LOCUS       62056_22425       11759 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            843..952
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             985..1353
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      1974..2362
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2477..3556)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(3557..3634)
                     /label=lacI promoter
     promoter        3680..3708
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             3755..4462
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      4499..4546
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5221..6012)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        6893..6911
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6912..6936
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             6980..8407
                     /codon_start=1
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     CDS             complement(8513..9931)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(9932..10377)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     promoter        10559..10663
                     /label=AmpR promoter
     CDS             10664..11521
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gaggttttca ccgtcatcac cgaaacgcgc gaggcagcaa ggagatggcg cccaacagtc
       61 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
      121 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
      181 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatccttttt gtccggtgtt
      241 gggttgaagg tgaagccggt cggggccgca gcgggggccg gcttttcagc cttgcccccc
      301 tgcttcggcc gccgtggctc cggcgtcttg ggtgccggcg cgggttccgc agccttggcc
      361 tgcggtgcgg gcacatcggc gggcttggcc ttgatgtgcc gcctggcgtg cgagcggaac
      421 gtctcgtagg agaacttgac cttccccgtt tcccgcatgt gctcccaaat ggtgacgagc
      481 gcatagccgg acgctaacgc cgcctcgaca tccgccctca ccgccaggaa cgcaaccgca
      541 gcctcatcac gccggcgctt cttggccgcg cgggattcaa cccactcggc cagctcgtcg
      601 gtgtagctct ttggcatcgt ctctcgcctg tcccctcagt tcagtaattt cctgcatttg
      661 cctgtttcca gtcggtagat attccacaaa acagcaggga agcagcgctt ttccgctgca
      721 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga
      781 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag
      841 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct
      901 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc
      961 ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc
     1021 ccacctatca aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg
     1081 gcggccggca tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg
     1141 ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc
     1201 ctgggcggcc tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat
     1261 gccacgatcc tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc
     1321 atgatgggcg tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg
     1381 gggggtgcgc gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc
     1441 ggagctggtg aagtacatca ccgacgagca aggcaagacc gagcgcctgg gtcacgtgcg
     1501 cgtcacgaac tgcgaggcaa acaccctgcc cgctgtcatg gccgaggtga tggcgaccca
     1561 gcacggcaac acccgttccg aggccgacaa gacctatcac ctgctggtta gcttccgcgc
     1621 gggagagaag cccgacgcgg agacgttgcg cgcgattgag gaccgcatct gcgctgggct
     1681 tggcttcgcc gagcatcagc gcgtcagtgc cgtgcatcac gacaccgaca acctgcacat
     1741 ccatatcgcc atcaacaaga ttcacccgac ccgaaacacc atccatgagc cgtatcgggc
     1801 ctaccgcgcc ctcgctgacc tctgcgcgac gctcgaacgg gactacgggc ttgagcgtga
     1861 caatcacgaa acgcggcagc gcgtttccga gaaccgcgcg aacgacatgg agcggcacgc
     1921 gggcgtggaa agcctggtcg gctggatccg gccacgatgc gtccggcgta gaggatctga
     1981 agatcagcag ttcaacctgt tgatagtacg tactaagctc tcatgtttca cgtactaagc
     2041 tctcatgttt aacgtactaa gctctcatgt ttaacgaact aaaccctcat ggctaacgta
     2101 ctaagctctc atggctaacg tactaagctc tcatgtttca cgtactaagc tctcatgttt
     2161 gaacaataaa attaatataa atcagcaact taaatagcct ctaaggtttt aagttttata
     2221 agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg tttaacggtt gtggacaaca
     2281 agccagggat gtaacgcact gagaagccct tagagcctct caaagcaatt ttgagtgaca
     2341 caggaacact taacggctga catgggaatt ccacatgtgg aattccacat gtggaattgt
     2401 gagcggataa caatttgtgg aattcccggg agagctcctg actcttatac acaagttcgg
     2461 ccacggccta ggctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2521 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc
     2581 ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt
     2641 gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta
     2701 acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatatccg
     2761 caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt
     2821 tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa
     2881 aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag
     2941 tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg
     3001 ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac
     3061 cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata
     3121 acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat
     3181 agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg
     3241 cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc
     3301 gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa
     3361 cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat
     3421 tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct
     3481 ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata
     3541 acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca
     3601 taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc
     3661 gactcctgca ttaggaaatt tgacaattaa tcatcggctc gtataatgtg tggaatgtct
     3721 catgagcgga tatttcacac aggaaacagt attcatggtg agcaagggcg aggaggataa
     3781 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     3841 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     3901 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     3961 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     4021 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     4081 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     4141 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     4201 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     4261 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     4321 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     4381 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     4441 cggcatggac gagctgtaca agtaattaac ctaggctgct gccaccgctg agcaataact
     4501 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttggcgg ccgcagcttg
     4561 tcgacgaatt cccggggatc cggtgattga ttgagcaagc tttatgcttg taaaccgttt
     4621 tgtgaaaaaa tttttaaaat aaaaaagggg acctctaggg tccccaatta attagtaata
     4681 taatctatta aaggtcattc aaaaggtcat ccaccggatc aattcccctg ctcacacaga
     4741 ctgggtacca agctctcggt aacatcaagg cccgatcctt ggagcccttg ccctcccgac
     4801 gatgatcgtg ccgtgatcga aatccagatc cttgacccac agttgcaaac cctcactgat
     4861 ccgtcccgtc cgggtcgacc aaagcggcca tcgtgcctcc ccactcctgc agttcggggg
     4921 catggatgcg cggatagccg ctgctggttt cctggatgcc gacggatttg cactgccggt
     4981 agaactccgc gaggtcgtcc agcctcaggc agcagctgaa ccaactcgcg aggggatcga
     5041 gcccggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg
     5101 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat
     5161 ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca
     5221 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     5281 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     5341 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     5401 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     5461 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     5521 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     5581 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     5641 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     5701 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     5761 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     5821 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     5881 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     5941 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     6001 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg
     6061 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
     6121 accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc
     6181 tagctatcgt catgtaagcc cactgcaagc tacctctttc tctttgcgct tgcgttttcc
     6241 cttgtccaga tagcccagta gctgacattc atccggggta gcaccgtttc tgcggactgg
     6301 ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgatgcttgc ggcagcgtga
     6361 agctcgcgca gatcagttgg ttgattttgt ccactacgtg aaaggcgaat caccaaggta
     6421 gtcggcaaat aatgtctaac aattcgttca agccgacgcc gcttcgcggc gcggttaact
     6481 caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtcgct
     6541 tttattattt ttaagcgtgc ataataagcc ctacacaaaa ttgggagata tatcatgaaa
     6601 ggcgcttttc ttgttatcgc aatagttggg cgaagtaatc gcaacatccg cattaacaat
     6661 ctagcgaggg cttggtaagg tgatccggtg gatgaccttt tgaatgacct ttaatagatt
     6721 atattactaa ttaattgggg accctagagg tccccttttt tattttaaaa attttttcac
     6781 aaaacggttt acaagcataa agcttgctca atcaatcacc ggatccccgg gaattcggcc
     6841 taggtggcca gatctgatca agagacagaa acgcaaaaga aaatgccgat attaatacga
     6901 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgttaact
     6961 ttaagaagga gatatacata tgattaccag tgcactgcat cgtgcggcgg attgggcgaa
     7021 aagcgtgttt tctagtgctg cgctgggtga tccgcgtcgt accgcgcgtc tggtgaatgt
     7081 tgcggcgcaa ctggccaaat atagcggcaa aagcattacc attagcagcg aaggcagcaa
     7141 agccatgcag gaaggcgcgt atcgttttat tcgtaatccg aacgtgagcg cggaagcgat
     7201 tcgtaaagcg ggtgccatgc agaccgtgaa actggcccag gaatttccgg aactgctggc
     7261 aattgaagat accacctctc tgagctatcg tcatcaggtg gcggaagaac tgggcaaact
     7321 gggtagcatt caggataaaa gccgtggttg gtgggtgcat agcgtgctgc tgctggaagc
     7381 gaccaccttt cgtaccgtgg gcctgctgca tcaagaatgg tggatgcgtc cggatgatcc
     7441 ggcggatgcg gatgaaaaag aaagcggcaa atggctggcc gctgctgcaa cttcgcgtct
     7501 gagaatgggc agcatgatga gcaacgtgat tgcggtgtgc gatcgtgaag cggatattca
     7561 tgcgtatctg caagataaac tggcccataa cgaacgtttt gtggtgcgta gcaaacatcc
     7621 gcgtaaagat gtggaaagcg gcctgtatct gtatgatcac ctgaaaaacc agccggaact
     7681 gggcggctat cagattagca ttccgcagaa aggcgtggtg gataaacgtg gcaaacgtaa
     7741 aaaccgtccg gcgcgtaaag cgagcctgag cctgcgtagc ggccgtatta ccctgaaaca
     7801 gggcaacatt accctgaacg cggtgctggc cgaagaaatt aatccgccga aaggcgaaac
     7861 cccgctgaaa tggctgctgc tgaccagcga gccggtggaa agtctggccc aagcgctgcg
     7921 tgtgattgat atttataccc atcgttggcg cattgaagaa tttcacaaag cgtggaaaac
     7981 gggtgcgggt gcggaacgtc agcgtatgga agaaccggat aacctggaac gtatggtgag
     8041 cattctgagc tttgtggcgg tgcgtctgct gcaactgcgt gaatctttta ctccgccgca
     8101 agcactgcgt gcgcagggcc tgctgaaaga agcggaacac gttgaaagcc agagcgcgga
     8161 aaccgtgctg accccggatg aatgccaact gctgggctat ctggataaag gcaaacgcaa
     8221 acgcaaagaa aaagcgggca gcctgcaatg ggcgtatatg gcgattgcgc gtctgggcgg
     8281 ctttatggat agcaaacgta ccggcattgc gagctggggt gcgctgtggg aaggttggga
     8341 agcgctgcaa agcaaactgg atggctttct ggccgcgaaa gacctgatgg cgcagggcat
     8401 taaaatctaa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgac
     8461 gcgtgctatc gtcatgtaag cccactgatc ggcattttct tttgcgtttt tatttgttaa
     8521 ctgttaattg tccttgttca aggatgctgt ctttgacaac agatgttttc ttgcctttga
     8581 tgttcagcag gaagctcggc gcaaacgttg attgtttgtc tgcgtagaat cctctgtttg
     8641 tcatatagct tgtaatcacg acattgtttc ctttcgcttg aggtacagcg aagtgtgagt
     8701 aagtaaaggt tacatcgtta ggatcaagat ccatttttaa cacaaggcca gttttgttca
     8761 gcggcttgta tgggccagtt aaagaattag aaacataacc aagcatgtaa atatcgttag
     8821 acgtaatgcc gtcaatcgtc atttttgatc cgcgggagtc agtgaacagg taccatttgc
     8881 cgttcatttt aaagacgttc gcgcgttcaa tttcatctgt tactgtgtta gatgcaatca
     8941 gcggtttcat cacttttttc agtgtgtaat catcgtttag ctcaatcata ccgagagcgc
     9001 cgtttgctaa ctcagccgtg cgttttttat cgctttgcag aagtttttga ctttcttgac
     9061 ggaagaatga tgtgcttttg ccatagtatg ctttgttaaa taaagattct tcgccttggt
     9121 agccatcttc agttccagtg tttgcttcaa atactaagta tttgtggcct ttatcttcta
     9181 cgtagtgagg atctctcagc gtatggttgt cgcctgagct gtagttgcct tcatcgatga
     9241 actgctgtac attttgatac gtttttccgt caccgtcaaa gattgattta taatcctcta
     9301 caccgttgat gttcaaagag ctgtctgatg ctgatacgtt aacttgtgca gttgtcagtg
     9361 tttgtttgcc gtaatgttta ccggagaaat cagtgtagaa taaacggatt tttccgtcag
     9421 atgtaaatgt ggctgaacct gaccattctt gtgtttggtc ttttaggata gaatcatttg
     9481 catcgaattt gtcgctgtct ttaaagacgc ggccagcgtt tttccagctg tcaatagaag
     9541 tttcgccgac tttttgatag aacatgtaaa tcgatgtgtc atccgcattt ttaggatctc
     9601 cggctaatgc aaagacgatg tggtagccgt gatagtttgc gacagtgccg tcagcgtttt
     9661 gtaatggcca gctgtcccaa acgtccaggc cttttgcaga agagatattt ttaattgtgg
     9721 acgaatcaaa ttcagaaact tgatattttt catttttttg ctgttcaggg atttgcagca
     9781 tatcatggcg tgtaatatgg gaaatgccgt atgtttcctt atatggcttt tggttcgttt
     9841 ctttcgcaaa cgcttgagtt gcgcctcctg ccagcagtgc ggtagtaaag gttaatactg
     9901 ttgcttgttt tgcaaacttt ttgatgttca tcgttcatgt ctcctttttt atgtactgtg
     9961 ttagcggtct gcttcttcca gccctcctgt ttgaagatgg caagttagtt acgcacaata
    10021 aaaaaagacc taaaatatgt aaggggtgac gccaaagtat acactttgcc ctttacacat
    10081 tttaggtctt gcctgcttta tcagtaacaa acccgcgcga tttacttttc gacctcattc
    10141 tattagactc tcgtttggat tgcaactggt ctattttcct cttttgtttg atagaaaatc
    10201 ataaaaggat ttgcagacta cgggcctaaa gaactaaaaa atctatctgt ttcttttcat
    10261 tctctgtatt ttttatagtt tctgttgcat gggcataaag ttgccttttt aatcacaatt
    10321 cagaaaatat cataatatct catttcacta aataatagtg aacggcaggt atatgtgggt
    10381 taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc aacgcccata
    10441 atgcgggctg ttgcccggca tccaacgcca ttcatggcca tatcaatgat tttctggtgc
    10501 gtaccgggtt gagaagcggt gtaagtgaac tgcaggtggc acttttcggg gaaatgtgcg
    10561 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    10621 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
    10681 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
    10741 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
    10801 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    10861 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
    10921 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    10981 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    11041 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    11101 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    11161 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
    11221 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    11281 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    11341 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    11401 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    11461 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    11521 gtaactgtca gaccaagttt actcatatat actttagatt gatttatggt gcactctcag
    11581 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
    11641 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
    11701 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtca
//
