LOCUS       62056_22435       11715 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            843..952
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             985..1353
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      1974..2362
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2477..3556)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(3557..3634)
                     /label=lacI promoter
     promoter        3680..3708
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             3755..4462
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      4499..4546
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5221..5751)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(5940..5968)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        6849..6867
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6868..6892
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             6936..8363
                     /codon_start=1
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     CDS             complement(8469..9887)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(9888..10333)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     promoter        10515..10619
                     /label=AmpR promoter
     CDS             10620..11477
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gaggttttca ccgtcatcac cgaaacgcgc gaggcagcaa ggagatggcg cccaacagtc
       61 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
      121 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
      181 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatccttttt gtccggtgtt
      241 gggttgaagg tgaagccggt cggggccgca gcgggggccg gcttttcagc cttgcccccc
      301 tgcttcggcc gccgtggctc cggcgtcttg ggtgccggcg cgggttccgc agccttggcc
      361 tgcggtgcgg gcacatcggc gggcttggcc ttgatgtgcc gcctggcgtg cgagcggaac
      421 gtctcgtagg agaacttgac cttccccgtt tcccgcatgt gctcccaaat ggtgacgagc
      481 gcatagccgg acgctaacgc cgcctcgaca tccgccctca ccgccaggaa cgcaaccgca
      541 gcctcatcac gccggcgctt cttggccgcg cgggattcaa cccactcggc cagctcgtcg
      601 gtgtagctct ttggcatcgt ctctcgcctg tcccctcagt tcagtaattt cctgcatttg
      661 cctgtttcca gtcggtagat attccacaaa acagcaggga agcagcgctt ttccgctgca
      721 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga
      781 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag
      841 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct
      901 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc
      961 ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc
     1021 ccacctatca aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg
     1081 gcggccggca tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg
     1141 ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc
     1201 ctgggcggcc tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat
     1261 gccacgatcc tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc
     1321 atgatgggcg tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg
     1381 gggggtgcgc gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc
     1441 ggagctggtg aagtacatca ccgacgagca aggcaagacc gagcgcctgg gtcacgtgcg
     1501 cgtcacgaac tgcgaggcaa acaccctgcc cgctgtcatg gccgaggtga tggcgaccca
     1561 gcacggcaac acccgttccg aggccgacaa gacctatcac ctgctggtta gcttccgcgc
     1621 gggagagaag cccgacgcgg agacgttgcg cgcgattgag gaccgcatct gcgctgggct
     1681 tggcttcgcc gagcatcagc gcgtcagtgc cgtgcatcac gacaccgaca acctgcacat
     1741 ccatatcgcc atcaacaaga ttcacccgac ccgaaacacc atccatgagc cgtatcgggc
     1801 ctaccgcgcc ctcgctgacc tctgcgcgac gctcgaacgg gactacgggc ttgagcgtga
     1861 caatcacgaa acgcggcagc gcgtttccga gaaccgcgcg aacgacatgg agcggcacgc
     1921 gggcgtggaa agcctggtcg gctggatccg gccacgatgc gtccggcgta gaggatctga
     1981 agatcagcag ttcaacctgt tgatagtacg tactaagctc tcatgtttca cgtactaagc
     2041 tctcatgttt aacgtactaa gctctcatgt ttaacgaact aaaccctcat ggctaacgta
     2101 ctaagctctc atggctaacg tactaagctc tcatgtttca cgtactaagc tctcatgttt
     2161 gaacaataaa attaatataa atcagcaact taaatagcct ctaaggtttt aagttttata
     2221 agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg tttaacggtt gtggacaaca
     2281 agccagggat gtaacgcact gagaagccct tagagcctct caaagcaatt ttgagtgaca
     2341 caggaacact taacggctga catgggaatt ccacatgtgg aattccacat gtggaattgt
     2401 gagcggataa caatttgtgg aattcccggg agagctcctg actcttatac acaagttcgg
     2461 ccacggccta ggctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2521 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc
     2581 ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt
     2641 gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta
     2701 acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatatccg
     2761 caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt
     2821 tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa
     2881 aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag
     2941 tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg
     3001 ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac
     3061 cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata
     3121 acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat
     3181 agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg
     3241 cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc
     3301 gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa
     3361 cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat
     3421 tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct
     3481 ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata
     3541 acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca
     3601 taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc
     3661 gactcctgca ttaggaaatt tgacaattaa tcatcggctc gtataatgtg tggaatgtct
     3721 catgagcgga tatttcacac aggaaacagt attcatggtg agcaagggcg aggaggataa
     3781 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     3841 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     3901 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     3961 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     4021 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     4081 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     4141 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     4201 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     4261 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     4321 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     4381 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     4441 cggcatggac gagctgtaca agtaattaac ctaggctgct gccaccgctg agcaataact
     4501 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttggcgg ccgcagcttg
     4561 tcgacgaatt cccggggatc cggtgattga ttgagcaagc tttatgcttg taaaccgttt
     4621 tgtgaaaaaa tttttaaaat aaaaaagggg acctctaggg tccccaatta attagtaata
     4681 taatctatta aaggtcattc aaaaggtcat ccaccggatc aattcccctg ctcacacaga
     4741 ctgggtacca agctctcggt aacatcaagg cccgatcctt ggagcccttg ccctcccgac
     4801 gatgatcgtg ccgtgatcga aatccagatc cttgacccac agttgcaaac cctcactgat
     4861 ccgtcccgtc cgggtcgacc aaagcggcca tcgtgcctcc ccactcctgc agttcggggg
     4921 catggatgcg cggatagccg ctgctggttt cctggatgcc gacggatttg cactgccggt
     4981 agaactccgc gaggtcgtcc agcctcaggc agcagctgaa ccaactcgcg aggggatcga
     5041 gcccggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg
     5101 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat
     5161 ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctta
     5221 ggtggcggta cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt
     5281 atagagagcc actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc
     5341 aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg
     5401 gtgctcgccg gagactgcga gatcatagat atagatctca ctacgcggct gctcaaacct
     5461 gggcagaacg taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc
     5521 gatgaatgtc ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga
     5581 gtaggtggct acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt
     5641 gacttggtca gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt
     5701 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc
     5761 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
     5821 tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac
     5881 cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca
     5941 gtttacgaac cgaacaggct tatgtcaagc gaaacgatcc tcatcctgtc tcttgatcag
     6001 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     6061 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     6121 ataaaaccgc ccagtctagc tatcgtcatg taagcccact gcaagctacc tctttctctt
     6181 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtagcac
     6241 cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgat
     6301 gcttgcggca gcgtgaagct cgcgcagatc agttggttga ttttgtccac tacgtgaaag
     6361 gcgaatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt
     6421 cgcggcgcgg ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact
     6481 atcaggtcaa gtcgctttta ttatttttaa gcgtgcataa taagccctac acaaaattgg
     6541 gagatatatc atgaaaggcg cttttcttgt tatcgcaata gttgggcgaa gtaatcgcaa
     6601 catccgcatt aacaatctag cgagggcttg gtaaggtgat ccggtggatg accttttgaa
     6661 tgacctttaa tagattatat tactaattaa ttggggaccc tagaggtccc cttttttatt
     6721 ttaaaaattt tttcacaaaa cggtttacaa gcataaagct tgctcaatca atcaccggat
     6781 ccccgggaat tcggcctagg tggccagatc tgatcaagag acagaaacgc aaaagaaaat
     6841 gccgatatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
     6901 aataattttg ttaactttaa gaaggagata tacatatgat taccagtgca ctgcatcgtg
     6961 cggcggattg ggcgaaaagc gtgttttcta gtgctgcgct gggtgatccg cgtcgtaccg
     7021 cgcgtctggt gaatgttgcg gcgcaactgg ccaaatatag cggcaaaagc attaccatta
     7081 gcagcgaagg cagcaaagcc atgcaggaag gcgcgtatcg ttttattcgt aatccgaacg
     7141 tgagcgcgga agcgattcgt aaagcgggtg ccatgcagac cgtgaaactg gcccaggaat
     7201 ttccggaact gctggcaatt gaagatacca cctctctgag ctatcgtcat caggtggcgg
     7261 aagaactggg caaactgggt agcattcagg ataaaagccg tggttggtgg gtgcatagcg
     7321 tgctgctgct ggaagcgacc acctttcgta ccgtgggcct gctgcatcaa gaatggtgga
     7381 tgcgtccgga tgatccggcg gatgcggatg aaaaagaaag cggcaaatgg ctggccgctg
     7441 ctgcaacttc gcgtctgaga atgggcagca tgatgagcaa cgtgattgcg gtgtgcgatc
     7501 gtgaagcgga tattcatgcg tatctgcaag ataaactggc ccataacgaa cgttttgtgg
     7561 tgcgtagcaa acatccgcgt aaagatgtgg aaagcggcct gtatctgtat gatcacctga
     7621 aaaaccagcc ggaactgggc ggctatcaga ttagcattcc gcagaaaggc gtggtggata
     7681 aacgtggcaa acgtaaaaac cgtccggcgc gtaaagcgag cctgagcctg cgtagcggcc
     7741 gtattaccct gaaacagggc aacattaccc tgaacgcggt gctggccgaa gaaattaatc
     7801 cgccgaaagg cgaaaccccg ctgaaatggc tgctgctgac cagcgagccg gtggaaagtc
     7861 tggcccaagc gctgcgtgtg attgatattt atacccatcg ttggcgcatt gaagaatttc
     7921 acaaagcgtg gaaaacgggt gcgggtgcgg aacgtcagcg tatggaagaa ccggataacc
     7981 tggaacgtat ggtgagcatt ctgagctttg tggcggtgcg tctgctgcaa ctgcgtgaat
     8041 cttttactcc gccgcaagca ctgcgtgcgc agggcctgct gaaagaagcg gaacacgttg
     8101 aaagccagag cgcggaaacc gtgctgaccc cggatgaatg ccaactgctg ggctatctgg
     8161 ataaaggcaa acgcaaacgc aaagaaaaag cgggcagcct gcaatgggcg tatatggcga
     8221 ttgcgcgtct gggcggcttt atggatagca aacgtaccgg cattgcgagc tggggtgcgc
     8281 tgtgggaagg ttgggaagcg ctgcaaagca aactggatgg ctttctggcc gcgaaagacc
     8341 tgatggcgca gggcattaaa atctaactag cataacccct tggggcctct aaacgggtct
     8401 tgaggggttt tttgacgcgt gctatcgtca tgtaagccca ctgatcggca ttttcttttg
     8461 cgtttttatt tgttaactgt taattgtcct tgttcaagga tgctgtcttt gacaacagat
     8521 gttttcttgc ctttgatgtt cagcaggaag ctcggcgcaa acgttgattg tttgtctgcg
     8581 tagaatcctc tgtttgtcat atagcttgta atcacgacat tgtttccttt cgcttgaggt
     8641 acagcgaagt gtgagtaagt aaaggttaca tcgttaggat caagatccat ttttaacaca
     8701 aggccagttt tgttcagcgg cttgtatggg ccagttaaag aattagaaac ataaccaagc
     8761 atgtaaatat cgttagacgt aatgccgtca atcgtcattt ttgatccgcg ggagtcagtg
     8821 aacaggtacc atttgccgtt cattttaaag acgttcgcgc gttcaatttc atctgttact
     8881 gtgttagatg caatcagcgg tttcatcact tttttcagtg tgtaatcatc gtttagctca
     8941 atcataccga gagcgccgtt tgctaactca gccgtgcgtt ttttatcgct ttgcagaagt
     9001 ttttgacttt cttgacggaa gaatgatgtg cttttgccat agtatgcttt gttaaataaa
     9061 gattcttcgc cttggtagcc atcttcagtt ccagtgtttg cttcaaatac taagtatttg
     9121 tggcctttat cttctacgta gtgaggatct ctcagcgtat ggttgtcgcc tgagctgtag
     9181 ttgccttcat cgatgaactg ctgtacattt tgatacgttt ttccgtcacc gtcaaagatt
     9241 gatttataat cctctacacc gttgatgttc aaagagctgt ctgatgctga tacgttaact
     9301 tgtgcagttg tcagtgtttg tttgccgtaa tgtttaccgg agaaatcagt gtagaataaa
     9361 cggatttttc cgtcagatgt aaatgtggct gaacctgacc attcttgtgt ttggtctttt
     9421 aggatagaat catttgcatc gaatttgtcg ctgtctttaa agacgcggcc agcgtttttc
     9481 cagctgtcaa tagaagtttc gccgactttt tgatagaaca tgtaaatcga tgtgtcatcc
     9541 gcatttttag gatctccggc taatgcaaag acgatgtggt agccgtgata gtttgcgaca
     9601 gtgccgtcag cgttttgtaa tggccagctg tcccaaacgt ccaggccttt tgcagaagag
     9661 atatttttaa ttgtggacga atcaaattca gaaacttgat atttttcatt tttttgctgt
     9721 tcagggattt gcagcatatc atggcgtgta atatgggaaa tgccgtatgt ttccttatat
     9781 ggcttttggt tcgtttcttt cgcaaacgct tgagttgcgc ctcctgccag cagtgcggta
     9841 gtaaaggtta atactgttgc ttgttttgca aactttttga tgttcatcgt tcatgtctcc
     9901 ttttttatgt actgtgttag cggtctgctt cttccagccc tcctgtttga agatggcaag
     9961 ttagttacgc acaataaaaa aagacctaaa atatgtaagg ggtgacgcca aagtatacac
    10021 tttgcccttt acacatttta ggtcttgcct gctttatcag taacaaaccc gcgcgattta
    10081 cttttcgacc tcattctatt agactctcgt ttggattgca actggtctat tttcctcttt
    10141 tgtttgatag aaaatcataa aaggatttgc agactacggg cctaaagaac taaaaaatct
    10201 atctgtttct tttcattctc tgtatttttt atagtttctg ttgcatgggc ataaagttgc
    10261 ctttttaatc acaattcaga aaatatcata atatctcatt tcactaaata atagtgaacg
    10321 gcaggtatat gtgggttacc gactgcggcc tgagtttttt aagtgacgta aaatcgtgtt
    10381 gaggccaacg cccataatgc gggctgttgc ccggcatcca acgccattca tggccatatc
    10441 aatgattttc tggtgcgtac cgggttgaga agcggtgtaa gtgaactgca ggtggcactt
    10501 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    10561 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    10621 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    10681 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    10741 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10801 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10861 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10921 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10981 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    11041 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    11101 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    11161 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    11221 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    11281 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    11341 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    11401 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    11461 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    11521 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
    11581 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
    11641 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
    11701 gagctgcatg tgtca
//
