LOCUS       40924_45838        9568 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Insertion vector pUTTnsKm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Li R, Wang G, Shen B, Wang R, Song Y, Li S, Jiang J.
  TITLE     Random transposon vectors pUTTns for the markerless integration of 
            exogenous genes into gram-negative eubacteria chromosomes
  JOURNAL   J. Microbiol. Methods 79 (2), 220-226 (2009)
  PUBMED    19778558
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   Li R, Jiang J, Li S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2009) College of Life Science, Nanjing 
            Agricultural University, 6# Tongwei Road, Nanjing, Jiangsu 210095, 
            China
REFERENCE   3  (bases 1 to 9568)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9568)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2009"; volume: "79"; issue: "2"; pages: 
            "220-226"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JUL-2009) College of Life Science, Nanjing Agricultural 
            University, 6# Tongwei Road, Nanjing, Jiangsu 210095, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(314..1171)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1172..1276)
                     /label=AmpR promoter
     CDS             complement(1326..2753)
                     /codon_start=1
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     misc_feature    2871..2889
                     /label=Tn5 IE end
                     /note="Tn5 IE end"
     CDS             complement(3256..4674)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     CDS             5465..6277
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    6739..7170
                     /label=similar to sacB
                     /note="similar to sacB"
     misc_feature    7171..7200
                     /label=MCS
                     /note="MCS"
     misc_feature    7201..7219
                     /label=Tn5 OE end
                     /note="Tn5 OE end"
     protein_bind    complement(7231..7247)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(7282..7670)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(8291..8659)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(8692..8801)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gccccgtggc cgggggactg ttgggcgcca tctccttgct gcctcgcgcg tttcggtgat
       61 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      181 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
      241 cagagcagat tgtactgaga gtgcaccata aatcaatcta aagtatatat gagtaaactt
      301 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
      361 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
      421 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
      481 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
      541 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
      601 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
      661 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
      721 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
      781 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
      841 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
      901 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
      961 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1021 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1081 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     1141 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     1201 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     1261 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcagatctg caggtcgacc
     1321 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
     1381 ctttgcaggg cttcccaacc ttcccagagg gcgccccagc tggcaattcc ggttcgcttg
     1441 ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct
     1501 ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg
     1561 gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc
     1621 gccctgagtg cttgcggcag cgtgaagctt tctctgagct gtaacagcct gaccgcaaca
     1681 aacgagagga tcgagaccat ccgctccaga ttatccggct cctccatgcg ttgcctctcg
     1741 gctcctgctc cggttttcca tgccttatgg aactcctcga tccgccagcg atgggtataa
     1801 atgtcgatga cgcgcaaggc ttgggctagc gactcgaccg gttcgctggt cagcaacaac
     1861 catttcaacg gggtctcacc cttgggcggg ttaatctcct cggccagcac cgcgttgagc
     1921 gtgatattcc cctgttttag cgtgatgcgc ccactgcgca ggctcaagct cgccttgcgg
     1981 gctggtcgat ttttacgttt accgcgttta tccaccacgc ccttttgcgg aatgctgatc
     2041 tgatagccac ccaactccgg ttggttcttc agatggtcgt acagatacaa cccagactct
     2101 acgtccttgc gtgggtgctt ggagcgcacc acgaagcgct cgttatgcgc cagtttgtcc
     2161 tgcagataag catgaatatc ggcttcgcgg tcacagaccg caatcacgtt gctcatcatg
     2221 ctgcccatgc gtaaccggct agttgcggcg gctgccagcc atttgccact ctccttttca
     2281 tccgcatcgg cagggtcatc cgggcgcatc caccactcct gatgcagtaa tcctacggtg
     2341 cggaatgtgg tggcctcgag caagagaacg gagtgaaccc accatccgcg ggatttatcc
     2401 tgaatagagc ccagcttgcc aagctcttcg gcgacctggt ggcgataact caaagaggtg
     2461 gtgtcctcaa tggccagcag ttcgggaaac tcctgagcca acttgactgt ttgcatggcg
     2521 ccagcctttc tgatcgcctc ggcagaaacg ttgggattgc ggataaatcg gtaagcgcct
     2581 tcctgcatgg cttcactacc ctctgatgag atggttattg atttaccaga atattttgcc
     2641 aattgggcgg cgacgttaac caagcgggca gtacggcgag gatcacccag cgccgccgaa
     2701 gagaacacag atttagccca gtcggccgca cgatgaagag cagaagttat catgaacgtt
     2761 accatgttag gaggtcacat ggaagatcag atcctggaaa acgggaaagg ttccgttcag
     2821 gacgctactt gtgtacggga tcggtcgacg gatcccaagc ttcttctaga ctgtctcttg
     2881 atcagatctg gccgcctagg ccgaattcga gctccttccc aaccttacca gagggcgccc
     2941 cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg
     3001 taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag
     3061 cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgt
     3121 tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa gctcgaggta
     3181 tgggattcac ctttatgttg ataagaaata aaagaaaatg ccaataggat atcggcattt
     3241 tcttttgcgt ttttatttgt taactgttaa ttgtccttgt tcaaggatgc tgtctttgac
     3301 aacagatgtt ttcttgcctt tgatgttcag caggaagctt ggcgcaaacg ttgattgttt
     3361 gtctgcgtag aatcctctgt ttgtcatata gcttgtaatc acgacattgt ttcctttcgc
     3421 ttgaggtaca gcgaagtgtg agtaagtaaa ggttacatcg ttaggatcaa gatccatttt
     3481 taacacaagg ccagttttgt tcagcggctt gtatgggcca gttaaagaat tagaaacata
     3541 accaagcatg taaatatcgt tagacgtaat gccgtcaatc gtcatttttg atccgcggga
     3601 gtcagtgaac aggtaccatt tgccgttcat tttaaagacg ttcgcgcgtt caatttcatc
     3661 tgttactgtg ttagatgcaa tcagcggttt catcactttt ttcagtgtgt aatcatcgtt
     3721 tagctcaatc ataccgagag cgccgtttgc taactcagcc gtgcgttttt tatcgctttg
     3781 cagaagtttt tgactttctt gacggaagaa tgatgtgctt ttgccatagt atgctttgtt
     3841 aaataaagat tcttcgcctt ggtagccatc ttcagttcca gtgtttgctt caaatactaa
     3901 gtatttgtgg cctttatctt ctacgtagtg aggatctctc agcgtatggt tgtcgcctga
     3961 gctgtagttg ccttcatcga tgaactgctg tacattttga tacgtttttc cgtcaccgtc
     4021 aaagattgat ttataatcct ctacaccgtt gatgttcaaa gagctgtctg atgctgatac
     4081 gttaacttgt gcagttgtca gtgtttgttt gccgtaatgt ttaccggaga aatcagtgta
     4141 gaataaacgg atttttccgt cagatgtaaa tgtggctgaa cctgaccatt cttgtgtttg
     4201 gtcttttagg atagaatcat ttgcatcgaa tttgtcgctg tctttaaaga cgcggccagc
     4261 gtttttccag ctgtcaatag aagtttcgcc gactttttga tagaacatgt aaatcgatgt
     4321 gtcatccgca tttttaggat ctccggctaa tgcaaagacg atgtggtagc cgtgatagtt
     4381 tgcgacagtg ccgtcagcgt tttgtaatgg ccagctgtcc caaacgtcca ggccttttgc
     4441 agaagagata tttttaattg tggacgaatc gaattcagga acttgatatt tttcattttt
     4501 ttgctgttca gggatttgca gcatatcatg gcgtgtaata tgggaaatgc cgtatgtttc
     4561 cttatatggc ttttggttcg tttctttcgc aaacgcttga gttgcgcctc ctgccagcag
     4621 tgcggtagta aaggttaata ctgttgcttg ttttgcaaac tttttgatgt tcatcgttca
     4681 tgtctccttt tttatgtact gtgttagcgg tctgcttctt ccagccctcc tgtttgaaga
     4741 tggcaagtta gttacgcaca ataaaaaaag acctaaaata tgtaaggggt gacgccaaag
     4801 tatacacttt gccctttaca cattttaggt cttgcctgct ttatcagtaa caaacccgcg
     4861 cgatttactt ttcgacctca ttctattaga ctctcgtttg gattgcaact ggtctatttt
     4921 cctcttttgt ttgatagaaa atcataaaag gatttgcaga ctacgggcct aaagaactag
     4981 agctcggtac ccggggatcc ggctgtaatc cgggcagcgc aacggaacat tcatcagtgt
     5041 aaaaatggaa tcaataaagc cctgcgcagc gcgcagggtc agcctgaata cgcgtttaat
     5101 gaccagcaca gtcgtgatgg caaggtcaga atagcgctga ggtctgcctc gtgaagaagg
     5161 tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc
     5221 acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc
     5281 cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt
     5341 tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     5401 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     5461 tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat
     5521 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     5581 aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     5641 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     5701 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     5761 tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     5821 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     5881 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     5941 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     6001 gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt
     6061 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     6121 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     6181 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     6241 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     6301 gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg aataaatcga
     6361 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
     6421 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
     6481 ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca
     6541 ccttcttcac gaggcagacc tcagcgctat tctgaccttg ccatcacgac tgtgctggtc
     6601 attaaacgcg tattcaggct gaccctgcgc gctgcgcagg gctttattga ttccattttt
     6661 acactgatga atgttccgtt gcgctgcccg gattacagcc ggatcctcta gagtcgacct
     6721 gcaggcatgc gacaacagat gttttcttgc ctttgatgtt cagcaggaag cttggcgcaa
     6781 acgttgattg tttgtctgcg tagaatcctc tgtttgtcat atagcttgta atcacgacat
     6841 tgtttccttt cgcttgaggt acagcgaagt gtgagtaagt aaaggttaca tcgttaggat
     6901 caagatccat ttttaacaca aggccagttt tgttcagcgg cttgtatggg ccagttaaag
     6961 aattagaaac ataaccaagc atgtaaatat cgttagacgt aatgccgtca atcgtcattt
     7021 ttgatccgcg ggagtcagtg aacaggtacc atttgccgtt cattttaaag acgttcgcgc
     7081 gttcaatttc atctgttact gtgttagatg caatcagcgg tttcatcact tttttcagtg
     7141 tgtaatcatc gtttagctca atcataccga gggcccatgg catatgtccg gagcggccgc
     7201 acttgtgtat aagagtcaga attccacaaa ttgttatccg ctcacaattc cacatgtgga
     7261 attccacatg tggaattccc atgtcagccg ttaagtgttc ctgtgtcact caaaattgct
     7321 ttgagaggct ctaagggctt ctcagtgcgt tacatccctg gcttgttgtc cacaaccgtt
     7381 aaaccttaaa agctttaaaa gccttatata ttcttttttt tcttataaaa cttaaaacct
     7441 tagaggctat ttaagttgct gatttatatt aattttattg ttcaaacatg agagcttagt
     7501 acgtgaaaca tgagagctta gtacgttagc catgagagct tagtacgtta gccatgaggg
     7561 tttagttcgt taaacatgag agcttagtac gttaaacatg agagcttagt acgtgaaaca
     7621 tgagagctta gtacgtacta tcaacaggtt gaactgctga tcttcagatc ctctacgccg
     7681 gacgcatcgt ggccggatcc agccgaccag gctttccacg cccgcgtgcc gctccatgtc
     7741 gttcgcgcgg ttctcggaaa cgcgctgccg cgtttcgtga ttgtcacgct caagcccgta
     7801 gtcccgttcg agcgtcgcgc agaggtcagc gagggcgcgg taggcccgat acggctcatg
     7861 gatggtgttt cgggtcgggt gaatcttgtt gatggcgata tggatgtgca ggttgtcggt
     7921 gtcgtgatgc acggcactga cgcgctgatg ctcggcgaag ccaagcccag cgcagatgcg
     7981 gtcctcaatc gcgcgcaacg tctccgcgtc gggcttctct cccgcgcgga agctaaccag
     8041 caggtgatag gtcttgtcgg cctcggaacg ggtgttgccg tgctgggtcg ccatcacctc
     8101 ggccatgaca gcgggcaggg tgtttgcctc gcagttcgtg acgcgcacgt gacccaggcg
     8161 ctcggtcttg ccttgctcgt cggtgatgta cttcaccagc tccgcgaagt cgctcttctt
     8221 gatggagcgc atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct
     8281 aaaaaagtca tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt
     8341 cctgcttctc ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg
     8401 tgcgcgggtc gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat
     8461 tgatgcgggc cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct
     8521 ggccgacggc cagcaggtag gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa
     8581 tcgctcttcg ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg
     8641 gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc
     8701 ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg
     8761 aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac
     8821 accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg
     8881 gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgct
     8941 gcttccctgc tgttttgtgg aatatctacc gactggaaac aggcaaatgc aggaaattac
     9001 tgaactgagg ggacaggcga gagacgatgc caaagagcta caccgacgag ctggccgagt
     9061 gggttgaatc ccgcgcggcc aagaagcgcc ggcgtgatga ggctgcggtt gcgttcctgg
     9121 cggtgagggc ggatgtcgag gcggcgttag cgtccggcta tgcgctcgtc accatttggg
     9181 agcacatgcg ggaaacgggg aaggtcaagt tctcctacga gacgttccgc tcgcacgcca
     9241 ggcggcacat caaggccaag cccgccgatg tgcccgcacc gcaggccaag gctgcggaac
     9301 ccgcgccggc acccaagacg ccggagccac ggcggccgaa gcaggggggc aaggctgaaa
     9361 agccggcccc cgctgcggcc ccgaccggct tcaccttcaa cccaacaccg gacaaaaagg
     9421 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     9481 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     9541 gcttgtttcg gcgtgggtat ggtggcag
//
