LOCUS       40924_45803        8296 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transposon delivery vector pUT-miniTn5-gfp-tet, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Schuler L, Wattiau P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2003) Bioengineering Unit, UCL, Place Croix du Sud
            2 Pbox 19, Louvain-la-Neuve 1348, Belgium
REFERENCE   2  (bases 1 to 8296)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8296)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2003) Bioengineering Unit, UCL, Place Croix du Sud 2 Pbox 
            19, Louvain-la-Neuve 1348, Belgium"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(314..1171)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1172..1276)
                     /label=AmpR promoter
     CDS             complement(1326..2753)
                     /codon_start=1
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     misc_feature    2871..2889
                     /label=Tn5 IE end
                     /note="Tn5 IE end"
     CDS             complement(3359..4546)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     5107..5123
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5157..5870)
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    5929..5947
                     /label=Tn5 OE end
                     /note="Tn5 OE end"
     protein_bind    complement(5959..5975)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(6010..6398)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(7019..7387)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(7420..7529)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gccccgtggc cgggggactg ttgggcgcca tctccttgct gcctcgcgcg tttcggtgat
       61 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      181 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
      241 cagagcagat tgtactgaga gtgcaccata aatcaatcta aagtatatat gagtaaactt
      301 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
      361 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
      421 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
      481 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
      541 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
      601 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
      661 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
      721 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
      781 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
      841 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
      901 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
      961 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1021 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1081 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     1141 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     1201 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     1261 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcagatctg caggtcgacc
     1321 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
     1381 ctttgcaggg cttcccaacc ttcccagagg gcgccccagc tggcaattcc ggttcgcttg
     1441 ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct
     1501 ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg
     1561 gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc
     1621 gccctgagtg cttgcggcag cgtgaagctt tctctgagct gtaacagcct gaccgcaaca
     1681 aacgagagga tcgagaccat ccgctccaga ttatccggct cctccatgcg ttgcctctcg
     1741 gctcctgctc cggttttcca tgccttatgg aactcctcga tccgccagcg atgggtataa
     1801 atgtcgatga cgcgcaaggc ttgggctagc gactcgaccg gttcgctggt cagcaacaac
     1861 catttcaacg gggtctcacc cttgggcggg ttaatctcct cggccagcac cgcgttgagc
     1921 gtgatattcc cctgttttag cgtgatgcgc ccactgcgca ggctcaagct cgccttgcgg
     1981 gctggtcgat ttttacgttt accgcgttta tccaccacgc ccttttgcgg aatgctgatc
     2041 tgatagccac ccaactccgg ttggttcttc agatggtcgt acagatacaa cccagactct
     2101 acgtccttgc gtgggtgctt ggagcgcacc acgaagcgct cgttatgcgc cagtttgtcc
     2161 tgcagataag catgaatatc ggcttcgcgg tcacagaccg caatcacgtt gctcatcatg
     2221 ctgcccatgc gtaaccggct agttgcggcg gctgccagcc atttgccact ctccttttca
     2281 tccgcatcgg cagggtcatc cgggcgcatc caccactcct gatgcagtaa tcctacggtg
     2341 cggaatgtgg tggcctcgag caagagaacg gagtgaaccc accatccgcg ggatttatcc
     2401 tgaatagagc ccagcttgcc aagctcttcg gcgacctggt ggcgataact caaagaggtg
     2461 gtgtcctcaa tggccagcag ttcgggaaac tcctgagcca acttgactgt ttgcatggcg
     2521 ccagcctttc tgatcgcctc ggcagaaacg ttgggattgc ggataaatcg gtaagcgcct
     2581 tcctgcatgg cttcactacc ctctgatgag atggttattg atttaccaga atattttgcc
     2641 aattgggcgg cgacgttaac caagcgggca gtacggcgag gatcacccag cgccgccgaa
     2701 gagaacacag atttagccca gtcggccgca cgatgaagag cagaagttat catgaacgtt
     2761 accatgttag gaggtcacat ggaagatcag atcctggaaa acgggaaagg ttccgttcag
     2821 gacgctactt gtgtacggga tcggtcgacg gatcccaagc ttcttctaga ctgtctcttg
     2881 atcagatctg gccgcctagg ccgaattccc ggggatccgg tgattgattg agcaagcttt
     2941 atgcttgtaa accgttttgt gaaaaaattt ttaaaataaa aaaggggacc tctagggtcc
     3001 ccaattaatt agtaatataa tctattaaag gtcattcaaa aggtcatcca ccggatcaat
     3061 tcccctgctc gcgcaggctg ggtgccaagc tctcgggtaa catcaaggcc cgatccttgg
     3121 agcccttgcc ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc ttgacccgca
     3181 gttgcaaacc ctcactgatc cgccgagatg cgccgcgtgc ggctgctgga gatggcggac
     3241 gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga
     3301 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg
     3361 tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg
     3421 cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg
     3481 tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa
     3541 gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc
     3601 cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga
     3661 acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct
     3721 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc
     3781 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga
     3841 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca
     3901 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
     3961 ctctcaaggg catcggtcgg cgctctccct tatgcgactc ctgcattagg aagcagccca
     4021 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgt aaggagatgg
     4081 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     4141 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     4201 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatccaca
     4261 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca
     4321 ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt
     4381 gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga
     4441 cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
     4501 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
     4561 tgcgttagca atttaactgt gataaactac cgcattaaag ctagatcagt tggaagaatt
     4621 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg
     4681 ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat
     4741 aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa
     4801 taagccctac acaaattggg agatatatca tgaaaggctg gctttttctt gttatcgcaa
     4861 tagttggcga agtaatcgca acatccgcat taaaatctag cgagggcttt actaagctga
     4921 tccggtggat gaccttttga atgaccttta atagattata ttactaatta attggggacc
     4981 ctagaggtcc ccttttttat tttaaaaatt ttttcacaaa acggtttaca agcataaagc
     5041 ttgctcaatc aatcaccgga tccccgggaa ttcgtcgaca agcttcggcc gcctaggccg
     5101 cggcctcgag gtcgacggta tcgataagct tgcatgcctg caggtctgga catttatttg
     5161 tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag aaggaccatg
     5221 tggtctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag gtaatggttg
     5281 tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg gtctgctagt
     5341 tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat tccattcttt
     5401 tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa tttgtgtcca
     5461 agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt aacaagggta
     5521 tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt gaaaaatata
     5581 gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg ccgtttcata
     5641 tgatctgggt atctcgcaaa gcattgaaga ccatacgcga aagtagtgac aagtgttggc
     5701 catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt tccgtatgtt
     5761 gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc accatctaat
     5821 tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttactcat atgtatatct
     5881 ccttcttaaa tctagaggat ccccgggtac cgagctcgaa ttggccgcac ttgtgtataa
     5941 gagtcagaat tccacaaatt gttatccgct cacaattcca catgtggaat tccacatgtg
     6001 gaattcccat gtcagccgtt aagtgttcct gtgtcactca aaattgcttt gagaggctct
     6061 aagggcttct cagtgcgtta catccctggc ttgttgtcca caaccgttaa accttaaaag
     6121 ctttaaaagc cttatatatt cttttttttc ttataaaact taaaacctta gaggctattt
     6181 aagttgctga tttatattaa ttttattgtt caaacatgag agcttagtac gtgaaacatg
     6241 agagcttagt acgttagcca tgagagctta gtacgttagc catgagggtt tagttcgtta
     6301 aacatgagag cttagtacgt taaacatgag agcttagtac gtgaaacatg agagcttagt
     6361 acgtactatc aacaggttga actgctgatc ttcagatcct ctacgccgga cgcatcgtgg
     6421 ccggatccag ccgaccaggc tttccacgcc cgcgtgccgc tccatgtcgt tcgcgcggtt
     6481 ctcggaaacg cgctgccgcg tttcgtgatt gtcacgctca agcccgtagt cccgttcgag
     6541 cgtcgcgcag aggtcagcga gggcgcggta ggcccgatac ggctcatgga tggtgtttcg
     6601 ggtcgggtga atcttgttga tggcgatatg gatgtgcagg ttgtcggtgt cgtgatgcac
     6661 ggcactgacg cgctgatgct cggcgaagcc aagcccagcg cagatgcggt cctcaatcgc
     6721 gcgcaacgtc tccgcgtcgg gcttctctcc cgcgcggaag ctaaccagca ggtgataggt
     6781 cttgtcggcc tcggaacggg tgttgccgtg ctgggtcgcc atcacctcgg ccatgacagc
     6841 gggcagggtg tttgcctcgc agttcgtgac gcgcacgtga cccaggcgct cggtcttgcc
     6901 ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga tggagcgcat
     6961 ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg
     7021 gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt
     7081 cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt
     7141 cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca
     7201 gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca
     7261 gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt
     7321 cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat
     7381 cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag
     7441 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
     7501 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
     7561 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa
     7621 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagcgctgc ttccctgctg
     7681 ttttgtggaa tatctaccga ctggaaacag gcaaatgcag gaaattactg aactgagggg
     7741 acaggcgaga gacgatgcca aagagctaca ccgacgagct ggccgagtgg gttgaatccc
     7801 gcgcggccaa gaagcgccgg cgtgatgagg ctgcggttgc gttcctggcg gtgagggcgg
     7861 atgtcgaggc ggcgttagcg tccggctatg cgctcgtcac catttgggag cacatgcggg
     7921 aaacggggaa ggtcaagttc tcctacgaga cgttccgctc gcacgccagg cggcacatca
     7981 aggccaagcc cgccgatgtg cccgcaccgc aggccaaggc tgcggaaccc gcgccggcac
     8041 ccaagacgcc ggagccacgg cggccgaagc aggggggcaa ggctgaaaag ccggcccccg
     8101 ctgcggcccc gaccggcttc accttcaacc caacaccgga caaaaaggat cctctacgcc
     8161 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     8221 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     8281 gtgggtatgg tggcag
//
