttaccggaga aatcagtgta
     4141 gaataaacgg atttttccgt cagatgtaaa tgtggctgaa cctgaccatt cttgtgtttg
     4201 gtcttttagg atagaatcat ttgcatcgaa tttgtcgctg tctttaaaga cgcggccagc
     4261 gtttttccag ctgtcaatag aagtttcgcc gactttttga tagaacatgt aaatcgatgt
     4321 gtcatccgca tttttaggat ctccggctaa tgcaaagacg atgtggtagc cgtgatagtt
     4381 tgcgacagtg ccgtcagcgt tttgtaatgg ccagctgtcc caaacgtcca ggccttttgc
     4441 agaagagata tttttaattg tggacgaatc gaattcagga acttgatatt tttcattttt
     4501 ttgctgttca gggatttgca gcatatcatg gcgtgtaata tgggaaatgc cgtatgtttc
     4561 cttatatggc ttttggttcg tttctttcgc aaacgcttga gttgcgcctc ctgccagcag
     4621 tgcggtagta aaggttaata ctgttgcttg ttttgcaaac tttttgatgt tcatcgttca
     4681 tgtctccttt tttatgtact gtgttagcgg tctgcttctt ccagccctcc tgtttgaaga
     4741 tggcaagtta gttacgcaca ataaaaaaag acctaaaata tgtaaggggt gacgccaaag
     4801 tatacacttt gccctttaca cattttaggt cttgcctgct ttatcagtaa caaacccgcg
     4861 cgatttactt ttcgacctca ttctattaga ctctcgtttg gattgcaact ggtctatttt
     4921 cctcttttgt ttgatagaaa atcataaaag gatttgcaga ctacgggcct aaagaactag
     4981 agctcggtac ccggggatcc ggctgtaatc cgggcagcgc aacggaacat tcatcagtgt
     5041 aaaaatggaa tcaataaagc cctgcgcagc gcgcagggtc agcctgaata cgcgtactag
     5101 tgaattctca tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt
     5161 taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc
     5221 tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc
     5281 tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc
     5341 tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg
     5401 gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg
     5461 cgaccacacc cgtcctgtgg atcctctacg ccggacgcat cgtggccggc atcaccggcg
     5521 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     5581 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     5641 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
     5701 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac
     5761 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     5821 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     5881 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
     5941 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
     6001 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
     6061 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     6121 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     6181 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg
     6241 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat
     6301 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
     6361 gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc
     6421 aagaattgga gccaatcaat tgacgtcacg cgtattcagg ctgaccctgc gcgctgcgca
     6481 gggctttatt gattccattt ttacactgat gaatgttccg ttgcgctgcc cggattacag
     6541 ccggatcctc tagagtcgac ctgcaggcat gcgacaacag atgttttctt gcctttgatg
     6601 ttcagcagga agcttggcgc aaacgttgat tgtttgtctg cgtagaatcc tctgtttgtc
     6661 atatagcttg taatcacgac attgtttcct ttcgcttgag gtacagcgaa gtgtgagtaa
     6721 gtaaaggtta catcgttagg atcaagatcc atttttaaca caaggccagt tttgttcagc
     6781 ggcttgtatg ggccagttaa agaattagaa acataaccaa gcatgtaaat atcgttagac
     6841 gtaatgccgt caatcgtcat ttttgatccg cgggagtcag tgaacaggta ccatttgccg
     6901 ttcattttaa agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga tgcaatcagc
     6961 ggtttcatca cttttttcag tgtgtaatca tcgtttagct caatcatacc gagggcccat
     7021 ggcatatgtc cggagcggcc gcacttgtgt ataagagtca gaattccaca aattgttatc
     7081 cgctcacaat tccacatgtg gaattccaca tgtggaattc ccatgtcagc cgttaagtgt
     7141 tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc ttctcagtgc gttacatccc
     7201 tggcttgttg tccacaaccg ttaaacctta aaagctttaa aagccttata tattcttttt
     7261 tttcttataa aacttaaaac cttagaggct atttaagttg ctgatttata ttaattttat
     7321 tgttcaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtta gccatgagag
     7381 cttagtacgt tagccatgag ggtttagttc gttaaacatg agagcttagt acgttaaaca
     7441 tgagagctta gtacgtgaaa catgagagct tagtacgtac tatcaacagg ttgaactgct
     7501 gatcttcaga tcctctacgc cggacgcatc gtggccggat ccagccgacc aggctttcca
     7561 cgcccgcgtg ccgctccatg tcgttcgcgc ggttctcgga aacgcgctgc cgcgtttcgt
     7621 gattgtcacg ctcaagcccg tagtcccgtt cgagcgtcgc gcagaggtca gcgagggcgc
     7681 ggtaggcccg atacggctca tggatggtgt ttcgggtcgg gtgaatcttg ttgatggcga
     7741 tatggatgtg caggttgtcg gtgtcgtgat gcacggcact gacgcgctga tgctcggcga
     7801 agccaagccc agcgcagatg cggtcctcaa tcgcgcgcaa cgtctccgcg tcgggcttct
     7861 ctcccgcgcg gaagctaacc agcaggtgat aggtcttgtc ggcctcggaa cgggtgttgc
     7921 cgtgctgggt cgccatcacc tcggccatga cagcgggcag ggtgtttgcc tcgcagttcg
     7981 tgacgcgcac gtgacccagg cgctcggtct tgccttgctc gtcggtgatg tacttcacca
     8041 gctccgcgaa gtcgctcttc ttgatggagc gcatggggac gtgcttggca atcacgcgca
     8101 ccccccggcc gttttagcgg ctaaaaaagt catggctctg ccctcgggcg gaccacgccc
     8161 atcatgacct tgccaagctc gtcctgcttc tcttcgatct tcgccagcag ggcgaggatc
     8221 gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga gccagagttt cagcaggccg
     8281 cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc ggacgtgctc atagtccacg
     8341 acgcccgtga ttttgtagcc ctggccgacg gccagcaggt aggccgacag gctcatgccg
     8401 gccgccgccg ccttttcctc aatcgctctt cgttcgtctg gaaggcagta caccttgata
     8461 ggtgggctgc ccttcctggt tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt
     8521 acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc
     8581 gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg
     8641 cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct
     8701 gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag
     8761 ggttatgcag cggaaaagcg ctgcttccct gctgttttgt ggaatatcta ccgactggaa
     8821 acaggcaaat gcaggaaatt actgaactga ggggacaggc gagagacgat gccaaagagc
     8881 tacaccgacg agctggccga gtgggttgaa tcccgcgcgg ccaagaagcg ccggcgtgat
     8941 gaggctgcgg ttgcgttcct ggcggtgagg gcggatgtcg aggcggcgtt agcgtccggc
     9001 tatgcgctcg tcaccatttg ggagcacatg cgggaaacgg ggaaggtcaa gttctcctac
     9061 gagacgttcc gctcgcacgc caggcggcac atcaaggcca agcccgccga tgtgcccgca
     9121 ccgcaggcca aggctgcgga acccgcgccg gcacccaaga cgccggagcc acggcggccg
     9181 aagcaggggg gcaaggctga aaagccggcc cccgctgcgg ccccgaccgg cttcaccttc
     9241 aacccaacac cggacaaaaa ggatcctcta cgccggacgc atcgtggccg gcatcaccgg
     9301 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     9361 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
//
