LOCUS       40924_45798        5935 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mini Tn5 transposon vector pUT-dfrA17, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Tsai Y, Chu C, Lee C-H., Liaw S-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Department of Clinical Laboratory Sciences 
            and Medical Biotechnology, College of Medicine, National Taiwan 
            University, No. 1, Chang-Te St., Taipei 100, Taiwan
REFERENCE   2  (bases 1 to 5935)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5935)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2013) Department of Clinical Laboratory Sciences and Medical
            Biotechnology, College of Medicine, National Taiwan University, No. 
            1, Chang-Te St., Taipei 100, Taiwan"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(314..1171)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1172..1276)
                     /label=AmpR promoter
     CDS             complement(1326..2753)
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
     misc_feature    2871..2889
                     /note="Tn5 inside end; IE"
     CDS             3007..3555
                     /codon_start=1
                     /gene="dfrA17"
                     /product="dihydrofolate reductase DfrA17"
                     /label=dfrA17
                     /protein_id="AHG52772.1"
                     /translation="MLWSSNDVTQQGSRPKTKLAIKGVKLKISLISAVSENGVIGSGPD
                     IPWSVKGEQLLFKALTYNQWLLVGRKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPS
                     IENALKELSKVTDHVYVSGGGQIYNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLV
                     FEQFFMSNINYTYQIWKKG"
     gene            3007..3555
                     /gene="dfrA17"
                     /label=dfrA17
     misc_feature    3568..3586
                     /note="Tn5 outside end; OE"
     protein_bind    complement(3598..3614)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(3649..4037)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(4658..5026)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(5059..5168)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gccccgtggc cgggggactg ttgggcgcca tctccttgct gcctcgcgcg tttcggtgat
       61 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      181 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
      241 cagagcagat tgtactgaga gtgcaccata aatcaatcta aagtatatat gagtaaactt
      301 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
      361 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
      421 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
      481 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
      541 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
      601 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
      661 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
      721 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
      781 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
      841 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
      901 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
      961 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1021 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1081 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     1141 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     1201 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     1261 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcagatctg caggtcgacc
     1321 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
     1381 ctttgcaggg cttcccaacc ttcccagagg gcgccccagc tggcaattcc ggttcgcttg
     1441 ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct
     1501 ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg
     1561 gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc
     1621 gccctgagtg cttgcggcag cgtgaagctt tctctgagct gtaacagcct gaccgcaaca
     1681 aacgagagga tcgagaccat ccgctccaga ttatccggct cctccatgcg ttgcctctcg
     1741 gctcctgctc cggttttcca tgccttatgg aactcctcga tccgccagcg atgggtataa
     1801 atgtcgatga cgcgcaaggc ttgggctagc gactcgaccg gttcgctggt cagcaacaac
     1861 catttcaacg gggtctcacc cttgggcggg ttaatctcct cggccagcac cgcgttgagc
     1921 gtgatattcc cctgttttag cgtgatgcgc ccactgcgca ggctcaagct cgccttgcgg
     1981 gctggtcgat ttttacgttt accgcgttta tccaccacgc ccttttgcgg aatgctgatc
     2041 tgatagccac ccaactccgg ttggttcttc agatggtcgt acagatacaa cccagactct
     2101 acgtccttgc gtgggtgctt ggagcgcacc acgaagcgct cgttatgcgc cagtttgtcc
     2161 tgcagataag catgaatatc ggcttcgcgg tcacagaccg caatcacgtt gctcatcatg
     2221 ctgcccatgc gtaaccggct agttgcggcg gctgccagcc atttgccact ctccttttca
     2281 tccgcatcgg cagggtcatc cgggcgcatc caccactcct gatgcagtaa tcctacggtg
     2341 cggaatgtgg tggcctcgag caagagaacg gagtgaaccc accatccgcg ggatttatcc
     2401 tgaatagagc ccagcttgcc aagctcttcg gcgacctggt ggcgataact caaagaggtg
     2461 gtgtcctcaa tggccagcag ttcgggaaac tcctgagcca acttgactgt ttgcatggcg
     2521 ccagcctttc tgatcgcctc ggcagaaacg ttgggattgc ggataaatcg gtaagcgcct
     2581 tcctgcatgg cttcactacc ctctgatgag atggttattg atttaccaga atattttgcc
     2641 aattgggcgg cgacgttaac caagcgggca gtacggcgag gatcacccag cgccgccgaa
     2701 gagaacacag atttagccca gtcggccgca cgatgaagag cagaagttat catgaacgtt
     2761 accatgttag gaggtcacat ggaagatcag atcctggaaa acgggaaagg ttccgttcag
     2821 gacgctactt gtgtacggga tcggtcgacg gatcccaagc ttcttctaga ctgtctcttg
     2881 atcagatctg gccgcctagg ccgaattcga gctcggtacc tcatggcttg ttatgactgt
     2941 ttttttgtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     3001 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     3061 ttagccatta agggggttaa attgaaaata tcattgattt ctgcagtgtc agaaaatggc
     3121 gtaatcggta gtggtcctga tatcccgtgg tcagtaaaag gtgagcaact actctttaaa
     3181 gcgctcacat ataatcaatg gctccttgtc ggaagaaaaa catttgactc tatgggtgtt
     3241 cttccaaatc gcaaatatgc agtagtgtca aagaacggaa tttcaagctc aaatgaaaac
     3301 gtcctggttt ttccttcaat agaaaatgct ttgaaagagc tatcaaaagt tacagatcat
     3361 gtatatgtct ctggcggggg tcaaatctat aatagcctta ttgaaaaagc agatataatt
     3421 catttgtcta ctgttcacgt tgaagtcgaa ggtgatatca aattccctat aatgcctgag
     3481 aatttcaatt tggtttttga acagtttttt atgtctaata taaattatac ataccagatt
     3541 tggaaaaaag gctaacaatg cggccgcact tgtgtataag agtcagaatt ccacaaattg
     3601 ttatccgctc acaattccac atgtggaatt ccacatgtgg aattcccatg tcagccgtta
     3661 agtgttcctg tgtcactcaa aattgctttg agaggctcta agggcttctc agtgcgttac
     3721 atccctggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc
     3781 ttttttttct tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat
     3841 tttattgttc aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat
     3901 gagagcttag tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt
     3961 aaacatgaga gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa
     4021 ctgctgatct tcagatcctc tacgccggac gcatcgtggc cggatccagc cgaccaggct
     4081 ttccacgccc gcgtgccgct ccatgtcgtt cgcgcggttc tcggaaacgc gctgccgcgt
     4141 ttcgtgattg tcacgctcaa gcccgtagtc ccgttcgagc gtcgcgcaga ggtcagcgag
     4201 ggcgcggtag gcccgatacg gctcatggat ggtgtttcgg gtcgggtgaa tcttgttgat
     4261 ggcgatatgg atgtgcaggt tgtcggtgtc gtgatgcacg gcactgacgc gctgatgctc
     4321 ggcgaagcca agcccagcgc agatgcggtc ctcaatcgcg cgcaacgtct ccgcgtcggg
     4381 cttctctccc gcgcggaagc taaccagcag gtgataggtc ttgtcggcct cggaacgggt
     4441 gttgccgtgc tgggtcgcca tcacctcggc catgacagcg ggcagggtgt ttgcctcgca
     4501 gttcgtgacg cgcacgtgac ccaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt
     4561 caccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac
     4621 gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca
     4681 cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga
     4741 ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca
     4801 ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt
     4861 ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca
     4921 tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct
     4981 tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat
     5041 ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca
     5101 ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta
     5161 tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa
     5221 atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga
     5281 agcagggtta tgcagcggaa aagcgctgct tccctgctgt tttgtggaat atctaccgac
     5341 tggaaacagg caaatgcagg aaattactga actgagggga caggcgagag acgatgccaa
     5401 agagctacac cgacgagctg gccgagtggg ttgaatcccg cgcggccaag aagcgccggc
     5461 gtgatgaggc tgcggttgcg ttcctggcgg tgagggcgga tgtcgaggcg gcgttagcgt
     5521 ccggctatgc gctcgtcacc atttgggagc acatgcggga aacggggaag gtcaagttct
     5581 cctacgagac gttccgctcg cacgccaggc ggcacatcaa ggccaagccc gccgatgtgc
     5641 ccgcaccgca ggccaaggct gcggaacccg cgccggcacc caagacgccg gagccacggc
     5701 ggccgaagca ggggggcaag gctgaaaagc cggcccccgc tgcggccccg accggcttca
     5761 ccttcaaccc aacaccggac aaaaaggatc ctctacgccg gacgcatcgt ggccggcatc
     5821 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     5881 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcag
//
