LOCUS       62056_22445        9374 bp DNA     circular SYN 06-APR-2019
DEFINITION  Transformation vector pUTSW, complete sequence.
ACCESSION   MK424951
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9374)
  AUTHORS   Luo J, Shen P, Chen J.
  TITLE     A modular toolset of phiC31-based fluorescent protein tagging 
            vectors for Drosophila
  JOURNAL   Fly (Austin) (2019) In press
  PUBMED    30885036
REFERENCE   2  (bases 1 to 9374)
  AUTHORS   Luo J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2019) Model Animal Research Center, Nanjing 
            University, 12 Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China
REFERENCE   3  (bases 1 to 9374)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JAN-2019) Model Animal Research Center, Nanjing University, 12 
            Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China"
FEATURES             Location/Qualifiers
     source          1..9374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4809..4842
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4843..5273
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     protein_bind    5309..5403
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5422..5660
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     intron          5797..5862
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5992..6012
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    complement(6043..6285)
                     /label=hsp70 basal promoter
                     /note="hsp70 basal promoter"
     polyA_signal    6284..6418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6433..6818
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     protein_bind    6898..6967
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(7186..7204)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7225..7241)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7249..7265)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7273..7303)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7318..7339)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7627..8215)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8389..9246)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9247..9351)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagtat aacttcgtat aatgtatgct atacgaagtt attggccacg taataagtgt
     4861 gcgttgaatt tattcgcaaa aacattgcat attttcggca aagtaaaatt ttgttgcata
     4921 ccttatcaaa aaataagtgc tgcatacttt ttagagaaac caaataattt tttattgcat
     4981 acccgttttt aataaaatac attgcatacc ctcttttaat aaaaaatatt gcatactttg
     5041 acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata cccgttttta
     5101 ataaaataca ttgcataccc tcttttaata aaraatattg catacgttga cgaaacaaat
     5161 tttcgttgca tacccaataa aagattatta tattgcatac cttttcttgc cataccattt
     5221 agccgatcaa ttgtgctcgg caacagtata tttgtggtgt gccaaccaac aacacgcgta
     5281 ctggatccaa gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg agtactgtcc
     5341 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
     5401 gagcggagac tctagcgagc gccggagtat aaatagaggc gcttcgtcta cggagcgaca
     5461 attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag
     5521 cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa
     5581 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
     5641 aatacaagaa gagaactctg aatagggaat tgggaattcg ttaacagatc tgcggccgcg
     5701 gctcgagggt acctctagag gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     5761 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     5821 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     5881 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     5941 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     6001 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     6061 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
     6121 gcactgctat acaagaaaat tatggaaaaa tatttgatgt atagtgcctt gactagagat
     6181 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     6241 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     6301 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6361 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcct
     6421 caaggccttg gccacgtaat aagtgtgcgt tgaatttatt cgcaaaaaca ttgcatattt
     6481 tcggcaaagt aaaattttgt tgcatacctt atcaaaaaat aagtgctgca tactttttag
     6541 agaaaccaaa taatttttta ttgcataccc gtttttaata aaatacattg cataccctct
     6601 tttaataaaa aatattgcat actttgacga aacaaatttt cgttgcatac ccaataaaag
     6661 attattatat tgcatacccg tttttaataa aatacattgc ataccctctt ttaataaara
     6721 atattgcata cgttgacgaa acaaattttc gttgcatacc caataaaaga ttattatatt
     6781 gcataccttt tcttgccata ccatttagcc gatcaattgt gctcggcaac agtatatttg
     6841 tggtgtgcca accaacaaca ctagagatcc actagtgtcg acgatgtagg tcacggtctc
     6901 gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc
     6961 acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc
     7021 gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca cggtgagcac
     7081 gggacgtgcg acggcgtcgg cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac
     7141 ggtcacggcg ggcatgtcga cactagttct agccagcttt tgttcccttt agtgagggtt
     7201 aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7261 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7321 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7381 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7441 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7501 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7561 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7621 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7681 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7741 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7801 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7861 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7921 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7981 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8041 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8101 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8161 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8221 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8281 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8341 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8401 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8461 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8521 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8581 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8641 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8701 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8761 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8821 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8881 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8941 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9001 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9061 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9121 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9181 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9241 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9301 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9361 ccgaaaagtg ccac
//
