LOCUS       62056_22415       10278 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            843..952
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             985..1353
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      1974..2362
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     promoter        2480..2508
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     RBS             2523..2545
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2553..3269
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      3280..3326
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(4001..4531)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        5412..5430
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5431..5455
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             5499..6926
                     /codon_start=1
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     terminator      6930..6977
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(7032..8450)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(8451..8896)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     promoter        9078..9182
                     /label=AmpR promoter
     CDS             9183..10040
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gaggttttca ccgtcatcac cgaaacgcgc gaggcagcaa ggagatggcg cccaacagtc
       61 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
      121 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
      181 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatccttttt gtccggtgtt
      241 gggttgaagg tgaagccggt cggggccgca gcgggggccg gcttttcagc cttgcccccc
      301 tgcttcggcc gccgtggctc cggcgtcttg ggtgccggcg cgggttccgc agccttggcc
      361 tgcggtgcgg gcacatcggc gggcttggcc ttgatgtgcc gcctggcgtg cgagcggaac
      421 gtctcgtagg agaacttgac cttccccgtt tcccgcatgt gctcccaaat ggtgacgagc
      481 gcatagccgg acgctaacgc cgcctcgaca tccgccctca ccgccaggaa cgcaaccgca
      541 gcctcatcac gccggcgctt cttggccgcg cgggattcaa cccactcggc cagctcgtcg
      601 gtgtagctct ttggcatcgt ctctcgcctg tcccctcagt tcagtaattt cctgcatttg
      661 cctgtttcca gtcggtagat attccacaaa acagcaggga agcagcgctt ttccgctgca
      721 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga
      781 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag
      841 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct
      901 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc
      961 ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc
     1021 ccacctatca aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg
     1081 gcggccggca tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg
     1141 ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc
     1201 ctgggcggcc tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat
     1261 gccacgatcc tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc
     1321 atgatgggcg tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg
     1381 gggggtgcgc gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc
     1441 ggagctggtg aagtacatca ccgacgagca aggcaagacc gagcgcctgg gtcacgtgcg
     1501 cgtcacgaac tgcgaggcaa acaccctgcc cgctgtcatg gccgaggtga tggcgaccca
     1561 gcacggcaac acccgttccg aggccgacaa gacctatcac ctgctggtta gcttccgcgc
     1621 gggagagaag cccgacgcgg agacgttgcg cgcgattgag gaccgcatct gcgctgggct
     1681 tggcttcgcc gagcatcagc gcgtcagtgc cgtgcatcac gacaccgaca acctgcacat
     1741 ccatatcgcc atcaacaaga ttcacccgac ccgaaacacc atccatgagc cgtatcgggc
     1801 ctaccgcgcc ctcgctgacc tctgcgcgac gctcgaacgg gactacgggc ttgagcgtga
     1861 caatcacgaa acgcggcagc gcgtttccga gaaccgcgcg aacgacatgg agcggcacgc
     1921 gggcgtggaa agcctggtcg gctggatccg gccacgatgc gtccggcgta gaggatctga
     1981 agatcagcag ttcaacctgt tgatagtacg tactaagctc tcatgtttca cgtactaagc
     2041 tctcatgttt aacgtactaa gctctcatgt ttaacgaact aaaccctcat ggctaacgta
     2101 ctaagctctc atggctaacg tactaagctc tcatgtttca cgtactaagc tctcatgttt
     2161 gaacaataaa attaatataa atcagcaact taaatagcct ctaaggtttt aagttttata
     2221 agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg tttaacggtt gtggacaaca
     2281 agccagggat gtaacgcact gagaagccct tagagcctct caaagcaatt ttgagtgaca
     2341 caggaacact taacggctga catgggaatt ccacatgtgg aattccacat gtggaattgt
     2401 gagcggataa caatttgtgg aattcccggg agagctcctg actcttatac acaagttcgg
     2461 ccacggccta ggcggccgct tgacaattaa tcatcggctc gtataatgcc tctagaaata
     2521 attttgttta actttaagaa ggagataccg gtatggtgag caagggcgag gagctgttca
     2581 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     2641 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     2701 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     2761 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     2821 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2881 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2941 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3001 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     3061 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3121 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     3181 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     3241 tcactctcgg catggacgag ctgtacaagt agggagctcc aaataaaacg aaaagctcag
     3301 tcgaaagact gggcctttcg ttttatgcgg ccgcagcttg tcgacgaatt cccggggatc
     3361 cggtgattga ttgagcaagc tttatgcttg taaaccgttt tgtgaaaaaa tttttaaaat
     3421 aaaaaagggg acctctaggg tccccaatta attagtaata taatctatta aaggtcattc
     3481 aaaaggtcat ccaccggatc aattcccctg ctcacacaga ctgggtacca agctctcggt
     3541 aacatcaagg cccgatcctt ggagcccttg ccctcccgac gatgatcgtg ccgtgatcga
     3601 aatccagatc cttgacccac agttgcaaac cctcactgat ccgtcccgtc cgggtcgacc
     3661 aaagcggcca tcgtgcctcc ccactcctgc agttcggggg catggatgcg cggatagccg
     3721 ctgctggttt cctggatgcc gacggatttg cactgccggt agaactccgc gaggtcgtcc
     3781 agcctcaggc agcagctgaa ccaactcgcg aggggatcga gcccggggtg ggcgaagaac
     3841 tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg
     3901 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc
     3961 gtcgcttggt cggtcatttc gaaccccaga gtcccgctta ggtggcggta cttgggtcga
     4021 tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat
     4081 cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct
     4141 tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga
     4201 gatcatagat atagatctca ctacgcggct gctcaaacct gggcagaacg taagccgcga
     4261 gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga
     4321 gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga
     4381 actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc
     4441 tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct
     4501 gctgcgtaac atcgttgctg ctgcgtaaca tgcgaaacga tcctcatcct gtctcttgat
     4561 cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     4621 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
     4681 tccataaaac cgcccagtct agctatcgtc atgtaagccc actgcaagct acctctttct
     4741 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtag
     4801 caccgtttct gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct
     4861 gatgcttgcg gcagcgtgaa gctcgcgcag atcagttggt tgattttgtc cactacgtga
     4921 aaggcgaatc accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg
     4981 cttcgcggcg cggttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     5041 actatcaggt caagtcgctt ttattatttt taagcgtgca taataagccc tacacaaaat
     5101 tgggagatat atcatgaaag gcgcttttct tgttatcgca atagttgggc gaagtaatcg
     5161 caacatccgc attaacaatc tagcgagggc ttggtaaggt gatccggtgg atgacctttt
     5221 gaatgacctt taatagatta tattactaat taattgggga ccctagaggt cccctttttt
     5281 attttaaaaa ttttttcaca aaacggttta caagcataaa gcttgctcaa tcaatcaccg
     5341 gatccccggg aattcggcct aggtggccag atctgatcaa gagacagaaa cgcaaaagaa
     5401 aatgccgata ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
     5461 agaaataatt ttgttaactt taagaaggag atatacatat gattaccagt gcactgcatc
     5521 gtgcggcgga ttgggcgaaa agcgtgtttt ctagtgctgc gctgggtgat ccgcgtcgta
     5581 ccgcgcgtct ggtgaatgtt gcggcgcaac tggccaaata tagcggcaaa agcattacca
     5641 ttagcagcga aggcagcaaa gccatgcagg aaggcgcgta tcgttttatt cgtaatccga
     5701 acgtgagcgc ggaagcgatt cgtaaagcgg gtgccatgca gaccgtgaaa ctggcccagg
     5761 aatttccgga actgctggca attgaagata ccacctctct gagctatcgt catcaggtgg
     5821 cggaagaact gggcaaactg ggtagcattc aggataaaag ccgtggttgg tgggtgcata
     5881 gcgtgctgct gctggaagcg accacctttc gtaccgtggg cctgctgcat caagaatggt
     5941 ggatgcgtcc ggatgatccg gcggatgcgg atgaaaaaga aagcggcaaa tggctggccg
     6001 ctgctgcaac ttcgcgtctg agaatgggca gcatgatgag caacgtgatt gcggtgtgcg
     6061 atcgtgaagc ggatattcat gcgtatctgc aagataaact ggcccataac gaacgttttg
     6121 tggtgcgtag caaacatccg cgtaaagatg tggaaagcgg cctgtatctg tatgatcacc
     6181 tgaaaaacca gccggaactg ggcggctatc agattagcat tccgcagaaa ggcgtggtgg
     6241 ataaacgtgg caaacgtaaa aaccgtccgg cgcgtaaagc gagcctgagc ctgcgtagcg
     6301 gccgtattac cctgaaacag ggcaacatta ccctgaacgc ggtgctggcc gaagaaatta
     6361 atccgccgaa aggcgaaacc ccgctgaaat ggctgctgct gaccagcgag ccggtggaaa
     6421 gtctggccca agcgctgcgt gtgattgata tttataccca tcgttggcgc attgaagaat
     6481 ttcacaaagc gtggaaaacg ggtgcgggtg cggaacgtca gcgtatggaa gaaccggata
     6541 acctggaacg tatggtgagc attctgagct ttgtggcggt gcgtctgctg caactgcgtg
     6601 aatcttttac tccgccgcaa gcactgcgtg cgcagggcct gctgaaagaa gcggaacacg
     6661 ttgaaagcca gagcgcggaa accgtgctga ccccggatga atgccaactg ctgggctatc
     6721 tggataaagg caaacgcaaa cgcaaagaaa aagcgggcag cctgcaatgg gcgtatatgg
     6781 cgattgcgcg tctgggcggc tttatggata gcaaacgtac cggcattgcg agctggggtg
     6841 cgctgtggga aggttgggaa gcgctgcaaa gcaaactgga tggctttctg gccgcgaaag
     6901 acctgatggc gcagggcatt aaaatctaac tagcataacc ccttggggcc tctaaacggg
     6961 tcttgagggg ttttttgacg cgtgctatcg tcatgtaagc ccactgatcg gcattttctt
     7021 ttgcgttttt atttgttaac tgttaattgt ccttgttcaa ggatgctgtc tttgacaaca
     7081 gatgttttct tgcctttgat gttcagcagg aagctcggcg caaacgttga ttgtttgtct
     7141 gcgtagaatc ctctgtttgt catatagctt gtaatcacga cattgtttcc tttcgcttga
     7201 ggtacagcga agtgtgagta agtaaaggtt acatcgttag gatcaagatc catttttaac
     7261 acaaggccag ttttgttcag cggcttgtat gggccagtta aagaattaga aacataacca
     7321 agcatgtaaa tatcgttaga cgtaatgccg tcaatcgtca tttttgatcc gcgggagtca
     7381 gtgaacaggt accatttgcc gttcatttta aagacgttcg cgcgttcaat ttcatctgtt
     7441 actgtgttag atgcaatcag cggtttcatc acttttttca gtgtgtaatc atcgtttagc
     7501 tcaatcatac cgagagcgcc gtttgctaac tcagccgtgc gttttttatc gctttgcaga
     7561 agtttttgac tttcttgacg gaagaatgat gtgcttttgc catagtatgc tttgttaaat
     7621 aaagattctt cgccttggta gccatcttca gttccagtgt ttgcttcaaa tactaagtat
     7681 ttgtggcctt tatcttctac gtagtgagga tctctcagcg tatggttgtc gcctgagctg
     7741 tagttgcctt catcgatgaa ctgctgtaca ttttgatacg tttttccgtc accgtcaaag
     7801 attgatttat aatcctctac accgttgatg ttcaaagagc tgtctgatgc tgatacgtta
     7861 acttgtgcag ttgtcagtgt ttgtttgccg taatgtttac cggagaaatc agtgtagaat
     7921 aaacggattt ttccgtcaga tgtaaatgtg gctgaacctg accattcttg tgtttggtct
     7981 tttaggatag aatcatttgc atcgaatttg tcgctgtctt taaagacgcg gccagcgttt
     8041 ttccagctgt caatagaagt ttcgccgact ttttgataga acatgtaaat cgatgtgtca
     8101 tccgcatttt taggatctcc ggctaatgca aagacgatgt ggtagccgtg atagtttgcg
     8161 acagtgccgt cagcgttttg taatggccag ctgtcccaaa cgtccaggcc ttttgcagaa
     8221 gagatatttt taattgtgga cgaatcaaat tcagaaactt gatatttttc atttttttgc
     8281 tgttcaggga tttgcagcat atcatggcgt gtaatatggg aaatgccgta tgtttcctta
     8341 tatggctttt ggttcgtttc tttcgcaaac gcttgagttg cgcctcctgc cagcagtgcg
     8401 gtagtaaagg ttaatactgt tgcttgtttt gcaaactttt tgatgttcat cgttcatgtc
     8461 tcctttttta tgtactgtgt tagcggtctg cttcttccag ccctcctgtt tgaagatggc
     8521 aagttagtta cgcacaataa aaaaagacct aaaatatgta aggggtgacg ccaaagtata
     8581 cactttgccc tttacacatt ttaggtcttg cctgctttat cagtaacaaa cccgcgcgat
     8641 ttacttttcg acctcattct attagactct cgtttggatt gcaactggtc tattttcctc
     8701 ttttgtttga tagaaaatca taaaaggatt tgcagactac gggcctaaag aactaaaaaa
     8761 tctatctgtt tcttttcatt ctctgtattt tttatagttt ctgttgcatg ggcataaagt
     8821 tgccttttta atcacaattc agaaaatatc ataatatctc atttcactaa ataatagtga
     8881 acggcaggta tatgtgggtt accgactgcg gcctgagttt tttaagtgac gtaaaatcgt
     8941 gttgaggcca acgcccataa tgcgggctgt tgcccggcat ccaacgccat tcatggccat
     9001 atcaatgatt ttctggtgcg taccgggttg agaagcggtg taagtgaact gcaggtggca
     9061 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9121 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9181 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9241 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9301 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9361 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9421 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9481 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9541 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     9601 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9661 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9721 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     9781 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     9841 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     9901 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     9961 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10021 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10081 atttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
    10141 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
    10201 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
    10261 cgggagctgc atgtgtca
//
