LOCUS       62056_22440        7118 bp DNA     circular SYN 06-APR-2019
DEFINITION  Transformation vector pUTSV, complete sequence.
ACCESSION   MK424961
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Luo J, Shen P, Chen J.
  TITLE     A modular toolset of phiC31-based fluorescent protein tagging 
            vectors for Drosophila
  JOURNAL   Fly (Austin) (2019) In press
  PUBMED    30885036
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Luo J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2019) Model Animal Research Center, Nanjing 
            University, 12 Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China
REFERENCE   3  (bases 1 to 7118)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JAN-2019) Model Animal Research Center, Nanjing University, 12 
            Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China"
FEATURES             Location/Qualifiers
     source          1..7118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    667..2546
                     /label=vermilion
                     /note="vermilion"
     protein_bind    2553..2586
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2587..3017
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     polyA_signal    complement(3028..3162)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3434..3454)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(3584..3649)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     misc_feature    complement(3727..3781)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(3786..4024)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    complement(4043..4137)
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_feature    4173..4603
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    4642..4711
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(4930..4948)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4969..4985)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4993..5009)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5017..5047)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5062..5083)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5371..5959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6133..6990)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6991..7095)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggccccattt gtgtgcgccg cactttcacc tcaagtgatt gataattccc agcctatctg
      721 gcagtgccca tcgcccagat caccgactgt gcaatcagtc ggaactggag ctctctcgct
      781 ctgttatcgg ttcgctgggg tctcatctcc ggtccgctgg cggagatcag ttcgccagca
      841 tccgccgctc gaggagtcac gatctgatct gagctgtgca ccatgagctg tccctatgca
      901 ggaaacgggt gagcaccagc acgtgctgtc caggaatgcc aatcgatctt cagttctgcg
      961 attcaattca aacccataca gaaacgatca cgatgattcg gcggtgccat taaccacgga
     1021 agtgggcaaa atctatggag agtatctgat gctggacaaa ctgctggatg cccagtgtat
     1081 gctgtccgag gaggacaagc gacccgtgca cgatgagcat ctgttcatca tcacgcacca
     1141 gggtgagtag gtttacaact ttgatgacaa cactcaatgg catttaagta ccttcgccac
     1201 agcctacgag ctttggttca agcagatcat ctttgagttc gactccatac gagacatgtt
     1261 ggatgcagag gtcatcgatg aaaccaagac gctggagatt gtcaagcgac tgaaccgagt
     1321 ggttctgatt ctaaaagtga gtgctttctg aatctcttac caaaatccgt ttataacttc
     1381 ctttgtacag ctcctggtgg accaagtgcc cattctggag accatgaccc cgctagactt
     1441 catggacttc cgcaagtacc tggcacccgc atctggtttt cagtcgctgc agttccgttt
     1501 gatcgagaac aagctgggag ttctgacaga gcagcgggtg agatacaacc agaagtactc
     1561 ggatgtcttt agcgacgagg aggcgcggaa ctcgattcgc aactcggaga aagatccctc
     1621 gctactggag ctagtgcagc gatggctgga gaggacgccc ggactggagg agagtggctt
     1681 caacttctgg gccaagtttc aggagagcgt cgatcgattc ctggaggcgc aggtacagag
     1741 cgccatggag gagcccgtgg agaaggcgaa aaactaccgc ctcatggaca ttgagaagcg
     1801 acgcgaggtg tatcgctcca tctttgatcc ggcagtgcac gatgcactgg tgcgtcgtgg
     1861 ggatcgccgg tttagccatc gtgcccttca gggagccatc atgatcacct tctataggga
     1921 tgaacccagg ttcagccaac cacaccagtt gctcaccctg ctcatggaca tcgactcgtt
     1981 aataaccaag tggagatgta agtattgcat tctttgatac tcttttataa atatatctta
     2041 tgtttaagac tggttttcct aaccaaatac tttctattcc cgccgcagac aatcacgtga
     2101 tcatggtgca acgcatgatt ggatcccaac agttgggcac tggtggctcg tctggatatc
     2161 aatatctgcg ctccactctc aggtgatcat cgcagatgtg attatatcgg ggatcaatga
     2221 actcaaactg ttctcccttt gttttttttt ggtttcagtg atcggtacaa ggtgtttctg
     2281 gatctgttca atctgtccac ttttctgatt ccccgcgagg cgattccacc gctggacgag
     2341 accattcgca agaaactgat caacaaaagt gtctgacaat cggcagggta tccaattcgt
     2401 caatgtttgg ctatgcgttg tttgttctgc ctactgtttt gtcgttttgg tgtaataaaa
     2461 ttacttgttt agtctttgtt atcacatttg atgtgttcct tttctttatg tctgacatat
     2521 aatacatata acataacaaa ataaatgcta gtataacttc gtataatgta tgctatacga
     2581 agttattggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     2641 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     2701 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     2761 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     2821 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaagaat
     2881 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     2941 ataccttttc ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg
     3001 gtgtgccaac caacaacacg cgtactggat ccagacatga taagatacat tgatgagttt
     3061 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     3121 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     3181 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     3241 tacaaatgtg gtatggctga ttatgatctc tagtcaaggc actatacatc aaatattttt
     3301 ccataatttt cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag
     3361 caagagttct attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt
     3421 ccttggggtc ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag
     3481 tagcctcatc atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg
     3541 cattccacca ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa
     3601 ttagaatcag tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt
     3661 aaatctctgt aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa
     3721 agatcctcta gaggtaccct cgagccgcgg ccgcagatct gttaacgaat tcccaattcc
     3781 ctattcagag ttctcttctt gtattcaata attacttctt ggcagatttc agtagttgca
     3841 gttgatttac ttggttgctg gttactttta attgattcac tttaacttgc actttactgc
     3901 agattgttta gcttgttcag ctgcgcttgt ttatttgctt agctttcgct tagcgacgtg
     3961 ttcactttgc ttgtttgaat tgaattgtcg ctccgtagac gaagcgcctc tatttatact
     4021 ccggcgctcg ctagagtctc cgctcggagg acagtactcc gctcggagga cagtactccg
     4081 ctcggaggac agtactccgc tcggaggaca gtactccgct cggaggacag tactccgacc
     4141 tgcaggcatg caagcttgga tcctcaaggc cttggccacg taataagtgt gcgttgaatt
     4201 tattcgcaaa aacattgcat attttcggca aagtaaaatt ttgttgcata ccttatcaaa
     4261 aaataagtgc tgcatacttt ttagagaaac caaataattt tttattgcat acccgttttt
     4321 aataaaatac attgcatacc ctcttttaat aaaaaatatt gcatactttg acgaaacaaa
     4381 ttttcgttgc atacccaata aaagattatt atattgcata cccgttttta ataaaataca
     4441 ttgcataccc tcttttaata aagaatattg catacgttga cgaaacaaat tttcgttgca
     4501 tacccaataa aagattatta tattgcatac cttttcttgc cataccattt agccgatcaa
     4561 ttgtgctcgg caacagtata tttgtggtgt gccaaccaac aacactagag atccactagt
     4621 gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg tgccagggcg tgcccttggg
     4681 ctccccgggc gcgtactcca cctcacccat ctggtccatc atgatgaacg ggtcgaggtg
     4741 gcggtagttg atcccggcga acgcgcggcg caccgggaag ccctcgccct cgaaaccgct
     4801 gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg tcggcgggtg cggatacgcg
     4861 gggcagcgtc agcgggttct cgacggtcac ggcgggcatg tcgacactag ttctagccag
     4921 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     4981 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5041 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5101 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5161 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5221 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5281 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5341 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5401 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5461 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5521 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5581 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5641 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5701 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5761 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     5821 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5881 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5941 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6001 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6061 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6121 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6181 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6241 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6301 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6361 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6421 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6481 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6541 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6601 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6661 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6721 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6781 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6841 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6901 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6961 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7021 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7081 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
